早发冠心病患者甲烯四氢叶酸还原酶基因的C677T多态性研究

目的探讨同型半胱氨酸代谢过程的关键酶之一——甲烯四氢叶酸还原酶（ＭＴＨＦＲ）基因Ｃ６７７Ｔ多态性与早发冠心病发病的关系。方法采用限制性内切酶片段长度多态性方法检测患者的ＭＴＨＦＲ基因Ｃ６７７Ｔ位碱基突变。结果６７例患者中Ｔ纯合基因型占３４．３％（２３／６７）、杂合基因型占４３．３％（２９／６７）,Ｃ纯合基因型占２２．４％（１５／６７）;Ｔ等位基因频率为５５．９％（７５／１３４）,Ｃ等位基因频率为４４．１％（５９／１３４）;与正常对照组比较,差异均有显著性意义（Ｐ＜０．０５）。结论ＭＴＨＦＲ基因Ｃ６７７Ｔ点突变可能是中国人早发冠心病发病的危险因素之一。     

亚甲基四氢叶酸还原酶基因多态性与原发性高血压的关系

目的：探讨上海社区人群亚甲基四氢叶酸还原酶（ｍｅｔｈｙｌｅｎｅｔｅｔｒａｈｙｄｒｏｆｏｌａｔｅｒｅｄｕｃｔａｓｅ,ＭＴＨＦＲ）基因多态性与原发性高血压的关系。方法：用聚合酶链反应限制性片段的多态性（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍ,ＰＣＲＲＦＬＰ）技术,检测１２７例原发性高血压及１７０例正常对照的ＭＴＨＦＲ基因多态性。结果：ＭＴＨＦＲ基因第６７７位核苷酸呈多态性,可分为３种类型：Ｃ／Ｃ、Ｔ／Ｔ、Ｃ／Ｔ。原发性高血压病例组３种基因型频率为：Ｃ／Ｃ,３４．６％;Ｃ／Ｔ,５３．５％;Ｔ／Ｔ,１１．８％。对照组中分别为３６．５％;４９．４％;１４．１％。病例组与对照组Ｔ／Ｔ基因型频率无显著性差异。结论：在上海社区人群中,ＭＴＨＦＲ基因多态性可能不是原发性高血压的危险因素。     

PCR—RFLP法检测N^5,N^10—亚甲基四氢叶酸还原酶基因多态性及其 …

目的：采用ＰＣＲ－ＲＦＬＰ方法检测Ｎ＾５,Ｎ＾１０－亚甲基四氢叶酸还原酶基因多态性,分析该基因的Ｃ６７７→Ｔ突变与心脑血管栓塞的关系。方法：应用一对特异引物进行扩增,扩增片段长度为１９８ｂｐ,再以ＨｉｍｆⅠ酶切,经６％聚丙烯酰胺凝胶电泳分析ＭＴＨＦＲ基因型。结果：３１例脑梗塞患者中＋／＋型５例（１６．１５％）,＋／－型１７例（５４．８５％）,－／－型９例（２９．１％）;２６例心肌梗塞者中＋／＋型５     

冠心病患者血浆同型半胱氨酸的变化及其机制的探讨

目的探讨同型半胱氨酸（ＨＣＹ）及其代谢相关因子和血脂与冠心病发病之间的关系。方法４０例经冠状动脉造影术证实为正常或冠心病患者，应用高效液相色谱分析测定血浆ＨＣＹ，同时测定血浆叶酸、维生素Ｂ１２浓度及血脂水平，并应用ＰＣＲ检测Ｎ５，Ｎ１０亚甲基四氢叶酸还原酶（ＭＴＨＦＲ）基因型。结果（１）冠心病患者血浆ＨＣＹ浓度（１７１±３６μｍｏｌ／Ｌ）高于正常对照组（７６±１２μｍｏｌ／Ｌ），合并有心肌梗死、高血压和有冠心病家族史的患者，其血浆ＨＣＹ浓度更高；（２）血浆ＨＣＹ浓度与血浆叶酸、维生素Ｂ１２浓度呈明显的非线性相关；（３）ＭＴＨＦＲ纯合子突变者，血浆ＨＣＹ浓度高；（４）ＣＨＤ患者血脂与ＨＣＹ水平无明显相关。结论高同型半胱氨酸血症是冠心病的新的独立危险因素。     

亚甲基四氢叶酸还原酶基因多态性与原发性高血压的关系

目的：探讨上海社区人群亚甲基四氢叶酸还原酶基因多态性与原发性高血压的关系。方法：用聚合酶链反应－限制性片段的多态性技术,检测１２７例原发性高血压及１７０例正常对照的ＭＴＨＦＲ基因多态性。结果：ＭＴＨＦＲ基因第６７７位核苷酸呈多态性,可分为３种类型,Ｃ／Ｃ,Ｔ／Ｔ,Ｃ／Ｔ。原发性高血压病例组３种基因型频率为：Ｃ／Ｃ,３４．６＾,Ｃ／Ｔ,５３．５％,Ｔ／Ｔ,１１．８＾。对照组中分别为３６．５％;４９．     

Ｈ 型高血压合并冠心病 ＭＴＨＦＲ 基因 Ｃ６７７Ｔ 多态性 与冠状动脉病变的分析

目的：探讨 Ｈ 型高血压合并冠心病病人亚甲基四氢叶酸还原酶（ＭＴＨＦＲ）Ｃ６７７Ｔ 基因多态性、血清 同型半胱氨酸（Ｈｃｙ）与冠状动脉病变的关系。方法：选取 ２０１７－１０ ～ ２０１８－１１ 期间我院心内科诊断为 Ｈ 型高血 压合并冠心病病人 １２０ 例作为试验组,其中合并稳定型心绞痛（ＳＡＰ）４０ 例为 Ａ 组,合并不稳定型心绞痛（ＵＡＰ） ４２ 例为 Ｂ 组,合并急性心肌梗死（ＡＭＩ）３８ 例为 Ｃ 组,Ｈ 型高血压病人 ４０ 例作为对照 Ｄ 组,检测 ＭＴＨＦＲ Ｃ６７７ Ｔ 基因、血清同型半胱氨酸（Ｈｃｙ）,根据冠状动脉造影结果采用 Ｇｅｎｓｉｎｉ 评分法评估冠状动脉病变程度。结果：Ｈ 型高血压合并冠心病组 Ｈｃｙ ２５．３４±９．２５μｍｏｌ ／ Ｌ 较 Ｈ 型高血压组１６．４６±８．１２μｍｏｌ ／ Ｌ 明显增高。ＴＴ 型 Ｈｃｙ ３２．１４± １２．４４μｍｏｌ ／ Ｌ 较 ＣＴ 型２３．２６±１０．２７μｍｏｌ ／ Ｌ、ＣＣ 型１４．５２±８．２３μｍｏｌ ／ Ｌ 明显增高。Ｈ 型高血压合并冠心病组 ＴＴ 型 （３３％）、Ｔ 等位基因频率（５４％）较 Ｈ 型高血压组 ＴＴ 型（１８％）、Ｔ 等位基因频率（４０％）明显增高。ＴＴ 型 Ｇｅｎｓｉｎｉ 评分４１．５６± ４．４８明显高于 ＣＣ 型２８．４２ ± ３．２３,有统计学意义（Ｐ＜ ０．０５）。Ｂ 组 ＴＴ 型（３６％）、Ｔ 等位基因频率 （５６％）、Ｃ 组 ＴＴ 型（３７％）、Ｔ 等位基因频率（５８％）较 Ａ 组 ＴＴ 型（２８％）、Ｔ 等位基因频率（４９％）增高,有统计学 意义（Ｐ＜０．０５）。Ｂ 组、Ｃ 组 ＴＴ 型、Ｔ 等位基因频率无统计学意义（Ｐ＞０．０５）。结论：Ｈ 型高血压合并冠心病组 Ｈｃｙ 水平、ＴＴ 型、Ｔ 等位基因频率较 Ｈ 型高血压组明显增高。ＴＴ 型 Ｈｃｙ、Ｇｅｎｓｉｎｉ 评分明显增高。Ｂ 组、Ｃ 组 ＴＴ 型、Ｔ 等位基因频率较 Ａ 组增高,Ｂ、Ｃ 组 ＴＴ 型、Ｔ 等位基因频率无统计学意义。     

血浆同型半胱氨酸和叶酸及ＭＴＨＦＲ基因多态性与早产

【目的】 探讨孕妇血浆同型半胱氨酸水平（Ｈｃｙ）、叶酸水平及ＭＴＨＦＲ基因多态性与早产的相关性。【方法】 采用病例对照研究,选取４４０例分娩孕妇为研究对象,其中早产临产孕妇（ｃａｓｅ ｇｒｏｕｐ）、同孕周健康孕妇（ｃｏｎｔｒｏｌ ｇｒｏｕｐ）各２２０例。用高效液相色谱荧光检测法（ＨＰＬＣ－ＦＤ）检测两组孕妇血浆同型半胱氨酸水平和叶酸水平差异;１４Ｃ 放射法测定两组孕妇ＭＴＨＦＲ的活性;Ｔａｑｍａｎ－ＭＧＢ技术检测两组孕妇ＭＴＨＦＲ基因的５个ＳＮＰ分型差异。【结果】 早产临产孕妇的血浆Ｈｃｙ 水平（μｍｏｌ／Ｌ）明显高于同孕周健康孕妇的血浆Ｈｃｙ水平 （７．５６ ± ２．９７ ｖｓ． ５．９６ ± ２．５４, Ｐ ＝ ０．０１５）,而早产临产孕妇的血浆叶酸水平（μｍｏｌ／Ｌ）明显低于同孕周健康孕妇血浆叶酸水平（１６．２１ ± ２．９１ ｖｓ． １８．５３ ± ３．４４, Ｐ ＝ ０．００２）。早产临产孕妇ＭＴＨＦＲ的活性（ｎｍｏｌ·ｍｇ－１·ｈ－１）明显低于同孕周健康孕妇ＭＴＨＦＲ的活性（１３．３３ ± ３．１６ ｖｓ． ２６．８６ ± ５．６３, Ｐ ＝ ０．０００）。在ＭＴＨＦＲ基因中ｒｓ１８０１１３１和ｒｓ１８０１１３３在早产临产孕妇组与同孕周健康孕妇组的基因型分布差异有统计学意义（Ｐ ＜ ０．０５）。【结论】 早产儿孕妇血浆同型半胱氨酸水平受叶酸水平影响,并与ＭＴＨＦＲ基因ＳＮＰ分型密切相关。     

mthfr基因第1298位核苷酸多态性与酶活性的关系

目的：探讨ｍｔｈｆｒ基因第１２９８位核苷酸的多态性对酶活性及热稳定性的影响。方法用ＰＣＲ－ＲＦＬＰ方法检测６４全名国女性ｍｔｈｆｒ基因第１２９８位核 到，并检测中血淋巴细胞ＭＴＨＦＲ酶活性和４６℃灭活５ｍｉｎ后的歼余酶活性，比较不同基因型其酶活性和热稳定性的差异。结果：第６７７位核苷酸基因型为６７７ＣＣ时，正常型１２９８ＡＡ平均酶活性最高，为每毫克蛋白每小时１２．２６３ｎｍｏｌＣＨ２Ｏ，杂合型１２     

聚合酶链反应技术在腓骨肌萎缩症基因诊断中的应用

目的 探讨应用聚合酶链反应（ＰＧＲ）技术建立中国人腓骨肌萎缩症（ＣＭＴ）基因诊断的方法。方法 分别应用ＰＣＲ－双酶切、聚合酶链反应－单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）、聚合酶链反应－变性梯度凝胶电泳（ＰＣＲ－ＤＧＧＥ）或ＰＣＲ直接测序等方法对３２个确诊的ＣＭＴ家系进行了ＰＭＰ２２、ＭＰＺ、Ｇx３２等致病基因的突变检测。结果 ２１．９％的ＣＭＴ家系患者有Ｇx３２、ＭＰＺ和ＰＭＰ２基因的突变。ＰＣＲ－ＳＳＣＰ检测到１０个家系有异常泳带,经ＰＣＲ测序证实有５个为多态;另５个为与疾病相关的致病的点突变,即４个Ｇx３２和１个ＭＰＺ基因的点突变。PCR-双酶切诊断了２个包含ＰＭＰ２２基因在内的大片段重复突变的ＣＭＴ１Ａ型家系。ＰＣＲ－ＤＧＧＥ仅１个家系患者异常泳带,该结果也可经ＰＣＲ－ＳＳＣＲ方法检出。结论 ＰＣＲ－ＳＳＣＰ和ＰＣＲ－双酶切可作为Ｇx３２、ＭＰＺ、ＰＭＰ２２基因的点突变和ＣＭＴ１Ａ的大片段重复突变的初筛的方法,而ＰＣＲ－ＤＧＧＥ方法用于Ｇx３２基因的突变检测不理想,点突变应经ＤＮＡ测序证实。     

情感性障碍与单胺氧化酶基因的遗传关联性分析

目的 探讨单胺氧化酶（ＭＡＯＡ）和ＭＡＯＢ型基因多态性与情感性障碍（双相型和单相型）间的遗传关联性。方法 应用聚合酶链反应（ＰＣＲ）扩增片段长度多态性（Ａｍｐ－ＦＬＰ）研究１３２例情感性障碍患者的ＭＡＯＡ（ＣＡ）Ｎ，ＭＡＯＢ（ＧＴ）ｎ和ＭＡＯＢ（ＴＧ）ｎ基因座的基因型分布。结果 在１３２例患者中观察到ＭＡＯＡ（ＣＡ）ｎ，ＭＡＯＢ（ＧＴ）ｎ和ＭＡＯＢ（ＴＧ）ｎ基因座的等位基因数目分别为８，１２和９个     

青年缺血性脑卒中与MTHFRC677T、CBS844ins68基因多态性的关系

目的：研究人MTHFR、CBS基因多态性与青年缺血性脑卒中的遗传关联性。方法：采用限制性内切酶片段长度多态性方法（PCR-RFLP）,对98例脑卒中患者（病例组）和116例健康人（对照组）MTHFR C677T及CBS844ins68多态性位点进行检测。结果：病例组MTHFR基因T、C等位基因频率分别为54.09%和45.91%,对照组为37.93%和62.07%,两组比较差异有显著性（P<0.05）。TT型携带者与CC型携带者罹患脑卒中比较相对危险度为2.83,T等位基因携带者与C等位基因携带者罹患脑卒中比较相对危险度为1.93。CBS844ins68多态性位点在病例组与对照组之间D/I 2种等位基因及基因型分布频率差异无显著性(P>0.05)。Logistic回归分析显示MTHFRC677T是青年缺血性脑卒中的一个独立危险因子,且吸烟及高血压也与之有关联(P<0.01)。结论：汉族人群MTHFR C677T位点多态性与青年缺血性脑卒中有相关性,MTHFR基因可能是青年缺血性脑卒中的一个易感基因。CBS844ins68多态性位点与青年缺血性脑卒中无关联。     

亚甲基四氢叶酸还原酶基因多态性与糖尿病合并冠心病的相关性

目的:研究亚甲基四氢叶酸还原酶(MTHFR)基因多态性及血浆同型半胱氨酸水平与2型糖尿病患者合并冠心病的关系。方法:运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP)检测228例2型糖尿病患者(其中126例伴冠心病)及114例正常对照组MTHFRC677T基因型,采用高效液相色谱法测定血浆同型半胱氨酸水平。结果:在114例正常对照组中突变T等位基因频率为38.0%,与中国香港地区人群T等位基因频率(33.0%)相似。糖尿病组MTHFR基因型频率分布与正常对照组相比差异无统计学意义(χ2=3.67,P>0.05)。糖尿病伴冠心病组MTHFRT等位基因频率(45.2%)明显高于糖尿病不伴冠心病组(30.4%),基因型和等位基因频率分布差异均有统计学意义(分别为χ2=11.98,P<0.01;χ2=8.72,P<0.01),T等位基因与糖尿病并发冠心病密切相关(OR=1.89,95%CI:1.24-2.88))。糖尿病伴冠心病组、糖尿病不伴冠心病组及正常对照组中,MTH-FR基因有C677T突变者血浆同型半胱氨酸水平均显著高于无基因突变者。结论:MTHFR基因C677T位碱基突变致血浆同型半胱氨酸水平升高可能是糖尿病并发冠心病的重要遗传因素。     

Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients

Objective Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus (T2DM). Previous researches report that methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms might influence the occurrence of coronary heart disease (CHD) in T2DM patients. The purpose of this study was to evaluate whether MTHFR C677T and A1298C mutations are associated with the risk of CHD in T2DM patients. Methods A total of 197 subjects with T2DM were studied, of which 95 patients with CHD. The genotypes of MTHFR C677T and A1298C were analyzed by using dideoxy chain-termination method, and compared between patients with CHD and those without CHD. Results We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD (P=0.011). However, there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD. Furthermore, the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine (Hcy) levels (≤15 μmol/L) (P=0.006), while no effect of MTHFR gene polymorphism on the incidence of CHD was found in patients with higher Hcy levels (>15 μmol/L) (P=0.491). Conclusion The MTHFR C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hcy levels.     

河南汉族冠心病患者N5,N10-亚甲基四氢叶酸还原酶基因C677T多态性检测

目的:探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与冠心病(CHD)的相关性.方法:应用PCR-限制性片断长度多态性(RFLP)技术,检测208例CHD患者和500名健康人(正常组)MTHFR C677T基因多态.结果:MTHFR基因第677位存在2种等位基因(C和T)、3种基因型(C/C、C/T、T/T).CHD组C/C、C/T、T/T 3种基因型频率分别为15.4%、45.7%、38.9%,T等位基因频率为61.8%,C等位基因频率为38.2%;正常组C/C、C/T、T/T型频率分别为35.6%、44.0%、20.4%,T等位基因频率为42.4%,C等位基因频率为57.6%.CHD组T/T型及T等位基因频率均高于对照组(P＜0.05).结论:MTHFR基因C677T多态性可能与河南汉族人群CHD的发生有关.     

RELATIONSHIP OF HOMOCYSTEINE AND GENE POLYMORPHISMS OF ITS RELATED METABOLIC ENZYMES WITH ALZHEIMER＇S DISEASE

OBJECTIVE: To investigate the relationship of plasma homocysteine (Hcy) levels and the gene polymorphisms of N5, N10-methylenetetrahydrofolate reductase (MTHFR), cystathionine beta-synthase (CBS) with Alzheimer's disease (AD). METHODS: Plasma Hcy levels were measured by means of high voltage capillary electrophoresis with ultra-violet detection, the polymorphisms of C677T in exon 4 of MTHFR gene and 844ins68 in exon 8 of CBS gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 105 AD patients and 102 non-AD controls. All controls were excluded from cardiocerebrovascular disorders and other diseases. RESULTS: The plasma Hcy level in AD patients (16.04 +/- 3.84 micromol/L) was significantly higher than that in the controls (11.94 +/- 3.87 micromol/L, P < 0.001). There were no significant differences of the genotype and allele frequencies of MTHFR C677T mutation and CBS 844ins68 mutation between the patients and controls. However, the T allele of MTHFR gene was found to relate with the plasma Hcy level increase in all subjects. CONCLUSION: The elevated plasma Hcy level in AD patients is probably involved in the pathogenesis of AD, which may be due to the environmental factor rather than genetic factors of the mutations of MTHFR and CBS.     

MTHFR基因多态性与脑卒中的关系

目的 :研究人体 N5 ,N1 0亚甲基四氢叶酸还原酶 ( MTHFR)的基因多态性与脑卒中的遗传相关性。方法 :采用限制性内切酶片段长度多态性方法 ( PCR- RFLP) ,对 1 1 8例脑卒中患者和1 39例健康人 MTHFR基因 C677T多态性位点进行检测。结果 :病例组 MTHFR基因 T等位基因频率为 5 0 .4%,C等位基因频率为 49.6%;对照组 T等位基因频率为 40 .6%,C等位基因频率为5 9.4%;差异有显著性 ( P<0 .0 5 )。出血性卒中 T等位基因频率为 41 .4%,C等位基因频率为5 8.6%;缺血性卒中 T等位基因频率为 5 4 .2 %,C等位基因频率为 45 .8%;无明显差异。结论 :脑卒中汉族人群 MTHFR基因 C677T突变等位基因频率与健康人群存在差异 ;出血性脑卒中患者与缺血性脑卒中患者之间 ,基因型及等位基因频率均无明显差异。表明 MTHFR基因 C677T突变位点多态性与脑卒中有相关性 ,MTHFR基因可能是脑卒中的一个易感基因     

The 677 C/T MTHFR polymorphism is associated with essential hypertension, coronary artery disease, and higher homocysteine levels

BACKGROUND: Essential hypertension (EH) and cardiovascular disease are common, multifactorial disorders likely to be influenced by multiple genes of modest effect. The C677T methylenetetrahydrofolate reductase (MTHFR) gene polymorphism is related to MTHFR enzyme activity and to plasma homocysteine (Hcy) concentration. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD), EH, and healthy subjects. METHODS: In this study, we measured serum folate, serum vitamin B12, and plasma homocysteine and determined the MTHFR C677T genotype of 78 patients with essential hypertension, 100 patients with coronary artery disease, and 100 healthy subjects. MTHFR genotypes were assessed by real-time polymerase chain reaction. RESULTS: CC, CT, and TT genotype frequencies were 52, 44.0, and 4.0% in patients with CAD, respectively. In patients with essential hypertension, the CC, CT, and TT genotype frequencies were 46.2, 41.0, and 12.8%, respectively. In control subjects, the CC, CT, and TT genotype frequencies were 72.0, 26.0, and 2.0%, respectively. The C allele was significantly more frequent in controls compared with patients with EH (p<0.05), and CC genotypes were more frequent in controls compared to patients with EH and CAD. Homocysteine level was higher in TT genotypes in CAD patients compared with CC and CT genotypes (p<0.01). MTHFR gene polymorphism is an independent risk factor for EH but not for CAD. CONCLUSIONS: The TT genotype of the 677C/T MTHFR polymorphism is associated with EH and CAD. In addition, TT genotypes had higher plasma Hcy levels in CAD patients compared with CC and CT genotypes. MTHFR gene polymorphism is an independent risk factor for EH but not for CAD.     

亚甲基四氢叶酸还原酶基因多态性与抑郁症的关联研究

目的 探讨亚甲基四氢叶酸还原酶(MTHFR)C677T多态性与抑郁症及症状表型的相关性.方法采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)研究100例抑郁症患者及100例对照组正常人的MTHFR基因的多态性分布;采用汉密尔顿抑郁量表(HAMD)评定症状表型.结果抑郁症患者组TT型、TC型、CC型基因频率分布及T、C等位基因频率分布与对照组比较差异有显著性(χ2=8.72,P<0.05;χ2=8.62,P<0.05).按性别分层后,男性抑郁症组TT基因型的频率(25.0%)明显高于对照组(6.3%),而CC基因型的频率(25.0%)显著低于对照组(55.3%)(P<0.05);患者组中症状表型在3种基因型间分布差异无显著性(P＞0.05).结论 MTHF C677T基因的多态性与抑郁症发病相关,其相关性受性别影响,而与症状表型间无明显的关联.     

RELATIONSHIP OF HOMOCYSTEINE AND GENE POLYMORPHISMS OF ITS RELATED METABOLIC ENZYMES WITH ALZHEIMER''''S DISEASE

HOMOCYSTEINE (Hcy), one of the thiol-contain-ing amino acids, is generated from the demethyla-tion of methionine and in very low level in thehuman body. A lot of studies indicate that elevated plasmaHcy level has been recognized as an independent risk factorfor the atherosclerotic events.1 Recently, many attentionshave been paid to the association of Hcy with the dev-elopment of Alzheimer's disease (AD) and other neuro-degeneration disorders.2 Therefore, in this present study weinvestig…