创伤后巨噬细胞对T淋巴细胞的直接接触免疫抑制作用

本实验研究了创伤小鼠巨噬细胞对Ｔ淋巴细胞的直接接触抑制作用。结果显示：创伤后巨噬细胞可通过直接的细胞接触方式抑制正常小鼠Ｔ淋巴细胞转化、ＩＬ－２的产生、ＩＬ－２４的表达以及ＩＬ－２介导的淋巴细胞增殖，但对ＩＬ－２－ＩＬ－２Ｒ反应及ＩＬ－１的产生无明显影响，可明显提高Ｔｓ细胞抑制活性。去除正常小鼠淋巴细胞中的Ｔｓ细胞，可阻断创伤后巨噬细胞的这一抑制活性。提示：（１）创伤后巨噬细胞可通过ＩＬ－１以外的     

电针对创伤大鼠白介素－1和β－内啡肽影响的时效观察

动态观察电针穴位对创伤大鼠神经内分泌免疫功能的影响。用手术创伤大鼠的模型，电针刺激＂足三里＂、＂阑尾穴＂，经３Ｈ－胸腺嘧啶核苷孵育分析，测定巨噬细胞分泌白介素－１（ＩＬ─１）的功能及外围血β─内啡呔和皮质酮水平。结果：创伤组２、４、８、１２ｈ大鼠胸腔巨噬细胞（ＭΦ）分泌ＩＬ─１的功能明显增强（Ｐ＜０．０５，Ｐ＜０．００１）。各时期的血浆β─内啡肽（β─Ｅｎｄ）和皮质酮（ｃｓ）含量极明显升高（Ｐ＜０．０５；Ｐ＜０．００１），创伤大鼠经电针后，１、２、４ｈ，ＩＬ─１显著升高（Ｐ＜０．０５），８ｈ与正常大鼠巨噬细胞分泌ＩＬ─１的功能无区别，较创伤组大鼠提早４ｈ恢复正常功能，β─Ｅｎｄ含量各时相均明显低于创伤组大鼠水平（Ｐ＜０．０５；Ｐ＜０．００１）。皮质酮的含量１、２、４ｈ明显低于创伤组大鼠水平（Ｐ＜０．０５；Ｐ＜０．００１）。结论：电针穴位对手术创伤引起的机体应激状态具有良性调节作用。     

创伤小鼠白细胞介素－6的变化

采用小鼠烧伤、截肢伤、闭合伤模型，观察创伤后血清中白细胞介素－６（Ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）的变化，并测定其巨噬细胞，淋巴细胞ＩＬ－６的生成情况。结果显示：３种创伤情况下，血清中ＩＬ－６水平均升高，以烧伤小鼠最明显。创伤后巨噬细胞及不同来源淋巴细胞（脾细胞、胸腺细胞、肠系膜淋巴结细胞）自发产生ＩＬ－６及在ＬＰＳ或ＣｏｎＡ刺激下产生ＩＬ－６的能力均明显增强。     

复发性口疮患者免疫功能的研究

采用速率散射比浊法、淋巴细胞转化实验、巨噬细胞吞噬实验及间接免疫荧光法对复发性口疮（简称ＲＡＵ）患者免疫功能进行了检测。结果表明：（１）ＲＡＵ患者血清ＩｇＧ、ＩｇＡ、Ｃ４含量高于正常人（Ｐ＞０．０５）。（２）淋巴细胞转化率、巨噬细胞吞噬率均低于正常人（Ｐ＜０．０５）。Ｔ淋巴细胞总数（Ｔ３）低于正常人，抑制性Ｔ细胞（Ｔ８）高于正常人，辅助性Ｔ细胞（Ｔ４）与Ｔ８比值下降（Ｐ＜０．０５）。说明ＲＡＵ患者体液免疫功能增强，细胞免疫水平处于抑制状态。提示ＲＡＵ的发病机理与自身免疫功能的紊乱有关     

创伤小鼠白细胞介素—6的变化

采用小鼠烧伤／截肢伤，闭合伤模型，观察创伤后出血清中白细胞介素－６（Ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）的变化，并测定其巨噬细胞，淋巴细胞ＩＬ－６的生成情况。结果显示：３种创伤情况下，血清中ＩＬ－６水平均升高，以烧伤小鼠最明显。创伤后巨噬细胞及不同来源淋巴细胞（脾细胞，胸腺细胞，肠系膜淋巴结细胞）自发产生ＩＬ－６及在ＬＰＳ或ＣｏｎＡ刺激下产生ＩＬ－６的能力均明显增强。     

心肌挫伤后心肌酶谱和心脏肌钙蛋白-T的含量变化

目的：观察心脏肌钙蛋白－Ｔ（ｃＴｎ－Ｔ）和心肌酶谱（ＭＥ）在心肌挫伤（ＣＣ）后含量变化及其对ＣＣ的诊断价值。方法：采用兔钝性胸部创伤（ＢＣＴ）模型，致成中度至重度ＣＣ，分别在伤前、伤后４、８和２４ｈ抽箅测定血清ｃＴｎ－Ｔ和ＭＥ含量。结果：伤后４ｈ，血精ｃＴｎ－Ｔ和ＭＥ含量明显升高（Ｐ〈０．０１），持续至伤后２４ｈ。其中ｃＴｎ－Ｔ在伤后２４ｈ升高最为明显，ＭＥ有下降，但仍然明显高于伤前（Ｐ〈０．０１     

流行性出血热灭活疫苗人体试验的免疫反应观察

由流行性出血热病毒浙１０株感染沙鼠肾细胞，经０．０５％β－丙内酯灭活研制成的流行性出血热灭活疫苗，对８名志愿者免疫３针（０、７、２８天各注射１ｍｌ）。首针免疫后第２８天，血清用ＲＰＨＩＡ，ＥＬＩＳＡ和ＩＦＡ检测流行性出血热抗体，均全部阳转，空斑减少中和试验（ＰＲＮＴ）于第２８天有４人阳转，第４５天又有３人阳转，阳转率８７．５％，ＧＭＴ４０．０。于志愿免疫前及后７、１４、２８、４５天、３个月、０．５年、１年测定其３ＨＴｄＲ掺入法淋转试验显示：首针免疫后７天至３个月以内，其非特异性和（或）特异性Ｔ淋巴细胞转化程度低下，与免疫前相比有显著差异（Ｐ＜０．０５～０．００１）；特异性Ｂ淋巴细胞转化于免疫后１～１．５月明显增高，与免疫前相比Ｐ＜０．０５；非特异性的Ｔ、Ｂ淋巴细胞总的转化率亦以免后１～１．５月为高（Ｐ＜０．０５）。１年后，再加强免疫１针。加强免疫后１月测中和抗体、阳转率１００％、ＧＭＴ１１７．４，比初免高２倍，两者差异极为显著，特异与非特异性Ｔ淋巴细胞转化未再见抑制（Ｐ＞０．０５），而特异性Ｂ淋巴细胞转化极为明显增强（Ｐ＜０．００１）。     

肿瘤坏死因子—α,转化生长因子—β1,血小板源性生长因子,？…

目的 研究支架术后不同时间局部食管组织中相关细胞因子的表达情况,以及与再狭窄形成的关系。方法 采用免疫组化（ＡＢＣ法）检测支架术后１、２、４、８周组织中肿瘤坏死因子－α（ＴＮＦ－α）,转化生长因子－β１（ＴＧＦβ１）、血小析 性生长因子（ＰＤＧＦ）、内皮素１（ＥＴ１）的表达及其变化规律。结果 支架术后１周组织即开始表达ＴＮＦ－α,术后２周ＴＮＦ－α,ＴＧＦβ１、ＰＤＧＦ、ＥＴ１表达均明显增强,术后     

创伤小鼠淋巴细胞内环磷酸腺苷,环磷酸鸟苷的变化

用小鼠闭合伤、截肢伤及烧伤模型，观察创伤后４ｄ小鼠脾细胞、胸腺细胞、肠系膜淋巴结细胞内环磷酸腺苷（ｃＡＭＰ）、环磷酸鸟苷（ｃＧＭＰ）的变化。结果显示：创伤后三种类型的淋巴细胞在静息状态或经ＣｏｎＡ刺激时的ｃＡＭＰ水平增高，ｃＧＭＰ水平下降，ｃＡＭＰ／ｃＧＭＰ比值增加。创伤后血清、巨噬细胞、Ｔｓ细胞在体外可明显提高正常小鼠脾细胞内ｃＡＭＰ水平，降低其ｃＧＭＰ水平。去除创伤小鼠脾细胞中的巨噬细胞、Ｔｓ     

内毒素对呼吸道上皮分泌内皮素,血栓烷素,前列腺素E2的影响及 …

目的 探讨内毒素对培养小牛呼吸道上分泌内皮纱－１（ＥＴ－１）血栓烷素（ＴＸＡ２）前列腺素Ｅ２（ＰＧＥ２）的影响，并探讨氧自由基的介导作用。方法 大肠杆菌内毒素（ＬＰＳ）与培养小牛呼吸道上皮细胞孵育４小时后，测定培养呼吸道上的细胞上清液中，ＥＴ１，ＴＸＡ２ＰＧＥ２的含量。结果 内毒素可明显促进小牛呼吸道上皮细胞ＥＴ－１，ＴＸＡ２的分泌，抑制ＰＧＥ２分泌，并呈浓度依赖性，用氧自由基清除剂ＳＯＤ，Ｃａｔ     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…