植物蛋白MAP30等药物抗HBV的体外实验

目的 :体外观察植物蛋白MAP30 (Momordicaanti HIVproteinof 30ku)、α 干扰素 (α IFN)、无环鸟苷 (ACV)及叠氮胸苷 (AZT)的抗HBV作用 .方法 :以HepG2 .2 .15细胞为靶细胞 ,实验共进行 9d .采用ELISA法及荧光定量PCR法 ,测定药物对细胞培养上清HBsAg ,HBeAg及细胞内外HBVDNA的抑制作用 .结果 :MAP30具有明显抗HBV的作用 ,其抑制HBsAg,HBeAg及细胞内外HBVDNA的 5 0 %抑制浓度 (IC50 )分别为 0 .8,0 .6 ,0 .6及 0 .5 μmol·L-1,治疗指数 (TI) >4～ 7.α IFN也有抑制HBV的作用 ,但抑制作用较弱 ,而ACV及AZT对HBV无抑制作用 .结论 :抗HIV植物蛋白MAP30具有体外抑制HBV的作用     

干扰素α对人肺腺癌细胞系A549增殖抑制和诱导分化作用

目的 :探讨干扰素α(IFN α)对人肺腺癌细胞的增殖抑制和诱导分化作用。方法 :用不同质量浓度IFN α(2 5 μg/L ,75 μg/L ,10 0 μg/L)处理人肺腺癌A54 9细胞 ,记录细胞数目 ,进行甲基绿 派洛宁染色 ,测定软琼脂集落形成率。结果 :IFN α能明显抑制A54 9细胞的增殖 ,药物组与对照组之间差异有显著性 (P <0 0 5 ) ,药物组与对照组比较增殖型细胞减少 ,分化型细胞数增加 ,双层软琼脂中细胞集落形成受到明显抑制 ,对照组集落普遍比药物组集落大 ,且单个集落中细胞数目多 (P <0 0 5 )。结论 :IFN α对A54 9细胞具有增殖抑制和诱导分化作用     

异丙酚对人脐静脉内皮细胞凋亡相关基因Bcl-2及Bax表达的影响

目的 :观察异丙酚 (Propofol)及肿瘤坏死因子 α(TNF α)对培养的人脐静脉内皮细胞 (HUVECs)凋亡相关基因Bcl 2及Bax表达的影响 ,探讨异丙酚抑制凋亡的机理。方法 :将培养的人脐静脉内皮细胞 3～ 4代于融合状态 ,随机分为 7组 :对照组 (P0 ) ,2 5 μmol/L异丙酚组 (P2 5) ,TNF α组 (P0 +TNF α) ,12 .5 μmol/L异丙酚和TNF α组 (P12 .5+TNF α) ,2 5 μmol/L异丙酚和TNF α组 (P2 5+TNF α) ,5 0 μmol/L异丙酚和TNF α组 (P50 +TNF α) ,10 0 μmol/L异丙酚和TNF α组 (P10 0 +TNF α)。各组加入相应药物培养 2 4h后收获细胞 ,采用免疫细胞化学染色方法测定Bcl 2及Bax蛋白表达。结果 :与对照组 (P0 )相比 ,异丙酚 2 5 μmol/L组 (P2 5)Bcl 2及Bax蛋白表达无明显变化 (P >0 .0 5 ) ;TNF α(P0 +TNF α)组Bcl 2蛋白表达降低 ,Bax蛋白表达升高 (P <0 .0 0 1) ;而不同浓度的异丙酚预处理后再加入肿瘤坏死因子的各组 (P12 .5+TNF α、P2 5+TNF α、P50 +TNF α、P10 0 +TNF α)Bcl 2蛋白表达增加 ,Bax蛋白表达降低 (P <0 .0 5或P <0 .0 1)。结论 :临床相关浓度的异丙酚可通过调节Bcl 2及Bax蛋白表达抑制TNF α诱导的内皮细胞凋亡     

虎杖黄芪合用抗HSV_(-2)药效分析

目的 :为了得到新的抗HSV中药方剂的最佳配方比 ,我们进行了此项研究。方法 :我们以阿昔洛韦 (ACV)为阳性对照 ,采用空斑减数实验 ,观察了虎杖、黄芪单用及合用抗HSV- 2 333株的药效 ,用中效作用原理对药效进行了分析。结果 :在HEp 2 细胞系统中 ,ACV对HSV- 2 333株直接杀灭、增殖抑制、感染阻断的ED50 依次为 16 .4 5、10 .85、18.6 2 μg/ml,虎杖黄芪合用比为 1(Dm )虎杖 / 1(Dm )黄芪时 ,相应数值分别为 2 .2 7、2 .0 4、1.32 μg/ml。与ACV相比 ,药效比ACV强 ,而且合用指数 (CI)平均值最小 ,协同作用最明显。结论 :上述合用比对HSV- 2 333株直接杀灭、增殖抑制、感染阻断的治疗指数 (TI )是ACV的 72 6、52 5、140 0倍 ,是组方时的最佳配比。     

PKC-α反义核酸对宫颈癌HeLa细胞增殖及细胞周期的影响

目的 :探讨 PKC-α反义核酸对 He L a细胞增殖及细胞周期的影响。方法 :采用脂质体 (L P)介导法 ,用 0 .0 1～1.0 0μmol/L各浓度 PKC-α反义核酸 (as ODN)转染 He L a细胞 ;MTT法及软琼脂克隆形成法分别检测 He L a细胞生长指数 (growth index,GI)及其体外增殖能力 ;流式细胞术分析细胞周期。结果 :PKC- α as ODN0 .0 5～ 1.0 0 μmol/L 各浓度组 He L a细胞 GI显著降低 (P<0 .0 5 ) ,且呈量效依赖关系 ;其中 1.0 0μmol/L和 0 .5 0μm ol/L PKC-α as ODN对He L a细胞生长有非常显著抑制作用 (P<0 .0 1) ;0 .5 0 μmol/L PKC- α as ODN组 He L a细胞软琼脂克隆形成率均低于对照组 (P<0 .0 5 ) ;反义 PKC- α使细胞 G2 期百分比增高 (P<0 .0 1)。结论 :反义 PKC- α可通过阻滞细胞于 G2 期从而抑制 He L a细胞的生长。     

α干扰素对A_(549)细胞端粒酶活性和p53蛋白的表达

目的 :探讨α 干扰素 (IFN α)对人肺腺癌细胞株A549细胞端粒酶活性和p5 3蛋白质表达的影响。方法 :用两种不同质量浓度IFN α(2 5 μg L ,10 0 μg L)处理人肺腺癌细胞株A549细胞 ,并设空白对照。采用TRAP 银染法检测端粒酶活性 ,用免疫荧光标记法测定p5 3蛋白的表达。结果 :IFN α作用后的A549细胞 ,端粒酶活性明显降低 ,且p5 3蛋白表达明显减少。结论 :IFN α能抑制肺腺癌细胞的端粒酶活性和p5 3蛋白的表达     

PI3K/Akt信号与吴茱萸碱抑制人结肠癌细胞增殖的关系研究

目的 研究吴茱萸碱(Evodiamine,Evo)对结肠癌LoVo细胞增殖的抑制作用及可能的分子机制.方法 采用结晶紫染色分析Evo(0、0.5、1、2、4μmol/L)对LoVo细胞的增殖抑制作用;应用流式细胞术分析Evo(0、0.5、1、2μmol/L)对LoVo细胞凋亡的促进作用;Western blot法分析Evo(0、0.5、1 μmol/L)对LoVo细胞中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)及Caspase-3蛋白表达水平的影响;利用缺氧诱导因子-1 α(hypoxia inducible factor 1-alpha,HIF-1 α)荧光素酶报告质粒检测Evo(0、0.5、1、2μmol/L)对HIF-1 α转录活性的影响.采用Western blot法分析Evo(0、0.5、1、2μmol/L)对LoVo细胞中HIF-1 α、Akt1/2和磷酸化Akt1/2蛋白水平的影响.结果 与对照组相比,Evo明显抑制LoVo细胞增殖,下调PCNA蛋白水平,促进LoVo细胞凋亡;报告质粒统计结果显示,Evo呈浓度依赖性降低HIF-1α荧光素酶报告质粒活性(Evo为0.5μmol/L时,P＜0.05;Evo为1μmol/L或2μmol/L时,P＜0.01);Western blot检测结果显示,Evo能够明显降低HIF-1 α蛋白水平,下调Akt1/2磷酸化水平.结论 Evo能够抑制LoVo细胞增殖并促进凋亡,其机制可能与Evo下调HIF-1α蛋白表达,抑制PI3 K/Akt信号转导相关.     

MAP30 抗乙型肝炎病毒的体外实验

目的 :探讨MAP30 (momordicaanti HIV proteinof30ku)体外抗乙型肝炎病毒 (HBV)的作用 .方法 :以HBVDNA转染的人肝癌细胞株 (HepG2 .2 .15 )为靶细胞 ,与MAP30共同温育 10d ,观察对细胞的毒性 ;用ELISA、荧光定量PCR和Southern印迹法分别检测培养上清中HBsAg ,HBVDNA和细胞内HBVDNA .结果 :MAP30能显著减少HepG2 .2 .15细胞培养上清液中HBsAg ,HBVDNA的分泌 ,在第 8日时 ,对HBsAg抑制率为 5 0 .4 9% ,HBVDNA定量 <1× 10 7拷贝·mL-1;Southern印迹法显示MAP30能抑制HBV复制中间体及共价环状闭合DNA .MAP30未发现对细胞有毒性作用 .结论 :MAP30具有较强的体外抗HBV作用 ,作为新的抗病毒植物蛋白 ,具有其临床应用价值     

喷昔洛韦体内抗单纯疱疹病毒的作用

目的 :研究喷昔洛韦 (PCV)对单纯疱疹病毒 (HSV)感染小鼠的疗效。方法 :每只分别用病毒滴度为 10 0LD50 ·(0 .1ml) - 1 HSV 1、HSV 2腹腔注射 0 .0 3ml,2 4h后皮下注射给药 ,PCV剂量分别为 2 0 ,5 0 ,10 0 ,2 5 0mg·kg- 1 ·d- 1 ,疗程 5d。观察动物的反应 ,出现死亡时间和死亡数 ,所有动物用药后观察 15d。同时收集各组动物治疗后的标本 ,进行病毒分离 ,按常规测定病毒滴度。另设病毒对照组、0 .9%NaCI对照组、阳性药阿昔洛韦对照组 (ACV)。结果 :PCV对HSV感染鼠有保护作用 ,5 0mg·kg- 1 ·d- 1 时 ,HSV 1和HSV 2感染鼠的存活率分别为 6 0 %和 75 % ,平均存活时间 (d)分别为 11.3± 3.6和 12 .9± 2 .8,随着药物剂量增加 ,疗效增强 ,该药还能降低组织内病毒滴度 ,阻止体内病毒复制 ,抗HSV作用类似于相同剂量的ACV。结论 :PCV有抗HSV作用。     

穿琥宁粉针剂体外抗HIV-1作用

目的: 探讨穿琥宁粉针剂在体外是否具有抗HIV作用. 方法:采用荧光染料Calcien AM标记HIV感染的慢性H9细胞(H9/HIV-1ⅢB)与不同稀释浓度的穿琥宁药物作用后,与靶细胞MT-2细胞混合培养,观察MT-2和H9/HIV-1ⅢB融合细胞的变化;MTT法检测穿琥宁对HIV感染细胞的保护作用;HIV-1P24抗原试剂盒检测药物作用后的细胞培养上清P24抗原含量的变化. 结果:穿琥宁粉针剂能够抑制MT-2和H9/HIV-1ⅢB细胞融合(其抑制融合的EC50为5.49 mg/L,选择指数SI为327.6);能够保护HIV感染细胞的死亡(EC50为64.57 mg/L,SI为27.85)和减少HIV-1 P24抗原的表达(EC50为194.67 mg/L,SI为9.23). 结论:穿琥宁粉针剂在体外具有抗HIV-1的作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.