广州地区西藏小型猪的繁殖行为学表现

目的对广州地区西藏小型猪的繁殖性能和繁殖行为进行了观察研究。方法以广州地区雌雄西藏小型猪为研究对象,根据其繁殖卡的详细资料进行分类统计。结果雄性西藏小型猪阴茎首次伸出日龄为(39.50±2.63)d,此时体重为(3.26±0.87)kg;其射精日龄为(65.22±3.69)d,体重为(5.34±1.03)kg。雌性西藏小型猪初情日龄为(142±5.3)d,成年母猪发情周期为(20.35±0.72)d,发情持续期(4.2±0.31)d;其妊娠期约为114 d左右。初产雌性西藏小型猪产仔数为(2.30±0.82)头,2月龄断乳存活率为86.5%;而经产雌性西藏小型猪产仔数为(5.70±1.00)头,其2月龄断乳存活率为91.4%。哺乳期雌性西藏小型猪母性强,护仔行为强烈。结论广州地区西藏小型猪生长发育繁殖正常,产仔率和2月龄存活率等繁殖性能指标与原产地一致。     

巴马小型猪的选育

巴马小型猪的选育是以广西巴马香猪作为原始材料。进行适于实验应用的小型猪品系繁育。选育的主要性状是小体型。既要控制其体型不增大,又要使其尽量微型化,同时兼顾繁殖性能。配合饲料粗蛋白质含量为14.77%。妊娠、哺乳母猪日给料量为其体重的2～3%。经三年三世代小群闭锁繁育,0～2世代群体有效含量分别为6.93、13.44、11.42。二世代平均近交系数为9.18%,平均亲缘系数为24.93%。0～2世代6月龄后备母猪平均体重分别     

西藏小型猪F1代与F0代生长繁殖性能的比较

目的比较研究广州地区西藏小型猪F1代与F0代引入群母猪繁殖和仔猪生长性能的差异。方法观察记录西藏小型猪F1代和F0代的产仔数、产活仔数、离乳仔数、初生重、30日龄和60日龄体重。结果西藏小型猪F1代和F0代母猪其初产平均产仔数、产活仔数、离乳仔数、平均初生重、30日龄和60日龄体重分别为3.83头、2.30头;3.29头、2.20头;3.14头、3.11头;0.66 kg、0.53 kg;2.70 kg2、.30 kg;4.50 kg、4.01 kg;F1代初产仔数和离乳率均显著高于F0代。结论西藏小型猪已经适应了广州高温高湿环境,能够稳定地生长繁殖。     

融水小型猪F1代生长研究

目的 测定融水小型猪F1代体重和体尺。方法 选取F1代融水小型猪83头（雌性48头,雄性35头）,测定初生至12月龄的体重、体长、体高、胸围、胸宽、胸深、管围、腿围、嘴裂长度共9个生长发育指标,并应用SPSS统计软件和Logistic非线性生长模型进行分析。结果 融水小型猪F1代的初生体重雌雄分别为0.61±0.14 Kg和0.55±0.13 Kg,6月龄体重雌雄分别为17.21±5.20 Kg和16.35±5.23 Kg,12月龄体重雌雄分别为26.97±6.49 Kg和26.53±5.65 Kg。雌雄比较,9项指标所测结果接近,除了初生体重和体长、10月龄胸宽有差异（P<0.05）,其余指标同月龄雌雄之间均无明显差异。应用Logistic模型分析,体重生长拐点在5～6月龄间,体长和腿围生长拐点在2～3月龄间,体高、胸围、胸宽、胸深、管围和嘴裂长度的生长拐点在1～2月龄间。结论 融水小型猪F1代成年体重轻,性情温顺,具备培养成实验用小型猪基本条件。     

融水小型猪的实验动物化系列研究概述

融水小型猪的种源来自广西融水县,2012年被引到广东繁育并测定了基础数据,包括种质特性、繁殖性能、生长曲线、血液学指标、血生化指标、脏器系数、染色体分析。参照国家和地方有关实验动物标准,初步建立了融水小型猪微生物与寄生虫、环境与设施、饲料、病理学、遗传学等质量控制标准。融水小型猪能适应当地气候,繁殖良好,自然受孕率为88.3%,妊娠期平均112 d,初产平均胎产仔6.1头,经产平均产仔7.9头。融水小型猪体型小,雌雄成年体重(6月龄)分别为17.21±5.20 kg和16.35±5.23 kg,性情温顺,线粒体DNA分析表明融水小型猪比兰屿猪更为古老。通过标准化的饲养管理与质量控制,具有培育成实验用小型猪的基本条件。     

合肥市区婴儿生长发育指标的调查分析

目的:了解合肥市活产新生儿出生时体格发育状况及婴儿阶段体格发育水平。方法:选取合肥市2007~2008年分娩新生儿进行婴儿期体格发育检测。新生儿出生时体格测量指标采用出生身长、出生体重、出生头围等。婴儿期体格发育监测资料包括3个月、6个月、9个月和12个月分别进行身长、体重、头围等体格发育指标的测量。结果:合肥市2007~2008年活产新生儿平均出生体重(3 370±451)g、出生身长(50.26±1.67)cm,出生头围(34.09±1.37)cm,出生后3个月、6个月、9个月和12个月的平均体重分别为(6.751±0.798)kg、(8.469±1.002)kg、(9.518±1.123)kg和(10.275±1.163)kg,平均身长分别为(62.18±2.20)cm、(68.54±2.36)cm、(73.02±2.53)cm和(76.77±2.57)cm,各月龄的男童体重、身长、头围水平均高于女童,且无论男童还是女童,6个月前(特别是3个月前)各体格指标增长水平明显高于6个月以后。合肥市市区婴儿各月龄组身高、体重均高于WHO推荐标准(2006版,下同);而体重男婴和女婴各有2个月龄高于全国9城市标准(2009版,下同),身高男婴有1个、女婴有2个月龄组高于全国9城市标准,头围符合全国9城市标准。结论:合肥市婴儿出生时和婴儿期内的体格发育总体上处于较好水平。WHO推荐的身高和体重标准已难以适应现阶段合肥市婴儿生长发育的评价。而全国9城市标准可以作为合肥市市区婴儿生长发育的评价标准。     

魔芋片治疗肥胖症疗效的初步观察(摘要)

作者用魔芋片治疗成年人单纯性肥胖者35例,男性4例,女性31例。平均体重74.55kg,平均体重指数49.6。服药前、后均在固定条件下检测血脂、体重及皮下脂肪厚度(下称皮脂厚度)。每例每次口服魔芋片2.4g,一日3次,连服2月,其中15例连服3个月。结果表明;35例治疗前和治疗第1、2两个月后,体重平均值分别为74.55±9.33kg和71.90±8.88kg、71.25±9.27kg。皮脂厚度平均值分别为3.32±0.54cm和3.11±0.46cm、3.01±0.45cm,表明体重和皮脂厚度均较治疗前有显著性差异     

巴马小型猪的选育

5世代选育结果，0-4世代群体有效含量6.93,13.44,11.42,7.45,10.76,2-4世代群体近交系数9.18%,22.50%,35.22%，4世代3-7月龄体重9.60,12.47,16.40,20.26,35.54kg,4世代初产仔数8.64头，初生重0.55kg，3世代二产10.60头，0.56kg。巴马小型猪近交程度较高，毛色理想并趋于整齐，矮小体型及多产等，主要特性遗传性稳定，已达选育目标。     

学龄前儿童微量元素及营养的合理摄入对生长发育的影响

目的：分析微量元素及营养的合理摄入对学龄前儿童生长发育的影响。方法：150例学龄前儿童,根据主食摄入量及食欲情况分为过量组25例、良好组59例、中等组47例、低下组19例,比较各组儿童蛋白质及热能摄入量,入组时及12个月后各组儿童身高与体重和血微量元素浓度,分析营养及微量元素与身高、体重的相关性。结果：过量组儿童日均蛋白质及热能摄入量大于其他三组,差异均有统计学意义（P＜0.05）,良好组日均蛋白质及热能摄入量大于中等组和低下组,差异均有统计学意义（P＜0.05）。12个月后良好组身高115.32±4.56 cm,高于过量组的113.49±4.39 cm,中等组的112.18±4.38 cm和低下组的110.53±4.87 cm,差异均有统计学意义（P＜0.05）,而过量组与中等组身高的差异无统计学意义（P＞0.05）,但均高于低下组,差异均有统计学意义（P＜0.05）。12个月后过量组体重25.59±3.32 kg,高于良好组的21.49±2.95 kg,中等组的18.94±3.12 kg和低下组的17.69±2.76 kg,差异均有统计学意义（P＜0.05）,良好组与中等组体重的差异无统计学意义（P＞0.05）,但高于低下组,差异均有统计学意义（P＜0.05）。12个月后中等组血钙、锌水平低于过量组及良好组,低下组各微量元素浓度低于其他组,差异均有统计学意义（P＜0.05）。儿童身高、体重与营养及微量元素的摄入量呈正相关,相关系数r分别为：热量0.37,蛋白质0.33,钙0.46,铁0.38,锌0.48,均有统计学意义（P＜0.05）。结论：微量元素及营养的合理摄入有助于促进学龄前儿童身高及体重的增长,若摄入不足或过量将对儿童的生长发育产生负面影响。     

三品种实验用小型猪繁殖性能测定

目的 选取饲养于上海地区的巴马小型猪、贵州小型猪和版纳小型猪,分别测定三品种母猪和公猪的繁殖性能.方法 采用场内结合实验室测定的方法,分别测定初情年龄、初配年龄等母猪繁殖性能指标及性行为年龄、初配年龄等公猪繁殖性能指标.结果 用单因素方差分析比较.结果对比巴马小型猪、贵州小型猪和版纳小型猪的母猪繁殖性能10个指标,贵州小型猪和版纳小型猪的初生窝重和断奶成活率存在显著差异(P＜0.05).分析三品种的公猪繁殖性能,群体间性行为年龄存在显著差异(P＜0.05);三品种初配年龄比较接近;比较初配年龄和初配体质量,表明三品种的小型猪在相同初配年龄时,巴马小型猪个体偏小,版纳小型猪的体质量最大.结论 系统测定了雌性和雄性巴马小型猪、贵州小型猪和版纳小型猪的繁殖性能,为实验用小型猪提供了遗传育种工作的基础,也为小型猪实验动物化提供了背景数据.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…