大鼠小脑旁绒球的传入纤维投射

目的：应用荧光金作为逆行标记物，追踪大鼠小脑旁绒球的传入纤维联系。方法：将荧光金注射到大鼠一侧旁绒球，然后全脑做连续冠状冰冻切片，荧光显微镜下观察结果。结果：荧光金标记神经细胞分布于同侧的下橄榄核内，包括下橄榄内侧核、下橄榄内侧核A亚核、下橄榄内侧核C亚核、下橄榄核主核；双侧前庭神经内侧核、脊髓前庭核、蜗神经腹侧核前部、前置核、脑桥核等，对侧的标记神经细胞多于同侧。结论：大鼠小脑旁绒球与脑干各核区的纤维联系与灵长类动物基本一致，推测低等动物也具有平稳眼跟踪运动和前庭眼动反射的传导环路。     

下丘传入纤维的中脑外起源

用荧光金（ＦＧ）逆行束路追踪技术对１５只大鼠下丘传入纤维的中脑外起源进行了研究，将ＦＧ注入一侧下丘后，在下列核区内可见到ＦＧ标记细胞：双侧下橄榄核（ＩＯＭ）、外侧上橄榄核（ＬＳＯ）；双侧（以同侧为主）蓝斑（ＬＯＣ）、听皮质（ＡＣ）；同侧内侧膝状体（ＭＧ）、内侧上橄榄核（ＭＳＯ）、橄榄旁侧（ＳＰＮ）、斜方体腹侧核（ＶＴＢ）、未定带（ＺＩ）、穹窿周核（ＰＥＦ）；对侧蜗神经腹前核（ＡＶＣＮ）、蜗神经腹后核（ＰＶＣＮ）、蜗神经背侧核（ＤＣＮ）、斜方体外侧核（ＬＴＢ）、三叉神经脊束核（ＮＴＳＴ）。结果提示，下丘不仅是听觉传导路的重要中继核，而且可能是多种功能的整合中枢。     

大鼠脑干向黑质的传入纤维联系

目的:研究大鼠脑干内核团向黑质的传入纤维联系.方法:将荧光金(Fluoro-Gold,FG)泳入大鼠一侧黑质,观察双侧脑干内的FG标记细胞分布.结果:在泳入区同側上丘、臂旁核、中脑中央灰质外侧部、被盖腹侧区;双侧的中缝正中核、中缝背核、艾魏核、脚桥被盖核、蓝斑见到FG逆行标记细胞.结论:大鼠脑干内的艾魏核等多个核团存在向黑质的传入纤维联系.     

大鼠中枢神经系统对脾脏神经支配的调控--假狂犬病毒跨突触标记

为探讨大鼠中枢神经系统与脾脏之间的神经通路联系及大鼠中枢神经系统对脾脏免疫功能的调控机制,采用免疫组化法及荧光金(Flouro-Gold, FG)神经追踪法研究了假狂犬病毒(PRV)及FG注入脾脏72小时、96小时后逆行标记的FG神经元在腹腔神经节内、跨突触标记的PRV神经元及神经纤维在脾脏及神经系统内的分布.结果:①各时间组大鼠脾被膜及实质内均见PRV标记的呈串珠状的神经纤维,有的纤维以终末与免疫细胞密切接触.②各时间组大鼠下胸段(T10～12)脊神经节实质内见PRV标记阳性神经元,被膜内及实质的神经元之间可见 PRV阳性神经纤维.③各时间组大鼠腹腔神经节内见散在的FG标记神经元.④各时间组大鼠中胸段(T5～9)脊髓侧角中间外侧柱,后角背侧固有核及胶状质,延髓迷走神经背核和孤束核内均见PRV标记阳性神经元胞体及突起.⑤96小时组大鼠除上述阳性结果外,标记细胞还可见于下丘脑室旁核.由此推测神经系统与脾脏之间可能存在下列神经通路联系:①下丘脑室旁核→延髓迷走神经背核→脊髓侧角中间外侧柱的交感神经节前神经元→交感神经腹腔节神经元→脾脏.②脾内感觉神经纤维→脊神经节感觉神经元→脊髓后角背侧固有核和胶状质联络神经元→延髓孤束核神经元→下丘脑感觉中枢.     

大鼠延髓和脊髓背角的RKCγ阳性神经元向孤束核投射

目的：观察延髓和脊髓背角内蛋白激酶Cγ亚单位（PKCγ）阳性神经元向孤束核的投射。方法：荧光金（FG）逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术。结果：PKCγ阳性神经元主要分布于延髓和脊髓背的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核,将FG注入孤束核后,在延髓和背髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核内可见FG标记神经元;部分FG标记神经元呈PKCγ阳性;FG／PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核。结论：延髓和脊髓背角的PKCγ阳性神经元可能参与将躯体和内脏伤害性刺激信息向孤束核的传递。     

猫听觉脑干上橄榄复合体投射神经元的分布

目的:探讨猫上橄榄复合体各个核团内橄榄耳蜗神经元的分布和形态.方法:将8只成年猫随机分成两组,实验组5只,于左侧耳蜗内注射霍乱毒素B亚单位(cholera toxin B subunit,CTB),右侧耳蜗注射荧光金(fluoro-gold,FG);对照组3只,于双侧耳蜗注射生理盐水.取脑干组织切片,经免疫组化ABC方法和DAB显色后观察被标记的橄榄耳蜗神经元.结果:实验组猫橄榄耳蜗神经元总数为2518个,其中外侧橄榄耳蜗(lateral olivocochlear,LOC)神经元1738个,占69.0%,主要分布于脑桥中部,以同侧投射占优势.内侧橄榄耳蜗(medial olivocochlear,MOC)神经元780个(31.0%),主要位于背内侧橄榄旁核(dorsomedial periolivary nucleus,DMPO)、内侧斜方体核(medial nucleus of the trapezoid hody,MNTB)以及腹侧斜方体核(ventral nucleus of the trapezoid body,VNTB),在脑桥嘴侧分布密集,其发出的纤维以对侧投射占优势.结论:猫的橄榄耳蜗神经元分布LOC神经元向同侧投射为主;而MOC神经元则为对侧投射优势,且MOC神经元分布较LOC神经元更靠近脑桥嘴侧.     

大鼠间脑和脑干向颞叶听皮质纤维投射的实验性研究

应用荧光金（ＦＧ）逆行追踪技术，探索了大鼠颞叶听皮质的传入纤维投射。向一侧颞叶听皮质注入ＦＧ后，可在间脑和脑干的下列核区内见到ＦＧ逆行标记细胞：丘脑室旁核、丘脑外侧背核、丘脑腹后内侧核、丘脑网状核、无名质、连合核、菱形核、外侧膝状体核、内侧膝状体核、中缝背核、中缝线形核、脑桥中缝正中核和蓝斑核。     

大白鼠上丘水平导水管周灰质外侧区的传入联系

HRP注入大白鼠上丘水平导水管周灰质外侧区后,标记细胞出现在下列脑区:前额内侧皮质,额顶运动皮质,额顶体感皮质,扣带前、后皮质,纹状皮质17、18区,颞叶听皮质,下托及海马1、2区:缰核、丘脑室旁核、背内侧核、前背核、束旁下核、未定带,下丘脑前区、背侧区,下丘脑背、腹内侧核,乳头体上核,背、腹侧乳头体前核,下丘脑外侧区:中缝背核、被盖背外侧核、中缝正中核、黑质网状部,外侧丘系腹侧核及二叠体旁核;兰斑、上橄榄核、第四脑室底灰质、脑桥尾侧网状核、三叉神经脊束核、外侧网状核小细胞部、薄束核及楔束核     

大鼠延髓和脊髓背角的PKCγ阳性神经元向孤束核投射

目的观察延髓和脊髓背角内蛋白激酶Cγ亚单位(PKCγ)阳性神经元向孤束核的投射. 方法荧光金(FG)逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术. 结果 PKCγ阳性神经元主要分布于延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核;将FG注入孤束核后,在延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核内可见FG标记神经元;部分FG标记神经元呈PKCγ阳性,FG/PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ,Ⅱ,Ⅲ层及脊髓的外侧脊核. 结论延髓和脊髓背角的PKCγ阳性神经元可能参与将躯体和内脏伤害性刺激信息向孤束核的传递.     

第12次全国中青年医学(耳鼻喉科)学术交流会一等奖论文简介(续)

《上橄榄复合体向下丘的GABA能投射》邱建华,男,38岁,第四军医大学西京医院副教授。采用辣根过氧化物酶(HRP)逆行追踪和免疫纽化ABC技术的研究结果表明,在豚鼠上橄榄复合体有γ-氨基丁酸(CABA)阳性神经元和终末分布,并具有较明显的特征性。主要分布在双侧的上橄榄外侧核(LSO)、背侧核(DNTB),及上橄榄周围核(PN)内,阳性点状终末主要分布在斜方体腹侧核(VNTB)和斜方体外侧核(LNTB),以VNTB数量较多。LSO和PN内均发现HRP—GABA双标记细胞,约占标记细胞的1/3。上橄榄内侧和外侧系     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

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