大鼠中缝背核、正中隆起接触脑脊液神经元化学性质的研究

用CB-HRP追踪与免疫细胞化学结合的方法,对大鼠中缝背核、正中隆起接触脑脊液神经元的化学性质进行了研究。将CB-HRP注入第三脑室后,中缝背核、正中隆起内观察到CB-HRP标记细胞。标记细胞分布于中缝背核的腹侧部(在中脑水管腹侧呈对称性分布)及正中隆起的室管膜带。在CB-HRP与5-羟色胺(5-HT)免疫细胞化学结合的切片上,中缝背核及正中隆起内出现三种标记细胞:HRP单标细胞、5-HT免疫反应阳性细胞、HRP/5-HT双标细胞,双标细胞中为中小型、三角形或圆形。上述结果提示:中缝背核、正中隆起存在着5-HT能触液神经元。     

伸长细胞(综述)

中枢神经系统的脑室壁,存在一种特殊的室管膜细胞。主要位于第3脑室底和腹侧壁,是丘脑下部正中隆起的主要组成成分之一。这些细胞顶部接触脑脊液,底部伸出长的突起至正中隆起的外层、脑垂体门脉系前毛细血管和弓状核、腹内侧核的毛细血管袢     

大鼠室周器官内小胶质细胞在实验性变态反应性脑脊髓炎不同时期的形态学观察

室周器官(circumvent ricular organs,CVOs)是指由室管膜特化形成的位于第三、四脑室壁上的一些微小器官,包括穹窿下器、正中隆起等.小胶质细胞是中枢神经系统中主要的宿主免疫细胞,关于CVOs处小胶质细胞的分布尚缺乏形态学报道.     

垂体前叶直接神经支配的免疫组织化学研究

经典的慨念认为下丘脑的神经内分泌细胞轴实终止于正中隆起,并释放生物活性物质进入正中隆起毛细血管,通过垂体门脉系统运输到垂体前叶,调节前叶分泌腺细胞的功能。虽有报告说少量儿茶酚胺神经纤维支配前叶的血管,但垂体前叶腺细胞没有神经支配。     

正常成年大鼠中枢神经系统内nestin蛋白免疫阳性结构的分布

目的：观察作为神经前体细胞标记物-中间丝蛋白nestin在成年大鼠中枢神经系统内的分布特征．方法：多聚甲醛灌流固定的脑和脊髓组织切片上，进行免疫组织化学(ABC法)染色．结果：nestin免疫阳性主要分布于海马齿状回、室管膜及室管膜下区、穹隆下器、正中隆起、最后区等室周器官、以及脑干和脊髓的中缝胶质结构等区域．nestin反应产物定位在胞体、及其长短不等的星状突起或放射状突起内．结论：结果提示，在成年鼠脑和脊髓的上述区域内，可能仍然存在一定数量呈nestin免疫反应的神经前体细胞，其生理学意义值得进一步探讨。     

大白鼠正中隆起栅状带超微结构的研究

本文用醛-锇酸固定法对大白鼠正中隆起栅状带的超微结构进行了研究。结果表明,正中隆起柵状带内有大量的毛细血管、神经终末和伸展细胞的基突ぷ创拿秆苣谄は赴胧头?或释放抑制)激素的转运关系密切。     

大鼠室周器官的形态学观察

目的 系统观察5个室周器官的微细结构。方法 对40只成年SD大鼠行脑切片，分别行硫堇和Tolivia还原银染色，以及NADPH-d组织化学染色。结果 （1）室周器官微小的体积与其异常丰富的血床形成鲜明的反差；（2）5个室周器官依体积大小次序为穹隆下器，最后区，正中隆起，连合下器，终板血管器；（3）穹隆下器和终板血管器可能含神经分泌细胞；（4）连合下器具有室管膜下神经细胞层；（5）正中隆起具有最密集和最多的伸展细胞。一氧化氮合酶均呈阳性；（6）在最后区与延髓中央管之间，可能有神经纤维联系。结论 室周器官可能是脑内体液化学信息的重要转导位点。     

SOX2在成年小鼠室周器官细胞的表达及其意义

目的探讨SOX2在成年小鼠室周器官(CVOs)细胞的表达及其意义。方法应用免疫组织化学法检测成年C57BL/6小鼠脑CVOs细胞中SOX2的表达情况。结果 SOX2在最后区(AP)、正中隆起(ME)、穹窿下器(SFO)、联合下器(SCO)和脉络丛(ChP)细胞均有强表达,且室管膜上皮细胞几乎都100%阳性表达SOX2,除SCO外,CVOs表达SOX2阳性的细胞数量明显少于侧脑室的室下区表达SOX2阳性的细胞数量(P<0.05)。结论在成年小鼠脑CVOs分布有大量的SOX2阳性细胞,表明成年小鼠CVOs可能存在具有增殖和分化潜能的神经干细胞和/或神经祖细胞。     

金黄鼠室管膜上神经元复合体催产素能免疫细胞化学研究

脑室周围的神经元胞体和突起部分构成的系统,即室管膜上神经元复合体(SENC),位于金黄鼠的第三脑室底和神经垂体.应用免疫细胞化学技术对SENC中神经元成分进行研究。SENC中是否存在催产素和血管加压素。研究发现催产素能神经纤维到达正中隆起室管膜上并进入SENC。一些神经纤维穿过SENC到达室管膜表面和终止于第三脑室底,同时,其它神经终末终止于SENC的神经毡。这些催产素能神经纤维分泌催产素进入脑脊液。血管加压素神经纤维仅在四个实验标本中发现有一例SENC中存在。推测它来自下丘脑—垂体束的分支。SENC中既没有发现有催产素神经元,又没有发现血管加压素神经元。SENC的功能还不清楚,但是,它可能参与神经内分泌调节,在调节过程中,脑脊液中的催产素对血压,血管反射产生影响。     

妇科神经内分泌的几个问题

<正> Harris于1937年发现丘脑下部正中隆起处分泌神经内分泌物质。这一发现表明丘脑下部既具有神经特性,又具有内分泌功能,从而出现“神经内分泌”一词。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.