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1.
目的:了解吸烟艰多环芳烃接触者生物膜损伤指标、染色体畸变和血清癌基因蛋白P21的影响。方法:P对接触多环芳烃化合物的24名焦炉工和27名沥青工中吸烟与不吸烟者的血清谷胱甘肽S-转移酶(GST)、血清脂质结合唾液酸(LSA)、染色体畸变和血清癌基因蛋白P21水平进行研究。结果:吸烟与不吸烟者间GST、LSA、染色体畸变率和血清P21水平各指标差异均无显著性差异(P〉0.05)。结论:吸烟对多环芳烃接  相似文献   

2.
目的 :了解多环芳烃接触者遗传指标和血清癌基因蛋白P2 1表达水平及其在肺癌早期诊断中的意义 ,为多环芳烃作业者的劳动保护提供科学依据。方法 :对接触多环芳烃化合物的焦炉工 2 4名、沥青工 2 7名和健康对照组 31名的染色体畸变率和血清癌基因蛋白P2 1水平进行了检测。结果 :染色体异常检出率 ,焦炉工和沥青工均显著高于对照组 (P <0 .0 0 5 ) ;染色体裂隙次数 ,焦炉工和沥青工均显著高于对照组 (P <0 .0 1) ;染色体结构、数目畸变数 ,焦炉工显著高于对照组 (P <0 .0 1)。血清P2 1水平 ,焦炉工、沥青工均显著高于对照组 (P <0 .0 5 ) ,沥青工显著高于焦炉工 (P <0 .0 5 )。结论 :多环芳烃化合物具有遗传毒性 ,并可致血清P2 1蛋白水平升高。  相似文献   

3.
多环芳烃接触者染色体畸变和血清P21水平检测   总被引:2,自引:0,他引:2  
目的:了解多环芳烃接触者遗传指标和血清癌基因蛋白P21表达水平及其在肺癌早期诊断中的意义。为多环芳烃作业者的劳动保护提供科学依据。方法:对接触多环芳烃化合物的焦炉工24名、沥青工27名和健康对照组31名的染色体畸变率和血清癌基因蛋白P21水平进行了检测。结果:染色体异常检出率,焦炉工和沥青工均显著高于对照组(P〈0.005);染色体裂隙次数,焦炉攻沥青工均显著高于对照组(P〈0.05),染色体系结  相似文献   

4.
目的 :探讨血清P5 3蛋白作为职业性肺癌早期监测指标的可能性和应用价值。方法 :对长期接触多环芳烃混合物 (PAHs)的 2 3名焦炉工和 18名沥青工进行外周血淋巴细胞染色体畸变检查初筛 ,检出染色体畸变者各16名 ,然后采用双抗夹心酶联免疫吸附试验方法 (Sandwich ELISA)测定畸变者血清P5 3蛋白水平。以 12名正常献血员为对照组。结果 :焦炉工和沥青工血清P5 3水平均值高于对照组 (P <0 .0 5 ) ,其中 10例高于对照组 ( x 2s)。结论 :血清P5 3水平可从基因水平反映PAHs对机体遗传物质的损伤作用 ,该指标有可能成为职业性肺癌早期监测的又一途径。  相似文献   

5.
血清P53蛋白检测对职业性肺癌的预报价值   总被引:6,自引:0,他引:6  
目的:探讨血清P53蛋白作为职业性肺癌早期监测指标的可能性和应用价值。方法;对长期接触多环芳烃混合物(PAHS)的23名焦炉工和18名沥青工进行外周血淋巴细胞染色体畸变检查初筛,检出染色体畸变者各16名,然后采用双抗夹心酶联免疫吸附试验方法9Sandwich-EKLISA)测定畸变者血清P53蛋白水平。以12名正常献血员为对照组。结果:焦炉工和沥青工血清P53水平均值高于对照组(P〈0.05),其  相似文献   

6.
焦炉工染色体畸变和血清p53蛋白水平的检测   总被引:1,自引:0,他引:1  
目的:探讨职业性肺癌早期监测指标染色体畸变及血清p53蛋白的应用价值。方法:选择长期接触多环芳烃混合物(PAHs)的23名焦炉工为研究对象,另选12名非暴露者为对照,进行外周血淋巴细胞染色体畸变检查,对有畸变者采用双抗夹心酶联免疫吸附试验(Sandwich-ELISA)测定血清p53蛋白水平。结果:焦炉工染色体异常检出率高于对照组(P<0.05),畸变类型为单体型,以裂隙为主;焦炉工血清p53水平均值高于对照,但差异无显著性,其中5例血清p53水平高于对照组x+2S,判为阳性;两指标间无相关关系。结论:染色体畸变及血清p53水平可从不同途径反映PAHs对机体遗传物质的损伤作用,两者的联合应用是提高肿瘤预报性的有效途径  相似文献   

7.
目的 :探讨肺癌发病机制及早期监测指标 ,为多环芳烃作业人员劳动保护提供科学依据。方法 :对 5 1名多环芳烃类混合物接触者 (接触组 ) ,18例非肺癌患者 ,2 5例肺癌患者和 31名健康人员的血清脂质结合唾液酸(LSA)、谷胱甘肽S 转移酶 (GST)活性、超氧化物歧化酶同工酶 (SOD ,MnSOD和CuMnSOD)和T淋巴细胞亚群 (T3,T4,T8及T4/T8)进行测定。结果 :接触组、肺癌组和非肺癌组LSA均显著高于对照组 (P <0 .0 1)。接触组、非肺癌组和肺癌组GST显著高于对照组 (P <0 .0 5 )。肺癌组SOD显著高于非肺癌组 (P <0 .0 5 )。接触组、肺癌组和非肺癌组T3均显著低于对照组 (P <0 .0 5 ) ,接触组T3显著高于非肺癌组和肺癌组 (P <0 .0 5 ) ;接触组、非肺癌组和肺癌组T4均显著低于对照组 (P <0 .0 5 ) ,接触组T4显著高于非肺癌组 (P <0 .0 5 ) ;非肺癌组T4/T8显著低于对照组 (P <0 .0 1) ,接触组T4/T8显著高于非肺癌组 (P <0 .0 5 )。结论 :多环芳烃接触者生物膜出现了损伤性改变 ,免疫功能受到抑制。  相似文献   

8.
目的:为了解接触焦炉逸散物职业性人群遗传效应的改变,从分子水平揭示焦炉逸散物的遗传毒作用.方法:对25名焦炉工和12名正常对照分别检测了血清P21和外周血淋巴细胞姐妹染色单体互换(SCE)率.结果:发现焦炉工P21水平(3 774.2)、SCE率(11.43)明显高于对照组P21水平(1 270.7)和SCE率(6.37);焦炉工中,炉顶工血清P21均值(4 377.5)高于其它工种(3 300.1),炉顶工SCE(13.51)明显高于其它工种(10.07);对焦炉工按是否吸烟进行比较,未发现组间P21和SCE有差异显著性.结论:提示焦炉逸散物具有较强的遗传毒理学效应,可提高机体癌蛋白P21水平和SCE频率.  相似文献   

9.
煤焦沥青接触者血清SOD、MDA及唾液酸检测   总被引:3,自引:0,他引:3  
目的:检测煤焦沥青接触者血清超氧化物歧化酶总量(SOD)、丙二醛(MDA)及唾液酸(SA)水平,探讨职业性肺癌的早期预警指标.方法:焦炉工55人(接触组),某高校教师、学生及职工40人(非接触组),采用硫代巴比妥酸法测定其血清MDA水平,采用黄嘌呤氧化快速比色法测定T-SOD水平,采用比色法测定SA水平.结果:接触组血清MDA、SA含量高于非接触组(P<0.001),血清T-SOD含量低于非接触组(P<0.001).接触组不同年龄及工龄组之间各指标差异均无统计学意义(P>0.05).结论:煤焦沥青可导致接触者生物膜的明显损伤.SOD、MDA、SA等指标可用于职业性肺癌的早期预警.  相似文献   

10.
目的:为筛选职业性肺癌高危人群的早期预报指标,对19例焦炉工和12例对照进行了研究。方法:用外周血淋巴细胞培养法检测了外周血淋巴细胞的姐妹染色体互换(SCE)。结果:焦炉工 SCE 率明显高于对照;焦炉工中吸烟与不吸烟者 SCE 间无明显差异。结论:焦炉逸散物具有明显的遗传毒理学效应,对焦炉工染色体可造成一定的损伤。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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