两色紫金牛化学成分研究

目的 研究两色紫金牛的活性化学成分.方法 应用柱色谱分离化学成分,波谱方法鉴定化合物结构.结果 从两色紫金牛脂溶性部位得到5个化合物,分别鉴定为岩白菜素(1),11-O-(3‘,5‘-二羟基-4‘-甲氧基没食子酰基)-岩白菜素(2),11-O-(3‘,4‘,5‘-三羟基没食子酰基)-岩白菜素(3),β-谷甾醇(4)和豆甾醇(5).结论 化合物1～5均为首次从该种植物中分离得到,其中化合物2为首次从该属植物中分离得到.     

紫花地丁化学成分研究

目的 研究紫花地丁Viola yedoensis 药效活性部位醋酸乙酯提取物的化学成分。方法 利用现代化学成分分离纯化技术进行分离纯化,根据化合物的理化性质和光谱数据进行结构鉴定。结果 从该部位分离鉴定了15个化合物,分别鉴定为木犀草素(1)、芹菜素(2)、槲皮素(3)、5,7-二羟基3,6-二甲氧基黄酮(4)、柚皮素(5)、芦丁(6)、7-羟基-8-甲氧基香豆素(7)、秦皮乙素(8)、咖啡酸(9)、2-羟基-1-(4-羟基-3-甲氧基)苯基-1-丙酮(10)、3,4-二羟基苯甲酸(11)、3-羟基-4-甲氧基苯甲酸甲酯(12)、奎宁酸(13)、4-muurolene3,10-diol(14)、β-谷甾醇(15)。结论 除化合物8和15外,其余13个化合物均为首次从该植物中分得。化合物4、5、7、9～14为首次从该属植物中分得。     

蔓荆子黄酮类化学成分的研究

目的 研究蔓荆子中的黄酮类化学成分.方法 采用Sephadex LH-20色谱柱、聚酰胺柱色谱等手段进行分离纯化,通过理化性质和波谱解析对结构进行鉴定.结果 从蔓荆子75%(体积分数)乙醇提取液中分离得到6个化合物:蒿黄素(1)、4'-羟基-5,6,7-三甲氧基黄酮(2)、蔓荆子黄素(3)、5,4'-二羟基-6,7,-二甲氧基黄酮(4)、山奈酚(5)、槲皮素(6).结论 化合物2和化合物4为首次从该植物中分离得到.     

狼毒大戟化学成分研究

目的研究狼毒大戟化学成分。方法采用硅胶柱层析进行分离纯化化合物,根据理化性质和现代波谱技术进行结构鉴定。结果从狼毒大戟根的石油醚部分分离出5个化合物,分别鉴定为2,4-二羟基-6-甲氧基-3-甲基苯乙酮(1)、3,3′-二乙酰基-4,4′-二甲氧基-2,2′,6,6′-四羟基二苯甲烷(2)、2,4-二羟基-3-醛基-6-甲氧基苯乙酮(3)、富马酸(4)、β-谷甾醇(5)。结论5个化合物均为已知化合物,化合物3为首次从该植物中获得。     

直立百部的化学成分

目的:研究直立百部Stemona sessilifolia(Miq.) Miq.根中的化学成分.方法:运用各种色谱方法进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构.结果:从直立百部中分离得到9个化合物,分别鉴定为Protostemotinine (1)、Stemospironine (2)、Stilbostemin B (3)、Stilbostemin D (4)、4'-Methylpinosylvin(5)、芝麻素(6)、28-羟基-正二十八烷酸-3'-甘油单酯(7)、26-羟基-正二十六烷酸-3'-甘油单酯(8)及β-谷甾醇(9).结论:化合物2～8为首次从该植物中分离得到.     

仙人掌的化学成分研究

目的:研究仙人掌的化学成分.方法:采用多种层析手段进行分离纯化,应用多种波谱技术,结合文献对照,对分离得到的化合物进行结构鉴定.结果:从仙人掌肉质茎的乙醇提取物中分离出6个化合物,其结构分别鉴定为3,4-二羟基苯甲酸乙酯 (1),3,4-二羟基苯甲酸 (2),槲皮素-3-O-甲基-7-O-β-D-葡萄糖苷 (3),山奈酚7-O-β-D-葡萄糖苷 (4),manghaslin (5)和山奈酚7-O-β-D-葡萄糖基 (1→4)-β-D-葡萄糖苷(6).结论:化合物6为新化合物,化合物1～5均为首次从本属植物中分离得到.     

窄头橐吾化学成分的研究

目的研究窄头橐吾根部的化学成分。方法采用柱层析(CC)对窄头橐吾根部的提取物进行分离提纯,并利用现代波谱方法(1H-NMR,13CNMR,HMBC,MS,IR)分析鉴定,确定其化合物的结构。结果从该植物的乙酸乙酯部分分离出5个化合物,它们的结构分别鉴定为:木栓醇(1),木栓酮(2),2-异丙基-5-乙酰基-6-羟基苯并呋喃(3),5,6-二甲氧基-2-异丙基苯并呋喃(4),谷甾醇(5)。结论化合物1~5均为已知化合物,并首次从该植物中分离而得。     

乌苏里藜芦茎叶化学成分研究

目的对乌苏里藜芦Veratrum nigrum var.ussuriense地上部分的化学成分进行研究。方法通过溶剂分配和反复硅胶柱色谱进行化合物的分离、纯化,并利用质谱和核磁共振(包括1D和2D)等波谱技术鉴定化合物的结构。结果分离鉴定了4个化合物,分别鉴定为6-羟基-7-甲氧基-2-(3′-羟基-2′,5′-二甲氧基苯基)苯骈呋喃(Ⅰ)、白藜芦醇(Ⅱ)、3,5,2′,4′-四羟基茋(Ⅲ)、β-谷甾醇(Ⅳ)。结论化合物Ⅰ为新化合物,命名为藜芦呋喃素;化合物Ⅱ～Ⅳ为首次从该植物的地上部分中分离得到。     

毛杭子梢化学成分的研究

目的研究毛杭子梢Campylotropis hirtella根60%乙醇提取物的化学成分。方法运用多种色谱学方法对毛杭子梢60%乙醇提取物的化学成分进行分离,并根据光谱数据对化合物进行结构解析。结果从该植物中分离得到6个化合物,鉴定为(3S,5R,8R)-3,5-二羟基-megastigman-6,7-亚烯-9-酮-5-O-β-D-吡喃葡萄糖苷(Ⅰ)、开环异落叶松脂素-9′-O-β-D-吡喃葡萄糖苷(Ⅱ)、2,6-二甲氧基-4-羟基苯酚1-O-β-D-葡萄糖苷(Ⅲ)、丁香醛(Ⅳ)、3,5-二甲氧基-4-羟基苯酚1-O-β-D-芹糖基-(1→6)-O-β-D-葡萄糖苷(Ⅴ)、胡萝卜苷(Ⅵ)。结论化合物Ⅰ～Ⅵ均为首次从该属植物中分离得到。     

红树伴生植物相思子的化学成分研究

目的 研究红树伴生植物相思子Abrus precatoriusL.茎叶的化学成分.方法 采用硅胶柱色谱、凝胶Sephadex LH-20柱色谱等手段对相思子茎叶的化学成分进行分离,应用光谱分析方法并结合文献对化合物进行结构鉴定.结果 从相思子茎叶中分离得到7个化合物,经鉴定分别为3-吲哚甲酸(1)、尿嘧啶(2)、N-[2-(1,3,4-三羟基-正二十烷基)]-正十六碳酰胺(3)、芒柄花素(4)、异亮氨酸(5)、豆甾-4,22-二烯-3β,6β-二醇(6)、△5.22豆甾醇-3-O-β-D吡喃葡萄糖苷(7).结论 化合物2～7首次从该植物中分离得到.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.