等离子体处理对有机硅膜气体透过性能的影响

本文研究了有机硅膜经等离子体处理和等离子体聚合沉积后,气体透过性能的变化。以及放置一段时间后,随着等离子体处理效果的变化,膜的气体透过性能的改变。结果表明无论是Ar等离子体处理的有机硅膜,还是八甲基环四硅氧烷(D_4)等离子体聚合沉积的有机硅膜,其气体透过性能都发生了明显的变化。即经等离子体处理后,膜的气体透过系数下降,选择分离系数上升。在放置一段时间后,其气体透过系数和选择分离系数均表现出有回复的趋势。因此,等离子体处理对膜的气体透过性能的影响随放置时间而变化。     

不同等离子体处理氧化锆对人牙龈成纤维细胞黏附能力的影响

目的: 比较以不同种类气体为工作气体的大气压放电冷等离子体处理氧化锆对氧化锆表面人牙龈成纤维细胞生物学行为的影响。方法: 将20个氧化锆试件随机分为4组:对照组(无处理),氦气大气压介质阻挡放电等离子体处理90 s组(He组),氩气大气压介质阻挡放电等离子体处理90 s组(Ar组),95%氩气+5%氧气大气压介质阻挡放电等离子体处理90 s组(Ar+O₂组),每组5个。分组处理后观察氧化锆表面形貌,测定表面粗糙度及润湿性,分析表面元素组成,培养人牙龈成纤维细胞3 h测定细胞在氧化锆表面的初期黏附能力,免疫荧光染色观察细胞黏附初期细胞形态。结果: 等离子体处理后氧化锆表面形貌及粗糙度无明显改变,接触角均显著降低(P<0.05), 其中Ar组及Ar+O₂组接触角与He组具有统计学意义(P<0.05)。X射线光电子能谱分析结果显示,等离子体处理后氧化锆表面C/O比分别下降至0.45(He组)、0.36(Ar组)和0.33(Ar+ O₂组)。经Ar及Ar+O₂混合气体等离子体处理的氧化锆表面人牙龈成纤维细胞黏附初期数量提高,差异具有统计学意义(P<0.05)。相对于对照组,不同等离子体处理后的氧化锆表面成纤维细胞黏附及增殖情况更佳,细胞在黏附初期形态铺展更佳,伸出伪足更多。结论: 不同种类大气压放电冷等离子体处理氧化锆可在不改变材料表面形貌及粗糙度的情况下通过在材料表面引入含氧官能团提高材料表面润湿性;以Ar+O₂混合气体为工作气体的大气压放电冷等离子体可显著提高人牙龈成纤维细胞在氧化锆表面的早期黏附能力。     

双极等离子体电切镜治疗膀胱肿瘤76例报告

目的探讨利用双极等离子体电切镜治疗膀胱肿瘤的优点。方法本组均采用双极等离子体电切镜进行电切，以生理盐水作为介质。结果本组病人手术时间为20～40min（25．3＋6．2min），术中出血约为2—20ml（12＋6．2m1）。术后给予抗炎治疗3天，4—7天拔除尿管，术后病人均未出现膀胱痉挛，无出血。平均住院日数9天。结论双极等离子体电切刀为双极，有自体回流，不导电，可以使用生理盐水作为介质，不易出现闭孔反射。术后病人恢复快，不产生膀胱痉挛，出血少，病人痛苦小，住院时间短。对于早期膀胱肿瘤，采用双极等离子体电切治疗不失为最好的方法。     

低频RF-等离子体处理对医用不锈钢表面润湿性能的影响

以聚乙烯-乙烯醇（EVAL）为模型高分子，研究了等离子体处理前后不锈钢表面EVAL溶液的铺展润湿情况、EVAL膜的形貌及其结合强度。并建立了医用不锈钢表面润湿性和表面自由能、表面结构之间的关系。研究结果表明：等离子体处理后，不锈钢表面的铺展润湿性显著提高。等离子体处理的最佳工艺条件为：氮气，偏压100V，时间10min。最佳条件处理后，不锈钢表面EVAL膜均匀性、致密度以及涂层与基体的结合强度均得到明显提高。反射红外（ATR—FTIR）、原子力显微镜（AFM）、X光电子能谱（XPS）分析结果显示Ar、N2气体等离子体，尤其是N2气体等离子体预处理后，材料的表面自由能（尤其是极性分量）显著增大，润湿性增强。极性分量的增加与等离子体时材料表面的清洗、刻蚀以及活性化有关。     

低温等离子体对细菌芽孢的灭活研究

目的 探讨低温等离子体对细菌芽孢的灭活效果及相关机制.方法 采用介质阻挡放电产生大气压低温等离子体,对枯草杆菌黑色变种芽孢进行处理,设定3个放电电极间距和7个处理时间,采用平板记数法记数存活芽孢数.并计算杀灭对数值(KLV).另外,研究高压电场灭菌效果并对低温等离子体处理后的枯草杆菌黑色变种芽孢进行透射电镜观察,用以初步探讨低温等离子体对细菌芽孢的灭活机制.结果 在电极间距分别为3、4和5 mm时.低温等离子体对枯草杆菌黑色变种芽孢的KLV超过5时的处理时间依次为25、30、35 s.机制研究发现.低温等离子体中的高压电场效应对枯草杆菌黑色变种芽孢的杀灭效果很差:透射电镜结果显示,低温等离子体对枯草杆菌黑色变种芽孢表面和内部结构都有损伤作用.结论 低温等离子体对细菌芽孢有良好的杀灭效果,且具有时间剂量依赖性.带电粒子的击穿作用和活性氧的氧化作用可能在低温等离子体杀灭芽孢的过程中起主要作用.而低温等离子体中的高压电场效应可以忽略不计.

Abstract：

Objective To investigate the effect of low-temperature plasma on inactivation of bacterial spores and explore the mechanism. Methods Dielectric barrier discharge (DBD) was employed to generate the atmospheric low-temperature plasma for treatment of B.subtilis var. niger spores with the gas spacing of 3,4 and 5 and treatment time intervals of 5, 10, 15, 20, 25, 30 and 35 s. The survived colonies was counted with plate counting method, and the killing log value (KLV) at different treatment times was calculated. The inactivation effect of electric field on B.subtilis var.niger spores was also investigated and the spores treated with low-temperature plasma were observed with transmission electron microscope. Results With the gap spacing of 3, 4 and 5 mm, the KLV of low-temperature plasma on B.subtUis var.niger spores within 25, 30 and 35 s of exposure was more than 5. The germicidal effects of the electric field on B. subtilis var.niger spores were rather poor. Transmission electron microscopy demonstrated total destruction of the surface and interior structure of the spores by low-temperature plasma. Conclusions Low-temperature plasma is effective for inactivation of the bacterial spores with a time and dose dependence. The penetrating effect of charged particles and oxygenation effect of the reactive oxygen species might play a dominant role in plasma-induced bacterial spore inactivation, while the role of electric field is negligible.     

HT-7装置中低杂波电流驱动时的电子温度

在HT-7托卡马克实验装置中采用低杂波电流驱动,在不同低杂波功率和电子密度(ne)下,考察中心电子温度和能量约束时间的变化。观察到当ne=1.3×1019～2.6×1019 m-3时,中心电子温度随低杂波输入功率的增大而升高;当ne=1.3×1019 m-3,低杂波输入功率为400 kW时,电子温度最高,达到1.37 keV。在本实验参数范围内,其能量约束时间与ITER89P L模定标率十分吻合。比较欧姆加热和低杂波加热下的电子温度分布,当ne=1.71×1019 m-3,低杂波输入功率为260 kW时,电子温度有明显的温度梯度出现。     

人胎回肠树突状细胞的形态发生分析

目的：探索人胎回肠树突状细胞（dendritic cell,DC）的形态发生规律。方法：应用免疫组织化学术和体视学方法对人胎回肠DC的出现时间、形态参数进行分析。结果：第11w时人胎回肠固有层开始出现阳性DC,其数量随胎龄的增大而逐渐增多;回肠固有层及集合淋巴小结（Peyer’s Patches,PP）阳性DC的数密度均随胎龄的增加而增大,而集合淋巴小结DC的数密度比同期固有层的要高（P〈0．05）;平均表面积随胎龄的增加呈现逐渐减小的趋势,而集合淋巴小结DC的平均表面积比固有层的要小（P〈0．001）;集合淋巴小结DC的表面积体积比比固有层的要小（P〈0．05）。结论：人胎回肠DC的数量随胎龄的增加而增多,固有层DC的形状、大小和数量均与集合淋巴小结DC有差异。     

三油楔轴承若干参数对刚性和弹性转子失稳转速的影响

分别以三油楔固定瓦滑动轴承-刚性Jeffcott转子系统和三油楔固定瓦滑动轴承-弹性Jeffcott转子系统为研究对象,采用有限差分法,通过数值计算联立求解系统自由振动方程、Reyn-olds方程和扰动Reynolds方程。研究了轴承的宽径比、最小间隙比和平均油温的变化对两种系统失稳转速的影响规律并进行了比较。结果表明:减小宽径比,弹性系统失稳转速上升,刚性系统则在小范围内有小幅波动;增大最小间隙比,两系统的失稳转速先减小后增大,最小间隙比的变化对刚性系统的稳定性影响更大;提高平均油温,两系统的失稳转速提高。     

氩气低温等离子体处理对口腔修复用PEEK复合材料粘结性能的影响

目的：探讨氩气低温等离子体处理对聚醚醚酮（PEEK）及其复合材料剪切强度的影响，阐明PEEK及其复合材料的粘结机制。方法：将制备的纯PEEK作为对照组，PEEK复合材料作为实验组。将对照组和实验组的试件分别进行0、 5、 15和25 min氩气低温等离子体表面处理(等离子0、5、15和25 min组)。扫描电镜（SEM）观察试件经过处理后表面微观形貌变化。将处理后的试件与RelyXTM Unicem进行粘结，37℃水浴24 h后检测试件的剪切强度，体视显微镜下评价其破坏模式。结果：经过氩气低温等离子体处理后，各组试件表面可见不同程度的凸状沉淀物和犁沟。对照组试件经过低温氩气等离子体处理5 min后，剪切强度值比等离子0 和15 min组明显升高（P<0.01）；等离子25 min组试件的剪切强度值与等离子5 min组比较差异无统计学意义（P>0.05）。实验组试件的剪切强度值，等离子5 min组低于15和25 min组（P<0.05），等离子15 min与25 min组的比较差异无统计学意义（P>0.05）。实验组中等离子15 min组试件的剪切强度值明显高于对照组（P<0.05），其他各组之间比较差异无统计学意义（P>0.05）。各组试件的破坏模式均主要以界面破坏为主。结论：氩气低温等离子体处理能有效地增强PEEK及其复合材料的剪切强度，随着处理时间的延长，剪切强度增大。试件表面硬度不同导致了纯PEEK及其复合材料剪切强度变化趋势的不一致。     

新型复合式浆态床内气体停留时间分布及模型

由空气-水、10%K2CO3溶液、20%K2CO3溶液、液体石蜡油构成气液两相体系,常压下表观气速为1～20 cm/s、静态液位200～550 mm、液体循环量0～240 L/h时,采用脉冲实验法测量一种特殊结构的浆态床反应器内气体停留时间分布.考察了操作参数和液体性质对浆态段的气体停留时间分布及返混程度的影响.结果表明:流型对停留时间分布的影响较大.气体平均停留时间随液体循环量和液体黏度的增加而增大;气体返混程度随静态液位增加而减小,随液体表面张力增加而增大.表面张力是影响返混程度的主要因素.同时建立相应的数学模型,得到停留时间密度分布函数,实验值与模型预测值吻合较好.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.