NB4细胞PML—RARα融合基因表达对维甲酸诱导分化作用的影响

目的研究急性早幼粒白血病（ＡＰＬ）细胞株ＮＢ４细胞ＰＭＬ－ＲＡＲα（早幼粒细胞白血病基因－维甲酸受体基因α）融合基因的表达对维甲酸诱导分化作用的影响，探讨ＰＭＬ－ＲＡＲα融合基因与维甲酸治疗作用间的关系。方法用反义核酸来阻断ＰＭＬ－ＲＡＲα融合基因的表达并用逆转录－聚合酶链式反应检测。ＮＢ４细胞的生长、分化及功能。通过细胞生长曲线、形态学、细胞表面标志及ＮＢＴ（硝基四氮唑蓝）还原试验进行判定，细胞周期应用流式细胞仪进行分析。结果反义核酸阻断ＰＭＬ－ＲＡＲα表达后使维甲酸对ＮＢ４细胞的生长抑制作用增强，Ｓ期细胞由５２％降至２９％，同时ＮＢＴ还原能力提高，细胞表面标志变化明显，提示对维甲酸敏感性增加。结论ＰＭＬ－ＲＡＲα融合基因的表达不仅与ＡＰＬ的发病有关，而且钝化了维甲酸对ＡＰＬ细胞的诱导分化作用。     

维拉帕米联用阿霉素、长春新碱、足叶己甙体外净化白血病细胞

采用体外克隆形成培养技术研究维拉帕米（ＶＰＬ）联用阿霉素（ＡＤＭ）、长春新碱（ＶＣＲ）和足叶己甙（ＶＰ－１６）体外净化白血病细胞，结果显示：ＶＰＬ２．２μｍｏｌ／Ｌ能明显增加单一应用的不同浓度ＡＤＭ、ＶＣＲ或ＶＰ－１６对白血病细胞克隆形成单位（Ｌ－ＣＦＵ）的抑制作用，而不增加其对正常粒巨系克隆形成单位（ＧＭ－ＣＦＵ）的毒性。０．９２μｍｏｌ／ＬＡＤＭ、０．０２５０μｍｏｌ／ＬＶＣＲ及１．７０μｍｏｌ／ＬＶＰ－１６联合应用下，Ｌ－ＣＦＵ集落存活率为１１．６１％％，再联用２．２０μｍｏｌ／ＬＶＰＬ则为３．５２％；而相同条件下ＧＭ－ＣＦＵ集落存活率分别为３５．８１％及２６．０５％（与相应Ｌ－ＣＦＵ组相比，Ｐ＜０．０１），提示ＶＰＬ能明显增加上述化疗药物联合应用下的选择性体外净化白血病细胞作用。     

维拉帕米联用阿霉素,长春新碱,足叶己甙体外净化白血病细胞

采用体外克隆形成培养技术研究维拉帕米（ＶＰＬ）联用阿霉素（ＡＤＭ），长春新碱（ＶＣＲ）和足叶己甙（ＶＰ－１６）体外净化白血病细胞，结果显示，ＶＰＬ２．２μｍｏｌ／Ｌ能明显增加单一应用的不同浓度ＡＤＭ，ＶＣＲ或ＶＰ－１６对白血病细胞克隆形成单位（Ｌ－ＣＦＵ）的抑制作用，而不增加其对正常粒巨系克隆形成单位（ＧＭ－ＣＦＵ）的毒性，０．９２μｍｏｌ／ＬＡＤＭ，０．０２５０μｍｏｌ／ＬＶＣＲ及１．７０μｍｏ     

急性早幼粒细胞白血病PML-RARα融合基因异构体的临床意义

目的：探讨急性早幼粒细胞白血病（ＡＰＬ）ＰＭＬ－ＲＡＲα融合基因异构体及其临床关系。方法：用逆转录／聚合酶链反应（ＲＴ／ＰＣＲ）检测２１例初诊ＡＰＬ患者细胞中ＰＭＬ－ＲＡＲα融合基因转录本，并分析其对临床特征的可能不同影响。结果：在ＡＴＲＡ诱导治疗过程中，短型ＰＭＬ－ＲＡＲα异构体ＡＰＬ组的早期死亡和复发率（４／８）（５０％）高于长型ＰＭＬ－ＲＡＲα异构体组（２／１３）（１５．４％）；与长型异构体相反，存活期２月至２年患者含短型异构体的比例（７５％）大于存活期２年以上组（３３．３％）；未观察到患者血液学参数改变与不同ＰＭＬ－ＲＡＲα异构体有关。结论：与长型ＰＭＬ－ＲＡＲα异构体组ＡＰＬ患者相比，短型ＰＭＬ－ＲＡＲα异构体组的预后可能更差。     

磷脂酶A2激活倡导中性粒细胞凋亡

目的 探讨磷脂酶Ａ２（ＰＬＡ２）活性的变化对中性粒细胞凋亡的影响。方法 采用光镜、电镜、ＤＮＡ电泳和ＦＡＣＳ等方法，分析了磷脂酶Ａ２内外源性激活剂和掏剂对大鼠中性粒细胞凋亡影响。结果 脂多糖（ＬＰＳ１μｇ／ｍｌ）和钙离子载体（Ａ２３１８７１μｍｏｌ／Ｌ）等ＰＬＡ２激动剂分别在共同培养６ｈ和１２ｈ之内促进中性粒细胞凋亡，而ＰＬＡ２阻断剂４－溴苯甲酰溴甲烷（ｐ－ＢＰＢ０．０１～１ｍｍｏｌ／Ｌ）可明显     

流式细胞术三色分析循环活化血小板及其在缺血怀脑血管病中的 …

目的 探讨流式细胞术（ＦＣＭ）三色分析血液中循环活化血小板（ＣＡＰ）在缺血性脑血管病中的临床意义。方法 以纤维蛋白原受体（ＦＩＢ－Ｒ０、Ｐ－选择素（ＣＤ６２Ｐ）作为分子标志物,ＦＣＭ三色免疫荧光分析ＣＡＰ。结果 正常人静止血小板膜表面ＦＩＢ－Ｒ和ＣＤ６２Ｐ表达极低（阳性百分离的中位数分别为１．４％和０．７％）。０．１μｍｏｌ／Ｌ ＡＤＰ可使血小板明显活化,１０μｍｏｌ／Ｌ ＡＤＰ使活化血小板膜表面     

核黄素等抗甲基苄基亚硝胺的致细胞突变作用

采用ＣＨＯ细胞ＨＧＰＲＴ位点突变测定系统观察了核黄素、尼克酰胺和锌等微量营养素对甲基苄基亚硝胺（ＭＢＮＡ）致细胞突变作用的影响。结果表明，在ＲＰＭＩ１６４０培养基中添加核黄素（２．６～１３．０μｍｏｌ／Ｌ）或尼克酰胺（４０～１２０μｍｏｌ／Ｌ）可显著抑制ＭＢＮＡ的致细胞突变作用。联合应用核黄素、尼克酰胺和锌效果更佳。提示：核黄素等微量营养素可能具有阻止细胞癌变的作用，为应用微量营养素干预治疗癌变过程提供了一定的理论依据。     

呼吸中枢M受体亚型与细胞内钙

目的探讨呼吸中枢Ｍ受体亚型与细胞内钙的关系。方法采用Ｆｕｒａ－２双波长荧光法测定Ｍ１和Ｍ２受体激动剂和拮抗剂对胎鼠桥脑和延脑神经细胞内游离钙浓度（［Ｃａ２＋］ｉ）的影响。结果在静息状态下，其［Ｃａ２＋］ｉ为７２±６ｎｍｏｌ／Ｌ，加入２００μｍｏｌ／ＬＡｃｈ，［Ｃａ２＋］ｉ升高３８％，匹鲁卡品（Ｐｉｌ）０．４～２００μｍｏｌ／Ｌ无明显的升高细胞内钙的作用，而Ｍ２受体激动剂６β－乙酰氧基去甲托烷（６β－ＡＮ）１～１０μｍｏｌ／Ｌ。对神经细胞内的游离钙均无明显影响。Ｍ１－Ｒ拮抗剂哌仑西平（Ｐｉ）６０μｍｏｌ／Ｌ能拮抗Ａｃｈ升高细胞内钙的作用，Ｍ２－Ｒ拮抗剂ＡＦ－ＤＸ１１６２０μｍｏｌ／Ｌ不能拮抗。结论Ｍ１－Ｒ兴奋与细胞内钙升高有关。     

肝性脑病大鼠海马CA1区锥体细胞氨基酸激活电流改变

探讨氨基酸受体门控性离子通道电流改变在肝性脑病（ＨＥ）发生中的作用。方法用快速加药装置将５０μｍｏｌ／Ｌγ－氨基丁酸（ＧＡＢＡ）或１ｍｍｏｌ／ＬＮ－甲基－Ｄ－天冬氨酸（ＮＭＤＡ）＋１０μｍｏｌ／Ｌ甘氨酸（Ｇｌｙ）加于急性分离的大白鼠海马ＣＡ１区锥体细胞周围，用全细胞膜片钳技术记录ＧＡＢＡ或ＮＭＤＡ激活的电流。结果ＨＥ大鼠海马ＣＡ１区锥体细胞ＧＡＢＡ激活的内向电流的强度为４１３．３±４３６．８ｐＡ（３１例），比对照大鼠（２３３．４±１７９．７ｐＡ，２８例）有较大增强（ｔ＝２．１０４９，Ｐ＜０．０５）。并观察到ＨＥ大鼠ＧＡＢＡ激活电流的出现频率较对照大鼠高。ＨＥ大鼠ＮＭＤＡ激活的内向电流的强度为４０８．５±３１７．２ｐＡ（３７例），比对照大鼠（８８２．６±８９６．５ｐＡ，２６例）弱（ｔ＝２．５８５１，Ｐ＜０．０５）。两组大鼠ＮＭＤＡ激活电流的出现频率无明显差异。结论氨基酸受体门控性离子通道电流改变在肝性脑病发生中起一定作用。     

脑梗塞病人血浆LCAT活性与脂蛋白及红细胞膜脂质含量的关系

①目的探讨脑梗塞病人血浆卵磷脂－胆固醇酰基转移酶（ＬＣＡＴ）活性与脂蛋白及红细胞膜脂质含量的关系，②方法应用外加底物法铡定４８例脑梗塞病人和３６例健康人血浆ＬＣＡＴ括性，并检测血浆高密度脂蛋白胆固醇（ＨＤＬ－Ｃ）和亚组分（ＨＤＬ２－Ｃ，ＨＤＬ３－Ｃ）、低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）、载脂蛋白Ａ－１和Ｂ（ａｐｏＡ－１，ａｐｏＢ）、红细胞膜胆固醇（ＲＢＣＭ－ＣＨ）和红细胞膜磷腊（ＲＢＣＭ－ＰＬ）的含量变化。③结果脑梗塞病人血浆ＬＣＡＴ活性、ＨＤＬ－Ｃ，ＨＤＬ２－Ｃ及ａｐｏＡ－１含量明显降低，血浆ＬＤＬ－Ｃ，ａｐｏＢ，ＲＢＣＭ－ＣＨ及ＲＢＣＭ－ＣＨ／ＲＢＣＭ－ＰＬ比值显著增高，与对照组比较差异有显著性（ｔ＝２．１４－３．２５，Ｐ＜０．０５，０．０１），血浆ＬＣＡＴ活性与ＨＤＬ－Ｃ，ＨＤＬ２－Ｃ及ａｐｏＡ－１呈正相关（ｒ＝０．３８４，Ｐ＜０．０１；ｒ＝０．２９８，Ｐ＜０．０５；ｒ＝０．３１９，Ｐ＜０．０５），而与ＬＤＬ－Ｃ，ＲＢＣＭ－ＣＨ呈负相关（ｒ＝－０．２９０，－０．３２３；Ｐ＜０．０５）。④结论脑梗塞病人脂质代谢异常与血浆ＬＣＡＴ活性降低有关。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.