首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 62 毫秒
1.
KBV200细胞株耐药逆转前后HLA,B7抗原的表达   总被引:1,自引:0,他引:1  
张健  林学颜 《第一军医大学学报》2000,20(6):481-484,F002
目的 探讨肿瘤多药抗性细胞的免疫逃避机制。方法 利用特异性切割mdr1的核酶为工具,以表达Mdr1的耐药细胞株KBV2000为靶细胞。采用脂质体转染技术,将含核酶的质粒pH β Apr-Ineo/5m R3及空载体pH β Apr-Ineo导入KBV200及其亲本KB细胞内,运用Northem-blotting、免疫组化方法观察核酶对mdr1 mRNA及p-gp的影响,运用流式细胞仪技术检测各种不同细胞亚株HLA-Ⅰ、HLA-Ⅱ、B7-1、B7-2的表达。结果 含核酶的质粒pH β Apr-Ineo/5mR3及空载体pH β Apr-Ineo可以在KB、KBV200细胞中稳定表达,核酶可以特异性地切割mdr1,导致KVB200/5mR3的mdr1 mRNA含量下降,P糖蛋白(P-gp)表达减低,各种不同细胞亚株  相似文献   

2.
目的:评价mdr-1基因与P-gp糖蛋白在急性血病(AL)细胞中表达的相关性及其临床意义。方法:分别应用RT-PCR及流式细胞术(FCM)32例AL患者白血病细胞中的mdr-1 mRNA及P-gp糖蛋白表达率及表达水平,同时检测14例正常人作为对照。结果:临床非耐药组mdr-1阳性率为22.2%,P-gp阳性率为33.3%,而临床耐药组两者分别为78.6%,85.7%,均与临床治疗结果有极显著的一  相似文献   

3.
目的研究急性髓系白血病(AML)Hras/P21、抗凋亡基因(bcl2)、细胞凋亡与多药耐药基因(mdr1/Pgp)对疗效的影响,并证实流式细胞术(FCM)检测Pgp和逆转录多聚酶链反应(RTPCR)检测mdr1mRNA间的相关。方法用FCM检测85例初治AML患者的Hras/P21、bcl2、细胞凋亡和mdr1产物Pgp的表达;用RTPCR检测其中47例的mdr1/mRNA。结果P21+/Pgp+者完全缓解(CR)率明显低于P21-/Pgp-者(分别为58.1%和100%,P<0.025),高bcl2/Pgp+的CR率明显低于低bcl2/Pgp-者(分别为60%和96.3%,P<0.005)。Pgp+31例中29例mdr1mRNA阳性,Pgp-16例中仅2例mdr1mRNA阳性。结论Pgp+同时P21+、高bcl2者CR低,FCM检测Pgp和RTPCR检测mdr1mRNA相关性良好  相似文献   

4.
目的 研究急性髓系白血病(AML)H-ras/P21,抗凋亡基因、细胞凋亡与多药耐药基因的疗效的影响,并证实流式细胞术检测P-gp和逆转录多聚酶链反应(RT-PCR)检测mdr-1 mRNA间的相关。方法 用FCM检测85例初始AML患者的H-ras/P21、bcl-2,细胞凋亡和mdr-l产物P-gp的表达;用RT-PCR检测其中47例的mdr-l/mRNA。结果 P21^+/P-gp^+者完全  相似文献   

5.
目的采用一段人工合成的互补于人mdr1基因的反义硫代硫酸脱氧寡核苷酸(简称反义核酸)转染白血病多药耐药细胞株K562/ADM,来逆转白血病细胞的多药耐药。方法MTT法检测K562/ADM细胞对阿霉素的IC50,流式细胞仪分析细胞内的柔红霉素含量,免疫细胞化学染色方法确定P170的表达水平,RT-PCR检测mdr1-mRNA的相对水平(mdr1/β2-MG)。结果浓度为2μmol的反义核酸使K562/ADM细胞对阿霉素的IC50从处理前的25.72μg/ml降至处理后的6.97μg/ml,相对逆转效率为73.01%。反义核酸亦使K562/ADM细胞内的柔红霉素含量增加,P170表达阳性率从处理前的98.6%±1.0%降至处理后的18.3%±0.7%,mdr1/β2-MG下降了0.5,mdr1-mRNA相对水平有所下降。结论反义核酸通过下调mdr1基因,阻断P170的表达,从而降低K562/ADM的耐药性。  相似文献   

6.
目的 观察三苯氧胺(amoxifen,TAM)及雌激素(E2)对乳腺癌MCF-7细胞株耐药基因mdr-1mRNA水平的影响:方法 应用RT-PCR法观察TAM及E2在不同时间、浓度作用下细胞内mdr-1mRNA水平的变化;结果TAM可以使细胞内mdr-1mRNA水平降低,而E2则相反。且以上变化表现出剂量、时间依赖关系。结论 TAM通过降低mdr-1mRNA水平,使肿瘤细胞的耐药性降低,有利于抗肿  相似文献   

7.
异博定对乳腺癌细胞多药耐药性逆转作用的研究   总被引:5,自引:0,他引:5  
目的:检测异博定逆转乳腺癌耐药细胞株对阿霉素的耐药性。方法:MTT法检测细胞的抑制率变化,流式细胞仪检查异博定对MCF-7/Adr细胞的药物浓度变化。结果:异博定可提高肿瘤细胞的药物浓度(P<0.01),并且使MCF-7/Adr细胞对阿霉素的敏感性增加20倍。结论:本实验证实异博定可增加细胞内抗肿瘤药物浓度,逆转细胞的耐药性,为临床应用异博定逆转肿瘤的耐药性提供了理论基础。  相似文献   

8.
P—糖蛋白在头颈部恶性肿瘤中的表达及意义   总被引:4,自引:0,他引:4  
为探讨mdr1基因产物在头颈部恶性肿瘤中的表达状况及其与临床耐药性的关系,作者用免疫组化ABC法47例未化疗的头颈部恶性肿瘤进行P-糖蛋白表达的原位检测,同时应用细胞核仁组成区嗜银蛋白技术(AgNOR)测定了43例肿瘤对8种抗癌药物的敏感性。结果显示,头颈部恶中的P-糖蛋肿瘤中的P-糖蛋白表达阳性率为57%,肿瘤对VCR.ADM.PY<M,MMC4种药物的敏感率在P-糖蛋白阳性组明显低于阴性组,并有统计学意义。  相似文献   

9.
目的 通过对人肝癌细胞株(SMMC-7721)转染TGF-β1基因,观察该细胞MMP-2,9表达的变化。方法 用电穿孔法进行基因转染,并经Western blot,Northern blot法作鉴定,后分别应用Northern blot,Western blot法观察该细胞株表达MMP-2,9mRNA和MMP-2蛋白的变化。结果 经转染TGF-β1基因的细胞株MMP-2 mRNA和蛋白表达均高于对  相似文献   

10.
本文利用免疫荧光法对肿瘤细胞CEM,CEM/ADMP170-糖蛋白进行了检测,结果显示,敏感细胞全部阴性,而耐药细胞株P170-糖蛋白呈过度表达,其过度表达细胞随肿瘤细胞对抗癌药物耐受性增强而增加,其中CEM/ADM多耐药细胞株的P170-糖蛋白过度表达细胞为92%。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

15.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

16.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

17.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

18.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

19.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

20.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号