肺癌和肺结核患者外周血T淋巴细胞亚群的检测

目的　为了探讨T淋巴细胞亚群在肺癌和肺结核发生中的变化 ,采用流式细胞术检测肺癌 3 0例 ,肺结核 2 5例 ,正常人 2 0例的外周血T淋巴细胞亚群。结果　肺癌组和肺结核组CD3、CD4、CD4/CD8比值均明显低于正常组 (P <0 .0 1) ,而CD8则高于正常组 (P <0 .0 1) ,但两组各项指标无显著性差异 (P >0 .0 5 )。结论　肺癌及肺结核患者免疫功能低下 ,T淋巴细胞亚群的检测对监控患者的免疫状态 ,指导临床治疗 ,观察疗效及预后有关重要的意义     

食管、贲门癌患者术前术后细胞免疫变化及意义

目的 探讨食管癌贲门癌术前,术后免疫指标变化及其临床意义.方法 用流式细胞术定量检测55例患者外周血T淋巴细胞亚群,NK细胞及WBC术前,术后不同时期动态变化,并进行统计学分析.结果 (1)术后第8天患者T淋巴细胞亚群有所恢复,CD3、CD4、CD4/CD8增加,CD8减少,NK细胞增高(P<0.01);(2)术后2 d WBC值与术后8 d CD3、CD4、CD8值有相关性;(3)中晚期患者较早期患者CD3、CD4、CD4/CD8减少,CD8、NK增加(P<0.05);(4)有淋巴结转移患者较无淋巴结转移患者CD3、CD4、CD4/CD8减少,NK增加(P<0.05).结论 (1)术后患者免疫功能有所恢复.(2)术后炎症感染对患者免疫功能有刺激作用,可能为患者免疫功能增强起一定作用.(3)中晚期患者较早期患者免疫功能有明显下降.(4)有淋巴结转移患者较无淋巴结转移患者免疫功能有明下降.(5)手术是治疗食管癌贲门癌有效措施.     

恶性肿瘤患者T细胞亚群和可溶性白介素-2受体检测及意义

目的 :了解恶性肿瘤患者免疫功能的改变。方法 :对 2 0 0例恶性肿瘤患者及 30例正常人的T淋巴细胞亚群和可溶性白介素 - 2受体 (SIL - 2R)进行了同步检验及相关性研究。结果 :肿瘤患者CD3、CD4 、CD4 /CD8均低于正常人 (P <0 .0 5) ,SIL - 2R、CD8均高于正常人 (P <0 .0 5) ,其中CD4 /CD8与SIL - 2R呈负相关。结论 :恶性肿瘤患者的免疫功能是低下的。     

急性病毒性肝炎患者外周血T淋巴细胞亚群检测分析

目的 :了解急性甲、乙、丙型病毒性肝炎患者外周血T淋巴细胞亚群对疗效和预后判断的价值。方法 :用APAAP桥联酶标法检测 10 2例急性甲、乙、丙型病毒型肝炎患者外周血T淋巴细胞亚群CD3 、CD4、CD8、及CD4/CD8,并检测了 2 9例急性甲、乙、丙型病毒性肝炎患者治疗前后T淋巴细胞亚群变化。结果 :与正常对照相比 ,10 2例急性甲、乙、丙型病毒性肝炎患者外周血CD3 、CD4、CD4/CD8均有不同程度的降低 (P <0 . 0 5 ) ,而各型肝炎的CD8均显著增高 (P <0 . 0 1) ;2 9例患者治疗前后T淋巴细胞亚群比较 ,7例治疗效果差者 ,其CD4、CD4/CD8处于低水平而CD8始终处于高水平 (P >0 . 0 5 ) ,另 2 2例治疗效果较好者 ,各亚群指标均接近或恢复正常水平 (P <0 .0 5 )。结论 :急性甲、乙、丙型病毒性肝炎患者外周血T淋巴细胞亚群的改变是以CD4、CD4/CD8的降低 ,特别是CD8的显著增高为其特征 ,各亚群在治疗过程中的动态变化在一定程度上可能反映疾病的疗效及预后。治疗时 ,应注意调节机体的细胞免疫功能。     

鼻咽癌患者脾区低剂量照射对外周血T-淋巴细胞亚群的影响

目的 :探讨鼻咽癌 (NPC)患者脾区接受低剂量照射后外周血T 淋巴细胞亚群的变化。方法 :比较 5 8例常规放疗(NPC 1)前后和 5 0例放疗加脾区低剂量照射 (NPC 2 )前后淋巴细胞亚群的变化。T 淋巴细胞亚群用抗人T 淋巴细胞单克隆抗体致敏红细胞直接法检测。结果 :①NPC患者外周血T 淋巴细胞各亚群均低于正常人。常规放疗后的T 淋巴细胞较放疗前进一步下降 ,CD3 为 (48 31± 3 75 ) % ,CD4 为 (2 0 76± 4 70 ) % ,CD4 /CD8比值 0 95± 0 2 9,低于放疗前 ,(P <0 0 0 1)。但CD8无变化。② 5 0例NPC患者在放疗同时每周 1次脾区低剂量照射 ,放疗后的CD3 为 (5 2 6 2± 4 2 6 ) % ,CD4 为 (2 7 6 2±3 0 6 ) % ,高于放疗前 (P <0 0 0 1) ,CD8(19 14± 3 10 ) %低于放疗前 ;CD4 /CD8比值 1 48± 0 2 7,高于放疗前 (P <0 0 0 1)。结论 :NPC患者放疗期间接受低剂量的脾区放射 ,可刺激外周血T 淋巴细胞的CD3 和CD4 升高 ,抑制CD8。     

乙型肝炎肝硬化自发性细菌性腹膜炎患者T细胞亚群的研究

目的 :对 136例乙型肝炎肝硬化自发性细菌性腹膜炎患者的T淋巴细胞进行了研究 .方法 :采用间接荧光法检测了患者的T淋巴细胞亚群 (CD+ 3 ,CD+ 8和CD+ 4 /CD+ 8) .结果 :患者T淋巴细胞亚群表现为严重紊乱 ,与正常对照组比较 ,CD+ 3 T淋巴细胞明显下降 (P <0 0 1) ,CD+ 8T淋巴细胞明显升高 (P <0 0 1) ,导致CD+ 4 /CD+ 8明显降低 (P <0 0 1) .结论 :乙型肝炎肝硬化自发性细菌性腹膜炎患者的T淋巴细胞亚群紊乱 ,而使免疫功能紊乱 ,这对指导临床治疗和判断预后具有重要意义     

再生障碍性贫血患者T淋巴细胞亚群与免疫球蛋白的变化及其临床意义

目的　探讨再生障碍性贫血 (再障 )患者T淋巴细胞亚群与免疫球蛋白的变化及其临床意义。方法　对 2 2例再障患者 (再障组 )进行外周血T淋巴细胞亚群及其血清免疫球蛋白检测 ,并与 18例门诊健康体检者 (对照组 )进行比较。结果　再障组CD+ 4 、CD+ 4 /CD+ 8、IgG、IgA、IgM水平比对照组明显降低 (P均 <0 .0 1) ,但CD+ 8明显升高 (P <0 .0 1) ;大部分患者CD+ 3 正常。再障组治疗后 15例有效 ,其IgG、IgA、IgM、CD+ 4 、CD+ 4 /CD+ 8比值升高至正常或接近正常 ,CD+ 8明显降低至正常或接近正常 ,与治疗前相比差异有显著性 (P <0 .0 1或 0 .0 5 )。结论　再障患者发病与免疫功能异常密切相关 ,不仅存在细胞免疫功能的异常 ,也存在着体液免疫功能的异常。用免疫抑制剂治疗有效者 ,T淋巴细胞亚群、免疫球蛋白水平可恢复正常或接近正常     

大肠癌患者外周血T淋巴细胞亚群变化与病程的相关性

目的分析T淋巴细胞亚群变化在大肠癌患者不同病程中的相关性。方法检测58例不同时期大肠癌患者外周血T淋巴细胞亚群变化。结果A期大肠癌患者的CD3(T细胞总数)、CD4(辅助性T细胞)与正常对照组无显著差异(P>0.05),CD8(抑制性T细胞)明显低于正常对照组(P<0.05);B、C、D期患者CD8明显高于正常对照组(P<0.05,P<0.01),CD3、CD4、CD4/CD8明显低于正常对照组(P<0.01)。随着Dukes分期增加,CD3、CD4、CD4/CD8逐渐降低,CD8逐渐增高。结论大肠癌患者存在免疫功能低下,且T淋巴细胞亚群水平与大肠癌患者的病程有明显的相关性。     

非何杰金氏淋巴瘤患者外周血T淋巴细胞亚群测定分析

目的　测定中高度非何杰金氏淋巴瘤患者外周血T淋巴细胞亚群的数量。方法　采用酶免疫染色技术对 2 6例中高度非何杰金氏淋巴瘤患者外周血T淋巴细胞亚群进行测定并与 30例健康对照者进行比较。结果　患者组CD3下降 (P<0 0 1) ,CD4下降 (P <0 0 1) ,CD8升高 (P <0 0 1) ,CD4 /CD8下降 (P <0 0 1)。结论　中高度非何杰金氏淋巴瘤患者免疫失调 ,细胞免疫功能下降     

双黄连雾化吸入对毛细支气管炎患儿T细胞免疫功能作用的研究

目的 :探讨双黄连对毛细支气管炎患儿T淋巴细胞亚群的影响。方法 :采用APAAP桥联酶标法 ,对 3 5例毛细支气管炎患儿进行双黄连雾化吸入前后T淋巴细胞亚群检测 ,并与 3 0例健康儿相比较。结果 :毛细支气管炎患儿T淋巴细胞CD3、CD4、CD4/CD8比值明显低于健康儿童 (P <0 .0 1)。CD8值明显高于健康组儿童 (P <0 .0 5 )。毛细支气管炎患儿经双黄连治疗后较治疗前CD3、CD4、CD4/CD8比值明显回升 (P <0 .0 5 )。CD8明显回落 (P <0 .0 5 )。结论 :双黄连可调节人体T淋巴细胞亚群的平衡 ,起到加强患儿免疫功能的作用。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…