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1.
大黄药材HPLC指纹图谱的化学模式识别   总被引:6,自引:0,他引:6  
目的通过指纹图谱的相似度比较和化学模式识别,确定TY719注射液原料大黄的产地,并建立指纹图谱共有模式。方法应用中南大学梁逸曾教授的中药指纹图谱相似度计算软件,对不同产地的掌叶大黄药材的HPLC指纹图谱进行相似度比较和主成分分析。结果有4个样品有明显的差异性,主成分分析进一步证明了相似度比较的结果。而后对同一产地多批药材进行相似度比较和主成分分析,确立了大黄指纹图谱共有模式的方法,筛选出建立指纹图谱共有模式的10批大黄的药材。结论该方法简便,灵敏,可操作性强,为控制TY719注射液原料药材质量提供了有效的手段。可作为TY719注射液质量控制标准的判断方法。  相似文献   

2.
目的 建立银杏叶提取韧的毛细管电泳指纹图谱。方法 EGb761为对照,芦丁为内参比物,采用毛细管电泳法分析银杏黄酮苷。结果 利用指纹图谱相似度计算软件,计算出各厂家样品与EGb761的相似度指数,建立银沓叶提取物的指纹图谱.结论 该研究有助于银杏叶提取物的质量控制。  相似文献   

3.
地龙注射液HPLC指纹图谱的研究   总被引:3,自引:0,他引:3  
目的建立地龙注射液中游离氨基酸的HPLC指纹图谱。方法采用2,4-二硝基氟苯柱前衍生化法,用HPLC法进行梯度洗脱分析地龙注射液中游离的氨基酸。结果通过优化衍生条件,得到分离度、重复性均较好的HPLC指纹图谱,确定了14个共有峰,以缬氨酸为参照峰,用中药指纹图谱相似度软件计算不同批次样品的相似度均在0.97以上。结论本研究建立的指纹图谱,可用于地龙注射液的质量控制。  相似文献   

4.
目的建立岩黄连注射液的HPLC指纹图谱,用于控制产品的质量。方法采用HPLC法。Shim-pack CLC-ODS色谱柱(150mm×6.0mm,5μm),乙腈-缓冲溶液(1∶1)梯度洗脱,体积流量为1mL/min,检测波长285nm。中药色谱指纹图谱相似度评价系统的操作规范(版本2004A)计算。结果13批岩黄连注射液的相似度均在0.95以上,确定了12个共有峰,建立了该注射液HPLC指纹图谱。结论本法可作为岩黄连注射液质量控制方法。  相似文献   

5.
目的 建立茵栀黄注射液HPLC指纹图谱,以考察其一致性和稳定性,提高其质量控制水平.方法 采用高效液相色谱法,针对23批(包括不同厂家、不同批号、不同加速条件)茵栀黄注射液建立HPLC指纹图谱;利用相似度评价软件对指纹图谱进行相似度分析,并以相似度、共有峰相对峰面积为指标,进行样品聚类分析.结果 不同厂家的样品各自聚为一类,表明茵栀黄注射液各批次间产品质量具有稳定性;各厂家产品逐次聚为一类,表明各厂家产品具有差异性和相对一致性;同一批次不同稳定性加速试验样品与正常批次差异明显,表明指纹图谱检测具有灵敏性.结论 建立的HPLC指纹图谱可用于茵栀黄注射液质量控制,对保证产品质量的一致性和稳定性具有重要价值.  相似文献   

6.
白术中3种内酯成分的含量测定及指纹图谱研究   总被引:1,自引:0,他引:1       下载免费PDF全文
[目的]建立白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量测定方法和指纹图谱方法,结合系统聚类分析研究不同产地白术饮片的质量。[方法]建立高效液相色谱-紫外检测器(HPLC-UV)双波长测定白术内酯Ⅰ、Ⅱ、Ⅲ含量的方法和单波长测定指纹图谱的方法,对4个产地12批白术饮片进行了测定研究。[结果]白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量测定方法线性关系良好,加样回收率合格。不同产地白术中白术内酯成分含量相差在1.9~3.8倍之间,3种内酯成分总量差异最大为2.5倍,其中3种内酯成分总含量最高的为浙江白术。不同产地白术饮片指纹图谱相似度较高,相似度在0.972~0.998之间。系统聚类分析结果与含量测定和指纹图谱分析结果具有较好一致性。[结论]白术饮片中白术内酯的含量测定方法特征性强、操作简便,结合白术饮片指纹图谱与系统聚类分析可更好地控制白术的质量。  相似文献   

7.
目的:建立银杏达莫注射液原料、中间体和成品的指纹图谱,进行生产全程质量控制。方法:黄酮类成分采用Agela C18柱(4.6mm×150mm,5μm),乙腈-0.4%磷酸溶液(梯度)为流动相,流速1.0mL·min-1,检测波长360nm,柱温35℃。内酯类成分采用Agela C18柱(4.6mm×150mm,5μm),甲醇—四氢呋喃水(10:75)溶液(梯度)为流动相,流速0.8mL·min-1,用蒸发光散射检测器检测,柱温30℃。结果:银杏叶原药材、银杏叶提取物、银杏达莫注射液中间体及其银杏达莫注射液的指纹图谱有较好的相关性,本方法精密度、稳定性和重复性试验均符合《中药注射剂指纹图谱研究的技术要求(暂行)》中要求。结论:本研究建立的方法简单、重复性良好,为银杏叶原药材、银杏叶提取物、银杏达莫注射液中间体及银杏达莫注射液指纹图谱标准的确立提供更为科学的依据和有效的鉴别方法,有助于该制剂的质量控制。  相似文献   

8.
太子参HPLC指纹图谱的初步研究   总被引:5,自引:1,他引:5  
目的研究建立太子参的HPLC指纹图谱分析方法,提供药材质控指标。方法采用梯度洗脱的方法进行色谱分离,使用指纹图谱相似度评价软件进行数据处理,比较分析太子参指纹图谱的相似度。结果初步建立了江苏地产太子参的指纹图谱;不同产地太子参指纹图谱相似度较好。结论HPLC指纹图谱法重现性好,用于太子参的质量评价切实可行。  相似文献   

9.
目的建立益母草注射液的毛细管电泳指纹图谱。方法以水苏碱峰为参照物峰,采用毛细管区带电泳法建立益母草注射液指纹图谱。结果标示出益母草注射液中12个主要共有峰。结论所建立方法具有良好的重现性与分离度,可为益母草注射液的质量控制提供依据。  相似文献   

10.
目的建立卷丹的HPLC指纹图谱分析方法。方法取11份卷丹鳞叶样品,采用HPLC法,运用中药色谱指纹图谱相似度评价系统软件对数据进行分析。结果11批卷丹鳞叶中皂苷元提取物的HPLC指纹图谱相似度较好并有18个共有指纹峰,除6号样品外,其他样品的相似度均大于0.9。结论建立的HPLC指纹图谱模式准确可靠,可为卷丹药材质量评价及控制提供参考依据。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

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