液化石油气燃烧颗粒物对小鼠骨髓细胞微核率的影响

对液化石油气燃烧颗粒物诱发小鼠骨髓细胞微核进行了研究。结果表明，提取物剂量在１００～４００ｍｇ／ｋｇ之间，微核率由１０．９‰升至２８．８‰，呈明显的剂量－反应关系，各剂量组与对照组比较均具极显著性差异（Ｐ＜０．０１）。说明液化石油气燃烧颗粒物在体内可产生明显的遗传毒性作用，这种作用可能是该颗粒物诱发肺癌的机制之一。     

几味抗癌中药致突变性研究

目的：观察五加皮、茯苓、猪苓和山慈菇的遗传毒性。方法：应用活体小鼠骨髓嗜多染红细胞核试验（ＭＮＴ）和小鼠精子畸形试验。结果：五加皮各剂量所致的微核率和精子畸形率均低于正常对照组，分别为Ｐ＞００５，Ｐ＜００１。茯苓和猪苓各剂量所致的微核率和精子畸形率与正常对照组比较，无明显差异（Ｐ＞００５）。山慈菇诱发的微核率明显高于正常对照组（Ｐ＜００１）。结论：五加皮、茯苓和猪苓对体细胞及生殖细胞均无诱变损伤作用。山慈菇具有致突变性     

绞股蓝对环磷酰胺诱发小鼠骨髓细胞突变的抑制作用

目的观察干品绞股蓝对环磷酰胺（Ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ,以下简称ＣＰ）诱发小鼠骨髓嗜多染红细胞（ＰＣＥ）微核和染色体畸变的抑制作用．方法小鼠骨髓细胞微核试验方法和小鼠骨髓细胞染色体畸度试验方法．结果含１０％绞股蓝的饲料使环磷酰胺诱发的微核率从３３．１７±３．５４‰降至１２．００±２．１９‰（Ｐ＜０．００１）,染色体畸变率从２６．３３±３．５０％降至１４．６７±２．２５％（Ｐ＜０．０１）．结论绞股蓝可抑制环磷酰胺诱发的ＤＮＡ损伤,提示绞股蓝具有一定的抗诱变作用．     

抗氧化营养素对DNA损伤的保护作用

①目的探讨抗氧化营养素对ＤＮＡ氧化损伤的影响。②方法实验分为实验组和对照组，实验组（ｎ＝２５）连续服用β－胡萝卜素、维生素Ｅ和Ｃ（ＶｉｔＥ，ＶｉｔＣ）２８０ｄ，对照组（ｎ＝２５）未服药。两组均取全血分离淋巴细胞，应用单细胞琼脂电泳（ＳＣＧＥ）检测ＤＮＡ的自发损伤及经不同浓度Ｈ２Ｏ２（３０，１００，３００μｍｏｌ／Ｌ）处理后的ＤＮＡ损伤程度。③结果两组淋巴细胞自发性ＤＮＡ损伤率都很低，分别为１２．３和１２．１专用单位，两组比较无显著性差异（ｔ＝０．０６３，Ｐ＞０．０５）。经ＤＮＡ诱裂剂Ｈ２Ｏ２处理后，两组均随Ｈ２Ｏ２剂量的增加而呈现出ＤＮＡ损伤程度的逐渐加重，但在相同剂量条件下，实验组的ＤＮＡ损伤程度均明显低于对照组，均有显著性差异（ｔ＝２．３２０，Ｐ＜０，０５；ｔ＝３．９５０，Ｐ＜０．０１；ｔ＝５．５４９，Ｐ＜０．０１）。④结论抗氧化营养素有降低ＤＮＡ损伤的作用。     

低剂量γ射线与香烟对人外周血淋巴细胞微核率的影响

目的探讨低剂量γ－射线和香烟烟雾的诱变作用。方法取人外周血随机分成香烟烟雾组，γ射线组和香烟烟雾与γ射线联合组，进行微量全血培养，然后分别用不同剂量（０、０．２５、０．５、１．０Ｇｙ）的６０Ｃｏγ射线和不同浓度（０、０．２、０．４、０．６、０．８、１．０支）香烟烟雾进行处理，并检测和统计淋巴细胞微核。结果香烟烟雾（０．８、支）与低剂量γ射线（０．５、１．０Ｇｙ）均能提高淋巴细胞微核的形成（Ｐ＜０．０１），并具有剂量依赖效应；低剂量γ射线联合香烟烟雾处理淋巴细胞，诱发微核率显著高于对照组、单独照射组和香烟烟雾组（Ｐ＜０．０１）。结论低剂量γ射线联合香烟烟雾可加强遗传物质的损伤。     

屈指肌腱修复术后的动力牵引治疗

采用动力牵引治疗屈指肌腱修复术后患者２５例（实验组）。实验组术后第１天开始在保护下主动伸指被动屈指活动。结果：实验组的手功能理想恢复率为９２％，显著高于对照组（传统治疗组）的８％（Ｐ＜０．０１），粘连率为８％，显著低于对照组的７５％（Ｐ＜０．０１）。结果提示：该方法简单易行，疗效显著，适于临床推广应用。     

123例功能性消化不良的超声胃动力观察

观察功能性消化不良（ＦＤ）患者胃排空率及胃窦收缩幅度的情况。方法采用胃Ｂ超增效液定时观察１２３例ＦＤ患者和５９例健康志愿者。结论ＦＤ组３０ｍｉｎ、６０ｍｉｎ的排空率分别为（５１．２３±１５．２）％、（７９．９５±２２．８５）％,而５９例对照组的胃排空率分别为（７５．１６±１３．４）％、（９８．０７±５．４８）％,经统计学处理两组有非常显著性差异（Ｐ＜０．０１）。ＦＤ组胃窦收缩幅度为３．３１±１．３５,对照组为５．２５±１．９７,ＦＤ组收缩幅度明显小于对照组,在统计学上亦有明显差异（Ｐ＜０．０１）。结论胃动力障碍是ＦＤ发病的主要因素。     

1,6—二磷酸果糖抗失血性休克实验研究

本实验观察了ＦＤＰ抗失血性休克作用。３组失血性休克大鼠分别接受５％ＦＤＰ溶液（Ａ组）、５％葡萄糖溶液（Ｂ组）和生理盐水（Ｃ组）静注。给药６０ｍｉｎ后，Ａ组ＭＡＰ为１０．４±２．１ｋＰａ，与Ｂ、Ｃ组７．９±２．４和７．７±２．６ｋＰａ间均有差异（Ｐ＜０．０１）；Ａ组动物存活率７５％，而Ｂ、Ｃ组分别为２５％和１５％（Ｐ＜０．０１）；Ａ组动脉血ｐＨ和ＰａＯ２分别为７．２４±０．１４和１３．４６±１．２２ｋＰａ，与Ｂ、Ｃ组７．１４±０．１４和１０．２９±３．８４ｋＰａ与７．０２±０．１０和７．２５±２．１２ｋＰａ间有显著差异（Ｐ＜０．０１）；Ａ组回输血液再灌流后血浆ＳＯＤ和ＭＤＡ分别为１２００±１２６Ｕｍｌ－１和０．４７±０．２５ｎｍｏｌｍｌ－１，同Ｂ、Ｃ组１０８１±１１９Ｕｍｌ－１和０．９１±０．２９ｎｍｏｌ－１与８３５±９Ｕｍｌ－１和１．１３±０．１６ｎｍｏｌ－１间均有显著差异（Ｐ＜０．０１）。结果表明ＦＤＰ保护组织损伤而产生抗休克作用。     

不同病因腹泻病儿肠道主要菌群变化的研究和比较

对８８例不同病因腹泻儿肠道双歧杆菌、乳酸杆菌、大肠杆菌的变化进行研究。对照组２５例。结果：研究组较对照组双歧杆菌、乳酸杆菌有显著下降（Ｐ＜０．０１和Ｐ＜０．０５），大肠杆菌下降不明显（Ｐ＜０．０５）。非感染性腹泻组较感染性腹泻组双歧杆菌、乳酸杆菌下降更显著（Ｐ＜０．０１和Ｐ＜０．０５），大肠杆菌下降不显著（Ｐ＜０．０５）。感染性腹泻组中，双歧杆菌在轮状病毒肠炎组中较急性菌痢下降更显著（Ｐ＜０．０５），而其它两种菌下降程度相差不明显（两者Ｐ＜０．０５）     

147Pm诱导血红细胞系对60Coγ射线辐射的适应性反应

目的　研究低剂量１４７Ｐｍ 内照射能否改变相继高剂量６０Ｃｏγ射线引起的ＲＢＣ 损伤的适应性效应。方法　应用外周血ＲＢＣ计数、网织红细胞（ＲＣ）计数、正常染红细胞（ＮＣＥ） 微核和嗜多染红细胞（ＰＣＥ） 微核检测,观察低剂量１４７Ｐｍ 内污染诱导小鼠ＲＢＣ系统的适应性反应。结果　剂量为２ ．０Ｇｙ 的６０Ｃｏ γ射线外照射可引起明显的血ＲＢＣ 系统损伤,与正常对照组比较,ＲＢＣ和ＲＣ计数显著降低（ Ｐ＜ ０．０１） ;ＮＣＥ和ＰＣＥ微核细胞率明显增高（Ｐ＜ ０．０１） 。预先用０．３７、３ ．７ 和３７Ｂｑ／ｇ 体重的１４７Ｐｍ 内污染预处理动物,３ 天后再予２．０Ｇｙ 的高剂量６０Ｃｏ γ射线外照射,ＲＢＣ和ＲＣ计数则显著增高,ＮＣＥ和ＰＣＥ微核细胞率显著降低,与单纯高剂量６０Ｃｏ γ射线外照射组比较有非常显著性差异（Ｐ＜ ０．０１）;并且某些指标与正常对照组比较已无显著性差异（ Ｐ＞ ０．０５） 。结论　预先用低剂量１４７Ｐｍ β射线内照射动物, 可诱导血ＲＢＣ 系统的放射适应性反应,使血ＲＢＣ系统对相继高剂量６０Ｃｏ γ射线外照射引起的损伤效应有了明显的抵抗力。提示外周血ＲＢＣ 系统可作为低剂量辐射细胞生物效应的观察指标之一     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…