辅酶Q10对UVB损伤人角质形成细胞的保护作用

目的　探讨辅酶Q10对中波紫外线(UVB)照射下人角质形成细胞氧化损伤的保护作用。方法　原代培养人角质形成细胞,建立UVB辐照人角质形成细胞氧化损伤模型,MTT法测定细胞活性;流式细胞仪测定细胞增殖指数;酶法测定脂质过氧化产物丙二醛(MDA)的水平及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH -Px)等抗氧化物酶活性。结果　应用辅酶Q10后,受损细胞的活性及增殖指数均显著增高,细胞上清液中SOD ,GSH -Px的活性增高,丙二醛(MDA)含量降低,且呈量效正比关系。结论　辅酶Q10有对抗UVB氧化损伤,提高受损细胞的活性及增殖指数作用。其保护机制可能是通过清除氧自由基,提高抗氧化物酶活性来发挥的。     

葛根素对中波紫外线损伤HaCaT细胞的抗氧化作用

目的研究葛根素不同处理方式对其抗氧化能力的影响。方法以30mJ/cm2中波紫外线(UVB)和1.0mg/mL葛根素处理HaCaT细胞。实验分为阴性对照组、模型组、UVB照射前给药组、UVB照射后给药组和UVB照射前后给药组。UVB照射后24h收集细胞,检测细胞内活性氧(ROS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPH-Px)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)和丙二醛(MDA)。结果 UVB照射前给药处理和UVB照射前后给药处理,使细胞内T-AOC、CAT、GSH-Px升高,而ROS和MDA降低(P<0.05);UVB照射后给药处理可以升高SOD(P<0.05),但对其他指标无影响(P>0.05)。结论葛根素不同处理方式影响其抗氧化能力,其中预防性结合治疗性处理效果最佳,单纯预防性处理其次,单纯治疗性处理最差。     

泥鳅多糖对UVB诱导HaCaT细胞光损伤的保护作用

目的:探讨泥鳅多糖对UVB诱导的人角质形成细胞(HaCaT)光损伤的保护作用。方法:用不同剂量(10 mJ·cm~(-2)、20 mJ·cm~(-2)、30 mJ·cm~(-2)、40 mJ·cm~(-2)、50 mJ·cm~(-2)、60 mJ·cm~(-2))的UVB照射细胞,选择合适的UVB照射剂量建立HaCaT细胞的光损伤模型。用不同质量浓度(10 mg·L~(-1)、20 mg·L~(-1)、40 mg·L~(-1)、80 mg·L~(-1)、100 mg·L~(-1))的泥鳅多糖作用于正常的HaCaT细胞,筛选泥鳅多糖的给药浓度。经60 mJ·cm~(-2)剂量UVB照射HaCaT细胞后,给予浓度为10 mg·L~(-1)的泥鳅多糖处理,用MTT法检测细胞活性,用分光光度法检测细胞内超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)水平。结果:经60 mJ·cm~(-2)剂量UVB照射后,与对照组比较,模型组HaCaT细胞活性显著下降,SOD活性显著下降,MDA水平显著升高。40 mg·L~(-1)以上的泥鳅多糖对细胞增殖有显著的抑制作用,选择10 mg·L~(-1)的泥鳅多糖作为给药浓度,与模型组比较,泥鳅多糖处理能显著提高细胞活性,提高SOD活性,降低MDA水平,显著减轻了细胞的光损伤程度,差异均有统计学意义(P0.05)。结论:泥鳅多糖能够通过提高细胞内SOD活性,加速清除活性氧,抑制膜脂过氧化,增强细胞的抗氧化能力,对UVB诱导的HaCaT细胞光损伤有显著的保护作用。     

红景天苷对乙醇诱导L-02肝细胞氧化损伤的保护作用

目的:探讨红景天苷对乙醇诱导的人L-02肝细胞氧化损伤的保护作用。方法:体外检测不同浓度梯度的红景天苷对人L-02肝细胞活性的影响;建立体外乙醇处理诱导的氧化损伤细胞模型,并以不同浓度红景天苷进行处理24h,检测肝细胞MDA水平、SOD和GSH-Px的活性。结果:与正常对照组相比,乙醇模型组MDA水平显著上升,SOD、CAT和GSH-Px的活性明显下降(P<0.05),经红景天苷处理,MDA水平下降,SOD和GSH-Px的活性活力显著上升(P<0.05)。结论:红景天苷可以减轻脂质过氧化程度,降低MDA含量,并提高肝细胞抗氧化的能力,使SOD和GSH-Px的活性升高,发挥了对肝细胞的保护作用。     

人参三醇组甙、VitE及VitC 对紫外辐射致皮肤损伤的防护机制初探

目的:探讨紫外辐射致皮肤损伤的药物防护机制.方法:以细胞凋亡、细胞周期及细胞内SOD、H2O2为观察指标,研究人参三醇组甙及VitE、VitC对UVB致人角质形成细胞(Colo16)损伤的保护作用.结果:一定剂量UVB可诱导明显的皮肤角质形成细胞发生S期阻滞和细胞凋亡,可使细胞内SOD下降、H2O2升高,人参三醇组甙及VitE、VitC对其均有不同程度的保护作用:减少S期细胞数、抑制细胞凋亡、激活细胞内SOD活性、降低细胞内H2O2.结论:紫外线照射后细胞内氧自由基变化可能是紫外线致皮肤损伤的机理之一.     

参芩仿生化提取物对中波紫外线照射致生物体损伤的保护作用

目的通过人参、黄芩仿生化提取物,对中波紫外线(UVB)照射致人永生化角质形成细胞(HaCaT)及小鼠皮肤损伤的保护作用进行研究,为中药抗辐射提供理论依据。方法采用MTT法检测HaCaT细胞生存率,黄嘌呤氧化酶法检测细胞溶解液中及小鼠皮肤组织匀浆中超氧化物歧化酶(SOD)活性,硫代巴比妥酸法检测细胞溶解液中及小鼠皮肤组织匀浆中丙二醛(MDA)含量变化。结果参芩仿生化提取物能够显著提高UVB照射损伤细胞的活性,明显升高细胞溶解液及小鼠皮肤组织匀浆中SOD活力,降低MDA含量。结论参芩仿生化提取物对UVB辐射致HaCaT细胞和小鼠皮肤损伤均有明显的保护作用,能够有效的抵抗UVB所致的氧化损伤。     

枸杞多糖在UVB辐射人角质形成细胞氧化损伤中的保护作用

目的利用中波紫外线辐射体外培养的人角质形成细胞（HaCaT）建立紫外线损伤模型,探讨枸杞多糖（LBP）在紫外线辐射HaCaT氧化损伤中的作用及其机制,为抗氧化剂的研发提供理论依据。方法在HaCaT培养的基础上设置空白对照组、UVB照射组和不同浓度的LBP干预组,采用UVB辐射HaCaT进行造模,运用酶生化法检测不同浓度的枸杞多糖对UVB辐射HaCaT后的细胞增殖及抗氧化酶的影响。结果 UVB辐射对HaCaT造成明显损伤,LBP可提高UVB辐射后HaCaT细胞增值活性（MTT）,提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性,降低丙二醛（MDA）产生。（P〈0.05）。结论 UVB对HaCaT细胞有损伤作用,LBP可拮抗UVB所致HaCaT细胞抗氧化酶活性的降低,从而具有抗氧化光保护作用。     

UVA对人角质形成细胞的氧化损伤作用

①目的　探讨紫外线A(UVA)对人角质形成细胞氧化损伤的机制。②方法　用 5J/cm2 UVA照射角质形成细胞 ,酶生化法检测活性氧 (ROS)的含量及超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH PX)活性的变化 ,流式细胞仪测定紫外线对角质形成细胞凋亡的影响 ,原位杂交技术观察 p2 1mRNA的变化 ,并与非照射组比较。③结果　与对照组比较 ,UVA照射组ROS含量升高 (t =113.6 8,P <0 .0 0 1) ,SOD、GSH PX活性下降 (t =5 7.2 3、19.0 4 ,P <0 .0 0 1) ,细胞凋亡率升高 (t=5 3.2 8,P <0 .0 0 1) ,p2 1mRNA表达增强。④结论　UVA对人角质形成细胞的损伤与氧自由基生成增多及细胞抗氧化能力抑制有关     

芦荟多糖对UVB辐射人角质形成细胞氧化损伤的保护作用

目的利用中波紫外线辐射体外培养人永生化角质形成细胞株（HaCaT细胞株）建立紫外线损伤人表皮细胞的模型,探讨芦荟多糖（AP）在紫外辐射HaCaT细胞氧化损伤中的保护作用及其机制。方法在HaCaT细胞培养的基础上,采用UVB辐射HaCaT后,立即加入AP进行干预,同时设置空白组与UVB对照组,分别运用MTT法及生化比色法检测不同浓度的芦荟多糖对UVB辐射人角质形成细胞增殖及抗氧化酶活性的影响。结果 UVB辐射对HaCaT造成明显损伤,AP可提高UVB辐射后HaCaT细胞增值活性,提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性,降低脂质过氧化物酶（MDA）活性（P〈0.05）。结论 UVB对HaCaT细胞有损伤作用,AP可拮抗UVB所致HaCaT细胞抗氧化酶活性的降低,具有光保护作用。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

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