乙型肝炎病毒前C区基因变异与T细胞免疫状态的研究

目的:探讨乙型肝炎病毒(HBV)前C区基因变异与T细胞免疫状态的关系。方法:通过DNA扩增、基因序列分析检测21例慢性乙型肝炎的基因序列,通过流式细胞分析技术(FCM)检测外周血T细胞亚群。结果:45例慢性乙肝发生前C变异有12例,变异株CD4+、CD8+相对数明显低于野生株组,差异有统计学意义(P<0.05)。结论:慢性乙肝前C变异后病毒得以逃避机体的免疫攻击,不利于宿主对病毒的清除     

熔点曲线法研究HBV基因B型BCP区变异与临床表现的关系

目的 研究乙型肝炎病毒(HBV)基因B型基本核心启动子(BCP)的变异及其临床意义.方法 收集HBV携带者、慢性乙型肝炎、慢性重型肝炎等3种临床类型的患者血清226份,采用巢式多聚酶链式反应(PCR)扩增HBV DNA,选取上述3种类型各50例B基因型患者,采用熔点曲线方法进行BCP区的变异检测.结果 226例患者中B基因型210例(92.9％),C基因型16例(7.1％)；150例不同病情的B基因型患者中,慢性重型肝炎患者的熔点曲线平均波峰数量多于慢性HBV携带者及慢性乙型肝炎患者,差异有统计学意义(P＜0.05),而HBV携带者与慢性乙型肝炎比较,差异无统计学意义(P＞0.05).结论 B基因型患者BCP区变异与临床表现存在一定的关系,病情重者准种数量较多.     

慢性乙型肝炎患者IFN治疗与HBV DNA前C区变异关系的研究

目的：通过检测慢性乙型肝炎患者干扰素（IFN）治疗前后HBVDNA前C区1896位变异,探讨其与IFN治疗之间的关系。方法：采用PCR—RFLP法检测慢性乙型肝炎患者前C区1896位变异,观察IFN治疗前后变异株的变化及IFN疗效。结果：200例慢性乙型肝炎患者IFN治疗前变异株检出率为19％,IFN治疗后未检出新出现的变异株;变异株、野毒株感染的慢性乙型肝炎患者IFN治疗有效率分别为60％与50％,均显著高于对照组（P=0．007,P〈0．001）,变异株、野毒株感染的慢性乙型肝炎患者IFN治疗有效率比较无差异（P〉0．05）。结论：IFN治疗无诱导前C区1896位变异的作用;干扰素治疗变异株病毒感染的慢性乙型肝炎有效,其近期疗效与野毒株感染的慢性乙型肝炎基本相同。     

乙肝病毒前C/C区基因一级结构及变异特征的临床探讨

目的:探讨乙型肝炎病毒(HBV)前C/C基因突变与HBV-DNA水平及转氨酶的关系;方法:收集慢性乙型病毒性肝炎患者血清423份,进行前C/C区基因测序、HBV标志物、HBV-DNA、ALT、AST测定.结果:HBVnt1896突变毒株158例,nt1899突变毒株57例.C区变异大部分集中在第5、27、60位氨基酸的...     

变异株与野毒株慢性乙型肝炎干扰素治疗疗效比较

干扰素(IFN-α)是慢性乙型肝炎抗病毒治疗的有效药物,但关于IFN治疗由变异株病毒或野毒株病毒感染的慢性乙型肝炎的疗效何者更优尚无定论。本文根据轻度慢性乙型肝炎患者前C区1896位的变异情况分为变异株组和野毒株组,比较两组采用IFN治疗的临床疗效。1资料与方法1.1临床资料19     

重型肝炎感染模式的实验研究

目的：探讨重型肝炎病原学感染模式,及临床类型与病原学关系。方法：采用ELISA、fWR等方法检测98例重型肝炎的血清肝炎病毒标志物。结果：表明急性重型肝炎发生率较低（1．0％）,以甲、乙型肝炎病毒感染为主,亚急性重型肝炎和慢性重型肝炎占重型肝炎的97％,以乙型肝炎病毒为主,病毒之间的重＊感染促使重型肝炎的发展。结论：研究提示乙型肝炎病毒仍是引起重型肝炎的主要病毒。     

慢性重型肝炎患者乙肝病毒C基因变异的初步研究

目的探讨乙肝病毒C基因变异与乙型肝炎患者病情的关系.方法用聚合酶链反应(PCR)对2份慢性重型乙型肝炎患者血清及1份乙肝病毒无症状携带者血清进行C基因扩增和克隆,经核苷酸序列分析,与我国HBV克隆株pADR1比较.结果乙肝病毒无症状携带者的C基因与pADR-1的基因相似;而慢重肝患者C基因变异率较高,各有19和14个核苷酸变异.结论乙肝病毒C基因的变异可能与重型肝炎的发生密切相关.     

慢性肝病患者乙型肝炎病毒核心基因启动子变异与临床类型的相关性研究

目的:研究慢性肝病患者乙型肝炎病毒核心基因启动子(HBV BCP)变异与临床类型的相关关系.方法:采用PCR微板核酸杂交结合ELISA检测显示技术,对176例HBV慢性感染患者的血清进行HBV BCP区核苷酸(nt)1762碱基A→T和1764G→A联合突变检测.根据病情的严重程度将患者分为6组不同临床类型:①慢性乙型肝炎轻度;②慢性乙型肝炎中度;③慢性乙型肝炎重度;④肝炎肝硬化;⑤慢性重型肝炎;⑥原发性肝癌.并与HBV BCP变异的阳性或阴性进行等级相关分析.结果:HBV BCP双变异在慢性乙型肝炎轻、中、重度组,肝炎肝硬化组,慢性重症肝炎组和原发性肝癌组的阳性率分别为28.0%、31.4%、34.2%、39.4%、60.0%和70.0%(rs=0.259,P=0.001).结论:HBV BCP变异与HBV慢性感染的由轻至重的临床类型呈正相关,随着病情的加重,BCP变异的阳性率亦随之增高.     

恩替卡韦在慢性乙型重型肝炎中的应用

乙型肝炎病毒感染是慢性重型肝炎发病机制中的重要因素之一,乙型肝炎病毒在肝细胞内持续复制,机体细胞免疫反应增强,诱导机体免疫功能损伤和肝细胞大量坏死,导致肝功能衰竭。恩替卡韦是目前抗乙型肝炎病毒中起效最快的一种核苷类药物,快速有效的降低慢性乙型重型肝炎患者血清及肝组织内的病毒载量,减少肝细胞表面的靶抗原,有助于阻断病情进展。     

HBV C基因启动子变异及基因型与肝硬化及原发性肝癌的相关性研究

目的 探讨乙型肝炎病毒C基因启动子(CP)和前C区基因变异及HBV基因型与肝硬化及原发性肝癌(HCC)的关系.方法 通过DNA扩增、基因序列分析检测44例慢性乙型肝炎(CH),39例活动性肝硬化(LC),34例HCC患者血清HBV S基因序列,确定其基因型,测定HBV CP和前C区序列,确定其变异状况.结果 HCC、LC患者CP双变异(nt 1762A→T和1764G→A)发生率(82.4%、71.8%)显著高于CH患者(45.5%)(P＜0.01、P＜0.01);HCC、LC患者C基因型检出率(70.6%、69.2%)显著高于CH患者(43.2%)(P值均＜0.05).结论 乙型肝炎病毒CP双变异、C基因型与HCC、LC密切相关.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.