RP-HPLC测定头孢克肟胶囊含量及有关物质

目的　探讨建立测定头孢克肟胶囊的含量及其有关物质的反相高效液相色谱分析方法。方法　HPLC色谱系统为C18柱 (2 5 0mm× 4 .6mm ,5 μm) ;流动相 :以乙腈 / pH7.0四丁基氢氧化铵溶液 (取 0 .4mol/L四丁基氢氧化铵溶液 2 5ml,用水稀释至 10 0 0ml,用 1.5mol/L的磷酸调节pH值至 7.0 ) (2 2 5 / 775 ,v/v) ;检测波长 :2 5 4mm ;流速 :1.0ml/min ;室温操作。结果头孢克肟在浓度为 175 5 .5～ 87.8μg/L范围内 ,与峰面积呈良好的线性关系 (r =0 .9999)。平均回收率为 99.4 9% ,RSD为 0 .4 0 %。测得的 3个批号胶囊的头孢克肟含量分别为 97.39%、97.96 %、98.13% ;其有关物质含量分别为 0 .6 2 %、0 .5 2 %、0 .95 %。结论　本法试剂易得 ,样品处理简便 ,分析快速准确 ,重现性好 ,可以作为头孢克肟胶囊剂的含量及有关物质测定方法。     

高效液相色谱法测定头孢克肟胶囊含量

目的 :建立高效液相色谱法测定头孢克肟胶囊的含量。方法 :HPLC色谱系统为Phenomenexprodigy ODS柱 (2 5 0mm× 4 6mm ,5 μm) ;流动相 :0 2 5 %四丁基氢氧化铵溶液 (1 5mol·L- 1 磷酸调pH至 7 0 )∶乙腈 (2∶1 ,v v) ;检测波长 :2 88nm ,流速 :1 2ml·min- 1 ;温度 :30°C。结果 :头孢克肟在浓度为 2 0～ 4 0 3μg·ml- 1 范围内 ,与峰面积呈良好的线性关系 (r =0 99998)。平均回收率为 1 0 3 82 % ,RSD为 1 1 6 %。结论 :本法简便可行 ,快速准确。可以作为头孢克肟胶囊剂的含量测定方法     

头孢克肟栓剂的制备及质量评价

[目的]制备头孢克肟栓剂并评价其质量.[方法]热熔法制备头孢克肟栓剂,采用RP - HPLC法,色谱柱为HYPERSIL C18柱(4.6 mm×250 mm,5μm),用乙腈:0.25％四丁基氢氧化铵溶液(1:2)为流动相,检测波长为288nm处测定自制头孢克肟栓剂的含量和体外溶出度.[结果]制备的头孢克肟栓剂质量稳...     

高效液相色谱法测定头孢克肟咀嚼片的含量

目的建立头孢克肟咀嚼片高效液相色谱法含量测定方法。方法色谱柱为Eclipse XDB—C18柱（150mm×4．6mm,5μm）;流动相为四丁基氢氧化胺（取10％四丁基氢氧化铵溶液10mL,加水690mL,摇匀,用1．5mol·L^-1磷酸调节pH至7．0）．乙腈（70：30）;流速为08mL·min^-1;检测波长为288nm。结果头孢克肟咀嚼片的浓度在0．025mg·mL^-1-1．6mg·mL^-1范围内线性关系良好,回归方程Y=66902X＋1926．6,相关系数r=0．9993;精密度试验RSD％=0．1％;平均回收率为100．9％,RSD％=0．56％。结论本方法简便、准确、重复性好,适用于头孢克肟咀嚼片的含量测定。     

色谱分析中样品浓度对保留时间的影响

目的：采用反相高效液相色谱法（RP-HPLC法）测定头孢克肟,研究样品浓度对头孢克肟保留时间的影响。方法：采用WatersSymmetry-C18色谱柱（4.6mm×150mm,5μm）;流动相：水-乙腈[73.5∶26.5（10%四丁基氢氧化铵25ml加水至1000ml,用85%磷酸调节pH至7.0）];检测波长：254nm;流速：1.0ml/min。结果：头孢克肟浓度为0.005mg/ml时平均保留时间为12.325min;浓度为0.500mg/ml时平均保留时间为11.895min,平均差为0.428min,经统计学处理,有显著性差异（P〈0.01）。结论：实验结果表明,头孢克肟浓度相差100倍时,保留时间相差3.47%,即：当头孢克肟浓度达到一定高度时,保留时间会随着浓度的增加而缩短,初步研究显示这种现象具有普遍性。     

HPLC法流动相选择及相浓度比例对头孢克肟分散片含量测定分析

目的:建立高效液相色谱法测定头孢克肟分散片的含量及有关物质的方法。方法:色谱柱:LunaC18(250mm×4.6mm,5μm),四丁基氢氧化铵溶液∶乙腈(70∶30)为流动相,检测波长为254nm。流速1.0mL/min,进样量:10μL,柱温:40℃。结果:头孢克肟在进样量25～300mg/L的范围内,线性关系良好;回归方程:Y=3.65×104X-1.25×104,r=0.9998。有关物质在进样量150～1500mg/L的范围内线性关系良好;回归方程:Y=578×103X-36×103,r=0.9998。结论:本法试剂易得,样品处理简便,分析快速准确,重现性好,可以作为头孢克肟分散片的含量及有关物质测定方法。     

HPLC法测定力蜚能胶囊含量及含量均匀度

目的建立在酸性条件下 ,力蜚能胶囊中含铁量的测定方法。方法HPLC法 ,以NuoleosilODS柱 (2 5 0mm× 4 .6mm ,10μm)为固定相 ,流动相为乙腈 -四丁基氢氧化铵溶液 (6∶94 ) ,流速为 1.5mL/min ,检测波长为 30 0nm。 结果线性范围为 19.77～ 98.89μg/mL ,回收率为 99.4 % ,RSD =1.5 %。 结论本法结果准确 ,重复性良好 ,方法简便。     

头孢克肟相关物质的RP-HPLC方法建立分析

目的对采用RP-HPLC法对头孢克肟相关物质进行检测分析的具体情况,为今后实验室工作提供参考.方法色谱条件:C18柱,规格250mm×4.6mm,10um;流动相为:PH值为7.0,浓度为0.1mol/L的醋酸铵缓冲液-乙腈(96︰4),检测波长为254nm,流速为1.0mL/min,柱温为35℃.在以上条件下对头孢克肟相关物质进行测定.结果头孢克肟浓度在1754.9-88.3ug/L范围内时存在良好线性关系,r=0.9999,10小时内稳定性号良好,重现性良好.结论采取RP-HPLC法检测头孢克肟相关物质含量时具有分析速度快,重现性好,能够将其作为对头孢克肟的含量以及相关物质的测定方法.     

HPLC法测定盐酸特比萘芬阴道用凝胶的含量

目的建立用HPLC法测定盐酸特比萘芬阴道用凝胶中盐酸特比萘芬含量的方法。方法Hypersil ODS2柱;检测波长:280nm;流动相:四氢呋喃-乙腈-四甲基氢氧化铵缓冲液(取10%四甲基氢氧化铵14.5mL,加水800mL,用0.7mol/L磷酸溶液调pH值至7.8,加水稀释至1000mL)=15∶60∶25;柱温:25℃;流速:1.0mL/m in;进样量:20μL。结果线性范围为121.08μg/mL-282.52μg/mL(r=0.9998,n=5);精密度试验的RSD为0.35%;回收率为100.3%,RSD为0.31%;测定3批自制样品含量均在90.0%-110.0%之间。结论本法简单、快速、精确、重复性高,可作为产品的质量控制方法。     

HPLC测定复方头孢克洛胶囊含量、含量均匀度及溶出度的研究

目的:建立测定复方头孢克洛胶囊含量、含量均匀度及溶出度的高效液相色谱法。方法:采用Waters Shield RP C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.025 mol/L磷酸二氢钾溶液(用磷酸调节pH值至3.0)(22:78)为流动相,流速1.0 mL/min,柱温30℃,检测波长249 nm。结果:含量、含量均匀度中头孢克洛和盐酸溴己新的线性范围分别为280.97～842.90μg/mL(r=1.0000)和11.00～33.01μg/mL(r=1.0000);复方头孢克洛胶囊中头孢克洛和溴己新平均回收率(n=3)分别为99.8%、100.3%(RSD为0.25%、0.86%)和101.2%、100.4%(RSD为0.47%、0.98%);溶出度中头孢克洛和盐酸溴己新的线性范围分别为141.71～472.38μg/mL(r=0.9991)和4.83～14.53μg/mL(r=0.9991);复方头孢克洛胶囊中头孢克洛和溴己新平均回收率(n=3)分别为99.6%、100.2%(RSD为0.22%、0.64%)和100.8%、99.9%(RSD为0.72%、0.47%)。样品检测结果显示,复方头孢克洛胶囊中头孢克洛和溴己新含量均匀度均良好,胶囊在30 min取样,头孢克洛溶出量达到90%以上,溴己新溶出量达到70%以上。结论:建立的HPLC法具有专属性强、灵敏度高、操作简便等特点,可同时考察头孢克洛和盐酸溴己新含量、含量均匀度及溶出度,可进一步有效控制药品质量。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…