D-101型大孔树脂残留物的顶空进样法分析研究

目的：建立中药用D－101型大孔树脂后有机溶剂残留物的检测方法。方法：采用顶空气相色谱法，对正己烷、苯、甲苯、对二甲苯、邻二甲苯、苯乙烯及二乙烯苯进行检测。结果：方法的精密度RSD均小于8．0％，峰面积与浓度均有良好的线性关系，回收率均符合要求。结论：该方法操作简便、准确可靠，可控制中药用D－101型大孔树脂后有机溶剂残留量。     

顶空气相色谱法检测茵栀黄提取物中苯系列残留物

<正> 茵栀黄提取物是由茵陈、栀子和大黄提取精制而成,是治疗黄疸性肝炎有效药物的原料药。其使用了D-101型大孔树脂迸行分离纯化,由于D-101型大孔树脂中可能带来苯、甲苯、对二甲苯、邻二甲苯、苯乙烯等残留物,因此,有必要对茵栀黄提取物中的苯系列残留物进行检测,对其残留量加以控制。本文采用毛细管气相色谱法,配以顶空进样,同时对5种苯系列残留物进行测定,操作简便,重现性好,结果准确可靠。     

何首乌中二苯乙烯苷与总蒽醌的提取与分离

目的：HPD500型大孔树脂提取二苯乙烯苷、D101型大孔树脂提取总蒽醌的浓度,同时比较D101型大孔树脂和乙醚萃取法分离出总蒽醌的纯度。方法：溶剂回流提取法初提,大孔树脂吸附法提纯。总蒽醌的提纯中同时使用吸附法和萃取法,比较其提取含量高低。结果：HPD500型大孔树脂提取二苯乙烯苷浓度为64.76%,D101型大孔树脂提取的总蒽醌为8.22%,乙醚萃取总蒽醌含量为7.55%。结论：HPD500型大孔树脂对二苯乙烯苷吸附能力强,且纯度又高,D101型大孔树脂对总蒽醌吸附能力强,优于乙醚萃取法,且毒性较小。     

大孔吸附树脂纯化丹参总酚酸的工艺研究

目的:利用大孔吸附树脂纯化丹参总酚酸,提高制剂中生药含量。方法:采用酸碱浸泡的方式对大孔吸附树脂进行前处理;采用气相色谱法对树脂洗脱液中的残留物进行检测;采用显色反应对树脂吸附力进行判定及对洗脱条件进行优选。结果:基本确定D101型大孔树脂富集、纯化丹参总酚酸工艺条件与参数。结论:大孔吸附树脂纯化丹参总酚酸的工艺可行,能够使丹参总酚酸的含量达到50%以上。     

独一味滴丸中大孔吸附树脂残留物限量检查研究

独一味滴丸是由中药独一味经提取、加工而制成的单味中药口服制剂,具有活血止痛,化瘀止血之功效[1]。在其制备工艺过程中采用了AB8型大孔吸附树脂,该树脂的残留物和裂解产物等对人体都有不同程度的伤害,为了保证用药安全,对其残留物和裂解产物进行了限量检查。本研究采用毛细管气相色谱法测定[2],配以顶空进样[3],同时对AB8型大孔吸附树脂可能带来的6种苯系列残留物即苯、甲苯、对二甲苯、邻二甲苯、苯乙烯、二乙烯苯进行检测,     

大孔吸附树脂对桃叶珊瑚苷分离效果的研究

目的：优选分离车前草伞草中桃叶珊瑚苷的大孔树脂。方法：将车前草粗提液置大孔树脂叶中吸附，并以水、50％和70％乙醇分别洗脱，用紫外分光光度法测定各洗脱液中桃叶珊瑚苷含量。结果：D100、D101、D140、D141、D19和D301R6种型号大孔树脂效果等片较大。结论：D141和D301R型大孔树脂能较好的富集桃叶珊瑚苷。     

顶空毛细管气相色谱法测定银杏叶片中大孔树脂残留物

目的：建立银杏叶片中9 种大孔树脂有机溶剂残留物的检测方法。方法：采用顶空气相色谱法测定大孔树脂残留物正己烷、苯、甲苯、邻二甲苯、间二甲苯、对二甲苯、苯乙烯、二乙基苯和二乙烯苯的含量。结果：9种大孔树脂残留物得到较好的分离,呈良好线性关系（r为0.999 2-0.999 8）,精密度良好,平均回收率为80%-110%。结论：该方法准确快捷,灵敏度高,适用于银杏叶片中树脂残留物的检查。     

顶空气相色谱检测甘草苷中大孔吸附树脂残留物

目的:建立顶空气相色谱测定甘草苷中大孔吸附树脂有机残留物的含量。方法:采用HP-INNOWAX石英毛细管柱(0.32 mm×30 m,0.25μm);以N,N-二甲基甲酰胺为溶剂,对正己烷、丙酮、苯、甲苯、二甲苯、苯乙烯等残留物进行检测。结果:被测物质均能得到很好的分离,RSD均<3.0%,峰面积与浓度线性关系良好,回收率为93.2%~100.7%。结论:该方法稳定、准确,适用于应用D-101型大孔吸附树脂纯化后原料药中有机溶剂残留物的检测。     

大孔树脂使用次数对羟基红花黄色素A 吸附率的影响

目的:考察D101大孔吸附树脂使用次数对羟基红花黄色素A吸附率的影响,为产业化生产提供依据。方法:采用静态吸附法,对反复再生后的D101大孔吸附树脂衰减规律进行了研究,并采用高效液相色谱法对羟基红花黄色素A含量进行了定量分析。结果:D101大孔吸附树脂在经过乙醇、2%盐酸、5%氢氧化钠反复再生后,使用16次吸附率下降约27%。结论:D101大孔吸附树脂在活化再生使用16次以后,不宜再用。     

大孔吸附树脂分离纯化黄芪总皂苷的研究

目的：研究黄芪总皂苷的纯化方法。方法：黄芪水提醇沉的浓缩液，通过经处理的D101大孔吸附树脂，用不同浓度的乙醇进行梯度洗脱，UV法跟踪检测。结果：70％乙醇洗液中黄芪总皂苷的含量最高，可达到70％以上。结论：D101大孔吸附树脂可有效地分离提纯黄芪总皂苷。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.     

瑞香科五属植物中酚性聚合物的结构特征、分布及药理活性研究进展

瑞香科植物广泛分布于世界热带和温带地区,其中多种植物可供药用。在对该科植物的研究中发现,黄酮类、木脂素和香豆素等酚性化合物的低聚体为其重要的特征性成分,药理活性主要表现在抗肿瘤、抗炎、抗氧化、抗菌和抗病毒等方面。对已发现的酚性聚合体的化学结构、植物来源及药理活性进行综述,为瑞香科植物今后的研究和开发利用提供参考。     

HPLC法同时测定沉香化滞丸中11种成分

目的采用HPLC梯度洗脱法同时测定沉香化滞丸中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚11种成分。方法采用Thermo Syncronis C18色谱柱(250 mm×4.6 mm,5μm),流动相为水-乙腈,梯度洗脱:0～10 min,20%乙腈;10～20 min,20%～40%乙腈;20～24 min,40%乙腈;24～26 min,40%～52%乙腈;26～30 min,52%乙腈;30～31 min,52%～90%乙腈;31～35 min,90%乙腈;35～40 min,90%～100%乙腈;40～43min,100%乙腈;43～45min,100%～20%乙腈;检测波长215nm,体积流量1.0m L/min,柱温30℃,进样量20μL。结果各成分在43 min内分离良好,沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚的线性范围分别为1.4～13.6、10.0～200.0、31.5～315.0、1.0～120.1、1.8～50.6、0.93～10.1、1.8～30.0、0.2～40.3、1.8～18.1、1.7～25.0、0.45～10.70μg/mL;样品中各成分的平均回收率均在98.90%～100.87%;11种成分精密度RSD在0.55%～1.54%;供试品溶液在30 h内稳定性良好,RSD在0.75%～1.94%;重复性RSD在0.39%～1.73%。6批次样品中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚质量分数分别为92.0～201.0、511.5～9 033.0、5 475.0～12 635.5、54.5～5 095.5、192.0～2 137.5、117.0～391.5、106.5～1 281.5、13.0～136.5、93.5～199.0、177.0～1 207.0、33.5～251.5μg/g。结论本方法准确、快速、简便,重复性好,精密度高,适用于沉香化滞丸中多种活性成分的定量分析。