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1.
李江  谢鸣 《北京中医药》2008,27(11):888-890
目的 观察小柴胡汤采用不同的提取方法 获取的提取物对小鼠H22肝癌实体瘤及免疫器官的影响,优选小柴胡汤抗肝肿瘤的最佳提取方法 .方法 建立小鼠H22肝癌模型,分别灌服不同提取方法 获取的小柴胡汤提取物(13.5 g生药/kg),腹腔注射5-氟尿嘧啶(5-FU)(20 mg/kg)做阳性对照;给药10天后检测各组的瘤重、体重、脾脏指数和胸腺指数,优选出最佳小柴胡汤提取方法 .结果 小柴胡汤水提组、梯度乙醇组及醇水组对H22小鼠肝癌实体瘤均有明显抑制作用,抑瘤率分别为32.35%、43.97%和25.77%,最佳提取方法 为梯度乙醇组.与正常组比较,H22荷瘤小鼠脾脏指数升高(P<0.01),胸腺指数和体重变化不大(P>0.05),小柴胡汤各提取工艺组脾脏指数均明显升高(P<0.01),5-FU组胸腺指数极显著降低(P<0.001).小柴胡汤各组小鼠体重均无明显影响,而5-FU组小鼠体重下降明显(P<0.001).结论 小柴胡汤采用水煎法、梯度乙醇提取法及醇水法获得的提取物对小鼠H22肝癌实体瘤生长有明显抑制作用,优选出的最佳提取方法 为梯度乙醇提取法.  相似文献   

2.
目的观察小柴胡汤采用不同的提取方法获取的提取物对小鼠H22肝癌实体瘤及免疫器官的影响,优选小柴胡汤抗肝肿瘤的最佳提取方法。方法建立小鼠H22肝癌模型,分别灌服不同提取方法获取的小柴胡汤提取物(13.5 g生药/kg),腹腔注射5-氟尿嘧啶(5-FU)(20 mg/kg)做阳性对照;给药10天后检测各组的瘤重、体重、脾脏指数和胸腺指数,优选出最佳小柴胡汤提取方法。结果小柴胡汤水提组、梯度乙醇组及醇水组对H22小鼠肝癌实体瘤均有明显抑制作用,抑瘤率分别为32.35%、43.97%和25.77%,最佳提取方法为梯度乙醇组。与正常组比较,H22荷瘤小鼠脾脏指数升高(P<0.01),胸腺指数和体重变化不大(P>0.05),小柴胡汤各提取工艺组脾脏指数均明显升高(P<0.01),5-FU组胸腺指数极显著降低(P<0.001)。小柴胡汤各组小鼠体重均无明显影响,而5-FU组小鼠体重下降明显(P<0.001)。结论小柴胡汤采用水煎法、梯度乙醇提取法及醇水法获得的提取物对小鼠H22肝癌实体瘤生长有明显抑制作用,优选出的最佳提取方法为梯度乙醇提取法。  相似文献   

3.
林燕  李兴广 《世界中医药》2009,4(6):304-306
脑-肠互动在胃肠疾病发病机制中起重要作用,肝郁脾虚是涉及肝脾二脏最常见的中医病机和临床证候,二者分属中西医完全不同的医学体系,但在本质上是高度一致的。本文以肠易激综合征为研究对象,从脑-肠互动角度探讨了肝郁脾虚的病理实质。中医学肝失疏泄,横逆犯脾,脾气渐虚,肝脾不调的病机观点和现代医学脑-肠轴学说及脑-肠互动机制之间可能存在密切关联,两者间可能具有某些共同的、客观的、量化的物质基础。  相似文献   

4.
李晋  徐尚福  罗果  白国辉 《中成药》2014,(11):2238-2242
目的观察小柴胡汤对肝纤维化大鼠肝组织细胞外信号调节激酶2(ERK2)、磷酸肌醇-3激酶(PI-3K)表达的影响,探讨小柴胡汤治疗肝纤维化的分子机制。方法皮下注射10%四氯化碳制备大鼠肝纤维化模型,第9周开始四氯化碳给药同时给予小柴胡汤灌胃治疗6周。通过血清生化检测反映肝脏功能;HE染色法观察肝组织病理学变化;免疫组织化学方法观察肝组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达,以反映肝星状细胞的活化情况;Real time RT-PCR观察ERK2、PI-3K mRNA的表达。结果与模型组相比,小柴胡汤中、高剂量组在血清学和组织学上均有明显改善,肝组织ERK2、PI-3K mRNA的表达明显减少,具有统计学意义。结论小柴胡汤抗四氯化碳诱导大鼠肝纤维化作用可能与干预Ras/ERK和PI-3K信号通路有关。  相似文献   

5.
心理应激(压力)正逐渐成为影响人类身心健康的重要因素,也是临床慢性难治性疾病进展的重要因素之一。心理应激反应可归属于中医情志病范畴,肝主疏泄,调畅情志,相关学者认为中医肝主疏泄是心理应激反应的核心。慢性或反复的心理应激,机体可由最初的抑郁、焦虑情绪行为的改变,逐渐出现胃肠功能不适,这与情志不遂-肝郁气滞-乘克脾土-肝郁脾虚(肝脾不调)形成过程相似,肝郁脾虚亦为心理应激相关性疾病常见中医证候,具有疏肝健脾功效的逍遥散已成为抗应激的经典方药。现代研究表明,肠道微生物不但参与消化道的运动、结构与功能,还可通过脑-肠轴影响宿主的大脑功能与行为,微生物-脑-肠轴已成为心理应激引发机体出现胃肠道症状和大脑行为异常的重要环节。笔者结合心理应激,探讨了肝郁脾虚证与微生物-脑-肠轴的相关性及逍遥散的干预机制,以丰富中医肝郁脾虚证候科学内涵。  相似文献   

6.
肝郁脾虚证是临床中常见的一种证候类型,既有肝郁情志不舒的表现,又有脾虚消化功能不好的表现。而脑-肠肽不仅对胃肠道有调控作用,还可以参与情绪的调节,这与肝郁脾虚证发生的机制颇为符合。目前发现的脑-肠肽有10多种,本文就其中几种与肝郁脾虚证最为密切的脑-肠肽进行展开综述,来探讨脑-肠肽与肝郁脾虚证之间的关系。  相似文献   

7.
目的观察胰高血糖素样肽-2(GLP-2)对小鼠小肠黏膜的作用效果;检测胰高血糖素样肽-2受体(GLP-2R)在小鼠不同脏器的分布和表达。方法选用5~6周龄的雄性健康BALB/C小鼠,体质量17~20 g。随机分为3组,腹腔注射组9只,脑内注射组9只,对照组8只,分别给予相应处理,3 d后处死小鼠,做组织切片进行HE染色,观察小鼠小肠黏膜的组织学变化,用免疫组化方法检测GLP-2R在不同脏器的表达和分布。结果①小鼠经腹腔注射GLP-2后,小肠不同肠段黏膜的绒毛高度较对照组明显增加(P均0.05);脑内注射组的肠黏膜无明显形态学变化。②GLP-2R在小鼠胃、空肠、结肠、气管等部位均有表达,而食管、肝、肺、肾与膀胱显阴性。③腹腔注射组的GLP-2R表达较对照组显著下调(P0.05),而脑内注射组的GLP-2R表达无变化。结论 GLP-2能刺激小肠黏膜上皮增厚,增加小肠不同肠段黏膜的绒毛高度;小鼠的胃、空肠、结肠、气管均有GLP-2R的表达,食管、肝、肺、肾与膀胱无GLP-2R的表达。  相似文献   

8.
小柴胡汤及其药群配伍抗小鼠H22肝肿瘤及免疫调节作用   总被引:2,自引:2,他引:2  
李江  谢鸣  甘媛 《中国中药杂志》2008,33(9):1039-1039
目的:观察小柴胡汤及其药群配伍对小鼠H22肝癌实体瘤的抑制作用及对荷瘤小鼠免疫功能的影响,研究小柴胡汤抗肝肿瘤的作用、量效关系及作用机制,探讨小柴胡汤及药群内在配伍意义。方法:建立小鼠H22肝癌模型,分别灌服高、中、低剂量的小柴胡汤煎剂(27,13.5,6.75 g.kg-1),腹腔注射5-氟尿嘧啶(5-FU)(20mg.kg-1)做阳性对照;给药10 d后检测各组的瘤重、体重、脾脏和胸腺指数。进一步观察有效剂量(13.5 g.kg-1)小柴胡汤药群配伍煎剂(柴-芩、姜-夏、参-枣-草、柴-芩+姜-夏、柴-芩+参-枣-草、姜-夏+参-枣-草,全方)对小鼠H22肝癌模型的上述作用。在此基础上,观察小柴胡汤及其有效药群(参-枣-草)对该荷瘤小鼠自然杀伤(NK)细胞、T淋巴细胞增殖及白细胞介素-2(IL-2)的影响。结果:小柴胡汤高、中、低3个剂量组对小鼠H22肝癌实体瘤均有明显抑制作用,抑瘤率分别为49.66%,48.52%,36.91%,药群配伍研究中参-枣-草组和全方组的抑瘤率为41.55%和43.65%。与正常组比,H22荷瘤小鼠脾脏指数显著升高(P<0.001),小柴胡汤高、中、低剂量组和各药群配伍组脾脏指数均明显升高(P<0.01);胸腺指数除全方组外,不同药群配伍组与正常组比均有显著降低(P<0.05),5-FU组有极显著降低(P<0.001);小柴胡汤各组小鼠体重均无明显变化,而5-Fu组小鼠体重下降明显(P<0.001)。与模型组比,小柴胡汤组小鼠NK细胞、T淋巴细胞增殖及IL-2活性均明显升高(P<0.001);参-枣-草组NK细胞和IL-2活性均升高(P<0.05);5-FU组NK细胞、T淋巴细胞增殖及IL-2活性均显著降低(P<0.01)。结论:小柴胡汤及其方中参-枣-草配伍药群有抑制小鼠H22肝癌实体瘤生长和提高荷瘤宿主免疫功能作用,其抑瘤作用机制与5-氟尿嘧啶的直接抑瘤有所不同,可能与增强荷瘤宿主免疫功能有关,提示该方中的扶正作用药群(人参、大枣和甘草)可能是其抑瘤作用的核心药群。  相似文献   

9.
目的:观察健脾调肝汤治疗肠易激综合征腹泻型肝郁脾虚证的临床疗效。方法:治疗组用健脾调肝汤,对照组口服马来酸曲美布丁100mg,每天3次,饭前30分钟口服,谷维素20mg每天3次。结果:治疗组在总疗效和中医症状总积分的改善上较对照组明显,二者具有显著差异(P0.05)。结论:健脾调肝汤治疗肠易激综合征腹泻型肝郁脾虚证具有显著疗效。  相似文献   

10.
目的通过观察脾虚肝癌小鼠的各组织有机阴离子转运肽2b1表达的情况探讨脾虚肝癌"因湿致瘀"的机制。方法C57BL/6小鼠随机分为空白组、脾虚组、肝癌组和脾虚肝癌组。观察肝癌小鼠的体质量和瘤体大小,并取各组小鼠肺、胃、肝、肾、脾、结肠、小肠和肝癌组织,检测各组织中Oatp2b1表达情况。结果脾虚肝癌组与肝癌组小鼠的体质量和瘤体体积出现差异(P0.05)。Oatp2b1 mRNA在脾虚肝癌小鼠的肺、脾、结肠和小肠组织表达量较肝癌组升高(P0.05),在癌组织中表达量也明显升高(P0.05),而肝组织中表达却明显降低(P0.05)。结论 Oatp2b1在脾虚肝癌小鼠肝组织的低表达和癌组织高表达,可能是脾虚肝癌"因湿致瘀"的分子机制之一。  相似文献   

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12.
目的:研究草苁蓉水萃取物(BRAF)对四氯化碳(CCl4)诱发的急性肝损伤小鼠肝脏氧化应激的干预作用.方法:将实验小鼠随机分为对照组、模型组、水飞蓟素阳性对照组(50 mg· kg-1)及BRAF高、低剂量组(200,100 mg·kg-1).每日给小鼠灌胃水飞蓟素或BRAF 1次,共7d.实验末期,腹腔注射CCl4建立小鼠急性肝损伤模型,苏木素-伊红(HE)染色法观察肝组织病理学变化,比色法检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性及肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、Na+-K+-ATP酶(Na+-K+-ATPase)、Ca2+-Mg2+-ATP酶(Ca2+-Mg2+-ATPase)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)含量.结果:BRAF明显降低CCl4致急性肝损伤小鼠血清ALT,AST和ALP水平,减轻肝组织损伤,升高肝脏SOD,CAT,GPx和GSH水平,升高肝线粒体SOD,Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性,降低肝匀浆及线粒体MDA含量.结论:BRAF降低CCl4致急性肝损伤小鼠肝脏氧化应激,对CCl4致小鼠急性肝损伤具有保护作用.  相似文献   

13.
江颖倩  彭梦超  吴建国  吴岩斌  吴锦忠 《中草药》2021,52(19):5932-5938
目的研究长片金线兰多糖的结构特征以及对四氯化碳(CCl_4)致小鼠急性肝损伤的保护作用。方法从长片金线兰中提取多糖,分析其相对分子质量、单糖组成、紫外与红外吸收特征。将48只ICR小鼠随机分为对照组、模型组、联苯双酯(150 mg/kg)组和长片金线兰多糖低、中、高剂量(100、200、400 mg/kg)组,给予相应药物干预9 d后,除对照组外,其余各组ip CCl_4造成急性肝损伤,16 h后检测各组小鼠血清中丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)和乳酸脱氢酶(lactate dehydrogenase,LDH)活性;测定各组大鼠肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性以及谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量;采用苏木素-伊红(HE)染色法观察各组小鼠肝脏组织病理变化。结果长片金线兰多糖的相对分子质量为4.254×10~5,由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖组成(物质的量比为1∶0.11∶0.19∶11.15∶0.87∶0.36∶0.18),紫外与红外光谱扫描结果推测其可能为一种吡喃型杂多糖。与模型组比较,长片金线兰多糖能够显著降低CCl_4致肝损伤小鼠血清中ALT、AST和LDH活性(P0.05、0.01),提高肝脏组织中SOD、CAT活性和GSH含量(P0.05、0.01),降低MDA含量(P0.01),减轻CCl_4对肝组织的病理损伤。结论长片金线兰多糖对CCl_4致小鼠急性肝损伤具有一定的保护作用,其机制可能与抑制脂质过氧化有关。  相似文献   

14.

Background/aims

Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe.

Methods

Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1—concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2—CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3—CCl4 alone for 8 weeks; group 4—TCM 319 recipe only for 8 weeks; group 5—untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rβ, and transforming growth factor-beta 1 (TGF-β1) were measured by quantitative RT-PCR and western blot.

Results

TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rβ, and it also suppressed protein expression of PDGF-Rβ and TGF-β1.

Conclusions

TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rβ, and with the suppression of protein expression of PDGF-Rβ and TGF-β1.  相似文献   

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16.

Aim of this study

Graptopetalum paraguayense E. Walther, a vegetable consumed in Taiwan, has been used in folk medicine for protection against liver injury, although its actual efficacy remains uncertain. Therefore, we investigated the protective effects of Graptopetalum paraguayense E. Walther against carbon tetrachloride (CCl4)-induced liver damage in rats.

Materials and methods

Water extracts of Graptopetalum paraguayense E. Walther (WGP) were administered for 8 consecutive weeks to male Sprague-Dawley rats. And a dose-dependent manner in preventing liver damage was confirmed. Moreover, the major ingredient of WGP, gallic acid, was also orally administrated in the CCl4-induced rats. The hepatoprotective activity was assessed using various biochemical parameters such as antioxidant enzymes and histopathological studies.

Results

WGP ranging from 50 to 300 mg/kg bw administrations significantly lowered serum aspartate transaminase (AST) and alanine transaminase (ALT) levels, and inhibited malondialdehyde (MDA) generation in CCl4-treated rats. WGP increased cellular GSH level and antioxidant enzymes, including superoxide dismutase, glutathione reductase, and catalase. Serum tumor necrosis factor-alpha (TNF-α) was decreased in the group treated with CCl4 plus WGP (150 and 300 mg/kg bw). Histopathological examination of livers showed that WGP reduced fatty degeneration, cytoplasmic vacuolization and necrosis in CCl4-treated rats. In contrary, 10 mg/kg bw of gallic acid was administrated, this dose was related with WGP (300 mg/kg bw), and had significantly decreased the AST and ALT compared to the CCl4-treated group. Aforesaid results suggested that gallic acid from WGP offered antioxidative activity against CCl4-induced oxidative liver damage.

Conclusions

Taken together, this study is the first time to suggest that Graptopetalum paraguayense E. Walther exerts hepatoprotection via promoting antioxidative and anti-inflammatory properties against CCl4-induced oxidative liver damage.  相似文献   

17.

Aim of the study

Coptidis rhizoma (CR, Chinese name is Huanglian) has been used in treating infectious and inflammatory diseases for two thousand years in Traditional Chinese Medicine (TCM). Its related pharmacological basis for the therapeutics has been studied intensively, but CR can also be used for vomiting of “dampness-heat type or acid regurgitation” due to “liver-fire attacking stomach” in TCM, whose symptoms seem to link the hepatic and biliary disorders, yet details in the therapies of liver diseases and underlying mechanism(s) remain unclear. To clarify this ethnopharmacological relevance, hepatoprotective effect of Coptidis rhizoma aqueous extract (CRAE) and its possible mechanism were studied in rats intoxicated with carbon tetrachloride (CCl4) in the present study.

Materials and methods

Sprague–Dawley (SD) rats aged 7 weeks old were intraperitoneally injected with CCl4 at a dose of 1.0 ml/kg as a 50% olive oil solution. The rats were orally given the CRAE at doses of 400, 600, 800 mg/kg and 120 mg/kg berberine body weight (BW) after 6 h of CCl4 treatment. At 24 h after CCl4 injection, samples of blood and liver were collected and then biochemical parameters and histological studies were carried out.

Results

The results showed that CRAE and berberine inhibited significantly the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and increased the activity of superoxide dismutase (SOD). Observation on the hepatoprotective effect of berberine was consistent to that of CRAE.

Conclusion

The study is the first time to demonstrate that CRAE has hepatoprotective effect on acute liver injuries induced by CCl4, and the results suggest that the effect of CRAE against CCl4-induced liver damage is related to antioxidant property.  相似文献   

18.
目的:研究忍冬叶总黄酮(TFLLJ)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分为正常组、模型组、TFLLJ高、中、低剂量组(400,200,100 mg.kg-1)和联苯双酯组(150 mg.kg-1),除正常组和模型组外,其余各组小鼠每日ig给药1次,共10 d。末次给药1 h后,除正常组外其余各组ip 0.1%CCl4花生油溶液(0.01 mL.g-1)1次,造成急性肝损伤。测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)含量,计算肝脏指数(LI),测定肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,观察肝组织病理改变。结果:TFLLJ(400,200,100 mg.kg-1)剂量组可降低CCl4致小鼠肝损伤血清中的ALT和AST(P<0.01或P<0.05)。可降低小鼠肝组织中MDA和SOD活性(P<0.01或P<0.05)。TFLLJ各剂量组能明显改善肝组织的病理变化。结论:忍冬叶总黄酮对CCl4致小鼠急性肝损伤具有一定的保护作用,可能与抗脂质氧化有关。  相似文献   

19.
北柴胡正丁醇部位保肝作用及其化学成分特征初步研究   总被引:1,自引:1,他引:0  
目的:研究北柴胡正丁醇萃取部位对小鼠急性肝损伤的保护作用并对其化学成分特征进行初步研究.方法:采用腹腔注射四氯化碳诱导小鼠急性肝损伤,以柴胡正丁醇萃取部位高、中、低剂量(20,10,4 g·kg-1)同时预防给药7d,检测空白组、模型组、对照组、不同剂量柴胡正丁醇萃取部位组小鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)活性,同时测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;建立并分析北柴胡正丁醇萃取部位HPLC色谱图.结果:北柴胡正丁醇萃取部位是以柴胡皂苷a和柴胡皂苷d为主要成分的柴胡总皂苷,可有效抑制造模小鼠血清中ALT,AST的升高(P<0.05),同时可提高造模小鼠肝组织中SOD的活力,降低肝组织中MDA的含量(P<0.05).结论:北柴胡正丁醇萃取部位对于小鼠急性肝损伤具有保护作用,其化学成分以柴胡皂苷类为主.  相似文献   

20.
叶下珠抗肝细胞损伤作用机制   总被引:11,自引:0,他引:11  
叶下珠能明显降低CCl4引起的小鼠血清ALT的升高,抑制肝脏MDA的生成。叶下珠体外与大鼠肝细胞共同孵育,能抑制CCl4所致肝细胞膜流动性降低,降低〔Ca2+〕i浓度。结果提示,叶下珠保护肝脏损伤作用可能与其抗指质过氧化和膜保护作用有关。  相似文献   

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