Sedative and hypnotic effect of freeze-dried paeoniflorin and Sini San freeze-dried powder in pentobarbital sodium-induced mice

OBJECTIVE： To investigate the sedative and hyp- notic activity of paeoniflorin and freeze-dried Sini San powder on mice and provide a reliable method for determining the pharmacodynamic material ba- sis of Sini San. METHODS： Male adult mice weighing 20-22 g were used in this study. Three experiments were carried out. Synergism with pentobarbital was used as an index for hypnotic effect. Loss of the righting reflex was used to determine the start of sleep. Sleep latency and sleeping time were recorded in each experiment. RESULTS： The coefficient of variation of the suprathreshold dose （55 mg/kg） was significantly lower than that of the threshold dose. The sleep latency of mice was significantly decreased, and the sleep- ing time of mice was significantly prolonged. The effects of paeoniflorin and Sini San on prolonging the sleeping time of mice induced by pentobarbital sodium were significantly stronger than those in the control group. CONCLUSION： Paeoniflorin produces significant sedative and hypnotic effects, and there is an obvi- ous dose-effect relationship.     

Pharmacology and Toxicology of Extract from Arcangelisia gusanlung

Objective To study the pharmacology and toxicology of the extracts from Arcangelisia gusanlung(EAG). Methods The anti-inflammatory activities were investigated using various inflammatory models including ear edema induced by xylene in mice, paw edema induced by carrageenan, and cotton pellet granuloma in rats. The analgesic effect was observed in hot-plate test and writhing test in mice and the antipyretic effect was observed in rat fever model induced by yeast. The antitussive action was tested in mice by sequential method and expectorant action was evaluated by tracheal excretion of phenol red. The antidiarrhea function was observed on normal intestinal propulsion of mouse model of diarrhea induced by decoction of Sennae Folium. The toxicity was measured by toxicological experiment.Results Each dose of EAG could significantly inhibit the paw edema, cotton pellet granuloma, and intestinal propulsion. EAG significantly reduced writhing times and amount of wet manure. Obvious antipyretic action to fevered rat was observed. EAG obviously increased the tracheal excretion of phenol red and prolonged the latency of cough. No toxic reaction was shown in the observed period, and the maximum tolerance dose of mice was equivalent to 1360 times of common-used dose in human. Conclusion The clinical dosage of EAG is safe, and its anti-inflammatory, analgesia, antipyresis, antitussive, expectorant, and antidiarrhea effects are significant.     

Using the Box–Behnken Response Surface Method to Optimize the Preparation and Characterization of Lavender and Fennel Volatile oil β-cyclodextrin Form Inclusion Complex

Objective:The objective of this study is to study the best inclusion technology of Lavender-and fennel-mixed volatile oil by beta?cyclodextrin(β-CD) and characterize the final product thereafter.Methods:Using the saturated water solution method,the volatile β-CD inclusion complex was produced.The effect of volatile oil weight ratio,inclusion temperature and inclusion time on the inclusive quality was studied by measuring the yield of inclusion and inclusion rate of volatile oil as evaluation indexes.The preparation method of inclusion complex was then optimized by the Box-Behnken response surface method.The inclusion complex was characterized by ultraviolet spectrophotometry,thin-layer chromatography,thermogravimetry and differential thermal analysis,and the microscopic imaging method.Results:The optimized conditions were the weight ratio of β-CD to volatile oil was 8.13:1(g/ml).The inclusion temperature was 44°C.The inclusion time was 1 h.Conclusion:We were able to produce an inclusion complex with high inclusion rate of volatile oil and high yield of inclusion using the preparation method mentioned above.Furthermore,the method can also improve the stability of volatile oil in abnormal savda munziq.This study can provide a good reference for the development of new preparations.     

Analgesic and Anti-inflammatory Effects of Ginger Oil

Objective Ginger(Zingiber officinale) is widely used as a spice in cooking and as a medicinal herb in traditional herbal medicine.The present study was to investigate the analgesic and anti-inflammatory activities of ginger oil in experimental animal models.Methods The analgesic effect of the oils was evaluated by the "acetic acid" and "hot-plate" test models of pain in mice.The anti-inflammatory effect of the oil was investigated in rats,using rat paw edema induced by carrageenan,adjuvant arthritis,and vascular permeability induced by bradykinin,arachidonic acid,and histamine.Indomethacin(1 mg/kg),Aspirin(0.5 g/kg) and Dexamethasone(2.5 mg/kg) were used respectively as reference drugs for comparison.Results The ginger oil(0.25-1.0 g/kg) produced significant analgesic effect against chemically-and thermally-induced nociceptive pain stimuli in mice(P < 0.05,0.01).And the ginger oil(0.25-1.0 g/kg) also significantly inhibited carrageenan-induced paw edema,adjuvant arthritis,and inflammatory mediators-induced vascular permeability in rats(P < 0.05,0.001).Conclusion These findings confirm that the ginger oil can be used to treat pain and chronic inflammation such as rheumatic arthritis.     

ANALGESIA EFFECT OF ACUPUNCTURE AND MOXIBUSTION IN C57 MICE

Objective: To evaluate the antinociceptive effects of acupuncture and moxibustion on acute pain(formalin) and chronic pain(Freund's complete adjuvant, FCA) in mice.Methods: In the formalin test, C57 mice(n=40) were randomlydivided into control group, model group, acupuncture group and moxibustion group.Saline solution was injected into the hindpaw in the control group, while 1% formalin was injected into the hind-paw of the same side of rats in the model group, the acupuncture group and the moxibustion group.After injection, we recorded the time licking feet in mice at the first phase(0-5 min) and the second phase(15-60 min).In the FCA model, mice(n=40) were randomly divided into control group, model group, acupuncture group and moxibustion group.Saline solution was injected into the hind-paw in the control group, while 10 μL FCA was injected into the hind-paw of the same side in the model group, the acupuncture group and the moxibustion group.Analgesia effect was measured by von Frey test.Results: Noticeable antinociceptive effects after acupuncture and moxibustion treatment were observed in the second phase(P0.05) of th e formalin test.However, no significant difference between acupuncture and moxibution treatment was found(P0.05).In the FCA model, remarkable antinociceptive effects after acupuncture and moxibustion treatment were observed in 15 to 90 min(P0.05).Also, the pain threshold in the moxibustion group was higher than that in the acupuncture group at 15 min(P0.05) and 30 min(P0.01).Conclusion: Acupuncture and moxibustion have demonstrated efficacy on the inflammatory pain in the treatment of acute pain.Therapeutic effect of moxibustion is higher than that of acupuncture in the treatment of chronic pain.     

丹红注射液联合依达拉奉注射液治疗急性脑梗死58例临床观察

Objective To observe clinical effect of treating acute cerebral infarction with Danhong injection combined with Edaravone injection. Methods 58 cases with acute cerebral infarction were randomly recruited into a control group and a treatment group. The control group was treated with edaravone injection, 30 mg, once daily, and for continuous 14 days. The treatment group was treated with dan hong injection, 20 ml, once daily, and for continuous 14 days based on the control group. The clinical effect and adverse reaction were observed in both groups after the treatment. Results The total effective rate was 86.2% and 62.1% in the treatment group and the control group respectively. The clinical effect in the treatment group was significantly higher than that of the control group (χ2=3.561, P＜0.05). Conclusion Danhong injection combined with edaravone injection was more effective in treating acute cerebral infarctionthan edaravone injection exclusively.     

Protection of Water Extract from Paeoniae Radix Rubra against Myocardial Ischemia in Mice Induced by Isoproterenol

Objective To study the protective effect of the water extract from Paeoniae Radix Rubra(WEPRR)against myocardial ischemia in mice induced by isoproterenol(ISO).Methods The mice were randomly divided into six groups:positive control,normal control,model,low-,mid-,and high-dose[0.3,0.6,and 1.2 g/(kg·d)]WEPRR groups.The mice in WEPRR groups were ig administered with WEPRR,the mice in the positive control group were ig administered with 0.98%Di’ao Xinxue Kang[(0.195 g/(kg·d)],and the mice in normal and model groups were ig administered with the same volume of physiological saline once daily for consecutive 11 d.On the day 7 from the beginning of the ig administration,the mice in the model and WEPRR groups were ip perfused with 0.02 g/(kg·d)ISO.After 15 min of the last medication,the mice were anesthetized with isoflurane gas,the blood was collected through venous sinus of eye orbit,then the mice were killed.The heart tissues were rapidly removed from the mice,washed in physiological saline,soaked in filter paper,and stored in 80℃until use.The activities of lactate dehydrogenase(LDH),creatine kinase-MB(CK-MB),creatine kinase(CK)in serum,superoxide dismutase(SOD),and the contents of malondialdehyde(MDA)in the heart of mice were determined,respectively.Results Compared with the model group,the activities of LDH,CK-MB,and CK in serum,and the SOD of mice in the positive control and WEPRR groups were increased and the content of MDA in heart was decreased.Conclusion The WEPRR has the cardioprotective activities on ISO-induced myocardial ischemia.     

Berberine reverses LPS-induced repression of CYP7A1 through an anti-inflammatory effect

Objective: To evaluate the anti-inflammatory effect of berberine(BBR) on the lipopolysaccharide(LPS)-induced acute phase response and its modulation of the altered bile acid metabolism induced by LPS treatment.Methods: An acute phase response was induced by intraperitoneal injection of LPS(5 mg/kg, ip) in C57 BL/6 J mice, and the BBR treatment group was orally administered with BBR(200 mg/ky, ig). The levels of TNFα, IL-1β and IL-6 in the serum were measured using an ELISA kit, and their expression levels in the liver were measured using q RT-PCR. The bile acid pool was measured using a commercial bile acid kit, and the expression levels of enzymes involved in bile acid metabolism were measured by q RTPCR. The expression levels of CYP7 A1, p65 NF-κ B and the MAPK signaling pathway was measured using Western blotting.Results: LPS treatment suppressed the expression of CYP7 A1 and CYP8 B1, and the total bile acid pool was also reduced. Pretreatment with BBR inhibited the pro-inflammatory biomarkers TNFα and IL-1β in the serum, as well as the expression of TNFα, IL-1β and i NOS mRNA in the liver. BBR treatment did not affect the reduction in the bile acid pool size induced by LPS, but significantly increased the concentration of bile acids in the liver, which was consistent with the upregulated expression of CYP7 A1 and CYP8 B1. The MAPK signaling pathway was activated by BBR treatment, while the p65 NF-κ B signaling pathway was inhibited.Conclusion: BBR can offer an anti-inflammatory effect and reverse the inhibition of CYP7 A1 and CYP8 B1 expression caused by LPS treatment, as well as induce the production of bile acids in liver, probably via MAPK signaling; However, treatment with BBR had no effect on the size of total bile acid pool.     

Evaluation of Antinociceptive Activity of Methanol Extract from Cleome rutidosperma in Mice

Objective Cleome rutidosperma (Capparidaceae), commonly known as “Fringed Spider Flower”, is a medicinal plant found in Southeast Asia. C. rutidosperma is used in folk medicine for diuretic, laxative, analgesic, anti-inflammatory, antipyretic, antimicrobial, anti-oxidant, hypoglycemic, and anthelmintic activities. We have evaluated the anti-nociceptive properties of methanol extract from C. rutidosperma (MECR) in vivo. Methods Thermal method (hot plate test and tail flick test) was induced to judge the anti-inflammatory effect and couple of chemical method also used (formalin induced licking test; writhing test carried by acetic acid) to evaluate analgesic effect. Both of these tests were made over animal models, like mice and rats. Two different doses (100 and 200 mg/kg) were used for each case of test, while morphine sulphate (5mg/kg, ip) was used as reference drug. Results MECR demonstrated the significantly anti-nociceptive activity in the analgesic and anti-inflammatory tests by reducing nociception in mice models (P < 0.001). In the hot-plate and tail-flick tests, MECR significantly elongated the time to response to the thermal stimuli (100 and 200 mg/kg with P < 0.05, 0.001). The remarkable increase in the latency was observed at 90 and 120 min. In acetic acid-induced writhing test and formalin induced licking test for anti-inflammatory activity, MECR at 100 and 200 mg/kg doses exhibited significant (P < 0.001) reduction of writhing and licking response. Conclusion The anti-inflammatory and analgesic effects of C. rutidosperma propose that this effect may be a result of both peripheral and central mechanisms. Further study is required to ensure the proper mechanism of action as well as the active ingredient.     

Correlation between slow transit constipation and spleen Qi deficiency,and gut microbiota: a pilot study

OBJECTIVE: To investigate the effect of slow transit constipation(STC) and spleen Qi deficiency on gut microbiota, and the mechanism underlying the action that the positive drug Maren Runchang(MR) alleviates STC. METHODS: STC was induced, using the cathartic method of Senna and the hunger-fullness disorder method, in ICR mice; one group of model mice was treated with MR(6.24 g/kg). The changes in the general condition, fecal parameters, D-xylose content in the serum, intestinal propulsion rate, ...     

黑水缬草挥发油对中枢神经系统药理作用的研究

目的：观察黑水缬草挥发油对中枢神经系统的作用。方法：考察黑水缬草挥发油对小鼠自主活动的影响、对阈下和催眠剂量戊巴比妥钠的睡眠协同作用、对大鼠睡眠时相的影响、对醋酸所致小鼠扭体反应的影响以及对硫代氨基脲诱发小鼠惊厥的影响。结果：黑水缬草挥发油对小鼠的自主活动具有显著的抑制作用，与戊巴比妥钠有较好的催眠协同作用，可提高小鼠的入睡率，延长小鼠睡眠时间，明显延长大鼠睡眠周期中的慢波睡眠Ⅱ期（SWS2）和快动眼睡眠期（REMS）两个时相，同时能明显减少小鼠扭体反应次数，对抗硫代氨基脲诱发的小鼠惊厥。结论：黑水缬草挥发油具有显著的镇静、镇痛和抗惊厥等中枢神经系统抑制作用，预示了良好的新药开发前景。     

清宫汤镇静作用的实验研究

目的研究清宫汤镇静、催眠的药理作用。方法50只小鼠随机分成5组，即生理盐水组、安定组及清宫汤低、中、高剂量组，安定组给予0．1mL／10g灌胃给药，其他4组按照0．2mL／10g灌胃给药，记录给药前后自主活动，测定给予戊巴比妥钠闽上催眠剂量小鼠的睡眠时间及统计阁下剂量的睡眠动物数，另采用尼可刹米致惊厥小鼠模型，观察给予清宫汤后小鼠惊厥发生率的变化。结果清宫汤3．0、6．0g／kg剂量能明显抑制小鼠的自主活动，延长戊巴比妥钠阈上剂量的小鼠睡眠时间，增加戊巴比妥钠阁下催眠剂量所致睡眠动物数，并能显著对抗尼可刹米致小鼠惊厥的作用。结论清宫汤有明显的镇静、催眠、抗惊厥作用。     

灵芝生料酿制液镇静催眠作用的研究

目的考察灵芝生料酿制液(GLB)镇静催眠药理作用。方法取NIH小鼠连续灌胃0.06,0.12,0.24g/kgGLB15d,测定GLB对实验小鼠自发活动情况,对戊巴比妥钠阈下剂量、阈剂量小鼠睡眠时间和对戊四氮致小鼠惊厥的作用。结果GLB灌胃可减少小鼠自发活动,显著延长阈上剂量戊巴比妥钠致小鼠睡眠时间,增加阈下剂量戊巴比妥钠睡眠动物数,GLB与戊巴比妥钠有协同作用。有对抗戊四氮致惊厥发作的作用,且剂量越大,作用越明显。结论GLB有明显的镇静、催眠、抗惊厥等中枢抑制作用。     

Anticonvulsant activities of nutmeg oil of Myristica fragrans

The purpose of this study was to investigate the anticonvulsant activity of the volatile oil of nutmeg, the dried seed kernel of Myristica fragrans Houtt, using well‐established animal seizure models and to evaluate its potential for acute toxicity and acute neurotoxicity. The volatile oil of nutmeg (nutmeg oil) was tested for its effects in maximal electroshock, subcutaneous pentylenetetrazole, strychnine and bicuculline seizure tests. All the experiments were performed at the time of peak effect of nutmeg oil. Nutmeg oil showed a rapid onset of action and short duration of anticonvulsant effect. It was found to possess significant anticonvulsant activity against electroshock‐induced hind limb tonic extension. It exhibited dose dependent anticonvulsant activity against pentylenetetrazole‐induced tonic seizures. It delayed the onset of hind limb tonic extensor jerks induced by strychnine. It was anticonvulsant at lower doses, whereas weak proconvulsant at a higher dose against pentylenetetrazole and bicuculline induced clonic seizures. Nutmeg oil was found to possess wide therapeutic margin, as it did not induce motor impairment when tested up to 600 µL/kg in the inverted screen acute neurotoxicity test. Furthermore, the LD₅₀ (2150 µL/kg) value was much higher than its anticonvulsant doses (50–300 µL/kg). The results indicate that nutmeg oil may be effective against grand mal and partial seizures, as it prevents seizure spread in a set of established animal models. Slight potentiation of clonic seizure activity limits its use for the treatment of myoclonic and absence seizures. Copyright © 2008 John Wiley & Sons, Ltd.     

Pharmacological activities investigation of crude extracts and fractions from Qualea grandiflora Mart

This study investigates pharmacological activities of crude hydroalcoholic extract and fractions of Qualea grandiflora Mart. leaves employing different experimental models using mice. The treatment with crude hydroalcoholic extract (EH) in a dose of 500 mg/kg, i.p. caused: signs of central nervous system depressant action in the Hippocratic screening test, confirmed by the potentiation of sodium pentobarbital sleeping time. Increasing in the latency time of hot plate assay that indicate an analgesic effect; significantly delaying of the onset of clonic PTZ convulsions, increasing in the time for death, suppressing of the tonic PTZ convulsion, and decreasing of severity and number of convulsions. The median lethal dose of EH was 1.321 mg/kg. The convulsions induced by PTZ, ethyl ether fraction (300 mg/kg, i.p.) was more active in increasing the latency time for first convulsion, moreover, the hexane fraction, at the same dose, was more active in increasing the time for death and/or avoiding the death. Both did not cause disturbance in motor coordination at the dose of 500 mg/kg, assessed by rotarod test. These results suggest that the crude extract of leaves of Qualea grandiflora Mart. has a central nervous system depressant action, an analgesic effect and behave as a potential anticonvulsant.     

天麻成分D的急性毒性实验及对小鼠睡眠影响的研究

目的:了解天麻成分D灌胃给药的急性毒性,为其进行一步研究提供依据;观察天麻成分D对戊巴比妥钠致小鼠催眠作用的影响。方法:选择SPF级昆明种小鼠为受试对象,采用急性经口毒性试验测定半数致死量(LD50)和最大耐受量(MTD);采用对阈上剂量和阈下剂量戊巴比妥钠所致催眠作用的影响试验来评价药物对中枢神经系统的作用。结果:天麻成分D小鼠ig给药的LD50为1.2069g/kg体重,LD5为0.93464g/kg,MTD为0.8717g/kg;天麻成分D小鼠ig给药,单独使用并不能引起小鼠睡眠,但可以使戊巴比妥钠ip引起的小鼠睡眠作用增强,睡眠潜伏期缩短,睡眠持续时间延长。结论:天麻成分D小鼠ig给药有一定毒性,LD50为1.2069g/kg体重;天麻成分D小鼠ig给药对中枢神经系统可能有一定的抑制作用,不能直接引起小鼠睡眠,但可增强戊巴比妥钠对小鼠的催眠作用。     

五味子宁神口服液的镇静及催眠作用研究

目的对五味子宁神口服液的镇静、催眠、抗惊厥药理作用进行初探。方法采用镇静、催眠、抗惊厥实验观察五味子宁神口服液对小鼠自主活动的影响,协同戊巴比妥钠对小鼠的催眠作用及对小鼠尼可刹米惊厥的抑制作用。结果五味子宁神口服液能明显减少小鼠自主活动次数,增加阈下剂量戊巴比妥钠致小鼠睡眠只数,延长阈上剂量戊巴比妥钠致小鼠睡眠时间,延长小鼠发生惊厥的潜伏期及减少小鼠死亡只数。结论五味子宁神口服液有明显的镇静、催眠、抗惊厥作用。     

天麻灵芝颗粒药效学实验研究

目的对天麻灵芝颗粒进行药效学研究。方法通过小鼠断头张口喘气实验、耐常压缺氧实验、豚鼠氯仿所致眩晕实验、旋转刺激缺血性眩晕模型大鼠水迷宫实验,考察天麻灵芝颗粒抗眩晕作用;通过小鼠自主活动实验、小鼠协同戊巴比妥钠催眠实验,考察天麻灵芝颗粒的镇静催眠作用;通过小鼠扭体法实验、小鼠热板法实验,考察天麻灵芝颗粒镇痛作用;通过游泳和调节免疫实验,考察天麻灵芝颗粒抗疲劳及调节免疫的作用。结果天麻灵芝颗粒能促进大脑供血供氧;能明显减少小鼠的自主活动次数,增加阈下剂量戊巴比妥钠睡眠小鼠只数,缩短阈剂量戊巴比妥钠小鼠入睡潜伏期并延长睡眠时间;能显著地增加醋酸致小鼠扭体反应潜伏期并减少扭体反应次数,增加热板法小鼠痛阈值;能明显延长小鼠游泳时间,增加幼龄小鼠脾脏、胸腺指数,增加小鼠的碳粒廓清指数和吞噬指数。结论天麻灵芝颗粒具有抗眩晕、镇静催眠、镇痛、抗疲劳及提高免疫功能的作用。     

综合评价安眠方剂对小白鼠戊巴比妥钠催眠、阈下催眠量影响随机平行对照研究

[目的]观察安眠方剂对小白鼠戊巴比妥钠催眠、阈下催眠量影响。[方法]使用随机平行对照方法,将410只健康昆明种小鼠编号按抽签随机分组,催眠作用组（安眠1号组、安眠2号组、安眠3号组、安眠4号组、百乐眠胶囊组、地西泮片组、空白对照组,10R／组）,催眠量组（安眠l号组、安眠2号组、安眠3号组、安眠4号组、百乐眠胶囊组、地西泮片组、空白对照组,20只／组）,急性毒性组（安眠1号组、安眠2号组、安眠3号组、安眠4号组,50只／组）。戊巴比妥钠催眠作用,各组灌胃给药40mL／kg,空白对照组给等容积蒸馏水,1次／d,连续给药5d。各组末次给药后50min,腹腔注射50mg／kg戊巴比妥钠,从开始注射戊巴比妥钠到翻正反射消失时间为入睡潜伏期,从翻正反射消失到翻正反射恢复时间为睡眠时间。戊巴比妥钠闽下催眠量,各组灌胃给药40mL／kg,空白对照组给等容积蒸馏水,1次／d,连续给药5d。各组末次给药50min后,腹腔注射35mg／kg戊巴比妥钠（生理盐水配制）,以给药后15min内小鼠翻正反射消失lminvX上的鼠数作为入睡指标。小鼠急性毒性,各组24h灌胃给药1次,给药眠1号药组61．60g／kg,安眠2号药组55．60g／kg,安眠3号药组50．00g／kg,安眠4号药组62．80g／kg;各给药组按剂量距l：0．90等比级数递减灌胃给药,容积为40mL／Kg。一次灌胃给药后立即观察动物24h反应,并连续14d观察动物毒性反应和死亡情况。[结果]入睡潜伏期安眠1号组与空白对照组无明显差异（P〉0．05）,其余各干预组均明显高于空白对照组（P〈0．01,P〈0．05）;睡眠时间各干预组均明显高于空白对照组（P〈0．01）;入睡鼠数安眠3号组与空白对照组无明显差异（P〉0．05）,其余各干预组均明显高于空白对照组（P〈0．01）。安眠1号组LD50为45-35g／kg～50．66g／kg,95％可信限45．35g／kg～50．66g／kg。安眠2号组LD50为44．14g／kg,95％可信限41.38g/kg～47．09g／kg。安眠3号组LD50为36．20g／kg,95％可信限34．29g／kg～38．21g/kg,安眠4号组LD50为16．41g/kg,95％可信限为12．91g／kg～20．85g／kg。安眠1号药组为18000。[结论]本试验将药效、毒性综合评估的方法运用到临床前药物研究,将药效与毒性分别进行三个级别赋分,根据药效／毒性比值,确定安眠1号药处方。     

复方酸枣安神胶囊催眠作用实验研究

目的研究复方酸枣安神胶囊对小鼠的镇静催眠作用。方法采用小鼠自主活动记数法研究复方酸枣安神胶囊的镇静作用,通过直接诱导小鼠睡眠,观察阈上、阈下剂量戊巴比妥钠对小鼠睡眠的协同作用。结果复方酸枣安神胶囊能明显降低小鼠的自主活动次数,延长阈上剂量戊巴比妥钠小鼠的睡眠时间,减少睡眠潜伏期;未见对小鼠有明显直接诱导睡眠作用,但与阈下剂量戊巴比妥钠对小鼠睡眠具有明显的协同作用,使入睡小鼠个数显著增加。结论复方酸枣安神胶囊具有一定的镇静催眠作用。