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1.
锦灯笼化学成分   总被引:3,自引:0,他引:3  
目的:研究锦灯笼的化学成分。方法:采用硅胶、ODS柱色谱、Sephadex LH-20和半制备液相色谱等技术分离纯化,结合ESI-MS,UV,NMR等现代波谱学技术进行结构鉴定。结果:分离并鉴定了8个化合物,分别为(+)-丁香脂素-O-β-D-双吡喃葡萄糖苷(1),(+)-松脂酚-O-β-D-双吡喃双葡萄糖苷(2),(+)-杜仲树脂酚-O-β-D-双吡喃葡萄糖苷(3),芹菜素-7-O-β-D-葡萄糖苷(4),金圣草黄素-7-O-β-D-葡萄糖苷(5),香叶木素-7-O-β-D-葡萄糖苷(6),莨菪亭-7-O-β-D-葡萄糖苷(7)和对羟基苯乙醇-(阿魏酸-4-O-β-D-葡萄糖酯)苷(8)。结论:化合物1~8均为首次从该植物中分离得到。  相似文献   

2.
泥胡菜黄酮类化学成分研究   总被引:1,自引:1,他引:0  
目的:研究泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位化学成分。方法:采用硅胶柱色谱,ODS柱色谱,Sephadex LH-20柱色谱和制备液相色谱等手段进行化学成分分离,运用理化性质和波谱数据(MS,1 H-NMR,13 C-NMR,HMBC等)鉴定化合物结构。结果:从泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位分离得到8个黄酮类化合物,分别鉴定为金合欢素(1),玄参黄酮(2),芹菜素(3),山萘酚(4),山萘酚-3-O-β-D-吡喃葡萄糖苷(5),山萘酚-7-O-β-D-吡喃葡萄糖苷(6),山萘酚-3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(7),金合欢素-7-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(8)。结论:化合物5为首次从该属植物中分离得到。  相似文献   

3.
籽瓜汁化学成分的分离与鉴定   总被引:1,自引:0,他引:1  
目的:对籽瓜汁的化学成分进行研究。方法:通过反复硅胶柱色谱和羟丙基葡聚糖凝胶柱色谱方法进行分离纯化,运用核磁共振氢谱、碳谱技术和质谱技术进行结构鉴定。结果:共分离鉴定8个化合物。分别鉴定为3,5-二甲氧基-苄醇-4-O-β-D-吡喃葡萄糖苷(1),2,6-二甲氧基-4-羟基-苯酚-1-O-β-D-吡喃葡萄糖苷(2),1-O-对香豆酰基甘油酯(3),松柏苷(4),cucumegastigmanes II(5),linarionoside C(6),δ-葡萄素(7),山柰酚-3-O-葡萄糖基1-O-β-D-葡萄糖-6-O-α-L-鼠李糖苷(8)。结论:化合物1~8均为首次从籽瓜中分离得到。  相似文献   

4.
目的:研究裸花紫珠95%乙醇提取物的化学成分。方法:采用硅胶,MCI,C_(18)等柱色谱方法对裸花紫珠中化学成分进行分离纯化,并经波谱数据分析和文献对照鉴定化合物结构。结果:从裸花紫珠叶95%乙醇提取物中分离得到8个化合物,分别鉴定为木犀草苷(1),木犀草素-3'-O-β-D-吡喃葡萄糖苷(2),木犀草素-4'-O-β-D-吡喃葡萄糖苷(3),芹菜素-7-O-β-D-葡萄糖醛酸苷丁酯(4),木犀草素-3'-O-(6″-E-咖啡酰)-β-D-吡喃葡萄糖苷(5),木犀草素-4'-O-(6'-E-咖啡酰)-β-D-吡喃葡萄糖苷(6),木犀草素-7-O-(6″-E-咖啡酰)-β-D-吡喃葡萄糖苷(7),木犀草素-7-O-(6″-E-阿魏酰)-β-D-吡喃葡萄糖苷(8)。结论:化合物4为首次从该植物分离得到。  相似文献   

5.
目的:研究忍冬藤Lonicera japonica Thunb 的化学成分.方法:采用硅胶、Sephadex LH-20和MCI HP-20等柱色谱方法进行分离纯化,并根据化合物的理化性质和波谱数据鉴定其结构.结果:从忍冬藤醇提物中分离得到13个化合物,分别鉴定为原儿茶酸(1),咖啡酸(2),灰毡毛忍冬素G(3),七叶内酯(4),木犀草素(5),槲皮素(6),芹菜素(7),木犀草素-7-O-β-D-吡喃葡萄糖苷(8),异鼠李素-7-O-β-D-吡喃葡萄糖苷(9),香叶木素-7-O-β-D-吡喃葡萄糖苷(10),(11),忍冬苷(12),Hydnocarpin D(13).结论:化合物4,7,9~11为首次从本植物中分离得到,化合物13为首次报道从本属植物中分离得到的黄酮木脂素.  相似文献   

6.
沙生蜡菊花中的黄酮类成分   总被引:3,自引:1,他引:2  
目的:沙生蜡菊花的化学成分研究.方法:采用多种色谱方法(硅胶柱色谱、ODS柱色谱和HPLC柱色谱)分离纯化,依据理化性质、波谱数据分析进行结构鉴定.结果:从沙生蜡菊花的甲醇提取物中分离得到7个黄酮类化合物,分别鉴定为槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,1),芹菜素-7-O-β-D-龙胆二糖苷(apigenin 7-O-β-D-gen-tiobioside,2),槲皮素3-O-芸香糖苷(quercetin 3-O-rutinoside,3),芹菜素-7,4'-二-O-葡萄糖苷(apigenin-7,4'-di-O-β-D-glucopyranasideside,4),山柰酚-3,4'-二-O-葡萄糖苷(kaempferol-3,4'-di-O-β-D-glucopyranoside,5),槲皮素-3,3'-O-二葡萄糖(quercetin-3,3'-di-O-β-D-glucopyranaside,6),芹菜素-7-O-β-D-葡萄糖醛酸甲酯(apigenin-7-O-β-D-glucopyranosiduronic acid methyl ester,7).结论:化合物2,4~7为首次从该属植物中分离得到.  相似文献   

7.
野菊花心血管活性部位化学成分的研究   总被引:1,自引:0,他引:1  
目的:研究菊科植物野菊花心血管活性部位中的主要化学成分.方法:采用多种色谱方法进行分离纯化,通过波谱方法结合文献对照鉴定化合物结构.结果:从野菊花心血管活性部位中分离并鉴定了12个化合物,包括7个黄酮类化合物(1~7),5个苯丙素类化合物(8~12),分别鉴定为(2S)-圣草酚-7-O-β-D-吡喃葡萄糖醛酸苷(1),(2S)-圣草酚-7-O-β-D-吡喃葡萄糖苷(2),(2S)-橙皮素-7-O-β-D-吡喃葡萄糖醛酸苷(3),木犀草素-7-O-β-D-吡喃葡萄糖苷(4),木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷(5),香叶木素-7-O-β-D-吡喃葡萄糖醛酸苷(6),槲皮素-7-O-β-D-呲喃葡萄糖苷(7),3,5-二咖啡酰奎宁酸甲酯(8),3,5-二咖啡酰奎宁酸(9),3,5-二顺式咖啡酰奎宁酸(10),1,5-二咖啡酰奎宁酸(11),1,3-二咖啡酰奎宁酸(12).结论:野菊花心血管活性部位中主要成分为黄酮类化合物,共5个化合物分别为2~3,7~8,10;共10个化合物分别为2~3,5~12系首次从该种植物分离得到.  相似文献   

8.
目的:研究民间传统彝族药鞭打绣球的化学成分。方法:利用硅胶柱色谱、凝胶色谱、高效液相色谱法等分离方法分离纯化鞭打绣球的化学成分,并根据化合物的理化性质及波谱数据鉴定其结构。结果:分离得到8个黄酮及黄酮苷类化合物,分别鉴定为:木樨草素(1),芹菜素(2),槲皮素(3),桑色素(4),5,3',4'-三羟基-7-甲氧基黄酮(5),胡桃宁(6),山奈酚-7-O-β-D-吡喃葡萄糖苷(7),木樨草素-7-O-β-D-吡喃葡萄糖(1→6)-β-D-吡喃葡萄糖苷)(8)。结论:化合物1-8均为首次从该属植物中分离得到。  相似文献   

9.
甘松根茎化学成分研究   总被引:5,自引:0,他引:5  
Liu CL  Duan YH  Dai Y  Yao XS 《中药材》2011,34(8):1216-1219
目的:研究甘松根茎的化学成分。方法:运用大孔树脂Diaion HP-20、硅胶、反相ODS、Sephadex LH-20等柱色谱方法,结合制备型HPLC进行分离纯化,根据理化性质和MS、NMR等波谱学数据进行结构鉴定。结果:从甘松根茎中分离鉴定了6个化合物,分别为:8-羟基松脂醇-4-O-β-D-吡喃葡萄糖苷(1)、8-羟基松脂醇-4'-O-β-D-吡喃葡萄糖苷(2)、8-羟基松脂醇-4-O-(β-D-吡喃葡萄糖基)-4'-O-β-D-吡喃葡萄糖苷(3)、环橄榄脂素-6-O-β-D-吡喃葡萄糖苷(4)、阿魏酸(5)、绿原酸甲酯(6)。结论:化合物1~6均为首次从该属植物中分离得到。  相似文献   

10.
目的:研究黑水缬草(Valeriana amurensis Smir.ex Kom.)治疗老年痴呆有效部位化学成分。方法:采用大孔树脂吸附柱色谱、正相硅胶柱色谱、ODS反相柱色谱、HPLC等分离方法及各种波谱学鉴定方法。结果:从大孔树脂吸附柱色谱的50%乙醇洗脱组分中分离并鉴定了13个化合物,分别为:(+)松脂素-4,4'-O-β-D-双葡萄吡喃糖苷(1),(+)8-羟基-松脂素-4'-O-β-D-葡萄吡喃糖苷(2),(+)8-羟基-松脂素-4-O-β-D-葡萄吡喃糖苷(3),(+)松脂素-8-O-β-D-葡萄吡喃糖苷(4),(+)松脂素-4-O-β-D-葡萄吡喃糖苷(5),(+)8-羟基-松脂素(6),(+)8,9'-二羟基-松脂素-4'-O-β-D-葡萄吡喃糖苷(7),(+)8-羟基-松脂素-4,4'-O-β-D-双葡萄吡喃糖苷(8),橄榄树脂素-4'-O-β-D-葡萄糖苷(9),落叶松脂醇-4,4'-O-β-D-双葡萄吡喃糖苷(10),橄榄树脂素-4-O-β-D-葡萄吡喃糖苷(11),8-羟基-落叶松脂醇-4'-O-β-D-葡萄吡喃糖苷(12),落叶松脂醇-4-O-β-D-葡萄吡喃糖苷(13)。结论:13个化合物全部为该植物中首次发现,其中包括8个双环氧木脂素(1~8)和5个单环氧木脂素(9~13)。  相似文献   

11.

Ethnopharmacological relevance

Mucuna pruriens is a tropical legume anecdotally reputed to have anthelmintic properties. This study was conducted to examine the validity of such claims.

Aim of the study

The aim of this study was to determine if ingestion of Mucuna seeds reduces helminth parasite infestation in lambs.

Materials and methods

Thirty-six Dorper × Katahdin ram lambs were assigned to three treatments, a cottonseed meal based control diet, a diet in which Mucuna replaced cottonseed meal and the control diet with levamisole (7.5 mg/kg body weight) administration. All diets were isonitrogenous and isocaloric. The 12 lambs in each treatment were assigned randomly to 4 pens, each containing 3 lambs. Lambs were trickle infected three times per week by gavage with infectious Haemonchus contortus larvae (2000 larvae/lamb) for 3 weeks.

Results

Levamisole treatment decreased fecal egg counts by 87% and abomasal worm counts by 83%. Mucuna intake did not statistically affect fecal egg counts or abomasal worm counts, though numerical (P > 0.10) reductions of 7.4% and 18.1%, respectively were evident. Anemia indicators, feed intake, and lamb growth were unaffected by treatment.

Conclusions

Levamisole reduced the Haemonchus parasite burden in lambs significantly but feeding Mucuna reduced the burden by levels unlikely to eliminate the clinical effects of parasitism.  相似文献   

12.
汪长中  王龙海 《中国中药杂志》2010,35(13):1769-1772
近年来真菌感染率逐年上升,传统抗真菌药物易产生耐药性,而中药在防治真菌感染方面具有一定的优势。本文就近5年来中药对白念珠菌、皮肤癣菌、曲霉菌、马拉色菌、串珠镰孢菌、申克孢子丝菌、新生隐球菌及真菌生物膜的干预研究进展进行综述。  相似文献   

13.
厚朴与凹叶厚朴群体遗传学研究   总被引:3,自引:0,他引:3  
目的:对厚朴与凹叶厚朴的群体遗传学进行研究,为中药厚朴的质量控制提供分子生药学方面的依据。方法:对厚朴与凹叶厚朴15个居群应用2个叶绿体基因间序列psbA-trnH和trnL-trnF进行PCR扩增并测序,计算厚朴与凹叶厚朴单倍型频率,用程序HaploNst分析遗传多样性和遗传结构,应用TCS version 1.13软件构建单倍型网状进化树。结果:厚朴与凹叶厚朴均无特有单倍型存在,但单倍型频率存在显著差异,已开始出现遗传分化的趋势,NST略大于GST。结论:厚朴与凹叶厚朴在遗传上已出现遗传分化的趋势,但尚未完全分化成彼此独立的单系。  相似文献   

14.

Aim of the study

To investigate the activities of the 217 plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts.

Materials and methods

Plant extracts were prepared by the method of maceration using solvents of different polarities. The growth inhibition of chloroquine-resistant Plasmodium falciparum strain (FcB1) was determined by measuring the radioactivity of the tritiated hypoxanthine incorporated. Activity against Leishmania (Leishmania) chagasi and Trypanosoma cruzi was measured by the MTT colorimetric assay. The antifungal tests were carried out by using the CLSI method. The active extracts were tested also by cytotoxicity assay using NIH-3T3 cells of mammalian fibroblasts.

Results

Two hundred and seventeen extracts of plants were tested against Plasmodium falciparum. The eleven active extracts, belonging to eight plant species were evaluated against L. (L.) chagasi, Trypanosoma cruzi, yeasts and in NIH-3T3 cells. The results found in these biological models are consistent with the ethnopharmacological data of these plants. The ethyl acetate extract of Diospyros hispida root showed IC50 values of 1 μg/mL against Plasmodium falciparum. This extract demonstrated no toxicity against mammalian cells, resulting in a significant selectivity index (SI) of 435.8. The dichloromethane extract of Calophyllum brasiliense root wood was active against Cryptococcus gattii LMGO 01 with MIC of 1.95 μg/mL; and Candida albicans ATCC 10231 and Candida krusei LMGO 174, both with MIC of 7.81 μg/mL. The same extract was also active against Plasmodium falciparum and L. (L.) chagasi with IC50 of 6.7 and 27.6 μg/mL respectively. The ethyl acetate extract of Spiranthera odoratissima leaves was active against Cryptococcus gattii LMGO 01 with MIC of 31.25 μg/mL, and against Plasmodium falciparum with IC50 of 9.2 μg/mL and Trypanosoma cruzi with IC50 of 56.3 μg/mL.

Conclusion

The active extracts for protozoans and human pathogenic yeasts are considered promising to continue the search for the identification and development of leading compounds.  相似文献   

15.

Ethnopharmacological relevance

Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi L. (Oxalidaceae), Biophytum sensitivum (L.) DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam. (Rhizophoraceae) are used as remedies to control diabetes. In the present study, these plants were screened for their potential α-glucosidase inhibitory activity.

Materials and methods

The 80% aqueous ethanolic extracts were screened for their α-glucosidase enzyme inhibitory activity using yeast alpha glucosidase enzyme.

Results

Except for A. bilimbi with IC50 at 519.86±3.07, all manifested a significant enzyme inhibitory activity. R. mucronata manifested the highest activity with IC50 at 0.08±1.82 μg mL−1, followed by C. tagal with IC50 at 0.85±1.46 μg mL−1 and B. sensitivum with IC50 at 2.24±1.58 μg mL−1.

Conclusion

This is the first report on the α-glucosidase inhibitory effect of the six Philippine plants; thus, partly defining the mechanism on why these medicinal plants possess antidiabetic properties.  相似文献   

16.

Ethnopharmacological relevance

In particular five polypore species, i.e. Laetiporus sulphureus, Fomes fomentarius, Fomitopsis pinicola, Piptoporus betulinus, and Laricifomes officinalis, have been widely used in central European folk medicines for the treatment of various diseases, e.g. dysmenorrhoea, haemorrhoids, bladder disorders, pyretic diseases, treatment of coughs, cancer, and rheumatism. Prehistoric artefacts going back to over 5000 years underline the long tradition of using polypores for various applications ranging from food or tinder material to medicinal–spiritual uses as witnessed by two polypore species found among items of Ötzi, the Iceman. The present paper reviews the traditional uses, phytochemistry, and biological activity of the five mentioned polypores.

Materials and methods

All available information on the selected polypore taxa used in traditional folk medicine was collected through evaluation of literature in libraries and searches in online databases using SciFinder and Web of Knowledge.

Results

Mycochemical studies report the presence of many primary (e.g. polysaccharides) and secondary metabolites (e.g. triterpenes). Crude extracts and isolated compounds show a wide spectrum of biological properties, such as anti-inflammatory, cytotoxic, and antimicrobial activities.

Conclusions

The investigated polypores possess a longstanding ethnomycological tradition in Europe. Here, we compile biological results which highlight their therapeutic value. Moreover, this work provides a solid base for further investigations on a molecular level, both compound- and target-wise.  相似文献   

17.

Aim of the study

In a search for new plant-derived biologically active compounds against malaria parasites, we have carried out an ethnopharmacological study to evaluate the susceptibility of cultured Plasmodium falciparum to extracts and fractions from seven Cameroonian medicinal plants used in malaria treatment. We have also explored the inhibition of the Plasmodium falciparum cysteine protease Falcipain-2.

Materials and methods

Plant materials were extracted by maceration in organic solvents, and subsequently partitioned or fractionated to afford test fractions. The susceptibility of erythrocytes and the W2 strain of Plasmodium falciparum to plant extracts was evaluated in culture. In addition, the ability of annonaceous extracts to inhibit recombinant cysteine protease Falcipain-2 was also assessed.

Results and discussion

The extracts showed no toxicity against erythrocytes. The majority of plant extracts were highly active against Plasmodium falciparumin vitro, with IC50 values lower than 5 μg/ml. Annonaceous extracts (acetogenin-rich fractions and interface precipitates) exhibited the highest potency. Some of these extracts exhibited modest inhibition of Falcipain-2.

Conclusion

These results support continued investigation of components of traditional medicines as potential new antimalarial agents.  相似文献   

18.
目的:克隆金银花类药用植物4-二磷酸胞苷-2-C-甲基赤藓糖激酶(4-diphosphocytidyl-2-C-methyl-D-erythritol kinase,IspE)和4-羟基-3-甲基-2-邻苯基二磷酸还原酶(4-hydroxy-3-methylbut-2-enyl diphosphate reductase,IspH)基因,并对其基因序列、蛋白特性和转录活性进行分析、比较.方法:从忍冬Lonicera japonica转录组测序结果中分析获得了IspE,IspH基因.分别以忍冬、红白忍冬L.japonica var.chinensis、红腺忍冬L.hypoglauca和水忍冬L.dasystyla新鲜花蕾为材料,利用RT-PCR技术克隆获得了4种金银花类药用植物IspE和IspH基因的全长cDNA.运用生物信息学分析软件,预测编码蛋白的结构和功能,并通过RT-PCR检测IspE和IspH在忍冬、红白忍冬、红腺忍冬、水忍冬花蕾中的转录情况.结果:金银花类药用植物IspE基因含有1个完整的开放阅读框,长度为1 221 bp,编码406个氨基酸;IspH含有一个完整的开放阅读框,长度为1 380 bp,编码459个氨基酸.IspE和IspH均为非分泌蛋白,均定位于叶绿体中.RT-PCR分析结果表明在忍冬、红腺忍冬和水忍冬的花蕾中IspE,IspH基因的转录水平没有显著差异,但红白忍冬花蕾中IspE,IspH基因的转录水平均显著高于忍冬.结论:克隆获得忍冬、红白忍冬、红腺忍冬和水忍冬中IspE,IspH基因,并证实了其在不同金银花类药用植物中的表达,为进一步研究IspE,IspH基因对萜类化合物生物合成和花香气以及颜色的影响奠定了基础.  相似文献   

19.
湖北贝母为传统中药,然而《Flora of China》将其基原植物湖北贝母Fritillaria hupehensis归并于天目贝母F.monantha项下。该实验采用分子系统学方法,以川百合Lilium davidii为外类群,用核基因ITS序列和叶绿体基因rpl16序列、matK序列等3个片段对湖北贝母及其近缘类群天目贝母F.monantha、安徽贝母F.anhuiensis等进行联合建树分析,对湖北贝母植物的系统位置进行了探讨,为湖北贝母药材的安全使用提供分子证据。结果显示,分子系统树上,3种贝母各自的居群聚为一支,之后天目贝母与安徽贝母聚为一支,最后与湖北贝母聚为一支。表明湖北贝母与天目贝母的亲缘关系可能要远于安徽贝母与天目贝母之间的关系,因此不适宜将湖北贝母归并于天目贝母。  相似文献   

20.
中国石斛属植物文献计量研究   总被引:5,自引:2,他引:3  
石斛是珍稀濒危中药材,目前正处于快速发展阶段。为全面了解我国石斛属植物研究的历史和发展现状,作者以1954~2010年"中国知网中国学术期刊网络出版总库"收录的石斛研究文献为依据,采用文献计量学的原理和方法,对我国石斛属植物研究文献从文献年代分布、期刊分布与被引频率、主题分布、研究对象分布、作者与研究机构分布等方面进行了统计与分析。结果表明,我国石斛研究明显分为起步(2个)、停滞、平稳发展、快速上升5个阶段;期刊分布存在离散性与集中性并存的现象,已形成核心期刊研究群,并以《中国中药杂志》、《中草药》和《陕西中医》为代表;研究主题广泛涉及临床与药理、组织培养与种苗繁育、成分分析等多个领域,已经形成比较稳定的研究机构和团队,但研究对象差异显著,以铁皮石斛、金钗石斛和霍山石斛最为集中。我国石斛属植物的研究已取得显著成果,但种植产业发展缓慢,供需矛盾突出,预计种苗繁育与人工种植、产品开发、化学与药理等方面是未来的研究热点,其文献报道仍将进一步上升。  相似文献   

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