溃结灵对溃疡性结肠炎大鼠Th17分化的调节机制研究

目的:探讨溃结灵对溃疡性结肠炎(UC)大鼠Th17细胞分化相关因子的影响,从而揭示其作用机制。方法:采用雄性SD大鼠制作由三硝基苯磺酸(TNBS)所导致的溃疡性结肠炎模型,溃结灵高、中、低剂量以及柳氮磺胺吡啶(SASP)对溃疡性结肠炎大鼠给予治疗,治疗结束后采集结肠粘膜,采用酶联免疫吸附(ELISA)法检测大鼠结肠粘膜中白细胞介素6(IL-6)、转化生长因子β1(TGF-β1)的含量;采用荧光定量PCR检测大鼠结肠组织IL-6、TGF-β1、孤独核受体t(RORγt)、信号转导子与转录激活子3(STAT3)mRNA的表达;采用Western blot法检测结肠粘膜RORγt、STAT3蛋白表达。结果:溃结灵高剂量(18.3g/kg)和中剂量(9.2g/kg)可明显减少溃疡性结肠炎大鼠结肠的溃疡个数和溃疡面积。大鼠结肠粘膜中,模型组IL-6的含量及mRNA表达明显高于正常组,溃结灵高剂量组(18.3g/kg)和柳氮磺胺吡啶组(0.5g/kg)低于模型组;模型组TGF-β1的含量及mRNA表达明显低于正常组,溃结灵高剂量(18.3g/kg)和柳氮磺胺吡啶组(0.5g/kg)高于模型组。模型组结肠粘膜中RORγt、STAT3蛋白表达及mRNA表达明显高于正常组,溃结灵高(18.3g/kg)、低(4.6g/kg)剂组和柳氮磺胺吡啶组(0.5g/kg)的蛋白表达及mRNA表达低于模型组。结论:溃结灵可能通过调节溃疡性结肠炎大鼠结肠粘膜内与Th17分化相关的IL-6、TGF-β1、RORγt、STAT3的表达,从而抑制炎症反应,治疗溃疡性结肠炎。     

溃结安康汤对溃疡性结肠炎大鼠结肠组织IL-1β的作用及蛋白表达的影响

目的:研究溃结安康汤对溃疡性结肠炎大鼠白介素(IL)影响及其作用机制。方法:健康Wistar大鼠,随机分为空白对照组、模型组、补脾益肠丸组、SASP(柳氮磺胺吡啶)组、溃结安康汤组。TNBS/乙醇法建立溃疡性结肠炎大鼠模型。分组灌胃治疗后,检测溃结大鼠结肠组织IL-1β含量和IL-1β蛋白表达。结果:模型组与正常组比较,IL-1β含量升高、蛋白表达升高,具有统计学意义(P<0.05);补脾益肠丸组、SASP组、溃结安康汤组与模型组比较IL-1β含量下降、蛋白表达下降,具有统计学意义(P<0.05);溃结安康汤组与补脾益肠丸组、SASP组比较,IL-1β含量下降明显、蛋白表达下降明显,具有统计学意义(P<0.05)。结论:溃结安康汤可以降低溃疡性结肠炎大鼠模型中结肠组织IL-1β含量和蛋白表达。     

溃结安康汤对溃疡性结肠炎大鼠结肠组织氧自由基影响的实验研究

目的:研究溃结安康汤对溃疡性结肠炎大鼠结肠组织氧自由基影响及其作用机制。方法:健康Wist-ar大鼠,随机分为空白对照组、模型组、补脾益肠丸组、SASP组、溃结安康汤组。TNBS/乙醇法建立溃疡性结肠炎大鼠模型。分组灌胃治疗后,检测溃结大鼠结肠组织氧自由基MDA含量和SOD活性。结果:模型组与正常组比较,大鼠结肠组织中的MDA含量明显升高,SOD活性明显降低,具有统计学意义(P<0.05);补脾益肠丸组、SASP组、溃结安康汤组与模型组比较,MDA含量明显下降,SOD活性明显增强,具有统计学意义(P<0.05);溃结安康汤组与补脾益肠丸组、SASP组比较,MDA含量下降,SOD活性明显增强,具有统计学意义(P<0.05)。结论:溃结安康汤可以降低溃疡性结肠炎大鼠结肠组织MDA含量、增加SOD活性。     

慢结灵胶囊治疗冰醋酸诱发大鼠溃疡性结肠炎机理研究

目的:研究慢结灵胶囊治疗冰醋酸诱发大鼠溃疡性结肠炎的作用.方法:用番泻叶造成大鼠脾虚模型,再用冰醋酸法复制大鼠溃疡性结肠炎模型.结果:慢结灵胶囊1.16、0.58、0.29g/kg灌胃给药,明显降低醋酸型溃疡性结肠炎大鼠结肠病变程度,明显降低大鼠血和结肠粘膜中MDA、NO含量,明显提高大鼠血和结肠粘膜中SOD活性;明显降低大鼠血中TNF含量,增加IL-2含量.结论:慢结灵胶囊有治疗冰醋酸诱发大鼠溃疡性结肠炎作用,其机理与降低MDA、NO、TNF含量,提高IL-2含量和SOD活性,从而减轻对大鼠结肠粘膜的损害等有关.     

优化溃结方对溃疡性结肠炎大鼠模型结肠组织表皮生长因子影响的实验研究

目的:研究优化溃结方对溃疡性结肠炎大鼠表皮生长因子影响及其作用机制。方法:健康SD大鼠,随机分为空白对照组、模型组、补脾益肠丸组、SASP(柳氮磺胺吡啶)组、优化溃结方组。TNBS/乙醇法建立溃疡性结肠炎大鼠模型。分组灌胃治疗后,检测溃结大鼠结肠组织EGF含量。结果:模型组与正常组比较,EGF含量明显下降,具有统计学意义(P<0.05)。优化溃结方组、补脾益肠丸组、SASP组与模型组比较,EGF含量显著升高,具有统计学意义(P<0.05)。优化溃结方组与补脾益肠丸组比较,EGF含量升高,具有统计学意义(P<0.05)。优化溃结方组与SASP组比较,EGF含量升高,具有统计学意义(P<0.05)。结论:优化溃结方可以提高溃疡性结肠炎大鼠模型中结肠组织EGF的含量。     

溃结安康汤对溃疡性结肠炎大鼠MDA影响的研究

[目的]研究溃结安康汤对溃疡性结肠炎大鼠MDA影响及其作用机制。[方法]健康Wistar大鼠,随机分为空白对照组、模型组、补脾益肠丸组、SASP组、溃结安康汤组。TNBS/乙醇法建立溃疡性结肠炎大鼠模型。分组灌胃治疗后,检测溃结大鼠结肠组织MDA含量。[结果]模型组与正常组比较,MDA含量升高,具有统计学意义(P<0.05);补脾益肠丸组、SASP组、溃结安康汤组与模型组比较,MDA含量下降,具有统计学意义(P<0.05);溃结安康汤组与补脾益肠丸组、SASP组比较,MDA含量明显下降,具有统计学意义(P<0.05)。[结论]溃结安康汤可以降低溃疡性结肠炎大鼠模型中结肠组织MDA含量。     

溃结灵对溃疡性结肠炎大鼠肠道NLRP3炎性体的调控研究

目的:观察三硝基苯磺酸(TNBS)溃疡性结肠炎大鼠病理形态、结肠黏膜NLRP3炎性体蛋白表达、mRNA及其相关因子IL-18、IL-33的表达,从而揭示其可能的作用机制。方法:采用三硝基苯磺酸(TNBS)复制溃疡性结肠炎模型,60只大鼠随机分为6组,即正常组,模型组,柳氮磺砒啶(SASP)阳性对照组,溃结灵高、中、低剂量组。连续治疗10天后,采集粘膜样本。观察大鼠病理形态学变化,采用蛋白质印迹法和荧光定量PCR检测大鼠结肠组织NLRP3、ASC、caspase-1蛋白和mRNA的表达;ELISA法检测大鼠结肠粘膜中IL-18、IL-33的含量。结果:模型组大鼠出现不同程度的体重减轻、结肠缩短和结肠质量增加,病理结果显示固有层崩解,出血、溃疡形成,毛细血管扩张、充血,肌层及外膜内大量急慢性炎症细胞浸润。溃结灵高(18.3 g/kg)中(9.2g/kg)低(4.6g/kg)剂量组和柳氮磺砒啶组(0.5 g/kg)上述情况较模型组明显减轻。与正常组比较,模型组NLRP3、caspase-1、ASC的蛋白和mRNA表达显著增高;与模型组比较溃结灵高(18.3 g/kg)中(9.2g/kg)低(4.6 g/kg)剂量组、柳氮磺砒啶组(0.5 g/kg)NLRP3、caspase-1、ASC mRNA表达均显著减少,溃结灵高(18.3 g/kg)中(9.2g/kg)剂量组NLRP3、caspase-1、ASC的蛋白表达均显著减少,溃结灵低(4.6 g/kg)剂量组NLRP3、caspase-1、ASC的蛋白表达有下降趋势但无统计学意义;与正常组比较,模型组IL-18、IL-33的表达水平明显升高,溃结灵高(18.3 g/kg)中(9.2g/kg)低(4.6 g/kg)剂量组和柳氮磺砒啶组(0.5 g/kg)IL-18、IL-33的含量明显低于模型组。结论:溃结灵治疗UC的作用机制可能与抑制NLRP3炎性体各组分蛋白和mRNA表达的表达,并下调结肠黏膜IL18、IL33的含量,使得炎症缓解从而发挥治疗溃疡性结肠炎的作用。     

溃结灵对溃疡性结肠炎大鼠结肠粘膜IKK-α蛋白表达的作用

目的：观察溃结灵对溃疡性结肠炎（UC）大鼠结肠粘膜IKK-α蛋白表达的作用,对其作用机制进行初步探讨。方法：采用三硝基苯磺酸（TNBS）法制作UC大鼠模型,大鼠随机分为六组：正常对照组、模型对照组、溃结灵低、中、高剂量组、SASP阳性对照组。治疗10 d后处死大鼠取结肠组织,采取免疫组化（IHC）法检测IKK-α的阳性细胞表达率。结果：模型组结肠粘膜IKK-α蛋白阳性细胞表达率明显高于正常对照组（P〈0.01）,溃结灵中剂量组结肠粘膜IKK-α蛋白阳性细胞表达率明显低于模型组（P〈0.05）,溃结灵高剂量组和SASP阳性对照组结肠粘膜IKK-α蛋白阳性细胞表达率明显低于模型组（P〈0.01）。结论：溃结灵使TNBS法UC大鼠模型结肠粘膜IKK-α蛋白阳性细胞表达率明显降低,这可能是其治疗UC作用的机理之一。     

溃结宁合剂对实验性结肠炎大鼠结肠组织Caspase-3凋亡的影响

目的观察溃结宁合剂对大鼠溃疡性结肠炎(UC)的治疗作用及对结肠组织Caspase-3凋亡的影响,探讨其防治UC的作用机制。方法采用2,4-二硝基氯苯(DNCB)和醋酸复合法制备模型,将实验大鼠随机分为5组:正常对照组、模型对照组、阳性药(柳氮磺吡啶片,SASP)对照组、溃结宁合剂高剂量组、溃结宁合剂低剂量组。以各组实验大鼠结肠大体形态损伤评分、组织学损伤评分、疾病活动指数、Caspase-3等为检测指标。结果模型组大鼠结肠大体形态损伤评分、组织学损伤评分表明模型复制成功,溃结宁合剂明显改善上述指标,并显著降低Caspase-3的表达。结论溃结宁合剂对大鼠UC疗效显著,降低Caspase-3表达、延缓结肠上皮细胞凋亡可能是其治疗机制之一。     

溃结宁合剂对实验性结肠炎的作用机制探讨

目的:观察溃结宁合剂对大鼠溃疡性结肠炎(UC)的治疗作用,并探讨其机制。方法:采用2,4-二硝基氯苯(DNCB)和醋酸复合法制备模型,将实验大鼠随机分为5组:正常对照组、模型对照组、阳性药(柳氮磺吡啶片,SASP)对照组、溃结宁合剂高剂量组、溃结宁合剂低剂量组。以各组实验大鼠结肠大体形态损伤评分、组织学损伤评分、结肠湿重、肠重指数、基质金属蛋白酶-2、9(MMP-2、MMP-9)等为检测指标。结果:模型组大鼠结肠大体形态损伤评分、组织学损伤评分表明模型复制成功,溃结宁合剂明显改善上述指标,并显著降低MMP-2、MMP-9的表达。结论:溃结宁合剂通过抑制MMP-2、MMP-9活性,阻止细胞外基质成分过度降解,减轻结肠组织损伤,对UC有显著的保护作用。     

基于ICP-MS法的加味左金丸中无机元素分析

目的 通过电感耦合等离子体质谱（inductively coupled plasma mass spectrometry,ICP-MS）法建立加味左金丸中Cd、Pb、As、Hg、Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法。方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中⁷Li、²³Na、²⁴Mg、²⁷Al、⁴⁰Ca、⁴⁸Ti、⁵¹V、⁵²Cr、⁵⁵Mn、⁵⁶Fe、⁵⁸Ni、⁵⁹Co、⁶³Cu、⁶⁶Zn、⁷⁰Ga、⁷⁵As、⁷⁷Se、⁸⁶Sr以⁷²Ge作为内标;⁹⁵Mo、¹¹⁴Cd、¹¹⁸Sn、¹²¹Sb、¹³⁷Ba以¹¹⁵In作为内标;²⁰²Hg、²⁰⁵Tl、²⁰⁸Pb以²⁰⁹Bi作为内标。对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析。通过ICP-MS法进行测定。结果 26种无机元素线性的相关系数r ≥ 0.999 6,检出限为0.001~1.500μg/L,定量限为0.01~5.00 μg/L,精密度与重复性试验的RSD均小于5%,平均回收率在82.64%~106.44%,RSD均小于5%。对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、Ca、Fe 4种元素的含量比较高,均大于500 μg/g,Cd、Pb、As、Hg、Co、Li、Sb、Mo、Sn、Cr、Se、Tl的含量比较低,均小于1 μg/g。由结果可知,人体的常量元素,如Na、Mg、Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低。根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定。结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定。     

Erstaunliches Ergebnis einer Schädelakupunktur nach Yamamoto bei Hemihypsarrhythmie rechts

A girl, three and a half years old at the respective date, premature in 25th week of gestation, had been presented. On day three after birth, she had had a IVH III° right and left with severe involvement of parenchyma and other multiple complications, e. a. infant respiratory distress syndrome up to the 42nd day postpartum, and epileptic attacks with apnoeic events.     

七味红花殊胜散对缺血再灌注肝损伤的保护作用

目的::研究七味红花殊胜敬对缺血再灌注肝损伤的保护作用.方法:在大鼠肝缺血再灌注模型上,观察七味红花殊胜散对缺血再灌注肝损伤引起的血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)含量的变化.结果:七味红花殊胜散对大鼠血清MDA、SOD、GSH与再灌组比较有明显差异(P<0.05),NO含量与再灌组比较有明显差异(P<0.05).结论:七味红花殊胜散对大鼠急性缺血再灌注肝损伤具有保护作用.     

Methodology of Canine/Feline YNSA and Tail Acupuncture

BackgroundYamamoto New Scalp Acupuncture (YNSA) is a well-known acupuncture system for human patients which was developed by Dr. Yamamoto.ObjectiveMy aim was to transpose the human YNSA map onto canines and felines.MethodI started researching a canine/feline YNSA map in 2002. I investigated approximately 3,000 canines and felines for YNSA and 2,500 for Tail Acupuncture. The ratio of canines and felines was 6 to 4. I researched and found all the equivalent points by palpating animal patients on the basis of an exact Neck Diagnosis.ResultsI nearly completed the map in 2006. The points in the occipital region are incomplete and my research is still in progress. In the process of researching canine/feline YNSA points, I accidentally found the other microsystems around the tail and named it “Tail Acupuncture”.ConclusionsYNSA and Tail Acupuncture are acupuncture systems utilizing microsystems and support each other in canines and felines. The merits of YNSA and Tail Acupuncture are 1) immediate effects, such as in cases of lameness, 2) simplicity in finding the exact points to treat and 3) the scalp and the tail can be utilized separately or together as treatment zones, according to the situation.     

Chinesische Orthopädie: Teil 1: Die Zukunft der Akupunktur in der Postmoderne

The locomotor system is one of the major fields of application for acupuncture. The results of the large acupuncture studies funded by German Health Insurances from 2000 until 2006 are impressive and repressive: they restrict acupuncture application by allocation of reimbursement and create the necessity of reorientation, since acupuncture can neither be adequately described nor prescribed solely on the basis of disease entities. Regarding these disease entities, it seems rather necessary to analyse the layers of problems and formulate a hierarchy of therapeutic goals instead. Only in this way the effects of acupuncture, related techniques and further therapeutic modalities can be matched with the set of objectives. The article presents general principles and follows their application from biological aspects to implications for the locomotor system and consequences for the application of physical therapy. The manual of acupuncture focuses on therapeutic effects and objectives first, and then goes on to sketch simple therapeutic strategies and finally complex approaches within acupuncture. These may encompass acupuncture, microsystem acupuncture and complex therapeutic modes.     

Fallbericht: Patientin mit Schlafstörung

Background

Since the onset of her menopause about ten years ago, a sixty-year old patient has been suffering from hot flashes and sleep disorders causing her to become increasingly anxious and nervous. As the patient's mother had died of breast cancer, substitution of hormones was not advisable. Relaxation methods such as Yoga and Autogenic Training could only mildly alleviate the symptoms.

Objective

To effectively treat the sleep disorder, improve quality of life, lessen frequency and severity of hot flashes

Methods

Weekly sessions with ear- and body acupuncture for ten weeks

Results

After the third acupuncture session the patient reported a decrease of nightly waking episodes but suffered from acute pain in the neck as well as shoulder region. After nine therapy sessions, the patient reported a 50% decrease of nightly waking episodes. The hot flashes, however, remained unchanged.

Discussion

In this case, acupuncture significantly improved the patient's sleeping disorder. It also reduced the symptoms of anxiety and nervousness. Other menopausal symptoms such as hot flashes and dryness of mucous tissues, could not be influenced.     

Patientin mit chronisch rezidivierenden Gelenks- und Wirbelsäulenschmerzen

Background

Acupuncture as a regulative therapy is well-suited for the treatment of functional, reversible disorders. Additionally, the WHO recommends its application for pain therapy in cases of arthritis and back pain due to various causes. This case study should clarify which paths to take in the case of a multi-morbid patient suffering from chronic pains due to polyarthrosis.

Objective

Pain reduction and improvement of quality of life as well as improvement of sleep quality.

Methods

Annual treatment series consisting of 10 sessions each, one session per week.

Results

short-term pain reduction before undergoing endoprosthetic surgery; considerable improvement of general status and sleep.

Conclusions

Acupuncture may bring temporal relief in cases of chronic pain due to osteoarthritis and should therefore be applied in combination with western medicine. Acupuncture is particularly well-suited to treat the co-emergent vegetative symptoms.     

常见证候荷瘤小鼠神经内分泌免疫组织基因转录的总体特征

目的揭示常见证候荷瘤小鼠神经-内分泌-免疫组织基因转录的总体特征。方法采用小鼠标准化四诊及辨证方法，及Gene Chip Mouse Exon1．0ST Array等技术，检测H22荷瘤小鼠早期邪毒壅盛（邪毒）和气虚、中期阳气虚、中晚期气阴阳虚等4个常见证候下丘脑、垂体、肾上腺、睾丸、脾脏、胸腺、肿瘤RNA的转录与剪接。结果正常昆明种雄性小鼠下丘脑、垂体、肾上腺RNA电泳的28S峰低于18S，而睾丸、脾脏、胸腺、肿瘤相反。肿瘤发生的早期，下丘脑、垂体、肾上腺基因表达模式发生显著改变，邪毒尤甚，出现了失代偿。下丘脑28SRNA迅速抬升并持续，气虚强于邪毒。垂体RNA总量降低，28S陡降，邪毒尤甚。肾上腺RNA电泳特征类似垂体，气虚与邪毒相近，中晚期气阴阳虚尤甚。睾丸变化不明显。随着病情发展，脾脏重量持续增加，而胸腺相反，重量持续下降，蛋白合成和糖代谢下降。肿瘤组织蛋白合成与糖代谢，邪毒大于气虚。以上7个组织与正常对照组相比，有9127个基因表达发生差异、51126个外显子剪接发生差异。结论神经-内分泌-免疫网络组织基因转录的差异是荷瘤小鼠不同证候内在重要的物质基础。