引流熊胆取样次数与胆红素含量关系的探讨

本文用重氮化方法对不同取样次数的引流熊胆进行了胆红素含量测定,发现棕熊、黑熊其胆红素含量均呈规律性变化,并随着取胆次数的增加而升高。     

狗胆,熊胆和猪胆中胆汁酸的含量测定

对狗胆、熊胆和猪胆中的总胆酸、去氧胆酸、游离胆酸进行了含量测定。结果表明：狗胆中的胆汁酸含量高于熊胆和猪胆，其中去氧胆酸超过熊胆近１倍，超过猪胆近１０倍。说明狗胆是一味极有开发价值的胆汁类中药材，为狗胆的应用提供了科学的理论依据。     

血清γ-谷氨酰转肽酶对淤胆型婴儿肝病的诊断价值

目的探讨血清γ-谷氨酰转肽酶(GGT)活性测定对淤胆型婴儿肝病的诊断价值。方法采集86例淤胆型肝病患儿血清,以80例正常婴儿为对照,常规生化检测,包括血液总胆红素(TBil)、直接胆红素(DBil)、丙氨酸转氨酶(ALT)、谷氨酸转氨酶(AST)、碱性磷酸酶(ALP)、总胆汁酸的活性,对其结果进行比较。结果淤胆型婴儿肝病组TBil增高明显,以DBil增高为主。TBil平均值为176.04μmol/L、DBil平均值为106.42μmol/L,平均DBil值占平均TBil值的60.45%。正常对照组血清GGT测定结果为(26.64±15.92)IU/L,淤胆型婴儿肝病组为(224.53±177.32)IU/L,显著高于正常对照组(P<0.01),异常率为92%。淤胆型婴儿肝病GGT水平与血清中TBil、ALP和TBA水平有一定程度的相关性。结论血清GGT测定对淤胆型婴儿肝病的辅助诊断具有十分重要的意义,具有较高的特异性和敏感性。     

活熊取胆胆汁的研究

本文报道了活熊取胆胆汁的生药特征,并采用GC-MS-计算机联用仪测定活熊取胆胆汁与商品熊胆水解物中胆汁酸的种类及含量,通过比较确认活熊取胆胆汁代替商品熊胆的可行性。     

引流熊胆对大鼠实验性胃溃疡超微结构的影响

熊胆为熊科动物黑熊或棕熊的干燥胆汁。习以杀熊取胆,但近年国内广泛采用导管活体引流熊胆汁再经干燥而制得,称引流熊胆。熊胆具有清热、镇静、平肝、明目、杀虫之功效。用于治疗热黄、暑泻、小儿惊痫、疳疾、蛔虫痛、目翳、喉痹、鼻蚀、疔痔恶疮等症［２,３,４］。但目前为止对抗胃溃疡尚不清楚。本文通过观察引流熊胆对胃溃疡超微结构的变化．进行了研究。１材料与方法实验选用Ｗｉｓｔａｒ大鼠４０只．体重１８８±５６ｇ雌雄不拘．随机分为常水对照组、甲氢咪胍组０．２ｇ／ｋｇ和引流熊胆小剂量组０．０５ｇ／Ｋｇ、中剂量组０．１…     

中西医结合治疗新生儿高胆红素血症60例总结

目的 :观察茵陈退黄汤治疗新生儿高胆红素血症的临床疗效。方法 :治疗组在西药治疗的基础上加服中药茵陈退黄汤 ,对照组采用单纯西药治疗。结果 :治疗组总有效率为 96 .7%,对照组总有效率为 85 %,X2 =4.70 ,P <0 .0 5。两组治疗前后血清总胆红素浓度差值分别为 2 43± 45 .2umol/L ,2 0 1± 48.6umol/L ,t=4.783,P <0 .0 1。两组血清胆红素恢复正常时间分别为 5 .30± 2 .4天 ,7.70± 3.5 0天 ,t=4.2 1,P <0 .0 1。结论 :中西医结合治疗新生儿高胆红素血症优于单纯西药治疗组。     

郁金治疗淤胆型肝炎的研究

目的探讨郁金及其他中药治疗淤胆型肝炎的临床疗效。方法将90例入选患者随机分为6组,均给予复方丹参、门冬氨酸钾镁静滴。在此基础上,B组加其他中药,C组加常规量郁金,D组加常规量郁金和其他中药,E组加大量郁金,F组加大量郁金和其他中药。6组疗程均为21 d。结果LSD多重比较显示,使用大量郁金与使用常规量及不使用郁金间对降低血清总胆红素均有非常显著性差异(P=0.001,P=0.000)。血清总胆红素下降均值由高到低依次为使用大量((164.3±116.4)μmol/L)、使用常规量((101.3±72.0)μmol/L)和不用((72.3±73.8)μmol/L)。其他中药用与不用对降低血清总胆红素有非常显著性差异(F=46.823,P=0.000),血清总胆红素下降均值由高到低依次为用((164.2±93.6)μmol/L)和不用((61.1±69.0)μmol/L)。结论郁金与其他中药均可降低血清总胆红素水平,大剂量郁金较常规量效果好,大剂量郁金与其他中药合用疗效最好,而且两者有协同作用。     

血清乙肝病毒定量与肝损伤及干扰素疗效的关系

 目的:探讨血清乙肝病毒定量与肝脏损伤及干扰素治疗效果的关系。方法:对34例乙肝e抗原（HBeAg）及乙肝表面抗原（HBsAg）阳性的乙肝患者进行干扰素治疗，对患者血清进行乙肝病毒定量测定及白蛋白（ALB）、白蛋白/球蛋白比值（A/G）、谷-丙转氨酶（ALT）、谷-丙转氨酶（AST）、总胆红素(TBIL)、直接胆红素(DBIL)测定，将血清病毒含量≥10⁷、106.9～105.1、≤10⁵分别定为高、中、低含量组。并于治疗前行肝脏组织病理检查。结果:34例患者血清HBV-DNA含量范围在10⁴～10⁹拷贝·mL^-1，其中13例高病毒含量患者，ALT值(156.0±21.3)IU·L^-1,AST值(145.0±11.0) IU·L^-1,S 值为(2.07±0.21),G值为(2.1±0.7)；14例中病毒含量患者,ALT值(285.0±18.0) IU·L^-1,AST值(125.0±55.8) IU·L^-1,S值为(2.0±0.4),G值为(2.4±0.4)；7例低病毒含量患者ALT值(167.0±21.3) IU·L^-1,AST值(128.0±27.7) IU·L^-1,S 值为(3.01±0.11),G值为(3.06±0.09)。3组数值之间无明显相关性(P＞0.05),予干扰素治疗24周后，高、中、低病毒含量组患者有1例(7.69%)，3例（21.43%），3例（47.86%）血清HBV DNA阴转，3者之间差异有显著性（P＜0.05)。结论:乙肝患者血清病毒含量与肝脏损伤程度无关，干扰素治疗效果与病毒含量呈正比关系，定量血清乙肝病毒DNA检测（HBV-DNA）是预测和评价干扰素治疗效果的可靠指标之一。     

掺血熊胆的鉴别

熊胆为熊科动物棕熊Ursus arctos L·或黑熊Selenarotos thibetanuscurier 的干燥胆。目前掺伪者甚多。最近笔者在检验儿批从内蒙、黑龙江等地购进的熊胆中发现有掺血熊胆。现将鉴别方法介绍如下: 一、性状鉴别掺血熊胆的性状与正品类似;但多饱满充实,胆仁黑褐色或类黑色硬膏状,有腥气,味淡不苦或微苦,无清谅钻舌感。二、理化鉴别 1.荧光正品应呈黄白色荧光;掺血熊胆的荧光为金黄     

猪胆盐及猪胆中熊脱氧胆酸的鉴定

用薄层色谱法从生物试剂-猪胆盐和6批猪胆中分离出了熊脱氧胆酸,并用IR,MS,NMR进行了鉴定,证明该成分不是唯独在熊胆中存在,只是熊胆中该成分的含量较高。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.     

瑞香科五属植物中酚性聚合物的结构特征、分布及药理活性研究进展

瑞香科植物广泛分布于世界热带和温带地区,其中多种植物可供药用。在对该科植物的研究中发现,黄酮类、木脂素和香豆素等酚性化合物的低聚体为其重要的特征性成分,药理活性主要表现在抗肿瘤、抗炎、抗氧化、抗菌和抗病毒等方面。对已发现的酚性聚合体的化学结构、植物来源及药理活性进行综述,为瑞香科植物今后的研究和开发利用提供参考。     

HPLC法同时测定沉香化滞丸中11种成分

目的采用HPLC梯度洗脱法同时测定沉香化滞丸中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚11种成分。方法采用Thermo Syncronis C18色谱柱(250 mm×4.6 mm,5μm),流动相为水-乙腈,梯度洗脱:0～10 min,20%乙腈;10～20 min,20%～40%乙腈;20～24 min,40%乙腈;24～26 min,40%～52%乙腈;26～30 min,52%乙腈;30～31 min,52%～90%乙腈;31～35 min,90%乙腈;35～40 min,90%～100%乙腈;40～43min,100%乙腈;43～45min,100%～20%乙腈;检测波长215nm,体积流量1.0m L/min,柱温30℃,进样量20μL。结果各成分在43 min内分离良好,沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚的线性范围分别为1.4～13.6、10.0～200.0、31.5～315.0、1.0～120.1、1.8～50.6、0.93～10.1、1.8～30.0、0.2～40.3、1.8～18.1、1.7～25.0、0.45～10.70μg/mL;样品中各成分的平均回收率均在98.90%～100.87%;11种成分精密度RSD在0.55%～1.54%;供试品溶液在30 h内稳定性良好,RSD在0.75%～1.94%;重复性RSD在0.39%～1.73%。6批次样品中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚质量分数分别为92.0～201.0、511.5～9 033.0、5 475.0～12 635.5、54.5～5 095.5、192.0～2 137.5、117.0～391.5、106.5～1 281.5、13.0～136.5、93.5～199.0、177.0～1 207.0、33.5～251.5μg/g。结论本方法准确、快速、简便,重复性好,精密度高,适用于沉香化滞丸中多种活性成分的定量分析。