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1.
目的:研究乌骨藤提取物对体外培养的人肠癌细胞(lovo)增殖的影响.方法:用两种方法(A、B)制备乌骨藤提取物,采用不同浓度的鸟骨藤提取物分别作用于培养的lovo细胞,通过细胞形态学和MTT法检测鸟骨藤提取物对lovo细胞增殖的影响.结果:乌骨藤提取物A对细胞的最大无毒浓度大约为0.648mg/ml;药物浓度在0.389mg/ml、0.648mg/ml、1.08mg/ml、1.8mg/ml、3mg/ml时的抑制率分别为6.58%、8.24%、13.02%、59.69%、66.23%;用回归法求出鸟骨藤提取物A对lovo细胞增殖的半数抑制浓度为1.625mg/ml.乌骨藤提取物A对细胞的最大无毒浓度大约为0.648mg/ml;药物浓度在0.389mg/ml、0.648mg/ml、1.08mg/ml、1.8mg/ml、3mg/ml时的抑制率分别为14.49%、15.48%、29.69%、63.26%、64.05%;用回归法求出乌骨藤提取物B对lovo细胞增殖的半数抑制浓度为1.84mg/ml.结论:乌骨藤提取物对体外培养的人肠癌细胞(lovo)的增殖有明显抑制作用.  相似文献   

2.
川芎嗪含药血清对人肝癌细胞HepG2增殖的抑制作用   总被引:6,自引:0,他引:6  
丰俊东  徐晓玉 《中草药》2005,36(4):551-553
目的观察川芎嗪(TMP)含药血清对人肝癌细胞HepG2增殖的影响。方法选用SD雄性大鼠30只,随机分成3组,分别ipTMP143.0、71.5mg/kg、生理盐水0.8mL,制备TMP大、小剂量组及生理盐水组含药血清,采用RP-HPLC法测定含药血清中TMP的质量浓度。将各组含药血清作用于人肝癌细胞HepG248h,MTT法测定不同质量浓度TMP含药血清对人肝癌细胞HepG2增殖的抑制作用。结果TMP大剂量组血清(20%、10%、5%),TMP小剂量组血清(20%、10%)对人肝癌细胞HepG2增殖较生理盐水组有显著抑制作用(P<0.05),最高抑制率可达46.1%。结论TMP含药血清对人肝癌细胞HepG2增殖有一定的抑制作用。  相似文献   

3.
目的:研究番荔枝种子内酯提取物对人肝癌细胞株HepG2的作用。方法:以血清药理学方法考察不同剂量的番荔枝种子内酯提取物含药血清对人肝癌细胞株HepG2的抑制作用,用MTT法进行评价。结果:内酯提取物含药血清作用于HepG2细胞的抑制率呈现出时间和剂量依赖性,高剂量组作用72h时抑制率可达54.16%。结论:番荔枝种子内酯提取物对体外培养的人肝癌HepG2细胞的生长增殖具有显著的抑制作用。  相似文献   

4.
目的 研究水龙骨对人肝癌细胞株HepG2、SMMC-7721的生长增殖抑制作用.方法 制备水龙骨含药血清,用MTT法观察细胞增殖情况.结果 水龙骨含药血清在体外对人肝癌细胞株HepG2、SMMC-7721均有抑制作用,对HepG2的最高抑制率为87.4%,对SMMC-7721的最高抑制率为85.9%,抑制肝癌细胞的效果优于含5-氟尿嘧啶血清.结论 水龙骨对肝癌细胞的体外增殖有明显的抑制作用.  相似文献   

5.
观察牛黄参胶囊对体外培养的人肝癌细胞HepG2的影响。方法制备牛黄参胶囊含药血清(NHS),MTT法检测不同浓度含药血清对肝癌细胞HepG2的影响。结果 20%浓度含药血清能明显抑制HepG2细胞增殖。结论 NHS含药血清对体外培养的人肝癌细胞HepG2具有抑制作用,能促进肝癌细胞HepG2的凋亡。  相似文献   

6.
肝必康胶囊体外抗乙肝病毒的实验研究   总被引:1,自引:0,他引:1  
目的:观察肝必康胶囊对乙肝病毒的抑制作用.方法:采用HepG 2.2.15细胞的培养,MTT比色法检测药物对细胞的毒性,定量PCR检测HBV-DNA,ELISA法检测HBsAg、HBeAg的含量,研究肝必康胶囊对HepG 2.2.15细胞的毒性,HBsAg与HBeAg表达及HBV-DNA含量的影响.结果:肝必康半数细胞毒浓度(TC50)为4.677 mg/mL,最大无毒浓度(TC0)为0.118 mg/mL.各组在用药后72、144 h细胞上清中HBV DNA拷贝数降低,肝必康在0.1 mg/mL时抑制率最高.各组用药后细胞培养上清液中的HBsAg和HBeAg浓度显著降低.肝必康在0.1 mg/mL时抑制率最高.肝必康各组含药血清在用药后72、144 h细胞上清中HBsAg和HBeAg浓度显著降低,高剂量组抑制率最高.显示明显抗HBV的作用,TI均大于2.结论:肝必康胶囊体外培养细胞实验证实其有较强的抗乙肝病毒作用,且毒性较低.  相似文献   

7.
目的:探讨藤茶总黄酮和二氢杨梅素含药血清对人肝癌细胞株HepG2增殖的抑制及凋亡的影响.方法:采用血清药理学方法制备藤茶总黄酮、二氢杨梅素、环磷酰胺(CTX)含药血清以及对照血清,作用于肝癌HepG2细胞,MTT法检测各种血清对HepG2细胞增殖的影响;倒置相差显微镜观察各种含药血清作用后肝癌HepG2细胞的形态学变化;AnnexinV/7AAD双标记法流式细胞术检测早期细胞凋亡的情况.结果:MTT法和显微镜观察显示藤茶总黄酮和二氢杨梅素20%含药血清能不同程度抑制HepG2细胞增殖,可致细胞形态学改变;AnnexinV/7AAD双标记法检测显示藤茶总黄酮20%含药血清可促肝癌HepG2细胞早期凋亡,但二氢杨梅素20%含药血清对肝癌HepG2细胞早期凋亡没有影响.结论:藤茶总黄酮能抑制肝癌HepG2细胞的增殖,诱导其产生早期凋亡.  相似文献   

8.
目的:研究双氢青蒿素含药血清抑制人肝癌SMMC-7721细胞增殖和诱导细胞凋亡的作用及作用机制。方法:制备空白组、双氢青蒿素15 mg/kg、30 mg/kg、60mg/kg、120 mg/kg 5个剂量组含药血清,以贝伐单抗为对照药,作用于人肝癌SMMC-7721细胞;噻唑蓝法检测各组细胞增殖;流式细胞仪检测各组细胞凋亡;制作细胞爬片,免疫组化法检测各组细胞的血管内皮生长因子表达。结果:和空白组比较,双氢青蒿素含药血清30 mg/kg组、60 mg/kg组、120mg/kg组能明显抑制SMMC-7721细胞增殖并诱导细胞凋亡。24h的增殖抑制率分别为22.41%、29.31%和36.21%,48h的增殖抑制率分别为24.68%、36.36%和41.56%,72h的增殖抑制率分别为30.59%、38.82%和45.88%;凋亡率分别为16.13%、17.42%和19.73;免疫组化结果显示各双氢青蒿素组细胞的血管内皮生长因子表达均受到明显抑制。结论:双氢青蒿素含药血清浓度达到30 mg/kg以上时表现出明显的抑制细胞增殖和诱导细胞凋亡作用,并且这种作用可能与其对血管内皮生长因子的表达抑制相关。  相似文献   

9.
目的探讨藤梨根提取物对肝癌细胞生物学特性的影响以及潜在作用机制。方法通过不同浓度藤梨根正丁醇提取物(0,25,50,100,200,400μg/mL)作用于人肝癌HepG2细胞。采用噻唑蓝(MTT)法检测肝癌HepG2细胞增殖情况,采用流式细胞术和Transwell法检测细胞凋亡、迁移、侵袭情况,采用Western blot法检测核因子-κB(NF-κB)信号通路蛋白表达水平。结果不同浓度藤梨根提取物对人肝癌HepG2细胞增殖有抑制作用(P均0.05),且具有浓度依赖性,其半数抑制浓度(IC50)为145.365μg/mL。145μg/mL的藤梨根提取物可显著抑制HepG2细胞迁移、侵袭(P0.05),诱导凋亡(P0.05),抑制NF-κB磷酸化(P0.05),下调基质金属蛋白酶-2(MMP-2)蛋白水平(P0.05)。结论藤梨根正丁醇提取物可能通过影响NF-κB信号通路调控肝癌细胞生物学特性。  相似文献   

10.
陈红  王维 《中草药》2019,50(9):2115-2120
目的研究红藤提取物联合5-氟尿嘧啶对人肝癌HepG2细胞生长的抑制作用,并初步探讨其作用机制。方法体外培养HepG2细胞,以不同质量浓度的红藤提取物作用于细胞,采用MTT法检测其对细胞生长的影响,选择后续实验浓度。将HepG2细胞分为对照组、红藤提取物(40 mg/L)组、5-氟尿嘧啶(10μmol/L)组及联合用药(红藤提取物40 mg/L+5-氟尿嘧啶10μmol/L)组,采用MTT法检测细胞增殖抑制率,流式细胞仪检测细胞周期,Western blotting法检测细胞中增殖细胞核抗原(PCNA)、细胞周期蛋白D1(Cyclin D1)及细胞周期依赖性蛋白激酶4(CDK4)表达水平。结果 MTT检测结果显示,红藤提取物呈质量浓度依赖性地抑制HepG2细胞增殖,选择其质量浓度为40mg/L用于后续实验。与对照组比较,红藤提取物组和5-氟尿嘧啶组细胞增殖抑制率显著升高,G_0/G_1期细胞比例显著升高,S期和G2/M期细胞比例显著降低,细胞中PCNA、Cyclin D1、CDK4蛋白的表达显著降低(P0.05)。与5-氟尿嘧啶组比较,联合用药能显著抑制HepG2细胞增殖,阻滞细胞周期,抑制细胞中PCNA、Cyclin D1、CDK4蛋白表达(P0.05)。结论红藤提取物联合5-氟尿嘧啶能够增强对HepG2细胞生长的抑制作用,其作用机制可能与抑制细胞中PCNA、Cyclin D1、CDK4蛋白表达水平有关。  相似文献   

11.
小柴胡汤对体外培养人肝癌细胞株HepG2增殖的影响   总被引:2,自引:0,他引:2  
目的探讨小柴胡汤45%乙醇提取物对体外培养人肝癌细胞株HepG2增殖的影响及其可能的机理。方法采用ATP-Lite法测定不同浓度小柴胡汤对体外培养的HepG2增殖的作用,同时利用氧自由基清除能(ORAC)法测定样品的抗氧化活性;以LPS刺激小鼠巨噬细胞RAW264.7产生一氧化氮(NO),Griess法测定NO的终产物亚硝酸盐的含量,观察样品对NO生成的影响。结果小柴胡汤在62.5~500mg/L浓度范围内可以显著抑制HepG2增殖,抑制作用呈剂量相关性,计算半数抑制浓度(IC50)为(90.7±23.7)mg/L。小柴胡汤提取物具有一定的抗氧化活性,其抗氧化能力为阳性对照维生素C的1.3倍。小柴胡汤在3.125~50mg/L浓度范围内均可以显著抑制NO的生成(P0.05,P0.01)。结论小柴胡汤具有体外抑制人肝癌细胞株HepG2增殖的作用,其抑制作用可能与其抗氧化活性及抑制NO生成有关。  相似文献   

12.
High-dose aqueous extracts from artichoke leaves were found to inhibit cholesterol biosynthesis from (14)C-acetate rather moderately in HepG2 cells in contrast to primary cultured rat hepatocytes in which the inhibition was stronger. Preincubation of the extracts with several glycohydrolases revealed that pretreatment with beta-glucosidase considerably reinforced the inhibition. A significant reduction of acetate incorporation was found above extract concentrations of 0.01 mg/mL and at 0.2 mg/mL almost 60% inhibition was observed. Cytotoxic effects detected by the MTT-assay were restricted to higher concentrations of the extracts with and without beta-glucosidase pretreatment. Since cynaroside represents a major glucoside in artichoke extracts, both cynaroside and its aglycone luteolin were tested. It could be demonstrated that cynaroside is indeed one of the targets of beta-glucosidase and that the liberated luteolin is responsible for the inhibitory effect. Direct measurements of beta-glucosidase activity in rat hepatocytes and HepG2 cells revealed that endogenous enzyme activity in hepatocytes may be sufficient to convert cynaroside to its aglycone, while in HepG2 cells this may not be the case. These findings emphasize the importance of beta-glucosidase-dependent liberation of luteolin for the ability of artichoke extracts to inhibit hepatic cholesterol biosynthesis.  相似文献   

13.
目的:探讨通关藤提取物(MTE)对人肝癌HepG2细胞凋亡的影响及诱导凋亡的途径。方法:采用不同浓度MTE(15、20、30 mg/mL)处理HepG2细胞,AnnexinV-FITC/PI双染法检测MTE对HepG2细胞凋亡的影响,JC-1染色法检测HepG2细胞线粒体膜电位,蛋白质印迹(Western blotting)技术检测HepG2细胞线粒体凋亡途径相关蛋白的表达变化。结果:不同浓度MTE可诱导HepG2细胞出现细胞凋亡,并不同程度降低HepG2细胞线粒体跨膜电位。MTE可下调B细胞白血病/淋巴瘤-2(Bcl-2)蛋白的表达,同时上调Bcl-2相关X蛋白(Bax)、细胞色素c(Cytochromec)、半胱氨酸蛋白酶9(Caspase-9)和Caspase-3的蛋白表达。结论:MTE可通过诱导HepG2细胞凋亡发挥抗肿瘤作用,其作用机制与内源性线粒体凋亡途径有关。  相似文献   

14.
目的研究龙胆泻肝汤对阴道加德纳菌(GV)的体外抑制作用。方法利用稀释法检测龙胆泻肝汤对GV增殖的影响,并测定药物的最低抑菌浓度,通过革兰氏染色法观察龙胆泻肝汤对GV黏附人宫颈癌Hela细胞的影响,MTT法检测龙胆泻肝汤对GV细胞毒性的影响,96孔微量板法检测龙胆泻肝汤对GV生物膜形成的影响,qRT-PCR法检测龙胆泻肝汤对GV BAP、sialidase mRNA表达的影响。结果龙胆泻肝汤水提物、70%醇提物和90%醇提物对GV的最低抑菌浓度分别为(62.5±3.6)、(15.6±1.5)、(125.0±2.8) mg/mL。龙胆泻肝汤70%醇提物(1.56、15.6 mg/mL)能降低GV对Hela细胞的黏附,抑制GV生物膜的形成(P<0.01),下调BAP、sialidase mRNA表达(P<0.05,P<0.01),龙胆泻肝汤70%醇提物(15.6 mg/mL)抑制GV对Hela细胞的毒性作用(P<0.01)。结论龙胆泻肝汤70%乙醇提物能显著抑制GV增殖,并能抑制GV细胞毒性、粘附能力和生物膜形成。  相似文献   

15.
Anisomeles indica (L.) Kuntze (Labiatae), is a traditional anti-inflammatory herb used in Taiwan. The aqueous and methanolic extracts of whole plants, leaves, flowers and stems; and chloroform and n-butanol fractions of methanol extract, from A. indica were investigated for their anti-inflammatory activity on murine peritoneal macrophages. In addition, the tumor cells proliferation inhibition activities of these extracts were also evaluated against a panel of tumor cell lines such as Colon 205, PC 3, HepG2 and MCF 7. Treatment with A. indica extracts did not reduce cell viability at any dose used. However, all the extracts significantly inhibited the enhanced production of NO radicals, and pro-inflammatory cytokines (TNF-alpha, and IL-12) induced by LPS/IFN-gamma in a dose-dependent manner. Furthermore, methanolic extracts of leaves and flowers significantly and dose-dependently arrest mitogen-stimulated spleen cells in G0/G1 stage, in addition to their cell proliferation inhibition against Colon 205, MCF 7 and PC 3 by 94, 82; 98, 71; 82, 98%, respectively, at 200 microg/mL concentration. This is the first report on A. indica extracts for their growth inhibitory activities, against inflammatory mediator production, and human tumor cell lines, colon, prostate, hepatoma and breast cells proliferation.  相似文献   

16.
红曲提取物对体外培养成骨细胞的作用研究   总被引:2,自引:0,他引:2  
目的:研究红曲不同提取物对体外培养大鼠成骨细胞(ROB)增殖、分化的影响。方法:采用新生大鼠颅骨分离培养ROB,MTT法测定细胞增殖,碱性磷酸酶(ALP)试剂盒法测定ALP活性,观察红曲提取物对ROB增殖、分化的作用,同时考察了MTT法和ALP试剂盒法中细胞数和吸收值之间的线性关系。结果:10^-2和10^-3mg/ml的水提物能刺激ROB的增殖(P〈0.05),10^-3mg/ml的水提物还能提高ROB的ALP活性(P〈0.05);10^-2和10^-3mg/ml的95%乙醇提取物对ROB的增殖与分化均有明显的促进作用(P〈0.05或P〈0.01)。结论:红曲水提物和95%乙醇提取物均能促进ROB骨形成过程中增殖、分化,95%乙醇提取物作用效果显著。  相似文献   

17.
槐果碱体外对HepG2.2.15细胞分泌HBsAg HBeAg的影响   总被引:4,自引:0,他引:4  
目的:探讨槐果碱的体外抗乙型肝炎病毒作用。方法:采用HepG2.2.15细胞模型进行体外培养,给予不同浓度槐果碱,以拉米夫定作阳性对照,作用9天后检测上清液中HBsAg、HBeAg的分泌,观察药物对HepG2.2.15细胞分泌HBV病毒抗原的影响,同时以MTT法检测药物在体外对HepG2.2.15细胞的生长抑制作用,从而评价药物的抗HBV作用。结果:药物作用9天后,槐果碱对HepG2.2.15细胞的50%生长抑制率(TC50)为0.002M,对HepG2.2.15细胞分泌HBsAg的50%抑制率(IC50)为4.9uM,其治疗指数(TI=TC50/IC50)为408.16;而拉米夫定对HepG2.2.15细胞分泌HBsAg的抑制率在所选浓度范围内均低于50%。槐果碱对HepG2.2.15细胞分泌HBeAg的抑制率在所选浓度范围内均低于50%,但明显优于拉米夫定对HepG2.2.15细胞分泌HBeAg的抑制率。结论:槐果碱在体外具有显著的抑制HepG2.2.15细胞分泌HBsAg、HBeAg的作用,是一个高效低毒的抗乙肝病毒的有效药物。  相似文献   

18.
目的:运用小鼠急性毒性试验和基于Microtox(微毒)技术的生物综合毒性评价技术对比研究金银花醇提物和山银花醇提物的毒性大小,探索Microtox技术应用于中药毒性快速评价的可行性。方法:小鼠一次性灌胃给予不同浓度的金银花醇提物、山银花醇提物,观察并记录小鼠的毒性反应及死亡情况,计算小鼠的半数致死量(LD50);利用Microtox技术测定金银花醇提物、山银花醇提物对发光菌发光强度抑制率,计算发光细菌半数抑制浓度(IC50),将2种毒性评价方法的结果进行比较。结果:小鼠灌胃金银花醇提物和山银花醇提物的半数致死量(LD50)值分别为81.29 g生药/kg、91.0 g生药/kg。采用生物综合毒性评价技术测得对发光细菌的半数抑制率分别为0.001 3 g生药/mL、0.002 2 g生药/mL。结论:应用小鼠急性毒性试验及基于Microtox技术的生物综合毒性评价测试金银花提取物和山银花提取物,2种评价结果具有一定的一致性,将Microtox技术用于金银花提取物和山银花提取物毒性的快速评价具有一定的可行性,但2种毒性评价方法的相关程度还有待扩大样本量进一步研究。  相似文献   

19.
The cytotoxicity of extracts from a widely used species of plant, Moringa stenopetala, was assessed in HEPG2 cells, by measuring the leakage of lactate dehydrogenase (LDH) and cell viability. The functional integrity of extract-exposed cells was determined by measuring intracellular levels of ATP and glutathione (GSH). The ethanol extracts of leaves and seeds increased significantly (p < 0.01) LDH leakage in a dose- and time-dependent manner. The water extract of leaves and the ethanol extract of the root did not increase LDH leakage. A highly significant (p < 0.001) decrease in HEPG2 viability was found after incubating the cells with the highest concentration (500 microg/mL) of the ethanol leaf and seed extracts. At a concentration of 500 microg/mL, the water extract of leaves increased (p < 0.01), while the ethanol extract of the same plant part decreased (p < 0.01), ATP levels. The root and seed extracts had no significant effect on ATP levels. The ethanol leaf extract decreased GSH levels at a concentration of 500 microg/mL (p < 0.01), as did the ethanol extract of the seeds at 250 microg/mL and 500 microg/mL (p < 0.05). The water extract of the leaves did not alter GSH or LDH levels or affect cell viability, suggesting that it may be non-toxic, and is consistent with its use as a vegetable. The data obtained from the studies with the ethanol extract of the leaves and seeds from Moringa stenopetala show that they contain toxic substances that are extractable with organic solvents or are formed during the process of extraction with these solvents. The significant depletion of ATP and GSH only occurred at concentrations of extract that caused leakage of LDH. Further investigation with this plant in order to identify the constituents extracted and their individual toxic effects both in vivo and in vitro is warranted. This study also illustrates the utility of cell culture for screening plant extracts for potential toxicity.  相似文献   

20.
The methanol extract from Morinda citrifolia fruits was tested for cytotoxicity activity on the MTT assay. The appearance of cytotoxic changes after exposure to the extract was in a concentration dependent manner. The median lethal concentrations (LC(50)) of the extract in baby hamster kidney (BHK) cells, African green monkey kidney (Vero) cells and human laryngeal carcinoma (Hep2) cells were found to be 2.5, 3 and 5 mg/mL, respectively. A concentration of 0.1 mg/mL of crude extract exhibited cytotoxic activity against breast cancer (MCF7) and neuroblastoma (LAN5) cell lines at 29% and 36%, respectively. The same concentration of extract showed no toxicity to Vero and very little toxicity to BHK (6%) and Hep2 (13%) cells.  相似文献   

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