炒椿皮配方颗粒HPLC特征图谱及含量测定方法的建立

目的 建立炒椿皮配方颗粒高效液相色谱法（HPLC）特征图谱及含量测定方法。方法 采用HPLC,色谱柱为资生堂MGⅡ C₁₈（250 mm×4.6 mm,5 μm）,以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速为1.0 mL·min^–1,柱温为30 ℃,检测波长为254 nm,进样量为10 μL,以铁屎米酮为参照,建立炒椿皮配方颗粒的特征图谱,利用对照品对其共有峰进行指认,并对炒椿皮饮片、标准汤剂和配方颗粒的相关性进行评价。同时以铁屎米酮为对照品,以乙腈-0.1%磷酸水溶液（29∶71）为流动相,检测波长为360 nm,进样量为20 μL,建立其含量测定方法。结果 所建立的炒椿皮配方颗粒HPLC特征图谱共确定了9个特征峰,指认了其中3个,各样品特征图谱与对照图谱相似度为0.984~1.000。含量测定结果表明,铁屎米酮质量浓度为1.018~12.216 μg·mL^–1时与峰面积呈良好线性关系;铁屎米酮平均加样回收率为99.22%,RSD<2%;10批炒椿皮配方颗粒中铁屎米酮质量分数为0.209 7~0.215 9 mg·g^–1。结论 建立的特征图谱及含量测定方法操作简便、专属性强、重复性好,可为炒椿皮配方颗粒的质量控制提供参考。     

瓜蒌皮药材的HPLC指纹图谱

目的：建立瓜蒌皮药材的 HPLC 指纹图谱。方法：采用 Agilent TC-C₁₈（4.6 mm×250 mm,5 μm）色谱柱,以乙腈-0.2%冰乙酸为流动相进行梯度洗脱,检测波长 260 nm,流速 0.8 mL·min^-1,柱温 25℃,进样20 μL。结果：建立了瓜蒌皮药材的HPLC指纹图谱,方法学考察结果良好,确立了13个共有峰,其中5个共有峰得到确认,10批瓜蒌皮样品指纹图谱的相似度均>0.9。结论：该方法稳定性、重复性好,建立的指纹图谱可为瓜蒌皮的质量评价提供依据。     

桃枝类药材HPLC指纹图谱的鉴别

目的: 建立桃枝类药材高效液相色谱(HPLC)指纹图谱鉴别方法。方法: 采用RP-HPLC,Diamonsil-C₁₈(4.6 mm×250 mm,5 μｍ)色谱柱,流动相为乙腈-0.05%磷酸水,梯度洗脱,流速0.5 mL·min^-1,290 nm波长下测定山桃枝、桃枝、桃叶及桃仁指纹图谱,并作相似度比较分析。结果: 建立了山桃枝药材HPLC指纹图谱共有模式,有47个共有指纹峰被标定,并对山桃枝、桃枝、桃叶及桃仁药材的HPLC指纹图谱进行相似度比较,结果有明显差异。结论: HPLC指纹图谱具有方法简便,重复性好,特征性强的特点,可用于桃枝类药材鉴别。     

脱脂炒莱菔子提取物HPLC指纹图谱

目的：建立脱脂炒莱菔子提取物的HPLC指纹图谱。方法：依利特C₁₈柱（4.6 mm×250 mm,5 μm）,流动相乙腈（A）-0.1%磷酸水溶液（B）,梯度洗脱（0~28 min,5%~30%A;28~30 min,30%~70%A;30~51 min,70%~88%A）,流速1.0 mL·min^-1,检测波长225 nm,柱温30 ℃。结果：建立了脱脂炒莱菔子提取物的HPLC指纹图谱,共标示出10个共有峰;不同产地脱脂炒莱菔子提取物的HPLC指纹图谱相似度>0.96。结论：该方法准确稳定,重复性好,可为脱脂炒莱菔子提取物的定性鉴别提供依据。     

桃枝HPLC指纹图谱的研究

目的：建立桃枝的HPLC指纹图谱,评价山桃枝、桃枝、桃叶及桃仁HPLC图谱的相似性。方法：以HPLC为检测手段,采用Diamonsil-C₁₈（4.6 mm×250 mm,5 μm）色谱柱,流动相乙腈-0.05%磷酸水,梯度洗脱,流速0.5 mL·min^-1,波长295 nm为色谱条件进行测定。结果：建立了山桃枝HPLC指纹图谱共有模式,标定了23个共有峰,对山桃枝、桃枝、桃叶及桃仁的HPLC指纹图谱进行相似度比较。结论：该方法简便、准确、重复性好,可作为桃枝品质鉴定的依据。     

怀地黄药材HPLC指纹图谱研究

目的: 建立怀地黄药材的HPLC指纹图谱,为科学评价及有效控制其质量提供可靠方法。 方法: 采用HPLC梯度洗脱法,对怀地黄药材进行测定,色谱条件,Dikma DiamonsilC₁₈柱（4.6 mm×250 mm,5 μm）,乙腈-0.1%磷酸水系统梯度洗脱,流速1.0 mL·min^-1,柱温35℃,检测波长205 nm。 结果: 标定了13个共有峰,建立了HPLC指纹图谱,该图谱的相关系数均在0.99以上。 结论: 该方法精确可靠,重复性好,可为控制怀地黄药材内在质量提供科学依据。     

华南忍冬藤HPLC指纹图谱研究

目的: 建立华南忍冬藤的HPLC指纹图谱,为华南忍冬藤质量评价提供理论依据。 方法: 采用RP-HPLC。Kromasil C₁₈色谱柱(4.6 mm×250 mm,5 μm),乙腈-0.3%甲酸溶液梯度洗脱,流速1.0 mL·min^-1,柱温30 ℃,检测波长238 nm。图谱用国家药典委员会"相似度评价软件(2004A)"处理分析。 结果: 确立了12个共有峰的共有模式,10批红腺忍冬藤样品指纹图谱相似度高。 结论: 方法简便、准确、重复性好,为评价华南忍冬藤药材的质量提供了依据。     

茜草药材的HPLC指纹图谱研究

目的: 建立河南不同产地茜草药材的 HPLC指纹图谱分析方法,为茜草药材的质量评价提供参考依据。 方法: 采用高效液相色谱法,Phenomenex ODS C₁₈柱（4.60 mm×250 mm,4 μm）,甲醇-0.2%磷酸水梯度洗脱,检测波长275 nm,流速0.8 mL·min^-1,柱温25℃。 结果: 以大叶茜草素为参照峰,确定11个共有峰,测定了14批茜草药材HPLC指纹图谱与对照图谱的相似度。 结论: 所建立的方法可用于茜草药材指纹图谱测定,并可对其今后规范药用资源及质量评价提供科学依据。     

多波长融合玄参饮片HPLC指纹图谱研究

目的：建立玄参多波长融合HPLC指纹图谱,为玄参的质量提供新方法。方法：采用多波长融合HPLC测定不同产地玄参饮片。色谱条件：Thermo C₁₈柱,乙腈-0.1%甲酸溶液为流动相梯度洗脱。多波长检测（0~5 min为230 nm,5~13 min为210 nm,13~23 min为320 nm,23~50 min为278 nm,流速1 mL·min^-1）,柱温30 ℃。结果：建立了多波长融合玄参饮片HPLC指纹图谱共有模式,确认25个共有峰,标定其中6个峰,10批玄参样品相似度均>0.90。结论：建立的指纹图谱色谱峰数较多,色谱信息量大,适用于玄参饮片的质量评价。     

鹿茸草药材的HPLC指纹图谱

目的：建立鹿茸草的HPLC指纹图谱,为鹿茸草的质量控制提供依据。方法：采用HPLC-UV分析鹿茸草的指纹图谱,色谱条件为Agilent HC C₁₈色谱柱（4.6 mm×250 mm,5 μm）,以乙腈（A）-0.4%磷酸水（B）梯度洗脱,流速 0.8 mL·min^-1,柱温35 ℃,检测波长254 nm,进样量10 μL。利用中药色谱指纹图谱相似度评价系统（2004A 版）对不同批次鹿茸草的HPLC图进行比较分析。结果：建立了鹿茸草的 HPLC 指纹图谱,以木犀草素为参照峰,确立了鹿茸草指纹图谱中的13个共有峰。结论：所建立的指纹图谱稳定性和重复性均好,可用于鹿茸草药材的常规质量控制。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.     

瑞香科五属植物中酚性聚合物的结构特征、分布及药理活性研究进展

瑞香科植物广泛分布于世界热带和温带地区,其中多种植物可供药用。在对该科植物的研究中发现,黄酮类、木脂素和香豆素等酚性化合物的低聚体为其重要的特征性成分,药理活性主要表现在抗肿瘤、抗炎、抗氧化、抗菌和抗病毒等方面。对已发现的酚性聚合体的化学结构、植物来源及药理活性进行综述,为瑞香科植物今后的研究和开发利用提供参考。     

HPLC法同时测定沉香化滞丸中11种成分

目的采用HPLC梯度洗脱法同时测定沉香化滞丸中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚11种成分。方法采用Thermo Syncronis C18色谱柱(250 mm×4.6 mm,5μm),流动相为水-乙腈,梯度洗脱:0～10 min,20%乙腈;10～20 min,20%～40%乙腈;20～24 min,40%乙腈;24～26 min,40%～52%乙腈;26～30 min,52%乙腈;30～31 min,52%～90%乙腈;31～35 min,90%乙腈;35～40 min,90%～100%乙腈;40～43min,100%乙腈;43～45min,100%～20%乙腈;检测波长215nm,体积流量1.0m L/min,柱温30℃,进样量20μL。结果各成分在43 min内分离良好,沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚的线性范围分别为1.4～13.6、10.0～200.0、31.5～315.0、1.0～120.1、1.8～50.6、0.93～10.1、1.8～30.0、0.2～40.3、1.8～18.1、1.7～25.0、0.45～10.70μg/mL;样品中各成分的平均回收率均在98.90%～100.87%;11种成分精密度RSD在0.55%～1.54%;供试品溶液在30 h内稳定性良好,RSD在0.75%～1.94%;重复性RSD在0.39%～1.73%。6批次样品中沉香四醇、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、大黄素、厚朴酚、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚质量分数分别为92.0～201.0、511.5～9 033.0、5 475.0～12 635.5、54.5～5 095.5、192.0～2 137.5、117.0～391.5、106.5～1 281.5、13.0～136.5、93.5～199.0、177.0～1 207.0、33.5～251.5μg/g。结论本方法准确、快速、简便,重复性好,精密度高,适用于沉香化滞丸中多种活性成分的定量分析。