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1.
缪海均  佘佳红  刘皋林 《中成药》2002,24(12):934-935
目的:测定藿香正气软胶囊(藿香油,紫苏油,陈皮,厚朴等)中厚朴酚、和厚朴酚的含量。方法:样品经超临界流体萃取后,用毛细管气相色谱测定含量。结果:厚朴酚的平均回收率为96.81%(RSD=2.81%,n=5);和厚朴酚为97.75%(RSD=2.36%,n=5)。结论:方法简便、快速、准确。  相似文献   

2.
快速提取及测定紫油厚朴中厚朴酚及和厚朴酚的研究   总被引:1,自引:0,他引:1  
目的研究厚朴中厚朴酚及和厚朴酚的快速提取及测定。方法采用正交实验用ASE300快速溶剂萃取机进行提取方法的研究,用HPLC法厚朴酚及和厚朴酚的含量测定,流动相为甲醇-水-冰醋酸(85∶15∶0.25),流速为1.0 m l/m in,检测波长为294 nm,柱温为23℃。结果研究表明,用60%的乙醇,71℃的提取温度在快速萃取系统提取3次(10m in/次),厚朴中的厚朴酚、和厚朴酚以及总酚的提取率分别为94.59%,85.45%和88.81%;RP-HPLC方法较好,结果理想。结论该研究得到了很好的快速提取及测定厚朴中厚朴酚及和厚朴酚的方法。  相似文献   

3.
微波辅助提取厚朴中厚朴酚及和厚朴酚的研究   总被引:2,自引:0,他引:2  
姜宁  刘晓鹏 《中华中医药杂志》2008,23(11):1012-1015
目的:进行微波辅助提取厚朴中厚朴酚及和厚朴酚的研究.方法:采用正交实验用微波辅助进行提取方法的研究;用HPLC法测定厚朴酚及和厚朴酚含量,流动相:甲醇-水-冰醋酸(85∶15∶0.25),流速为1.0 ml/min,检测波长为294nm,柱温为23℃.结果:用中等强度的微波功率,酒精度75%,提取时间为4min,料液比1∶40的提取条件可很好地提取厚朴药材中的厚朴酚及和厚朴酚,和厚朴酚、厚朴酚以及总酚的提取率分别为92.33%、73.59%和83.32%;RP-HPLC方法较好,结果理想.结论:本研究得到了微波辅助提取厚朴中厚朴酚及和厚朴酚的方法.  相似文献   

4.
高辉  王雪萍 《中医药学刊》2008,26(2):425-426
建立了厚朴温中丸中厚朴酚与和厚朴酚的含量测定方法。方法:采用HPLC测定厚朴酚与和厚朴酚的含量。结果:厚朴酚与厚朴酚含量测定方法的线性范围分别为0.488-2.440μg(Ch i-Square:27.44)和0.362-1.810μg(Ch i-Square:36.07),平均回收率分别为97.0%,RSD=1.3%(n=5)和96.8%,RSD=1.4%(n=5)。结论:所建立的方法准确可行,重复性好,可作为厚朴温中丸中控制厚朴含量的质量标准。  相似文献   

5.
目的:建立HPLC法同时测定复方藿香丸中和厚朴酚与厚朴酚的含量。方法:采用Agilent 5 TC-C18色谱柱(4.6mm×250mm,5μm),以甲醇-乙腈-0.3%磷酸(44∶19∶37)为流动相,流速为1.0mL/min,检测波长294nm,检测柱温30℃。结果:和厚朴酚、厚朴酚的进样浓度分别在13.00~208.00g/L(r=0.99995)和26.23~419.68 g/L(r=0.99996)呈良好线性关系;和厚朴酚、厚朴酚的平均回收率(n=6)分别为98.58%(RSD%=2.29)、101.29%(RSD%=1.75);5个批次复方藿香丸样品中和厚朴酚、厚朴酚的含量测定结果分别为3.41~12.26mg/袋、8.59~14.42mg/袋。结论:建立的HPLC法可用于复方藿香丸中和厚朴酚与厚朴酚的含量测定。  相似文献   

6.
超声法提取厚朴中厚朴酚及和厚朴酚的研究   总被引:5,自引:0,他引:5  
本文进行了超声法提取厚朴中厚朴酚及和厚朴酚的研究,研究结果表明:用超声强度中等、酒精度100%、提取时间为24 h以及料液比1∶40的提取条件可很好地提取厚朴药材中的厚朴酚及和厚朴酚。厚朴酚、和厚朴酚以及总酚的提取率分别为95.45%、79.46%和89.57%。RP-HPLC方法较好,结果理想。  相似文献   

7.
<正> 厚朴为一常用中药,其主要成分为厚朴酚、和厚朴酚及挥发油等。关于和厚朴酚、厚朴酚含量测定有高效液相层析法,气相层析法,薄层层析紫外分光光度法。我们用和厚朴酚作为对照品,直接测定药材中厚朴酚及和  相似文献   

8.
建立了厚朴温中丸中厚朴酚与和厚朴酚的含量测定方法。方法:采用HPLC测定厚朴酚与和厚朴酚的含量。结果:厚朴酚与厚朴酚含量测定方法的线性范围分别为0.488~2.440μg(Ch i-Square:27.44)和0.362~1.810μg(Ch i-Square:36.07),平均回收率分别为97.0%,RSD=1.3%(n=5)和96.8%,RSD=1.4%(n=5)。结论:所建立的方法准确可行,重复性好,可作为厚朴温中丸中控制厚朴含量的质量标准。  相似文献   

9.
目的:(建立用高效液相色谱法同时测定厚朴温中泡腾颗粒剂中厚朴酚、和厚朴酚含量的方法.方法:采用依利特C18(4.6mm×200mm,5μm)色谱柱,以甲醇-乙腈-水(31.5:30.0:38.5)为流动相,检测波长为294nm.结果:厚朴酚在0.27~4.08μg有良好的线性关系,r=0.999 7,平均加样回收率为98.50%,RSD为1.85%;和厚朴酚在0.06~0.84μg有良好的线性关系,r=0.999 7,平均加样回收率为98.64%,RSD为1.91%.结论:本方法快速简便,可用于厚朴温中泡腾颗粒剂中厚朴酚、和厚朴酚含量测定.  相似文献   

10.
目的比较按国标和广东地标生产的姜制厚朴中厚朴酚与和厚朴酚的含量差异。方法采用HPLC测定不同来源姜制厚朴中厚朴酚及和厚朴酚的含量。结果厚朴酚质量浓度在1.38~69.00μg.mL-1与峰面积有良好线性关系,r=0.9996,平均回收率为100.66%,RSD=1.15%(n=5);和厚朴酚质量浓度在0.49~24.40μg.mL-1与峰面积有良好线性关系,r=0.9998,平均回收率为100.04%,RSD=1.05%(n=5)。结论国标和广东地标姜制厚朴中厚朴酚与和厚朴酚总含量总体差别不大,各地姜制厚朴质量差别大。  相似文献   

11.
目的探讨药对苍术-厚朴的配伍对苍术素、厚朴酚、和厚朴酚溶出率的影响。方法采用水煎煮法、乙醇回流提取法分别提取药对苍术-厚朴及其单味药中的有效成分,然后采用高效液相色谱法对苍术素、厚朴酚、和厚朴酚的含量进行分析。结果苍术-厚朴药对水提液,苍术-厚朴药对醇提液中苍术素、厚朴酚、和厚朴酚含量明显高于单味药材,其中苍术-厚朴药对醇提液中含量最高,分别为1.8486、1.0876、0.4840 mg·g-1。结论苍术-厚朴药对在体外配伍后更能促进有效成分的溶出。  相似文献   

12.
厚朴及其炮制品中厚朴酚与和厚朴酚含量测定   总被引:9,自引:1,他引:9       下载免费PDF全文
曾诠  宋学华  张泉  屠万蒨  张琳 《中草药》1996,27(1):11-13
采用薄层扫描法测定生品、炒黄、炒焦、姜炙厚朴及市售商品厚朴、姜炙厚朴中厚朴酚、和厚朴酚的含量,结果表明,厚朴炮制后,该2成分含量均有下降,炒焦降低3O%以上,炒黄、姜炙降低约20%。市售商品厚朴含量差异较大。  相似文献   

13.
The antimicrobial activity of honokiol and magnolol, the main constituents of Magnolia officinalis was investigated. The antimicrobial activity was assayed by the agar dilution method using brain heart infusion medium and the minimum inhibitory concentration (MIC) were determined for each compound using a twofold serial dilution assay. The results showed that honokiol and magnolol have a marked antimicrobial effect (MIC = 25 microg/mL) against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Micrococcus luteus and Bacillus subtilis, but did not show antimicrobial activity (MIC > or = 100 microg/mL) for Shigella flexneii, Staphylococcus epidermidis, Enterobacter aerogenes, Proteus vulgaris, Escherichia coli and Pseudomonas aeruginosa. Our results indicate that honokiol and magnolol, although less potent than tetracycline, show a significant antimicrobial activity for periodontal pathogens. Hence we suggest that honokiol and magnolol might have the potential to be an adjunct in the treatment of periodontitis.  相似文献   

14.
In our previous study using an improved elevated plus-maze in mice, the oriental herbal medicine Saiboku-to prolonged the time spent in open arms, showing an anxiolytic effect, and the effect was mainly caused by honokiol derived from magnolia. This study was carried out to compare the anxiolytic potentials of honokiol and water extracts of three magnolia samples; two being Kara-koboku (Magnolia officinalis) (KA: from Zhejiang-sheng, China; honokiol 0.25% and magnolol 1.16%, and KB: from Sichuan-sheng, China; honokiol 1.72% and magnolol 1.71%), and one being Wa-koboku (Magnolia obovata) (WA: from Iwate-ken, Japan; honokiol 0.32% and magnolol 0.81%). Seven daily treatments with 0.1-1 mg/kg honokiol, but not 0.2 and 1 mg/kg magnolol, revealed an anxiolytic effect with the peak potential at 0. 2 mg/kg. The anxiolytic potentials of 40 and 80 mg/kg KA, which contained the highest amount of magnolol, were almost equivalent to those of 0.1 and 0.2 mg/kg honokiol, respectively. KB, at 11.6 mg/kg, and 62.5 mg/kg WA resulted in almost the same anxiolytic potential as that of 0.2 mg/kg honokiol. No significant change in the ambulatory activity was produced by any drug treatment. These results suggest that honokiol is the chemical responsible for the anxiolytic effect of the water extract of magnolia and that the other chemicals including magnolol in magnolia scarcely influence the effect of honokiol. It is also considered that the elevated plus-maze test is applicable for evaluation of the content of honokiol in magnolia.  相似文献   

15.
厚朴的炮制研究   总被引:1,自引:1,他引:0  
目的:明确厚朴的炮制沿革,对已有的相关研究进行综述。方法:查阅国内外相关文献并进行归纳总结。结果:厚朴炮制方法始载于《雷公炮炙论》,历代本草均有其炮制方法的记载,多为姜制,炮制目的为"不以姜制,则棘人舌喉"。现今厚朴沿用的炮制方法是净制去粗皮、切丝以姜汁制。姜厚朴的质量多以厚朴酚与和厚朴酚的含量来评价。结论:姜厚朴的炮制工艺不统一,质量标准有待提高。  相似文献   

16.
超声强化超临界提取厚朴酚与和厚朴酚的工艺研究   总被引:4,自引:0,他引:4  
目的探究超声对超临界提取的强化作用。方法在超临界提取厚朴稳定的工艺参数下,以提取物中厚朴酚与和厚朴酚的量和提取率为指标,比较了超声强化超临界提取、超声提取、超临界提取的效果。结果超声强化超临界的提取效果明显优于两者的单独提取。结论超声可强化超临界对厚朴有效成分的提取。  相似文献   

17.
超临界流体提取中药中的厚朴酚   总被引:16,自引:1,他引:15  
刘本 《中成药》1999,21(7):331-333
用超临界流体提取厚朴,藿香正气胶囊和藿香正气丸中的厚朴酚,高效液相色谱用于分析提取物。结果表明,10%甲醇调节的超临界二氧化碳能有效地提取中药中的厚朴酚。该分析方法具简便,快速,准确的优点。  相似文献   

18.
Honokiol and magnolol, phenolic compounds isolated from the stem bark of Magnolia officinalis, have been demonstrated to increase choline acetyltransferase activity, inhibit acetylcholinesterase, promote potassium-induced acetylcholine release and exhibit neurotrophic function in in vitro studies. The objective of the present study was to determine the effect of these compounds on hippocampal acetylcholine release in conscious, freely-moving rats. 10(-4) M-10(-6) M of honokiol or magnolol was perfused into rat hippocampus via a dialysis probe. The results showed that at 10(-4) M concentration, honokiol and magnolol markedly increased extracellular acetylcholine release to 165.5+/-5.78% and 237.83+/-9.47% of the basal level, respectively. However, lower concentrations of either compounds failed to elicit significant acetylcholine release. This result suggests that a high dose of honokiol or magnolol may enhance in vivo hippocampal acetylcholine release.  相似文献   

19.
湖北恩施产厚朴苗干枝皮酚类成分研究   总被引:3,自引:0,他引:3  
目的研究厚朴2年生实生苗干枝皮的厚朴酚与和厚朴酚含量,及其甲醇溶性成分HPLC图谱特征,为厚朴药材的良种选育提供依据.方法采用高效液相色谱法测定厚朴酚、和厚朴酚含量;比较10批恩施产厚朴2年生实生苗干枝皮样品甲醇溶性成分的HPLC图谱相似度.结果恩施厚朴2年生实生苗干枝皮的厚朴酚与和厚朴酚含量较高,并建立了HPLC指纹图谱.结论本研究可为厚朴药材的良种选育与苗期鉴别提供参考.  相似文献   

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