赞育丹对不育大鼠性腺功能调节作用的实验研究

实验[目的]研究赞育丹对腺嘌呤所诱发的睾丸生精障碍不育大鼠的影响。[方法]以腺嘌呤灌胃Wistar大鼠,诱发睾丸细胞生精障碍,观察赞育丹制剂给药后实验动物精子计数、睾丸形态学及性激素(T、FSH、LH)的改变。[结果]赞育丹可增加睾丸生精障碍性不育大鼠精子数量;增加睾丸附睾重量。提高前列腺和精囊指数,提高性激素水平;使受损的睾丸组织明显改善。[结论]赞育丹对腺嘌呤诱发的大鼠睾丸生精障碍不育有治疗作用。     

帕罗西汀联合奥氮平治疗抑郁症并发睡眠障碍的效果分析

〔摘 要〕 目的：观察分析帕罗西汀联合奥氮平治疗抑郁症并发睡眠障碍的临床疗效。方法：选取洛阳市第五人民医院2019 年 2 月至 2020 年 2 月收治的 64 例抑郁症并发睡眠障碍患者为研究对象,在患者自愿参与本研究的前提下通过随机法分为对照组（32 例：帕罗西汀治疗）与观察组（32 例：帕罗西汀联合奥氮平治疗）,比较两组患者治疗效果。结果：观察组治疗后 5– 羟色胺水平明显高于对照组,差异具有统计学意义（P ＜ 0.05）。观察组患者治疗后总有效率（96.88 %）比对照组（78.13 %）高,且观察组患者治疗 3 个疗程后睡眠潜伏期、睡眠总时长、觉醒次数等睡眠观察指标均优于对照组,差异均具有统计学意义（P ＜ 0.05）。观察组患者不良反应发生率（18.75 %）比对照组（9.38 %）略高,但组间比较,差异无统计学意义（P ＞ 0.05）。结论：抑郁症并发睡眠障碍患者帕罗西汀联合奥氮平治疗效果明显优于单一帕罗西汀治疗。     

帕罗西汀联合曲唑酮治疗抑郁症合并性功能障碍的疗效

目的：探究帕罗西汀联合曲唑酮治疗抑郁症合并性功能障碍的疗效。方法：选择福建医科大学附属协和医院2022年1月至2023年1月诊治的80例抑郁症合并性功能障碍男性患者,依据不同治疗的方式分为观察组（帕罗西汀与曲唑酮联合治疗的方式）与对照组（单用帕罗西汀治疗）,各40例。比较两组患者焦虑抑郁评分、国际勃起功能指数–5量表（IIEF–5）评分、疾病疗效、不良反应。结果：观察组患者治疗后焦虑自评量表（SAS）、抑郁自评量表（SDS）评分均低于对照组,差异具有统计学意义（P <0.05）;治疗后,观察组患者IIEF–5评分高于对照组,差异具有统计学意义（P <0.05）;观察组患者总有效率为95.00%,高于对照组的70.00%,差异具有统计学意义（P <0.05）;观察组患者不良反应发生率为2.50%,低于对照组的22.50%,差异具有统计学意义（P <0.05）。结论：对抑郁症合并性功能障碍患者予以帕罗西汀与曲唑酮联合治疗,将明显改善患者焦虑抑郁评分,提升IIEF–5评分,降低不良反应的发生率,提升对抑郁症合并性功能障碍的治愈率。     

育麟方治疗女性性功能障碍初探

中医学认为女性性功能障碍多因肾阳虚衰、肝气郁结所致,温肾养肝是治疗的基本大法。育麟方重在温补肾阳,并辅以柔养精血,故将此方用于治疗女性性功能障碍,在现代药理学探究亦有据可循。     

帕罗西汀联合认知行为心理治疗对产后抑郁症的疗效

〔摘 要〕 目的：探讨帕罗西汀联合认知行为心理治疗对产后抑郁症的临床疗效。方法：选取 2018 年 1 月至 2019 年 12 月 东莞市东华医院收治的产后抑郁症患者 90 例,随机分为对照组和观察组,每组 45 例。对照组给予盐酸帕罗西汀肠溶缓 释片治疗,观察组给予盐酸帕罗西汀肠溶缓释片联合认知行为心理治疗,比较两组患者爱丁堡产后抑郁量表（EPDS）评 分、副反应量表（TESS）评分和治疗有效率。结果：治疗前两组患者 EPDS 评分、TESS 评分比较,差异无统计学意义 （P ＞ 0.05）,治疗后观察组 EPDS 评分、TESS 评分均低于对照组,差异具有统计学意义（P ＜ 0.05）;治疗后观察组治 疗总有效率为 93.33 %,高于对照组的 77.78 %,差异具有统计学意义（P ＜ 0.05）。结论：产后抑郁症在帕罗西汀药物治 疗的同时配合认知行为心理治疗,可更好地控制抑郁状态,提升治疗总有效率。     

盐酸帕罗西汀联合天丹通络胶囊治疗脑卒中后抑郁症的疗效观察

目的 探讨盐酸帕罗西汀联合天丹通络胶囊治疗脑卒中后抑郁症（PSD）的临床疗效。方法 将纳入PSD组82例患者随机分为治疗组42例和对照组40例。在常规治疗的基础上,对照组给予盐酸帕罗西汀片口服,20 mg/次,1次/d,早餐后服用。治疗组在对照组的基础上联合使用天丹通络胶囊,2.0 g/次,3次/d。两组疗程均为6周。治疗前后以汉密尔顿抑郁量表（HAMD）评定抑郁程度,以中国卒中量表（CSS）和日常生活能力量表（ADL）分别评定神经功能缺损程度和日常生活能力。结果 治疗2、4、6周后,两组的HAMD量表值均低于治疗前,差异有统计学意义（P〈0.05）;治疗组的的总有效率高于对照组,差异有统计学意义（P〈0.05）。治疗6周后,治疗组的CSS评分较治疗前显著下降,ADL评分较治疗前显著上升,但治疗组ADL评分明显高于对照组,两组比较差异有统计学意义（P〈0.05）。结论 盐酸帕罗西汀联合天丹通络胶囊治疗PSD比单纯使用盐酸帕罗西汀起效快,且能促进神经功能恢复,是值得临床推广的一种治疗方法。     

基于网络药理学和分子对接探讨六君子汤治疗胃肠功能障碍的作用机制※

目的 基于网络药理学及分子对接技术分析六君子汤治疗胃肠功能障碍的核心成分、共作用靶点及通路。方法 通过中药系统药理学数据库和分析平台（TCM-SP）数据库进行检索并筛选六君子汤的活性成分及作用靶点,运用Disgenet、MOIM、Genecards数据库检索胃肠功能障碍的相关作用靶点,取二者靶点交集,应用STRING平台及Cytoscape 3.10.0软件构建六君子汤-成分-靶点蛋白-疾病互作网络图;凭借微生信平台对所得交集靶点进行富集分析并绘制GO和KEGG富集分析图,最终使用pymol和AutoDock软件实现分子对接可视化。结果 六君子汤药物活性成分共131个,药物靶点218个,疾病相关靶点1376个,药物与疾病共靶点108个,GO分析中生物过程3584条,细胞组成186条,分子功能348条,KEGG分析获得通路229条,分子对接结果显示主要活性成分与核心靶点对接成功,具有良好的结合活性。结论 六君子汤可能通过槲皮素、7-甲氧基-2-甲基异黄酮、木犀草素、豆甾醇、山奈酚等有效成分,作用于STAT3、TP53、MAPK3、AKT1、MAPK1等靶点,参与糖尿病并发症通路、卡波西肉瘤相关病毒感染等信号通路,实现调节胃肠动力、抗炎等作用,从而改善胃肠功能障碍患者预后。     

小剂量西地那非和帕罗西汀联合用药治疗 男性性功能障碍的疗效观察

〔摘 要〕 目的：分析对性功能障碍患者治疗中,联合使用小剂量西地那非与帕罗西汀的效果。方法：选取兴宁市人民 医院 2019 年 3 月至 2020 年 8 月期间收治的 84 例男性性功能障碍患者,随机分成对照组与观察组,各 42 例。对照组患者 所采用的治疗方式为小剂量西地那非,观察组患者在对照组的基础上加用小剂量帕罗西汀治疗,观察两组患者国际勃起功 能评分表（IIEF–5）评分、不良反应、情绪状态。结果：治疗前,两组患者的 IIEF–5 评分比较,差异无统计学意义（P ＞ 0.05）。 治疗后,观察组患者 IIEF–5 评分高于对照组,差异具有统计学意义（P ＜ 0.05）;两组患者不良反应发生率比较,差异无统 计学意义（P ＞ 0.05）;治疗前,两组患者的抑郁自评量表（SDS）、焦虑自评量表（SAS）评分比较,差异无统计学意义 （P ＞ 0.05）。治疗后,观察组患者的 SDS、SAS 评分低于对照组,差异具有统计学意义（P ＜ 0.05）。结论：对性功能 障碍治疗中,采用小剂量西地那非联合帕罗西汀,可帮助患者改善勃起状况,缓解不良情绪,且安全性较高。     

新鲜洋葱汁对帕罗西汀所致雄性大鼠性功能障碍的影响及其机制研究

目的探讨新鲜洋葱汁对正常雄性大鼠和帕罗西汀所致性功能障碍雄性大鼠性功能和血清睾酮水平的影响,为后期临床研究提供实验依据。方法将有性行为能力的70只雄性大鼠随机分为7组,每组10只。对照组给予蒸馏水灌胃,0.5 m L洋葱汁组、1.0 m L洋葱汁组血清睾酮水平2.0 m L洋葱汁组分别给予相应剂量的新鲜洋葱汁灌胃,帕罗西汀组给予帕罗西汀悬浮液10 mg/kg灌胃,帕罗西汀加1.0 m L洋葱汁组、帕罗西汀加2.0 m L洋葱汁组分别给予帕罗西汀悬浮液10 mg/kg和相应剂量的新鲜洋葱汁灌胃,均1次/d,连续灌胃20 d。在末次灌胃结束后3 h,记录各组大鼠性行为参数及血清睾酮水平。结果 1.0 m L洋葱汁组和2.0 m L洋葱汁组大鼠的骑跨潜伏期、骑跨次数均明显低于对照组和0.5 m L洋葱汁组(P均0.05),交配有效率和血清睾酮水平均明显高于对照组和0.5 m L洋葱汁组(P均0.05);1.0 m L洋葱汁组和2.0 m L洋葱汁组大鼠的插入潜伏期和1.0 m L洋葱汁组大鼠的射精潜伏期均明显低于对照组(P均0.05);1.0m L洋葱汁组大鼠的插入潜伏期和射精潜伏期均明显低于0.5m L洋葱汁组(P均0.05)。帕罗西汀组大鼠的射精潜伏期明显长于对照组(P0.05),血清睾酮水平明显低于对照组(P0.05);帕罗西汀加1.0 m L洋葱汁组、帕罗西汀加2.0 m L洋葱汁组大鼠的骑跨潜伏期和帕罗西汀加2.0 m L洋葱汁组大鼠的骑跨次数均明显少于对照组和帕罗西汀组(P均0.05);帕罗西汀加1.0 m L洋葱汁组、帕罗西汀加2.0 m L洋葱汁组大鼠的射精潜伏期均明显低于帕罗西汀组(P均0.05),血清睾酮水平均明显高于帕罗西汀组(P均0.05)。结论新鲜洋葱汁可以明显增强雄性大鼠的性功能,并可以通过提高血清睾酮水平来缓解帕罗西汀引起的性功能障碍,增强性交配行为。     

帕罗西汀联合九味镇心颗粒治疗心脾两虚抑郁症的效果

〔摘 要〕 目的：分析对心脾两虚抑郁症患者临床用药中使用帕罗西汀联合九味镇心颗粒的治疗效果。 方法：选取北京市朝阳区第三医院 2020 年 8 月至 2022 年 8 月收治的 72 例心脾两虚抑郁症患者,采用随机数字表法分为对照组和观察组,各 36 例。对照组单一服用帕罗西汀治疗,观察组使用帕罗西汀联合九味镇心颗粒治疗,比较两组患者治疗前后的匹茨堡睡眠质量指数量表（PSQI）评分、贝克抑郁量表（BDI）评分、生活质量及用药不良反应。 结果：治疗后,两组患者 PSQI评分均降低,且观察组 PSQI 评分低于对照组,差异具有统计学意义（P ＜ 0.05）;治疗后,两组患者 BDI 评分均降低,且观察组 BDI 评分均低于对照组,差异具有统计学意义（P ＜ 0.05）;治疗后,观察组患者生活质量评分高于对照组,不良反应发生率低于对照组,差异具有统计学意义（P ＜ 0.05）。 结论：心脾两虚抑郁症患者采用帕罗西汀联合九味镇心颗粒进行治疗,提升患者睡眠质量和生活质量,且联合用药并不增加治疗过程中的不良反应。     

Cochlospermum vitifolium induces vasorelaxant and antihypertensive effects mainly by activation of NO/cGMP signaling pathway

Aim of the study

Cochlospermum vitifolium is a medicinal plant used for the treatment of diabetes, hepatobilary and cardiovascular illnesses. The aim of current study was to determine the in vivo antihypertensive and in vitro functional vasorelaxant mechanism of methanol extract of Cochlospermum vitifolium (MECv) and naringenin (NG).

Materials and methods

Test material was assayed on rat isolated aorta rings test with- and without-endothelium to determine their vasorelaxant mechanism. Also, the in vivo antihypertensive effect was evaluated on spontaneously hypertensive rat (SHR) model. In addition, presence of NG into the extract was confirmed by reverse phase high performance liquid chromatography (RP-HPLC) analysis.

Results

MECv (120 mg/kg) and NG (50 and 160 mg/kg) showed acute antihypertensive effects on SHR when systolic and diastolic pressure were decreased at 1 h and 24 h after administration, respectively. Vasorelaxant effect of MECv and NG was shifted to the right when endothelium-intact aortic rings were pre-incubated with L-NAME (10 μM) and ODQ (1 μM). Also, NG relaxant curves were displaced to the right in the presence of tetraethylammonium (TEA, 1 mM) and 2-aminopyridine (2-AP, 100 μM) on endothelium-denuded aortic rings.

Conclusion

Experiments described above showed that MECv play an important role in hypertension regulation through NO synthesis and may be PGI₂ production and potassium channel activation on excessive endothelial dysfunction conditions. Unfortunately, presence of NG into the extract is not significant on bioactivity of the extract; however, this compound could be tested and evaluated as structural scaffold for future drug design for development of antihypertensive agents.     

电针对坐骨神经分支选择性损伤大鼠脊髓L-Arg/NO/cGMP通路的影响

目的 :观察坐骨神经分支选择性损伤(SNI)大鼠脊髓相应节段左旋精氨酸(L-Arg)转运体2(CAT-2)、一氧化氮合酶(NOS)与一氧化氮(NO)和环鸟苷酸(cGMP)的变化及电针对其的影响,探讨电针干预神经病理性痛的脊髓机制。方法:SD大鼠随机分为假手术组、模型组、电针组和NOS抑制剂(LNAME)组,每组30只。采用SNI法制备神经病理性痛模型,假手术组仅分离不结扎。电针组电针大鼠损伤侧"委中""环跳"穴,频率2 Hz,波宽0. 6 ms,起始电流强度1 mA,每10 min递增1 mA,刺激时间30 min,1次/d,连续7 d;L-NAME组予L-NAME 60 mg·kg~(-1)·d~(-1)腹腔注射,连续7 d。造模前及SNI后10、16 d分别测定机械痛阈。RT-PCR法测定脊髓CAT-2 mRNA与诱导型NOS(iNOS)mRNA的表达,Western blot法测定脊髓iNOS的表达,硝酸/亚硝酸还原酶法与放射免疫分析法分别测定NO(NO_2~-/NO_3~-)与cGMP的含量。结果:与假手术组比较,SNI后10 d其他各组机械痛阈值均降低(P<0. 01);与模型组比较,电针组和LNAME组SNI后16 d时机械痛阈值均升高(P<0. 01,P<0. 05)。与假手术组比较,模型组脊髓CAT-2mRNA、iNOS mRNA、iNOS蛋白表达及NO_2~-/NO_3~-与cGMP含量均升高(P<0. 01,P<0. 05);与模型组比较,电针组和L-NAME组脊髓CAT-2 mRNA、iNOS mRNA、iNOS蛋白表达及NO_2~-/NO_3~-与cGMP含量均下降(P<0. 05,P<0. 01),且L-NAME组CAT-2 mRNA、iNOS mRNA、iNOS蛋白表达比电针组降低更显著(P<0. 05)。结论:电针缓解疼痛的作用机制之一,可能是通过有效下调神经病理性痛状态下脊髓L-Arg/NO/cGMP通路的功能而实现的。     

Turnera diffusa Wild (Turneraceae) recovers sexual behavior in sexually exhausted males

Ethnopharmacological relevance

In folk medicine, Turnera diffusa Wild (Turnera diffusa, Turneraceae) is considered as an aphrodisiac, but its ability to restore copulation in sexually inhibited subjects has not been reported.

Aim of the study

To determine whether Turnera diffusa recovers sexual behavior in sexually exhausted (SExh) male rats and to identify the main components in an aqueous extract.

Materials and methods

SExh males were treated with Turnera diffusa, 20–80 mg/kg, yohimbine, 2 mg/kg, or vehicle.

Results

Yohimbine and Turnera diffusa (80 mg/kg) significantly increased the percentage of males achieving one ejaculatory series and resuming a second one. In addition, Turnera diffusa significantly reduced the post-ejaculatory interval. These effects were not associated to an increase in locomotor activity or anxiety-like behaviors. The HPLC–ESI-MS analysis showed the presence of caffeine, arbutine, and flavonoids as the main compounds in the active extract.

Conclusion

The results support the use of Turnera diffusa as an aphrodisiac in traditional medicine and suggest possible therapeutic properties of Turnera diffusa on sexual dysfunction. The flavonoids present in active extract may participate in its pro-sexual effect, which is analogous to those produced by yohimbine, suggesting a shared mechanism of action.     

黄体功能不全性不孕的中医辨证及护理干预的效果研究

目的：探讨中医辨证联合护理干预治疗黄体功能不全性不孕的应用效果;方法：选择黄体功能不全性不孕患者76例,随机分为治疗组与对照组38例。对照组给予黄体酮胶囊,治疗组给予中医辨证治疗,两组均给予护理干预措施,比较治疗前后基础体温（BBT）、孕酮（P）、及治疗效果;结果：治疗组治愈率（57．89％）、总有效率（89．74％）、高温相持续时间（13．21±1．62）d、高低温度差（0．39±0．04）℃、血清P（12．05±2．14）ng／M1均明显高于对照组（P〈0．05）。结论：中医辨证治疗及护理干预可有效改善患者临床症状,提高治疗水平。     

芍药苷和芍药内酯苷的抗抑郁作用与NO/cGMP信号转导通路的相关性

目的:比较赤芍、白芍及其有效成分芍药苷、芍药内酯苷对小鼠的抗抑郁作用及其与NO/c GMP信号转导通路的相关性。方法:选取小鼠悬尾实验模型,给予赤芍水提物、白芍水提物,记录小鼠悬尾不动时间。采用相同实验方法给予小鼠芍药苷和芍药内酯苷,并检测小鼠大脑皮质及海马组织中NO/c GMP通路相关指标。结果:与模型组比较,2 g/kg赤芍和2 g/kg、1 g/kg白芍组小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与2 g/kg赤芍同剂量组比较,白芍的小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组、20 mg/kg芍药内酯苷组的小鼠悬尾不动时间及NO、c GMP含量明显减少,差异有统计学意义(P0.05);且与10 mg/kg芍药苷同剂量组比较,芍药内酯苷组的小鼠悬尾不动时间及NO含量明显减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组及20mg/kg芍药内酯苷组的小鼠n NOS、GluR1mRNA表达明显减少,差异有统计学意义(P0.05)。结论:白芍具有抗抑郁作用,芍药苷和芍药内酯苷是白芍抗抑郁作用的物质基础;与白芍比较,赤芍的抗抑郁作用较弱,与其主要含有抗抑郁作用较弱的芍药苷,而不含抗抑郁作用较强的芍药内酯苷有关。     

Effect of lipid-based suspension of Epimedium koreanum Nakai extract on sexual behavior in rats

Ethnopharmacological relevance

Herba of Epimedium koreanum is used in traditional Chinese and Korean herbal medicine as a potent enhancer of erectile function. Icariin, the main active component of Epimedium koreanum, possesses many biological effects, such as improving cardiovascular function, hormone regulation, immunological function modulation, and anti-tumor activity.

Aim of the Study

This study supports the traditional use of extracts from Epimedium species in erectile dysfunction.

Materials and Methods

The Epimedium koreanum dry extract was suspended in wheat germ oil using lecithin and bee wax for oral administration. The effect of oral administration of two compositions (E-01 and E-02) standardized by their icariin content on the number of complete intromissions, the number of ejaculations, and the latent period of ejaculation (LPE) in rats were evaluated. E-01 and E-02 were administered orally for 10 days to the experimental animals. The control animals received olive oil for 10 days. On day 10, 0.5 h after the dose was administered to male rats, one virgin female rat was placed with one male rat.

Results

The number of complete intromissions increased to 23.3 ± 2.6 in the E-01 and E-02 group (dose 300 mg/kg body weight) (b.wt) and to 20.1 ± 2.3 in the E-02 group (dose 750 mg/kg b.wt) compared with 15.2 ± 2.4 in the control group of aged rats. The number of ejaculations increased from 1.1 ± 0.3 in the control-aged group to 2.6 ± 0.4 in the E-01 group. The LPE of male rats was 14.2 ± 1.8 min in the control-aged group. The LPE of the aged group was reduced to 9.8 ± 1.5 min, 9.8 ± 1.6 min, and 11.4 ± 1.8 min when treated with E-01 at a dose of 300 mg/kg b.wt, and E-02 at a dose of 300 mg/kg b.wt and 750 mg/kg b.wt, respectively.

Conclusion

It was established that oral administration of lipid-based suspension of dry extract of Epimedium koreanum in wheat germ oil improved erectile function of aged rats.     

Effects of bulb of Fritillaria ussuriensis maxim. on angiotensin converting enzyme and vascular release of NO/cGMP in rats

The present study was designed to investigate whether the Bulbus Fritillaria shows a hypotensive effect via the angiotensin converting enzyme (ACE) inhibition and elicit NO/cGMP release in rat aortic rings. Intravenous injection of Bulbus Fritillaria water extract lowered the mean arterial pressure of anesthetized rats in a dose-dependent manner. The ACE activities were inhibited significantly by the additions of ethylacetate and butanol extracts from Bulbus Fritillaria, of which IC(50) values were 292 and 320 microg/ml, respectively. Moreover, angiotensin I-induced vasoconstriction was also strongly inhibited by the additions of ethylacetate and butanol extracts from Bulbus Fritillaria. In order to assess whether NO production was induced by Bulbus Fritillaria extracts, we directly measured NO and cGMP production levels from the aortic ring elicited by extracts of Bulbus Fritillaria. Our results showed that the hexane, butanol, and water extracts of Bulbus Fritillaria increased NO and cGMP productions in intact vascular tissue. These findings suggest that Bulbus Fritillaria extracts have a hypotensive effect in rats via the inhibition of ACE activity and direct release of NO/cGMP in the vascular tissue.     

A pilot investigation into the effect of maca supplementation on physical activity and sexual desire in sportsmen

Aims of the study

Maca (Lepidium meyenii Walp) is consumed both as a sports supplement by strength and endurance athletes, and as a natural stimulant to enhance sexual drive. However, whether or not the postulated benefits of maca consumption are of scientific merit is not yet known. The aim of the study was therefore to investigate the effect of 14 days maca supplementation on endurance performance and sexual desire in trained male cyclists.

Materials and methods

Eight participants each completed a 40 km cycling time trial before and after 14 days supplementation with both maca extract (ME) and placebo, in a randomised cross-over design. Subjects also completed a sexual desire inventory during each visit.

Results

ME administration significantly improved 40 km cycling time performance compared to the baseline test (P = 0.01), but not compared to the placebo trial after supplementation (P > 0.05). ME administration significantly improved the self-rated sexual desire score compared to the baseline test (P = 0.01), and compared to the placebo trial after supplementation (P = 0.03).

Conclusions

14 days ME supplementation improved 40 km cycling time trial performance and sexual desire in trained male cyclists. These promising results encourage long-term clinical studies involving more volunteers, to further evaluate the efficacy of ME in athletes and normal individuals and also to explore its possible mechanisms of action.