Purple-bluish tongue is associated with platelet counts,and the recurrence of epithelial ovarian cancer

OBJECTIVE: To evaluate the relationship between purple-bluish tongue and platelet counts, and further to examine their associations with the recurrence of epithelial ovarian cancer.METHODS: A total of 82 epithelial ovarian cancer patients were enrolled in this study. Cluster analysis was used for grouping patients' P_(RGB)(Red-R;Green-G; Blue-B; Average percentage of RGB, P_(RGB))values. Receiver operating characteristic(ROC)curve was performed for detecting the diagnostic standard of purple-bluish tongue. χ~2 test was used to assess the relationship between purple-bluishtongue and platelet counts, and the recurrence of epithelial ovarian cancer. The perioperative(preoperative) platelet level was examinedwith tongue image and disease recurrence.RESULTS: Tongue images were classified into two groups basing on P_(RGB) values of images by cluster analysis. The numbers of cases in cluster "1"(normal color tongue) was 16 and cluster "2"(purple-bluish tongue) was 66. Two groups of P_(RGB) values, classified by cluster analysis, were significantly correlated with vision-based tongue color recognition(Kappa = 0.852, P 0.001). ROC curve showed that the ratio of P_B to PRhad the highest diagnostic value. The sensitivity and the specificity of the ratio of P_B to P_R were 95.3% and 88.9% respectively and the optimal cut-off point was 0.71. Purple-bluish tongue was significantly correlated with increased platelet counts(P 0.001). Both the increased platelet counts(P = 0.01) and purple-bluish tongue were associated with recurrence of epithelial ovarian cancer(P 0.001).CONCLUSION: The ratio of P_B to P_R greater than 0.71 could serve as an indicator for purple-bluish tongue diagnosing used in symptom pattern identification in Traditional Chinese Medicine. Purple-bluish tongue, associated with increased platelet counts, was also closely correlated with the recurrence of epithelial ovarian cancer.     

Antiobesity and antihyperlipidemic effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats: An approach to evaluate asymmetrical temperature distribution analysis using thermography

Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated complications.Methodology: In vitro radical scavenging activity of I. coccinea was assessed using DPPH, FRAP and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue samples.The relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared camera.Results: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DPPH, FRAP and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DPPH with 71.5% inhibition, FRAP with 56.8%, H2 O2 with 33% activity at 100 μg/mL concentration.Triton X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC,LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid,AST, ALT, ALP due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature(2%–5%) was observed after treatment.Conclusion: Thus, the results indicated that I. coccinea can be a drug of choice to decrease the risk of complications associated with hyperlipidemia and obesity.     

Antiobesity and antihyperlipodemic effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats: An approach to evaluate asymmetrical temperature distribution analysis using thermography

Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated complications. Methodology: In vitro radical scavenging activity of I. coccinea was assessed using DPPH, FRAP and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue samples. The relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared camera. Results: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DPPH, FRAP and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DPPH with 71.5% inhibition, FRAP with 56.8%, H2O2 with 33% activity at 100?μg/mL concentration. Triton X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC, LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid, AST, ALT, ALP due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature (2% – –5%) was observed after treatment. Conclusion: Thus, the results indicated that I. coccinea can be a drug of choice to decrease the risk of complications associated with hyperlipidemia and obesity.     

THE CLINICAL & EXPERIMENTAL OBSERVATION ON THE EFFECT OF AURICULAR ELECTRO-ACUPUNCTURE ON THE GASTROGRAM OF HUMAN BODY

119 patients with gastroduodenal diseases were observed,clinically and experi-mentally,on the effect of electro-acupuncture at the auricular acupoint"Gastric area" on gastrogram,and the effect was compared with that of Heart area and Ear tip points.There was a significant differ-ence in the amplitudes and frequencies of gastrogram of patients between pre-and post-acupuncture(P<0.01),with good diphasic regulation;the effect of acupuncturing at"Gastric area" is the mostsignificant as compared with that at "Heart" and "Ear tip".The experiment demonstrates that thereis a relationship between the "Gastric area" and stomach,and indicates that there is relative specificityof "Gastric Area" to stomach.This provides the experimental foundation for the auricular acupointtherapy in clinic.     

Effect of Jianpi-yangwei decoction on gut fungi in the patients with gastric cancer undergoing chemotherapy

Background:Our previous study shows that the empirical formula of Chinese medicine Jianpi-yangwei decoction(JYD)can improve the quality of life in patients with gastric cancer undergoing chemotherapy by increasing beneficial gut bacteria and decreasing harmful bacteria.The present study aims to investigate the effect of JYD on gut fungi in patients with gastric cancer undergoing chemotherapy.Methods:A total of 73 patients with gastric cancer undergoing chemotherapy were recruited.Twenty-nine patients in the chemotherapy group were given standard chemotherapy and 44 patients in the observation group were given JYD plus standard chemotherapy.A control group(55 cases)was recruited from the healthy medical examiners.After 3 months of treatment,life-quality score was evaluated and fecal microbiota was tested by high-throughput sequencing based on the 18S rRNA gene.Results:After treatment,life-quality score in the observation group was significantly lower than that in the chemotherapy group(P<0.05).There was no significant difference between the observation and control groups’diversity and richness indices of intestinal fungi.The Chao index for intestinal fungi in the chemotherapy group was significantly lower than that in the observation group(P<0.05).There was a significant difference between the control and chemotherapy groups in the intestinal fungi according to Shannon and Simpson indices(P<0.05).Linear discriminant analysis effect size analysis showed no significant differences among the three groups,but significant difference in intestinal fungi was observed between the observation group and the chemotherapy group.At the genus level,the relative abundance of the Aspergillus genus in the observation and control groups was significantly lower(P<0.05),the relative abundance of the Cutaneotrichosporon,Galactomyces,and Ganoderma genus taxa was significantly higher compared with those in the chemotherapy group(P<0.05),and there was no significant difference between the observation group and control group.Conclusion:JYD can ameliorate chemotherapy-induced fungal dysbacteriosis in patients with gastric cancer undergoing chemotherapy and improve the quality of life of patients.     

The Change-Over of Yin-yang and Gene Regulation in Kidney Deficiency Syndromes

The present paper studies gene regulation in kidney deficiency syndromes from the simple Nephrotic Syndrome and with the principle of positive-negative regulation to control the change-over ofyin-yang, the modem molecular biological techniques can be used, such as gene chip, representational difference analysis （RDA） and gene sequence analysis, so as to investigate the inner relationship between the genes and kidney deficiency syndromes and prove the effect given by these genes on the pathophysiological status of change-over ofyin-yang in kidney deficiency syndromes. This philosophical approach and method can also be adopted for studies of the related genes in other TCM syndromes.     

Analysis on the Effect of Acupuncture in Treating Cervical Spondylosis with Different Syndrome Types

Objective: To observe the clinical effect of acupuncture in treating cervical spondylosis with different syndrome types. Methods: One hundred and seventeen patients were randomized into the treated group: (59 cases), treated with normal acupuncture, and the control group (58 cases), treated with sham acupuncture, operated once every other day, 9 times in total (in 18 days) as one therapeutic course, and a succeeding 3-month follow-up study was carried out after terminating the therapy. The efficacy of treatment was evaluated with the Northwick Park Neck Pain Questionnaire (NPQ) and Visual Analogue Scale (VAS), and the scores gained in patients with different syndrome types were analyzed with a general linear model. Results: The NPQ and VAS: scores showed a linear decreasing tendency in both groups at the time of ending treatment and the 1st month of follow-up, but showed a secondary curve increasing tendency in the 3rd month of follow-up. Multivariate analysis showed the difference was of statistical significance (P<0.05). However, the analysis through lead-in of syndrome type as an individual influencing factor showed that syndrome type exerts a significant influence on VAS score (P<0.05), but has insignificant influence on the NPQ score (P>0.05). Conclusion: Acupuncture shows: good immediate effect in treating cervical spondylosis, but its long-term effect is not satisfactory. The difference in syndrome type may have some impact on the effects of acupuncture in alleviating pain, but exerts no evident influence on the comprehensive effect.     

Pharmacokinetics of Tetramethylpyrazine Hydrochloride in Rabbits Blood after Intranasal Administration

Objective To study the pharmacokinetic behavior of tetramethylpyrazine hydrochloride(TMPH) in plasma of rabbits after intranasal administration and the relationship between absorption and dosage, furthermore, to illustrate the effects of borneol and musk used in combination with TMPH on the plasma concentration profile of TMPH in rabbits. Methods The concentration of TMPH was determined by RP-HPLC method. Coumarin was used as an internal standard. Sample preparation was carried out by extraction and precipitation with methanol. The pharmacokinetic parameters were computed by software program DAS.3.1.4. Results Blood pharmacokinetics of TMPH fitted best to a non-compartment model. After intranasal administration with single dose at 10, 20, and 40 mg/kg of TMPH, the average values of Cmax were 8.075, 16.537, and 33.115 μg/mL, and the average values of AUC0-t were 228.93, 399.273,and 728.917 mg/(L·min), respectively. Cmax of TMPH in plasma was increased by 31.136% and 38.786% compared with those without borneol and musk, and intranasal bioavailability were increased by 21.587% and 40.633% after intranasal administration of TMPH in combination with borneol, or with borneol and musk. Conclusion Borneol and musk could enhance the intranasal absorption of TMPH and increase the concentration of TMPH in blood of rabbits, especially in the early period. This work also shows the rational compatibility between borneol and musk.     

Effect of Ipomea carnea Jacq. flowers on hematological changes in toluene diisocyanate-induced inflammation in Wistar rats

AIM： To investigate the active chloroform fraction of the ethanol extract oflpomoea carnea flowers on hematological changes in toluene diisocyanate-induced inflammation in Wistar rats. METHOD： Except for the control group, all of the rats were sensitized with intranasal application of 5 μL of 10% toluene diiso- cyanate （TDI） for 7 days. One week after second sensitization, all of the rats were provoked with 5 ~tL of 5% TDI to induce airway hypersensitivity. After the last challenge, blood and bronchoalvelor lavage （BAL） fluid were collected and subjected to total and differential leucocytes count. Flash chromatography was performed on the most active chloroform fraction to isolate an individual component. RESULTS： Treatment with the ethanolic extract and its chloroform fraction at an oral dose of 200 mg·kg^ -1 showed a significant decrease in circulating neutrophil and eosinophil in blood and BAL as compared with standard dexamethasone （DEXA）. The structure of the compound obtained from chloroform fraction oflpomea carnea was elucidated as stigmast-5, 22-dien-3β-ol on the basis of spectral data analysis. CONCLUSION： The chloroform fraction was found to be more effective to suppress airway hyper reactivity symptoms, and de- creased count of both total and differential inflammatory cells.     

用主成分分析探索不同中药组分配伍干预脑缺血的基因表达模式

目的比较清开灵主要组分黄芩苷、栀子苷及其配伍干预脑缺血损伤的基因表达模式。方法12只4周龄小鼠随机分为黄芩苷组(BA组)、栀子苷组(JA组)、栀子苷+黄芩苷组(BJ组)、尼莫地平组(NM组),建立全脑缺血再灌注小鼠模型后,各组分别给予尾静脉注射黄芩苷、栀子苷、栀子苷+黄芩苷以及尼莫地平。抽提小鼠脑组织的总RNA,制备小鼠全基因组表达谱,对其进行基因本体论功能分类,构建相关系数矩阵,对差异表达的细胞黏附相关基因进行主成分分析。结果获取16463个小鼠脑测全基因组表达谱,筛选出32个差异表达的细胞黏附相关基因。主成分分析结果显示,4组的前2个主成分的累积贡献度为100%,BJ组总得分大于单一组分组,NM组对细胞黏附相关基因干预的总得分高于BA和JA组。各次检测的主成分分布显示,NM组和3个中药组均距离较远,BJ组和BA组的距离均近于JA组。结论黄芩苷、栀子苷及其配伍均能有效干预细胞黏附相关基因,配伍后的干预对黏附相关功能基因的影响大于单一组分组,其基因表达模式和黄芩苷相近。     

基于量表等级化和主成分分析法探究缺血性中风证候要素与治法间关系

目的 运用数据挖掘技术对缺血性中风治法的分布进行研究,评估其临床疗效价值。方法 检索1978年1月至2018年12月中国期刊全文数据库（China National KnowledgeInfrastructure Database,CNKI）,维普中文科技期刊数据库（China Science and Technology Periodical Database,VIP）,万方数据库（Wanfang Data）,中国生物医学文献数据库（Chinese Biomedical Literature Database,Sino Med）。基于上述四大数据库内检索有关中医药、中西医结合治疗缺血性中风临床观察、研究文献。对各个文献证候、治法、方药等信息进行规范化、等级化采集处理后建立缺血性中风证候要素与治法数据库。运用量表评价和等级化赋分方法分析证候要素和治法,通过Kendall''s tau-b相关分析、主成分分析等多种统计方法描述缺血性中风证候要素与治法之间、各证候要素间、各治法间的相关性及其分布。结果 通过异质性分析结果显示纳入文献具有同质性、可进行后续统计量的合并。本研究共纳入450条证候、治法,经统一规范化拆分后得到1 287个单一证候要素,1 562个单一治法。各证候要素与其相对应的治法（除窍闭-开窍）均具有较高的相关性,除了相对应的证候要素与治法外,痰湿-益气（-0.52）,气虚-开窍（-0.56）也具有较高的相关性。课题组以Ⅰ类,Ⅱ类,Ⅲ类由高至低代表证候和治法要素的重要性。气虚、血瘀和火热、痰湿、腑实是缺血性中风Ⅰ类证候要素;阴虚、内风为其Ⅱ类证候要素;阴虚与阳虚为其Ⅲ类证候要素;袪痰湿、清热、通腑和熄风、活血化瘀、益气是缺血性中风Ⅰ类治法,袪痰湿、清热、通腑相伴出现的可能性较大;熄风、活血化瘀、益气相伴出现的可能性较大。滋阴、理气是缺血性中风Ⅱ类治法,其关联性较高,常相伴出现。开窍、补阳、通络分别为缺血性中风Ⅲ,Ⅳ,Ⅴ类治法。结论 气虚、血瘀、痰湿、火热、腑实是缺血性中风主要证候要素,气虚血瘀、痰热腑实为其主要证候类型;袪痰湿、清热、通腑和活血化瘀、熄风、益气治法在缺血性中风治疗中占据主要地位;临床治疗缺血性中风时,袪痰湿、清热、通腑治法常联合使用,活血化瘀法常与益气熄风法联合使用,以起协同增效之力。     

中风后疲劳刍议

中风后疲劳（Post stroke fatigue PoSF）是脑卒中后常见的、独立存在的、复杂的症状。疲劳的严重程度与年龄、性别、脑损伤的严重程度或残疾程度无必然联系。PoSF的发生机制和发生因素尚不明确,治疗也缺乏相关研究,而祖国医学对疲劳很深刻的认识及对疲劳疗效确切,急性期多以实证为主,风火痰瘀和风痰瘀血多见,恢复期、后遗症期多以虚实夹杂为主,分别以＂清热熄风,化痰通络＂、＂祛邪补虚＂、＂补肝肾,益气养血＂为基础的辩证论治有广阔的前景。     

中医中风病证候的多元统计分析

目的：探讨中医中风病证侯的多元统计分析方法。方法：对大规模现场流行病学资料（3909例）,分析了克朗巴赫系数α(Cronbach α)和分半信度（split-half reliability)后,分别求出每一变量卡方值（χ^2)、概率值（P）、优势比（OR）、95％CI。在变量的相关性及多重共线性分析并结合文献的系统分析及临床经验的基础上,应用SAS软件对有显著性意义的危险因素进行回归分析（logistic)、聚类分析及主成分分析。结果：构成证侯的症状（变量）之间的多重共线性形成了复杂的多元非线性关系,多元统计分析可客观地评价各症状在证侯中所处的地位和作用。结论：多元统计分析是揭示中医证侯复杂关系的有效方法,值得深入研究。     

中风方剂数据初步分析

目的探讨中风方剂的配伍规律及其朝代分布规律,为,临床应用提供决策支持。方法筛选《中国方剂数据库》中风方剂905首。对所选中风方剂的中药名称进行信息标准化处理;用频数统计方法对方剂中单味药进行统计;用Aptiori算法对方剂分析得到核心药组,并根据成方时间将其按照朝代分类;提出方剂经典值的概念,用来表征方剂经典程度。结果中风方剂共涉及不同药味1479种、245种中医典籍;药味使用频次前3位为防风（40．22％）、当归（36．69％）和川芎（32．71％）;获得29个三元核心药团,核心药组按功效划分前三类为解表、补益、熄风;其最小支持度〉80（以频数表示）,功效以解表为主;计算得到方剂的经典值,并按照朝代分布展示;计算得到药味重现率前10的中医药典籍及它们各自的高频药,以及方剂数占全部方剂比例前10的中医药典籍。结论获得了各中医典籍中风用药的基本规律;从经典值的朝代分布得出中医学对中风研究越发系统,用药针对性越来越强;合集类中医药典籍中风方剂选取药味组方上有明显的重复性。     

从痰论治中风探析

从中风高危因素的发生和中医证候学研究,中风的临床证候学研究以及历代治疗学研究等角度论证从痰论证中风的重要性,认为从痰论治中风,注重痰浊的致病作用,更符合中医学治未病的思想,通过治痰治疗中风应引起重视。     

卒中后认知障碍病机探析

卒中后认知障碍病位主要在脑,亦涉及肝、肾、心、脾诸脏;在其病机变化中,肾精亏虚是发病的病理基础,是本病发生的根本因素;肾精亏损、脏腑虚衰以致痰瘀内生、痹阻脑窍是本病发生、发展的共性机制;痰浊、瘀血既是脏腑虚损、功能失调的病理产物,又是导致本病发生、发展的致病因素。     

浅析中风预防及治疗

中风一、二级预防措施的发展有望降低中风的发生率。了解中风的高危因素,积极治疗原发病,采取有效预防措施与介入治疗,发病后尽量4.5h内溶栓治疗是临床预防及减少中风后遗症的有效方法。     

眼针治疗中风病疗效的中医学机理探析

眼针疗法用于治疗中风病具有显著的疗效,旨在深入地挖掘眼针疗法的理论基础,认识眼与脏腑、经络的关系,从脏腑辨证论治角度、五轮学说角度、三焦学说角度探讨眼针治疗中风病针刺处方的中医理论基础,探析眼针治疗中风病疗效的中医学机理。     

中风病急性期证型分析研究

目的 分析中风病患者证型规律并指导临床治疗。方法 收集 192 例中风病发病 1 周内患者,分为脑出血组、大面积脑梗死组、中面积脑梗死组、小面积脑梗死组,采用脑血管临床辨证规范化量表统计每组各种证型出现率。结果 脑出血组、大面积脑梗死组风证、火热证、痰证出现率高;中、小面积脑梗死组阴虚阳亢证、痰证、瘀证出现率高。结论 中风病证型根据病灶大小、病性不同有一定的规律。