水苏碱对大鼠心肌缺血再灌注损伤的保护作用

目的：研究水苏碱对大鼠急性心肌缺血再灌注损伤的保护作用.方法：冠脉结扎法复制大鼠急性心肌缺血再灌注模型.测定给药后145min血清和心肌组织中乳酸脱氢酶（LDH）、肌酸激酶（CK）、肌钙蛋白（cTnT）、超氧化物歧化酶（SOD）、丙二醛（MDA）和一氧化氮（NO）.结果：水苏碱（3、6、12mg/kg,iv）可降低缺血再灌注损伤时LDH、CK活性和cTnT浓度,减少MDA含量,升高SOD活性及NO水平.结论：水苏碱对大鼠急性心肌缺血再灌注损伤具有保护作用.     

复方刺五加注射液对大鼠心肌缺血再灌注心律失常的影响

目的：观察复方刺五加注射液(CASI)对大鼠心肌缺血再灌注心律失常的保护作用。方法：采用在体大鼠开胸结扎冠状动脉前降支30 min后,松扎再灌注60 min造成心肌缺血再灌注模型,以心电图监测心律失常情况,并测定心肌组织超氧物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,丙二醛(MAD)及Ca²⁺含量变化。结果：股静脉输注CASI 25,50,100 mg·kg^-1能有效防止大鼠心肌缺血再灌注室性心律失常的发生,延迟心律失常的出现时间,缩短心律失常的持续时间,降低ST段的抬高程度,可明显提高缺血再灌注心肌组织中SOD及GSH-Px活力,降低MAD及Ca²⁺含量。结论：CASI对大鼠心肌缺血再灌注心律失常具有明显保护作用,其机制可能与提高心肌组织的抗氧化酶活性,减少自由基及Ca²⁺超负荷对心肌的氧化损伤有关。     

甘草酸二铵对大鼠心肌缺血再灌注心律失常的影响

目的观察甘草酸二铵(DG)对大鼠心肌缺血再灌注心律失常的影响.方法在大鼠心肌缺血10min再灌注30min损伤模型上,监测肢体II导联ECG.术后用分光光度法分别测定心肌组织中丙二醛(MDA)、Na -K -ATP酶、超氧化物歧化酶(SOD)及血清乳酸脱氢酶(LDH),肌酸激酶(CK)含量.结果DG能显著降低再灌注心律失常发生率及评分(P<0.01),也能明显减少心肌组织MDA含量(P<0.01),增加SOD和Na -K -ATP酶活性,还能减少心肌CK、LDH的释放(P<0.01).结论DG具有抗心肌缺血再灌注心律失常的作用,可能与降低心肌脂质过氧化和增强氧自由基清除酶的活性有关.     

金香丹对大鼠心肌缺血再灌注损伤保护作用及机制研究

目的观察金香丹对心肌缺血再灌注损伤的保护作用并探寻其机制。方法 60只SD大鼠随机分为假手术组、模型组、金香丹组。建立大鼠心肌缺血再灌注损伤模型,检测血清超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）和肌酸激酶（CK）活性,观察心肌组织形态学改变。结果与模型组比较,金香丹组SOD活性显著升高;MDA、LDH和CK均显著降低,金香丹组心肌组织形态学改变较缺血再灌注模型组轻。结论金香丹对缺血再灌注损伤的心肌有保护作用,其机制可能与增加SOD活性、稳定生物膜有关。     

丹红注射液对大鼠心肌缺血再灌注损伤保护作用的实验研究

目的 研究丹红注射液（OH）对心肌缺血再灌注损伤的保护作用。方法通过建立大鼠心肌缺血再灌注模型，研究不同剂量丹红注射液对心肌缺血再灌注损伤的保护作用。结果DH对大鼠缺血再灌注心肌梗塞范围的缩小作用呈剂量依赖性；减少缺血再灌注心肌细胞内肌酸激酶（CK）和乳酸脱氢酶（LDH）的释放；降低心肌缺血再灌注诱发的ST—T段的抬高。结论DH对大鼠心肌缺血再灌注损伤有明显保护作用。     

"瓜蒌-薤白"药对对大鼠心肌缺血再灌注损伤的保护作用

目的 观察瓜蒌薤白配伍前后对大鼠缺血再灌注损伤的保护作用.方法 在体结扎大鼠心脏冠状动脉左前降支法,观察心电图ST段变化,对心肌组织进行HE染色观察心肌坏死组织病理变化,采用TTC染色测定心肌梗死面积,并测定血清丙二醛(MDA)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)水平.结果 瓜蒌薤白配伍后对大鼠心肌缺血再灌注损伤模型,能有效对抗心电图的ST段抬高,可明显缩小心肌梗死范围、保护心肌组织、降低血清中 LDH、CK-MB 的活性和MDA 的含量,升高血清SOD 的活性.结论 瓜蒌与薤白配伍后能有效抗心肌缺血再灌注损伤.     

姜黄素对大鼠心肌缺血再灌注损伤的保护作用

目的:观察姜黄素对大鼠心肌缺血再灌注损伤的保护作用.方法:结扎大鼠冠状动脉左前降支,使心肌缺血60 min,再灌注60 min,制成急性心肌缺血再灌注损伤模型,缺血前5 min,舌下静脉推注姜黄素.再灌结束时测量心肌梗死面积,测定血清肌酸磷酸激酶(CK),乳酸脱氢酶(LDH),心肌超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶活性(GSH-Px)及心肌丙二醛(MDA)、游离脂肪酸(FFA)含量.结果:姜黄素(20,40 mg/kg)能减少心肌梗死面积,降低血清CK、LDH活性,提高心肌SOD、GSH-Px活性,减少心肌MDA、FFA含量.结论:姜黄素对心肌缺血再灌注损伤的保护作用,与减少脂质过氧化物和增加内源性抗自由基物质的产生,纠正心肌脂肪酸代谢紊乱有关.     

金香丹对大鼠心肌缺血再灌注心律失常的防护作用

目的:观察金香丹对大鼠心肌缺血再灌注心律失常的影响.方法:采用结扎左冠状动脉前降支30min后再灌注60min的方法,建立在体大鼠心肌缺血再灌注损伤模型.实验分分为五组,即假手术组、模型组、地奥心血康组、金香丹大剂量组、金香丹小剂量组.再灌注的同时观察心电图,并记录30min内各组心律失常发生率,再灌注后15min及60min时S-T段变化.测定心肌组织丙二醛(MDA)含量:血清肌酸激酶(CK)活性水平.结果:模型组较正常组再灌注心律失常发生率、再灌注15min及60min时S-T段抬高幅度、心肌组织中MDA含量、血清中CK活性均明显升高(均P＜0.01).金香丹能不同程度地降低再灌注心律失常发生率、再灌注15min及60min时S-T段抬高幅度,降低MDA、CK水平.结论:金香丹对缺血再灌注心律失常有防护作用.     

粉防己碱预处理对大鼠心肌缺血再灌注心律失常的影响

目的：观察粉防己碱（Tet）对大鼠心肌缺血再灌注心律失常的保护作用。方法：36只雄性SD大鼠随机分为：假手术纽（Sham组），缺血再灌注组（I／R组），粉防己碱处理组（Tet组）。结扎大鼠左冠状动脉前降支（LAD）30min后松开，再灌注24h建立心肌缺血再灌注模型。记录Ⅱ导联心电图观察室性早搏（PVC）、心室纤颤（VF）和室性心动过速（VT）的发生率及VT的持续时间。再灌注结束后检测血清中乳酸脱氢酶（LDH）、心肌组织超氧化物歧化酶（SOD）、丙二醛（MAD）及Ca^2＋浓度变化。结果：粉防己碱能防止缺血再灌注室性心律失常的发生，缩短心律失常的维持时间，明显增加再灌注心肌组织中SOD活性，降低LDH、MAD值及Ca^2＋浓度。结论：粉防己碱对大鼠心肌缺血再灌注心律失常有明显保护作用，可能与增强心肌组织抗氧化酶活性，减少自由基的产生及减轻Ca^2＋超负荷有关。     

葛根素预处理对大鼠心肌缺血再灌注损伤保护作用及机制的实验研究

目的：探讨葛根素对心肌缺血再灌注损伤的保护作用及机制。方法；60只SD大鼠被随机分为3组：假手术（sham）组、缺血再灌注损伤（IR）组、葛根素（PI）组。建立大鼠心肌缺血再灌注损伤模型，检测血清SOD、MDA、LDH和CK水平；观察心肌损伤的形态学改变。结果：与IR组比较，PI组SOD活力显著升高（P〈0．05），MDA、LDH和CK含量显著降低（P〈0．01），形态改变显著减轻（P〈0．05）。结论：葛根素对缺血再灌注损伤的心肌有保护作用，其机制可能与增加SOD活性、稳定生物膜有关。     

基于ICP-MS法的加味左金丸中无机元素分析

目的 通过电感耦合等离子体质谱（inductively coupled plasma mass spectrometry,ICP-MS）法建立加味左金丸中Cd、Pb、As、Hg、Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法。方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中⁷Li、²³Na、²⁴Mg、²⁷Al、⁴⁰Ca、⁴⁸Ti、⁵¹V、⁵²Cr、⁵⁵Mn、⁵⁶Fe、⁵⁸Ni、⁵⁹Co、⁶³Cu、⁶⁶Zn、⁷⁰Ga、⁷⁵As、⁷⁷Se、⁸⁶Sr以⁷²Ge作为内标;⁹⁵Mo、¹¹⁴Cd、¹¹⁸Sn、¹²¹Sb、¹³⁷Ba以¹¹⁵In作为内标;²⁰²Hg、²⁰⁵Tl、²⁰⁸Pb以²⁰⁹Bi作为内标。对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析。通过ICP-MS法进行测定。结果 26种无机元素线性的相关系数r ≥ 0.999 6,检出限为0.001~1.500μg/L,定量限为0.01~5.00 μg/L,精密度与重复性试验的RSD均小于5%,平均回收率在82.64%~106.44%,RSD均小于5%。对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、Ca、Fe 4种元素的含量比较高,均大于500 μg/g,Cd、Pb、As、Hg、Co、Li、Sb、Mo、Sn、Cr、Se、Tl的含量比较低,均小于1 μg/g。由结果可知,人体的常量元素,如Na、Mg、Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低。根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定。结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定。     

Erstaunliches Ergebnis einer Schädelakupunktur nach Yamamoto bei Hemihypsarrhythmie rechts

A girl, three and a half years old at the respective date, premature in 25th week of gestation, had been presented. On day three after birth, she had had a IVH III° right and left with severe involvement of parenchyma and other multiple complications, e. a. infant respiratory distress syndrome up to the 42nd day postpartum, and epileptic attacks with apnoeic events.     

七味红花殊胜散对缺血再灌注肝损伤的保护作用

目的::研究七味红花殊胜敬对缺血再灌注肝损伤的保护作用.方法:在大鼠肝缺血再灌注模型上,观察七味红花殊胜散对缺血再灌注肝损伤引起的血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)含量的变化.结果:七味红花殊胜散对大鼠血清MDA、SOD、GSH与再灌组比较有明显差异(P<0.05),NO含量与再灌组比较有明显差异(P<0.05).结论:七味红花殊胜散对大鼠急性缺血再灌注肝损伤具有保护作用.     

Methodology of Canine/Feline YNSA and Tail Acupuncture

BackgroundYamamoto New Scalp Acupuncture (YNSA) is a well-known acupuncture system for human patients which was developed by Dr. Yamamoto.ObjectiveMy aim was to transpose the human YNSA map onto canines and felines.MethodI started researching a canine/feline YNSA map in 2002. I investigated approximately 3,000 canines and felines for YNSA and 2,500 for Tail Acupuncture. The ratio of canines and felines was 6 to 4. I researched and found all the equivalent points by palpating animal patients on the basis of an exact Neck Diagnosis.ResultsI nearly completed the map in 2006. The points in the occipital region are incomplete and my research is still in progress. In the process of researching canine/feline YNSA points, I accidentally found the other microsystems around the tail and named it “Tail Acupuncture”.ConclusionsYNSA and Tail Acupuncture are acupuncture systems utilizing microsystems and support each other in canines and felines. The merits of YNSA and Tail Acupuncture are 1) immediate effects, such as in cases of lameness, 2) simplicity in finding the exact points to treat and 3) the scalp and the tail can be utilized separately or together as treatment zones, according to the situation.     

Chinesische Orthopädie: Teil 1: Die Zukunft der Akupunktur in der Postmoderne

The locomotor system is one of the major fields of application for acupuncture. The results of the large acupuncture studies funded by German Health Insurances from 2000 until 2006 are impressive and repressive: they restrict acupuncture application by allocation of reimbursement and create the necessity of reorientation, since acupuncture can neither be adequately described nor prescribed solely on the basis of disease entities. Regarding these disease entities, it seems rather necessary to analyse the layers of problems and formulate a hierarchy of therapeutic goals instead. Only in this way the effects of acupuncture, related techniques and further therapeutic modalities can be matched with the set of objectives. The article presents general principles and follows their application from biological aspects to implications for the locomotor system and consequences for the application of physical therapy. The manual of acupuncture focuses on therapeutic effects and objectives first, and then goes on to sketch simple therapeutic strategies and finally complex approaches within acupuncture. These may encompass acupuncture, microsystem acupuncture and complex therapeutic modes.     

Fallbericht: Patientin mit Schlafstörung

Background

Since the onset of her menopause about ten years ago, a sixty-year old patient has been suffering from hot flashes and sleep disorders causing her to become increasingly anxious and nervous. As the patient's mother had died of breast cancer, substitution of hormones was not advisable. Relaxation methods such as Yoga and Autogenic Training could only mildly alleviate the symptoms.

Objective

To effectively treat the sleep disorder, improve quality of life, lessen frequency and severity of hot flashes

Methods

Weekly sessions with ear- and body acupuncture for ten weeks

Results

After the third acupuncture session the patient reported a decrease of nightly waking episodes but suffered from acute pain in the neck as well as shoulder region. After nine therapy sessions, the patient reported a 50% decrease of nightly waking episodes. The hot flashes, however, remained unchanged.

Discussion

In this case, acupuncture significantly improved the patient's sleeping disorder. It also reduced the symptoms of anxiety and nervousness. Other menopausal symptoms such as hot flashes and dryness of mucous tissues, could not be influenced.     

Patientin mit chronisch rezidivierenden Gelenks- und Wirbelsäulenschmerzen

Background

Acupuncture as a regulative therapy is well-suited for the treatment of functional, reversible disorders. Additionally, the WHO recommends its application for pain therapy in cases of arthritis and back pain due to various causes. This case study should clarify which paths to take in the case of a multi-morbid patient suffering from chronic pains due to polyarthrosis.

Objective

Pain reduction and improvement of quality of life as well as improvement of sleep quality.

Methods

Annual treatment series consisting of 10 sessions each, one session per week.

Results

short-term pain reduction before undergoing endoprosthetic surgery; considerable improvement of general status and sleep.

Conclusions

Acupuncture may bring temporal relief in cases of chronic pain due to osteoarthritis and should therefore be applied in combination with western medicine. Acupuncture is particularly well-suited to treat the co-emergent vegetative symptoms.     

常见证候荷瘤小鼠神经内分泌免疫组织基因转录的总体特征

目的揭示常见证候荷瘤小鼠神经-内分泌-免疫组织基因转录的总体特征。方法采用小鼠标准化四诊及辨证方法，及Gene Chip Mouse Exon1．0ST Array等技术，检测H22荷瘤小鼠早期邪毒壅盛（邪毒）和气虚、中期阳气虚、中晚期气阴阳虚等4个常见证候下丘脑、垂体、肾上腺、睾丸、脾脏、胸腺、肿瘤RNA的转录与剪接。结果正常昆明种雄性小鼠下丘脑、垂体、肾上腺RNA电泳的28S峰低于18S，而睾丸、脾脏、胸腺、肿瘤相反。肿瘤发生的早期，下丘脑、垂体、肾上腺基因表达模式发生显著改变，邪毒尤甚，出现了失代偿。下丘脑28SRNA迅速抬升并持续，气虚强于邪毒。垂体RNA总量降低，28S陡降，邪毒尤甚。肾上腺RNA电泳特征类似垂体，气虚与邪毒相近，中晚期气阴阳虚尤甚。睾丸变化不明显。随着病情发展，脾脏重量持续增加，而胸腺相反，重量持续下降，蛋白合成和糖代谢下降。肿瘤组织蛋白合成与糖代谢，邪毒大于气虚。以上7个组织与正常对照组相比，有9127个基因表达发生差异、51126个外显子剪接发生差异。结论神经-内分泌-免疫网络组织基因转录的差异是荷瘤小鼠不同证候内在重要的物质基础。