维胃方对胃溃疡大鼠治疗作用的实验研究

目的：观察维胃方治疗实验性胃溃疡的疗效及其机制。方法：采用冰醋酸制备大鼠胃溃疡模型，各治疗组造模3d后予维胃方高、中、低剂量及雷尼替丁灌胃7d；并设正常组、模型组及对照组。处死大鼠检测胃液pH值、渍疡指数，测定胃黏膜前列腺素E2（PGE2）含量，并作组织病理学观察。结果：干预后雷尼替丁组、维胃方高、中、低剂量组溃疡指数明显降低，与模型组、对照组比较，差异有显著性或非常显著性意义（P〈0．05，P〈0．01）；中剂量组降低较雷尼替丁组更明显（P〈0．05）。维胃方各剂量组的溃疡抑制率均高于雷尼替丁组：雷尼替丁组、维胃方高、中、低剂量组胃液pH值明显升高，与对照组、模型组比较，差异有显著性或非常显著性意义（P〈0．05，P〈0．01）。雷尼替丁组、维胃方高、中、低剂量组PCE2含量升高，与模型组、对照组比较，差异有显著性或非常显著性意义（P〈0．05，P〈0．01）；低剂量组升高较雷尼替丁组更明显（P〈0．05）。结论：维胃方对实验性胃溃疡具有明显修复治疗作用，其机制可能通过减少胃酸分泌，增加胃黏膜PGE2含量，从而在押制攻击因子和增强防御因子两个环节发挥作用。     

朝药关苍术提取物对无水乙醇致小鼠胃黏膜损伤的影响

目的：通过实验观察研究关苍术的不同提取物对小鼠无水乙醇实验性胃黏膜损伤的保护作用。方法：采用无水乙醇小鼠急性胃溃疡模型，凤察胃黏膜损伤面积，测定胃黏膜组织中一氧化氮（NO）和前列腺素（PEG2）的含量。结果：关苍术CO2-乙醇组、C02组和正丁醇组小鼠溃疡指数明显低于乙醇模型组；关苍术CO2-乙醇组和CO2组小鼠胃组织中PGE2巨含量及关苍术CO2-乙醇组NO含量显著高于乙醇模型组。结论：关苍术不同提取物中CO2-乙醇提取部分拮抗无水乙醇所致小鼠急性胃黏膜损伤作用较好，该作用可能与增加胃黏膜中NO、PGE2含量有关。     

大黄黄连泻心汤、理中丸对幽门结扎型、醋酸涂抹型胃溃疡寒热证模型大鼠胃组织PGE2含量的影响

目的：探讨幽门结扎型及醋酸涂抹型胃溃疡寒热证模型的形成机制及寒热方剂对胃渍疡寒热证模型的基本作用机理和方证相应的客观规律。方法：采用寒热因素结合幽门结扎、醋酸涂抹法,分别建立大鼠胃溃疡寒、热证模型,采用酶联免疫法检测受试大鼠胃组织PGE2的含量。结果：幽门结扎及醋酸涂抹型胃溃疡各模型组大鼠胃组织PGE2含量均显著降低,与空白组比较具有显著性差异（P〈0．05或P〈0．01）,其中醋酸涂抹型胃溃疡模型中以寒证降低显著,与热醋和模醋比较差异显著（P〈0．05）;大黄黄连泻心汤可显著升高热性溃疡纽大鼠胃组织PGE2含量,理中丸可显著升高寒性溃疡组大鼠胃组织PGE,的含量（P〈0．05或P〈0．01）。结论：通过“方证相应”理论验证了“病证结合”的胃溃疡寒热模型复制成功,大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过升高大鼠胃组织PGE2的含量而实现。     

丹参饮抗大鼠幽门结扎型胃溃疡作用机理的实验研究

目的：观察丹参饮对幽门结扎型胃溃疡大鼠胃液及胃组织中相关指标的影响，以探讨其作用机理。方法：采用幽门结扎法复制大鼠胃溃疡模型。收集胃液，测其胃液量、总酸度、胃蛋白酶活性及一氧化氮（NO）含量：制备胃组织匀浆，测其超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果：丹参饮对幽门结扎型胃溃疡大鼠胃液量、总酸度厦胃蛋白酶活性无明显影响，但能显著增加其胃液中NO含量；能使胃组织中SOD活性明显升高．并使胃组织中MDA含量降低。结论：丹参饮抗幽门结扎型胃溃疡的作用是通过增强防御因子即增加黏膜血流量。削弱攻击因子即提高机体抗氧化作用来实现的。     

胃溃灵抗溃疡作用的实验研究

给大鼠按３．０和．６．０ｇ／ｋｇ ｉｇ胃溃灵冲剂，对应激型、幽门结乱型和醋酸型胃溃疡皆有明显的抗溃疡作用，对醋酸型胃溃疡亦有明显的治疗作用，对幽门结扎型胃液酸度、胃蛋白酶活性。出血程度具有明显抑制作用。对预先用阿斯匹林处理的大鼠胃液中的前列腺素Ｅ２（ＰＧＥ２）含量具有明显的升高作用。毒理实验表明胃溃灵冲剂安全无毒。     

金不换总碱对幽门结扎型胃溃疡大鼠模型的作用及机理研究

目的:探讨金不换总碱(JB-2)对幽门结扎型胃溃疡大鼠模型的作用及作用机理。方法:SD大鼠60只,随机分为6组,分别为假手术组、模型组、三九胃泰胶囊组270 mg/kg及金不换总碱15、30、60 mg/kg组。各组预防性灌胃给药7 d后,除假手术组外通过幽门结扎法制备大鼠急性胃溃疡模型,后再十二指肠给药1次,收集胃液并检测胃液量、pH值、总酸度及胃蛋白酶活性;剖开胃进行胃黏膜溃疡指数测定;测定血清和胃组织中丙二醛(MDA)含量、超氧化物岐化酶(SOD)活性;测定血清中前列腺素E2(PGE2)含量。结果:与模型组比较,金不换总碱可减少胃溃疡大鼠模型的胃液量、升高胃液pH值、减少胃液总酸度、降低胃蛋白酶活性;减轻胃黏膜损伤指数,其损伤抑制率分别为:54.29%、51.43%;可降低血清和胃组织中MDA含量、升高血清和胃组织中SOD活性、血清中PGE2含量。结论:金不换总碱有明显抗胃溃疡的作用,对攻击因子胃酸、胃蛋白酶等有一定的抑制作用,对保护因子前列腺素PGE2等有明显促进作用,作用机理可能与抗氧化、抗炎和抗菌作用有关。     

溃疡平抗大鼠乙酸胃溃疡作用及其机理研究

目的:研究溃疡平对大鼠乙酸胃溃疡的影响,并对其作用机理进行初步探讨.方法:采用改良Okabe法制备慢性乙酸胃溃疡模型,实验设正常对照组、模型对照组、溃疡平高、中、低剂量组、盐酸雷尼替丁组,观察渍疡指数和溃疡抑制率的变化,测定大鼠胃液量、胃液pH、胃蛋白酶活性、血清SOD活性及MDA和NO含量.结果:溃疡平能明显降低大鼠乙酸胃溃疡的溃疡指数(P＜0.05),溃疡平低、中、高剂量组的溃疡抑制率分别达28.43%、66.72%和92.26%;明显减少胃液分泌量(P＜0.01)和降低胃蛋白酶活性(P＜0.01),降低MDA的含量(P＜0.01);亦明显升高大鼠血清NO含量(P＜0.05)和SOD活性(P＜0.01).结论:溃疡平对胃溃疡具有较好促愈合作用,其作用机理可能与减少胃液分泌及胃蛋白酶活性,提高胃粘膜抵抗氧自由基能力,促进胃粘膜保护因子NO的合成和释放有关.说明渍疡平可能通过抑制损伤因子及增强保护因子两个环节发挥抗溃疡作用.     

吴茱萸汤抗大鼠幽门结扎型胃溃疡作用机理的实验研究

目的:观察吴茱萸汤对幽门结扎型胃溃疡大鼠胃液及胃组织中相关指标的影响,以探讨其作用机理。方法:采用幽门结扎法复制大鼠胃溃疡模型。收集胃液,测其胃液量、胃液总酸度、胃蛋白酶活性、一氧化氮(NO)含量;制备胃组织匀浆,测其超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果:吴茱萸汤对幽门结扎型胃溃疡大鼠胃液量、总酸度及胃蛋白酶活性有明显的抑制作用,能显著增加其胃液中NO含量;能使胃组织中SOD活性明显升高。结论:吴茱萸汤抗幽门结扎型胃溃疡的作用是通过抑制攻击因子与促进防御因子,即抑制胃液总酸度、胃蛋白酶活性,增加粘膜血流量,提高机体抗氧化能力实现的。     

胃疡平浸膏抗胃溃疡作用研究

目的观察胃疡平浸膏对3种胃溃疡模型动物的治疗保护作用，并初步探讨作用机制。方法将动物分成生理盐水对照组（阴性对照）、胃苏冲剂组（阳性对照）、胃疡平小、中、大剂量组5组，分别灌胃给药一段时间后，按照传统造模方法诱导胃溃疡形成，然后计算溃疡面积；测定胃酸量、胃酸酸度、胃蛋白酶活性，并与对照组比较分析。结果胃疡平浸膏能抑制大鼠幽门结扎型胃溃疡、小鼠利血平型胃溃疡的形成；促进醋酸烧灼型胃溃疡的愈台；抑制胃液的分泌，降低胃液的酸度和胃蛋白酶含量。此外，该药对小鼠胃肠运动功能有很强的促进作用；能抑制冰醋酸引起小鼠扭体反应。结论胃疡平浸膏对各型胃溃疡均有不同程度的防治作用，其作用机制可能与该药物能减少溃疡鼠胃酸的分泌。     

胃溃宁胶囊药理实验研究

为探讨胃溃宁胶囊（ＷＫＮ）的作用机理，指导临床用药。方法：进行消炎痛型、慢性醋酸型、幽门结扎型和应激型胃溃疡模型的实验及安全试验。结果ＷＫＮ对４种胃溃疡模型都有极显著的抗溃疡作用（Ｐ〈０．０１），对幽门结扎型胃溃疡可降低胃总酸度（Ｐ〈０．０１）和抑制胃酶活性（Ｐ〈０．０５），ＷＫＮ无明显毒副作用。结论：ＷＫＮ对实验性胃溃疡安全有效，可用于临床，作用机理是抑制或中和胃酸，抑制国酶活性等。     

胃溃宁抗胃溃疡的研究

目的:研究胃溃宁的抗胃溃疡及抑酸作用。方法:分别采用皮下注射利血平法、shay氏结扎幽门法、乙酸烧灼法等建立大鼠胃溃疡模型,随机分组,模型对照组灌胃给予0.5%羧甲基纤维素钠10ml/kg、药物治疗组分别灌胃给予雷尼替丁50mg/kg、胃康胶囊1.8g/kg和不同剂量的胃溃宁(4.0g/kg,2.0g/kg,1.0g/kg),观察胃溃宁对大鼠胃溃疡面积、胃液分泌量、胃液总酸度及胃蛋白酶活性的影响。结果:胃溃宁4.0g/kg,2.0g/kg,1.0g/kg能明显抑制各型胃溃疡的形成,并有效减小胃溃疡面积,与模型对照组比较,有显著性差异(P<0.01或P<0.05);胃溃宁4.0g/kg,2.0g/kg对于幽门结扎型溃疡,能有效减少胃液分泌量,降低胃液分泌总酸度,抑制胃蛋白酶活性,与模型对照组比较,有明显差异(P<0.01或P<0.05)。结论:胃溃宁对大鼠胃溃疡模型有明显的抗胃溃疡作用。     

海螵蛸防治胃溃疡作用的机理探讨

本实验观察了海螵蛸对大鼠胃液分泌、胃壁结合粘液量和胃运动的影响，测定了应用海螵蛸后胃组织ｃＡＭＰ、前列腺素Ｅ２（ＰＧＥ２）和ＤＮＡ含量的变化。结果表明，海螵蛸２００ｍｇ灌胃及与胃液在体外反应均能降低胃液总酸度。药物２００ｍｇ１次／ｄ×１应用和连续应用１０日均能增加胃组织ｃＡＭＰ含量，而且用药１０日作用更明显。此外，还能增进胃粘膜ＰＧＥ２的合成。提示：海螵蛸系通过中和胃酸、加强细胞保护等作用途径实现     

甘草酸铋钾对大鼠实验性胃溃疡的作用

目的 研究甘草酸铋钾对胃溃疡的影响。方法 用醋酸性胃溃疡、应急性胃溃疡及幽门结扎性胃溃疡模型测定甘草酸铋钾对溃疡面积、胃液量、胃液酸度和胃蛋白酶的影响。结果 甘草酸铋钾能使溃疡面积和胃液量减少,降低胃液酸度和胃蛋白酶活性。结论 甘草酸铋钾能有效地治疗胃溃疡。     

愈疡散的药效学研究

目的:研究愈疡散(獐芽菜、象皮、白及等)抗实验性胃溃疡的作用.方法:采用乙酸烧灼型溃疡模型、应激性溃疡模型、乙醇致胃粘膜损伤模型、幽门结扎型胃溃疡模型观察愈疡散对各型溃疡及胃液量、胃液酸度、胃蛋白酶活性的影响.结果:与空白组相比,愈疡散可抑制溃疡的形成,降低大鼠胃溃疡指数,促进溃疡的愈合,抑制胃液分泌,降低胃液酸度,有降低胃蛋白酶活性的趋势.结论:实验结果与该药临床用于治疗胃溃疡的功效相吻合.     

Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats

Baccharis dracunculifolia DC (Asteraceae), a native plant from Brazil, commonly known as ‘Alecrimdo‐campo’ is widely used in folk medicine to treat inflammation, hepatic disorders and stomach ulcers, and it is the most important botanical source of Southeastern Brazilian propolis, known as green propolis. Its essential oil is composed of non‐oxygenated and oxygenated terpenes. In this work, the effects of the essential oil obtained from the aerial parts of B. dracunculifolia on gastric ulcers were evaluated. The antiulcer assays were undertaken using the following protocols in rats: nonsteroidal antiinflammatory drug (NSAID)‐induced ulcer, ethanol‐induced ulcer, stress‐induced ulcer, and determination of gastric secretion using ligated pylorus. The treatment in the doses of 50, 250 and 500 mg/kg of B. dracunculifolia essential oil significantly diminished the lesion index, the total lesion area and the percentage of lesions in comparison with both positive and negative control groups. With regard to the model of gastric secretion a reduction of gastric juice volume and total acidity was observed, as well as an increase in the gastric pH. No sign of toxicity was observed in the acute toxicity study. Considering the results, it is suggested that the essential oil of B. dracunculifolia could probably be a good therapeutic agent for the development of new phytotherapeutic medicine for the treatment of gastric ulcer. Copyright © 2009 John Wiley & Sons, Ltd.     

Antiulcer activity of methanolic extract of Momordica charantia L. in rats

Aim of the study

Momordica charantia L. (Cucurbitaceae) commonly known as ‘bitter gourd’ is a multi purpose herb cultivated in different parts of the world for its edible fruits. The objective of the present study was to evaluate the effect of standardized methanolic extract of Momordica charantia L. fruits on gastric and duodenal ulcers.

Materials and methods

The effect was evaluated in acetic acid induced chronic gastric ulcer, pylorus ligation induced gastric ulcer, ethanol induced gastric ulcer, stress induced gastric ulcer, indomethacin induced gastric ulcer and cysteamine induced duodenal ulcer model. The extract was administered orally at two different doses of 100 mg/kg and 500 mg/kg.

Results

The healing of acetic acid induced gastric ulcer was increased by both doses of the extract. In pylorus-ligated rats, the extract showed significant decrease in ulcer index, total acidity, free acidity and pepsin content and an increase in gastric mucosal content. The extract also reduced the ulcer index in stress induced, ethanol induced and indomethacin induced gastric ulcers and cysteamine induced duodenal ulcer.

Conclusion

The methanolic extract of Momordica charantia L. fruit increases healing of gastric ulcer and also prevents development of gastric ulcers and duodenal ulcers in rats.     

Antiulcer activity of methanol fraction of Barleria lupulina Lindl. in animal models

The objective of the present study was to evaluate the gastric cytoprotective activity of the methanol extract of aerial parts of the plant Barleria lupulina Lindl (Acanthaceae) in albino rats using various models of ulcers such as drug induced ulcers, restraint ulcers, duodenal ulcers and pylorus ligated ulcers. The effect of the extract on gastric secretion and lipid peroxidation (thiobarbituric acid reacting substances TBARS) was also studied in rats. The extract at the tested dose of 200 mg/kg significantly reduced the volume of gastric juice, total acidity and the ulcer index in pylorus ligated rats. It also afforded significant protection against alcohol and indomethacin induced ulcer as well as stress induced ulceration. TBARS in the stomach of indomethacin treated rats was also reduced. In addition, it gave protection against duodenal ulcers. The study suggests that the methanol extract of aerial parts of Barleria lupulina Lindl. has a protective effect against experimental gastric and duodenal ulcers.     

愈溃宁颗粒抗胃溃疡的药效学及急性毒性试验研究

目的:探讨愈溃宁颗粒(海螵蛸、延胡索、炙黄芪、白芍、炙甘草等)(YKN)的抗胃溃疡的作用机制。方法:采用应激性刺激、幽门结扎性胃溃疡、醋酸致胃溃疡、乙醇致胃黏膜损伤大鼠模型观察YKN抗胃溃疡作用;对正常大鼠胃液分泌、胃液酸度、胃蛋白酶活性的影响及扭体法镇痛试验;小鼠一日最大耐受量。结果:与空白组相比,YKN可抵制溃疡的形成,降低大鼠胃溃疡指数,促进溃疡的愈合,抑制胃液分泌,降低胃液酸度,有降低胃蛋白酶活性,减少醋酸致痛小鼠的扭体次数的综合作用。一日最大耐受量相当于临床用量的288倍。结论:YKN具有明显抗溃疡的作用且无明显的毒副作用。     

Gastroprotective Effects of Ascaridole on Gastric Ulcer in Rats

Objective To evaluate the gastroprotective activity of ascaridole. Methods The gastroprotective effect of ascaridole was evaluated on ulcer healing in rats with acetic acid-induced chronic gastric ulcer, pylorus ligation- and Aspirin-induced gastric ulcer. Ascaridole was ig administered with the dosages of 10 and 20 mg/kg once daily for 7 d. Results Ascaridole showed the significant anti-ulcer effects. In acetic acid-induced gastric ulcer rats, the ulcer areas after 10 and 20 mg/kg of ascaridole treatment were (65.1 ± 20.0) and (50.6 ± 11.0) mm2, respectively, which were significant lower (P 0.01) than that of the control group [(116.7 ± 35.8) mm2]. For pylorus ligation model, ascaridole showed a gastric ulcer healing effect in a dose-dependent manner. Ascaridole at the dose of 20 mg/kg showed 50% ulcer protection and had a significant (P 0.05) gastroprotective activity since it decreased the total acidity and pepsin activity. Compared to the control group, the two dosages of ascaridole showed the significant reduction (P 0.05) in the ulcer index on Aspirin-induced ulcer. Conclusion This study provides evidence that ascaridole shows potential efficacy on the healing of gastric ulcers induced by acetic acid, Aspirin, and pylorus ligation.     

两面针总碱抗胃溃疡作用研究

目的:研究两面针总碱对实验性胃溃疡的保护作用及其机制。方法:灌服两面针总碱7天后,用无水乙醇、束缚一冷冻法和幽门结扎法造胃溃疡模型,测量溃疡指数、胃液量、游离酸、总酸度、胃蛋白酶活性以及胃粘膜MDA含量、超氧化物歧化酶(SOD)活性、NO含量。结果:两面针组能使三种模型溃疡指数降低,对胃液量、游离酸、总酸度无明显影响,可降低胃液胃蛋白酶活性,使胃粘膜MDA含量降低,SOD活性和NO含量升高。结论:两面针总碱具有抗溃疡作用。