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门脉主干壁厚检测对日本血吸虫病肝纤维化的临床意义 总被引:4,自引:1,他引:4
对117例血吸虫病患者、30例肝炎后肝硬化患者及32例健康人分别用B超检测其门脉主干壁。结果表明,血吸虫病患者门脉主干壁普遍增厚;血吸虫病肝纤维化,急者较血吸虫病非肝纤维化、肝炎后肝硬化及健康人都有显著增厚;随着血吸虫病肝纤维化I、II、III期病情加重,其门脉主干壁厚亦递增。这改变不仅与血清透明质酸、羟脯氨酸等胶原代谢测值同步,而且与肝实质纤维变、门脉分支外径等国际TDR血吸虫病超声指标检测结果高度正相关。 相似文献
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肝刺激因子对实验性免疫性肝纤维化保护作用 总被引:7,自引:0,他引:7
目的:研究肝刺激因子(HSS)对免疫性肝纤维化的作用。方法:HSS从初生小牛肝提取,动物模型采用人血清白蛋白(HSA)制备,保护组同时腹腔注射8mgHSS。结果:(1)HSS可使升高的血清转氨酶(ALT)水平、N-乙酶-β-D-氨基葡萄糖苷酶(NAG)活力及肝组织羟脯氨酸(HYP)含量下降。(2)光、电镜下见,HSS可以保护肝细胞的受损,使成纤维细胞、I型及Ⅲ型胶原增生程度明显降低。(3)HSS使贮脂细胞数目明显减少且影响其激活,并且减少了肝内HSA免疫复合物的沉积及大量浸润的T细胞。结论:HSS对免疫性肝纤维化有保护作用,其机制可能是通过免疫途径。 相似文献
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γ-干扰素基因修饰肝细胞治疗血吸虫病肝纤维化实验研究 总被引:12,自引:0,他引:12
目的探讨γ干扰素(IFNγ)基因修饰的肝细胞经脾移植抗血吸虫病肝纤维化作用。方法采用ELISA和免疫组化方法,动态分析日本血吸虫感染小鼠IFNγ和Ⅰ、Ⅲ型胶原水平及其相互关系,并将小鼠IFNγ重组腺病毒转染的肝细胞经脾移植到感染16周小鼠,分析IFNγ基因治疗血吸虫病肝纤维化的作用。结果感染4周时,小鼠IFNγ水平明显升高,在感染12周时达高峰;但在16周以后IFNγ水平明显下降,而Ⅰ、Ⅲ型胶原的合成持续增高;IFNγ基因修饰的肝细胞经脾移植后能稳定有效地表达,显著降低Ⅰ、Ⅲ型胶原的合成、沉积,减轻肝纤维化程度。结论IFNγ可能与Ⅰ、Ⅲ型胶原的合成、沉积有关;IFNγ基因治疗能有效地发挥抗血吸虫病肝纤维化作用。 相似文献
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为了研究血吸虫病肝纤维化对外周微循环的影响,我们对82例晚期血吸虫病患者甲襞微循环及肝脏B超、胶原代谢指标进行了同步观测和分析。观察对象根据临床B超影象、血清透明质酸和血清羟脯氨酸检测结果,将117例慢性或晚期血吸虫病患者分为两组:1血吸虫病性肝纤维化组(SF组),82例,其中男性68例,女性14例;2无肝纤维化慢性血吸虫病组(NSF组)35例,其中男性28例,女性7例。3健康对照组(HP组),32例,其中男性22例,女性10例。参照Yokogawa关于肝纤维化分期的标准,又将82例SF患者分别为期(SF1组)17例、组(SF2组)42例、期(SF3组)23例。检测方法和… 相似文献
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血吸虫病吡喹酮治后一年B超及血清生化的变化 总被引:1,自引:0,他引:1
为了探讨日本血吸虫病患者杀虫治疗后肝纤维化的变化,对粪检阳性日本血吸虫病患者,用吡喹酮治疗后一年,观察55例粪检复查阴性者治疗前,后腹部B超及血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)的变化,HA与PCⅢ均用RIA法。结果显示,腹部B超检查门脉Ⅱ级分支外径(D)与内径(d)比值由2.15±0.32降至1.89±0.24,治疗前、后差异有显著性(P<0.01);治疗后肝左叶长径、肝右叶长径(Ⅰ)、脾脏长径均有明显缩小(P<0.05)。与自然人群比较治后肝左叶厚度,肝右叶最大斜径、脾脏长径、脾脏指数、门脉主干内径及D/d比值仍有显著差异,治后肝纤维化血清指标HA、PCⅢ阳性率分别由35.4%和71.7%降至14.9%和22.5%,其差异均有非常显著意义。本研究提示经吡喹酮治疗后一年,肝纤维化程度有好转,但未达正常人水平。 相似文献
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目的:探讨晚期血吸虫病巨脾型患者肝脏胶原与血清肝纤维化指标之间的关系。方法:采用免疫组化LSAB法,计算机图像分析和放射免疫法测定晚期血吸虫病巨脾患者肝脏1、3、4型胶原量并计算1/3型胶夺比值,同步检测血清PC3、HA和C4量。结果:血清HA与肝脏1型胶原量,PC3和C4与1/3型胶原比值显著正相关。结论:血清PC3和C4能反映肝纤维化程度,血清肝纤维化指标与肝脏胶原病理检查对诊断肝纤维化具有互 相似文献
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目的:探讨HBV和HCV对日本血吸虫病肝纤维化的影响。方法:用放免和酶标法分别测定晚血、慢血和自然人群血清的HBSAg和抗HCV,分成HBSAg阳性组,抗HCV阳性组和双阴性组,用放免法和生化法测定其血清肝纤维化指标即透明质酸(HA)、层粘蛋白(LN)、Ⅲ型前胶原(PCⅢ)、谷胱甘肽S转移酶(GST)。结果:晚血的肝纤维化指标显著高于慢血,后者又显著高于自然人群。晚血的HBsAg和抗-HCV阳性组的HA、LN和PCⅢ均显著高于阴性组,慢血的HBsAg和抗-HCV阳性组的PCⅢ显著高于阴阳组,而HA和GST没有。结论:HBV和HCV可能有促进日本血吸虫病肝纤维化形成作用 相似文献
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目的:探讨HBV和HCV对日本血吸虫病肝纤维化的影响。方法;用放免和酶标法分别测定晚血,慢血和自然人群血清的HBSAg和抗HCV,分成HBSAg阳性组,抗HCV阳性线和双阴性组,用放免法和生化法测定其血清肝纤维化即透明质酸(HA)。层粘蛋白(LN)Ⅲ型前胶原(PCⅢ)谷胱甘肽S转移酶(GST)。结果:晚血的肝纤维化和指标显著高于慢血,后者又显著高于自然人群,晚血的HBsAg和抗-HCV阳性组的HA 相似文献
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RNA对血吸虫病肝纤维化兔血浆透明质酸和肝组织羟脯氨酸含量的影响 总被引:2,自引:0,他引:2
为探讨核糖核酸(RNA)对血吸虫病肝纤维化兔血浆透明质酸(HA)和肝组织羟脯氨酸(HYP)的影响,将54只日本家兔随机均分为正常组、病理组、RNA组。病理组和RNA组每兔感染血吸虫尾蚴(100±1)条,30d后用吡喹酮杀虫并对RNA组用RNA治疗,于用RNA后第40、70、100d,每组随机选6只兔取血和肝供检测备用。用放射免疫方法测定血浆HA和紫外分光光度法测定肝组织HYP含量。结果RNA组HA于100d时(4.66±1.35)μg/ml,显著低于相同阶段的病理组(10.16±2.52)μg/ml(P<0.01);RNA组HYP于100d时(7.24±1.24)μg/mg肝粉,亦显著低于病理组相同阶段(10.85±1.73)μg/mg肝粉(P<0.01)。表明RNA对血吸虫病肝纤维化兔血浆HA和肝组织HYP有下调作用。 相似文献
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Ahmed Maarouf Mustapha Adham Jean-Yves Scoazec Christian Partensky 《Journal of hepato-biliary-pancreatic sciences》2008,15(2):224-227
Malignant hypercalcemia without bone metastasis may result from the abnormal secretion of parathyroid hormone (PTH) or PTH-related protein (PTH-rP). We present a case of possible PTH-rP-secreting mixed hepato/cholangiocarcinoma. In July 2000, a 52-year-old woman presented with right hypochondrial pain and weight loss. Imaging procedures revealed a 10-cm lesion involving the right hepatic lobe and the hilum. Retrohepatic inferior vena caval invasion was detected by abdominal angiography. Serum calcium was elevated, while intact PTH was undetectable. The rising level of hypercalcemia rapidly became life-threatening. Medical treatment with diphosphonate and octreotide was ineffective. Chemoembolization of the right branch of the hepatic artery, preceded by right portal vein embolization, helped regain normocalcemia and improve the patient’s general condition. Extended right hepatectomy with prosthetic replacement of the vena cava and resection of the left portal vein was performed. Histopathology revealed mixed hepato/cholangiocarcinoma. Hypocalcemia appeared postoperatively but was successfully corrected. Recurrence of diffuse hepatic nodules resulted in recurrent hypercalcemia and death 3 and a half years after the surgery. This is the third case to be reported in the English-language literature. Paraneoplastic PTH-rP-induced hypercalcemia, which may be a fatal complication, can respond to arterial chemoembolization. Extensive hepatic resection may offer a good chance for survival. 相似文献
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《Expert Review of Gastroenterology & Hepatology》2013,7(4):457-459
The Americas Hepato–Pancreato Biliary Association (AHPBA) Annual 2011 Meeting provided a forum for discussion of multidisciplinary advances surrounding six tracks including the liver, biliary system, liver transplantation, pancreas, imaging and biomedical engineering and general hepato–pancreato biliary (HPB) surgical disease. The meeting and postgraduate courses attracted the largest ever attendance and participation in the AHPBA annual meeting, including field leaders and participants from across North, Central and South America who interchanged clinical and scientific knowledge, and discussed advances in technology, care and outcomes for treatment of HPB diseases. The AHPBA Foundation, established in 2010, announced progress toward support of meritorious research in HPB disease and enrichment of educational programs. HPB fellowship graduates were recognized, marking successful establishment of the AHPBA as an important body guiding HPB education and training in the USA and the Americas. 相似文献
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本文系列观察了DAB诱发肝炎、肝硬变和肝癌大鼠血中10种微量元素变化及其变化规律。给予Wistar大鼠喂养DAB饲料26周。在实验第8周(肝炎期)、18周(肝硬变期)和26周(肝癌期)分别检测6只肝炎、肝硬变和肝癌大鼠血中10种微量元素。结果显示:肝炎时仅Mn低于正常组(P<0.005);肝硬变时Mn、Ba、Fe、Si、Zn和Se均显著低于正常组(P<0.05~0.001),Cu显著高于正常组(P<0.001);肝癌时Mn、Ba、Fe、Si、Zn、Se和Mo均显著低于正常组(P<0.05~0.001),Cu和Cr显著高于正常组(P<0.001)。结果表明:1.肝病越重,微量元素变化越明显;2.Se、Zn、Mn、Si、Fe、Mo和Ba可能对肝硬变和肝癌有预防作用。 相似文献
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Yamaguchi K Chijiiwa K Torata N Kinoshita M Tanaka M 《HPB : the official journal of the International Hepato Pancreato Biliary Association》2002,4(2):75-82
Background
With progress in molecular biology, the presence of telomerase activity, P53 mutation and Ki-ras codon 12 point mutation has been reported in malignant tumours of the liver, pancreas and biliary tree. The purpose of this paper is to clarify the clinical implications of finding these three biomarkers in the peripheral blood of affected patients.Methods
Telomerase activity, P53 mutation, and Ki-ras codon 12 point mutation in the peripheral blood were examined among 86 patients with hepato pancreato biliary disease, both benign and malignant, and the results were compared with clinical findings.Results
Of 20 patients with benign conditions, only one patient with intraductal papillary adenoma showing severe dysplasia exhibited a biomarker (telomerase activity) in the peripheral blood. In total, there were 66 patients with various HPB carcinomas. Of 56 cancer patients studied pre-operatively, 16 were positive for more than one biomarker, 13 were positive for telomerase activity, 4 for P53 mutation (three at exon 7 and another at exon 8), and 2 for Kiras codon 12 point mutation (both in the second letter). Twelve of the 16 biomarker-postiive patients had stage IV disease as opposed to 23 of 40 biomarker-negative patients. The resectability rate of the cancer was 38% in positive patients and 50% in negative patients. The one-year survival rate after resection was zero in positive patients and 15% in negative patients, but the difference was not significant (P=0.65). Of 32 patients with liver metastasis at the time of the molecular examination, eight were positive and 24 negative. Of 34 patients without liver metastasis, nine were positive and 25 negative. The development of subsequent liver metastases in those without them at the start was not significantly different in those with and without biomarkers (56 vs 36%: P=0.31).Conclusions
The three novel biomarkers of the peripheral blood seemed to be of little value for screening of early malignant HPB neoplasms but may help to predict liver metastasis. 相似文献17.
目的 观察以人Midkine(MK)启动子调控单纯疱疹病毒胸苷激酶基因(HSV-TK)/丙氧鸟苷(GCV)自杀基因系统在体外对人肝癌细胞的杀伤效应.方法 以含有人MK启动子调控的HSV-TK基因的重组腺病毒分别感染体外培养的甲胎蛋白(AFP)阳性人肝癌细胞BEL-7402和AFP阴性人肝癌细胞SMMC-7721,RT-PCR法检测HSV-TK基因在上述两种细胞中的转录表达,观察GCV对人肝癌细胞的杀伤作用.结果 GCV在体外对重组腺病毒感染的BEL-7402及SMMC-7721均有明显的杀伤作用,又以前者为著.相同的病毒滴度,其杀伤作用随着GCV浓度的增高而增强.均具有旁观者效应.结论 在体外表现HSV-TK基因的AFP阳性及阴性人肝癌细胞均可被人MK启动子调控的自杀基因HSV-TK杀伤. 相似文献
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Riyad K Chalmers CR Aldouri A Fraser S Menon K Robinson PJ Toogood GJ 《HPB : the official journal of the International Hepato Pancreato Biliary Association》2007,9(3):219-224
Objective. To assess the outcome of laparoscopic cholecystectomy on the basis of an abnormal provocative 99mtechnetium-labelled hepato imino diacetic acid (HIDA) scan for patients with typical biliary pain and normal trans-abdominal ultrasound (TUS) scan. Patients and methods. Prospective data were collected for 1201 consecutive patients with typical biliary symptoms. Patients who were found to have a normal TUS and upper GI endoscopy subsequently underwent cholescintigraphy (HIDA scan). Patients with an abnormal HIDA scan, i.e.<40% ejection fraction with Sincalide® (cholecystokinin octapeptide) – were offered cholecystectomy. Symptoms and histology were reviewed postoperatively. Results. In all, 48/1201 (4%) patients with typical biliary symptoms had a normal ultrasound and endoscopy; 35/48 patients had an abnormal provocative HIDA scan and all underwent laparoscopic cholecystectomy. Histology in all cases revealed chronic cholecystitis and 18 patients had sludge or microlithiasis within the gallbladder. At 6-week follow-up, 31 of the 35 patients were completely asymptomatic or improved. Furthermore, 79% of patients remained symptom-free or improved at a median follow-up of 28.5 months (range 4–70). Conclusions. HIDA scan is a useful clinical tool as an adjunct to the diagnosis and management of patients who present with typical biliary pain and a normal TUS scan. 相似文献
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