胃癌和癌旁组织中Fas抗原表达与细胞凋亡的关系

目的研究胃癌及癌旁组织中Ｆａｓ抗原表达与细胞凋亡的关系，探讨Ｆａｓ抗原及细胞凋亡在胃癌发病中的作用．方法采用免疫组织化学及脱氧核糖核酸末端转移酶介导的缺口末端标记（ＴＵＮＥＬ）技术对胃癌组织５０例及癌旁组织３３例中Ｆａｓ抗原表达状态及凋亡细胞进行原位观察和比较．结果胃癌组织Ｆａｓ抗原表达率为４６０％，显著低于癌旁组织的７５８％（χ２＝７２２，Ｐ＜００１）．胃癌组织凋亡细胞指数为５８％，显著低于癌旁组织的１６８％（ｔ＝８６３，Ｐ＜００１）；Ｆａｓ抗原表达阳性的胃癌和癌旁组织凋亡细胞指数显著高于Ｆａｓ抗原阴性组（ｔ＝５１６和ｔ＝３７１，Ｐ＜００１）．结论Ｆａｓ抗原对胃癌及癌旁组织中的细胞凋亡具有促进性调控作用，细胞凋亡调控异常在胃癌发病中可能起重要作用．     

肝癌患者外周血树突状细胞免疫功能低下

目的研究肝癌患者外周血树突状细胞（ＤＣ）表面免疫分子ＨＬＡＤＲ及Ｂ７表达水平及其与ＤＣ免疫功能的相关性．方法以健康成人（ｎ＝１０）作为对照，检测临床确诊中晚期肝癌（ＨＣＣ）患者（ｎ＝１０）外周血ＤＣ表面免疫分子ＨＬＡＤＲ及Ｂ７表达水平及ＤＣ免疫诱导Ｔ淋巴细胞增殖的能力．结果ＨＣＣ患者ＤＣ表面ＨＬＡＤＲ及Ｂ７表达水平（ＶＯＦ）为６７±１６及６１±１１，明显低于对照组（１０７±１４及９６±１２，Ｐ＜００５）．其ＤＣ体外诱导Ｔ细胞增殖能力（ｍｉｎ－１）为３１００±１２０，亦明显低于对照组（６２００±９０，Ｐ＜００１）．结论．ＨＣＣ患者ＤＣ表面ＨＬＡＤＲ及Ｂ７表达水平下降，并与ＤＣ免疫功能低下密切相关．     

胃癌T抗原表达及其临床意义

胃癌Ｔ抗原表达及其临床意义周晓东姜阳迟丽萍刘蕴蘅Ｓｕｂｊｅｃｔｈｅａｄｉｎｇｓｓｔｏｍａｃｈｎｅｏｐｌａｓｍｓ／ｉｍｍｕｎｏｌｏｇｙ；ｓｔｏｍａｃｈｎｅｏｐｌａｓｍｓ／ｐａｔｈｏｌｏｇｙ；ａｎｔｉｇｅｎｓ，ｖｉｒａｌ，ｔｕｍｏｒ／ａｎａｌｙｓｉｓ...     

肝硬变 肝癌患者血浆因子Ⅷ相关抗原的测定

肝硬变肝癌患者血浆因子Ⅷ相关抗原的测定吴龙奇张岩刘凯玲张虹常立传山东省交通医院济南市２５００３１ＳｕｂｊｅｃｔｈｅａｄｉｎｇｓＬｉｖｅｒｃｉｒｒｈｏｓｉｓ／ｉｍｍｕｎｏｌｏｇｙＬｉｖｅｒｎｅｏｐｌａｓｍｓ／ｉｍｍｕｎｏｌｏｇｙＶｏｎＷｉｌｅｂｒａｎ...     

Fas相关死亡区蛋白在原发性肝细胞癌中的表达及其与细胞凋亡 …

目的 研究Ｆａｓ相关死亡区（Ｆａｓ－ａｓｓｏｃｉａｔｅｄ ｄｅａｔｈ ｄｏｍａｉｎ,ＦＡＤＤ）蛋白在原发性肝细胞癌（ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ,ＨＣＣ）中的表达,并探讨其与ＨＣＣ中细胞凋亡的关系。方法 免疫组织化学方法检测ＦＡＤＤ蛋白表达,原位末端标记检测ＨＣＣ细胞凋亡。结果 １０例（２５．６％）ＨＣＣ表达ＦＡＤＤ蛋白,与癌旁非癌肝组织相比（５／８,６２．５％）,ＨＣＣ中ＦＡ     

TUNEL技术原位观察肝细胞凋亡

目的采用ＴＵＮＥＬ技术对福尔马林固定、石蜡包埋的肝组织和培养肝癌细胞进行染色，以探讨肝细胞凋亡的形态特征和分布．方法使用德国宝灵曼公司生产的原位末端标记试剂盒．石蜡包埋组织经微波处理后，行常规ＴＵＮＥＬ染色，观察凋亡细胞的形态和分布．结果ＴＵＮＥＬ阳性信号为黄绿色或黄色荧光，位于胞核，呈小圆形或环行．ＴＵＮＥＬ阳性细胞大多在形态学上表现为凋亡的细胞．对肝组织ＴＵＮＥＬＬＩ的分析显示，肝癌组织中细胞凋亡指数（０３９５）比肝硬变（００７５）和癌旁肝组织（０１０５）要高，且癌组织恶性程度越高，这种凋亡就越显著．结论改进的ＴＵＮＥＬ技术对福尔马林固定、石蜡包埋的难处理组织进行染色是一个有效的方法．     

中国肝癌临床研究进展

我国２７个省１９９０／１９９２抽样调查表明，肝癌在癌症死亡率中已上升为第二位，达２０３７／１０万，其中男性２９０１／１０万，女性１１２１／１０万［１］．我国肝癌的主要病因因素有：病毒性肝炎感染－我国肝癌患者中约９０％有乙型肝炎病毒（ＨＢＶ）感染...     

丙型肝炎核心抗原的肝癌及癌旁表达

利用丙型肝炎病毒核心抗原（ＨＣｃＡｇ）单克隆抗体、ＡＢＰＡＰ法对５５例肝癌手术标本（有癌旁）作免疫组化，结果８例阳性（１４．５４％），其中５例癌细胞有阳性信号，阳性细胞散在分布，浆呈棕色，核阴性。１例癌阳性为包涵体型，其ＨＥ相应部位为毛玻璃状（无毛玻璃者阴性）。５５例中ＨＢｓＡｇ（＋）４３例，８例ＨＣｃＡｇ（＋）中有６例ＨＢｓＡｇ亦阳性，对丙型肝炎致癌机制作了讨论。     

原发性肝癌转移与ras p53基因及其蛋白表达的关系

目的了解ｒａｓ，ｐ５３基因及其蛋白表达与原发性肝细胞癌（ＨＣＣ）转移的关系．方法应用免疫组化、狭缝杂交分别对１４例ＨＣＣ有转移者和１６例无转移者癌组织中ｒａｓ，ｐ５３基因蛋白和ＲＮＡ表达进行了研究．结果经微波修复抗原后，在１４例有转移ＨＣＣ组织中，Ｐ２１和Ｐ５３蛋白的阳性例数分别为１１例和１２例，在１６例无转移ＨＣＣ组织中，其阳性例数分别为５例和７例，两组间具有极显著性差异（Ｐ值分别为００１４和００２６）．ＨＣＣ有转移组ＮｒａｓＲＮＡ为无转移组的３～５倍，而两组间Ｐ５３ＲＮＡ量则几乎相等．结论经微波修复抗原后，Ｐ２１和Ｐ５３蛋白的免疫组化染色可作为衡量ＨＣＣ转移潜能的指标．     

FADD and TRADD expression and apoptosis in primary hepatocellular carcinoma

AIM To investigate the clinical features of FADD and TRADD expressions in primary hepatocellular carcinoma ( HCC ) and to determine their relationship with hepatic apoptosis. METHODS FADD and TRADD expressions were detected by immunohistochemistry and hepatic apoptosis were determined by in situ endlabeling ( ISEL). RESULTS Ten (25.6%) cases of HCC were detected to express FADD protein. The positive rate in HCC is lower than that in non-cancerous adjacent liver tissues (62.5%) (P＜0.05). In those of grade Ⅰ - Ⅱ, 8 (38.1%) cases were FADD positive, while only 2/18 (11. 1%) cases of grade Ⅲ - Ⅳ had detectable FADD protein (P＜0.05). No relationship was found between FADD expression and other clinical features,such as gender, age, tumor size, differentiation or metastasis. ISEL positive cells can be seen in all cases of HCC. The hepatic apoptosis was associated with FADD expression as more apoptotic cells were detected in those cases which had moderately to strongly positive FADD, as compared with negative or weak positive FADD cases (P＜ 0.05). No relationship was found between FADD expression and hepatic apoptosis in non-cancerous adjacent liver tissues. Fifteen of 39 (38.5%) cases of HCC were found positive for TRADD protein, and similar positive rate (37.5%) in non-cancerous adjacent liver tissues (P ＞0.05). The expression of TRADD is correlated with HCC differentiation,as only 22.2% of moderately to highly differentiated HCC showed positive TRADD protein, while as high as 52.4% of poorly differentiated HCC had TRADD (P＜0.05). No relationship was found between TRADD expression and gender, age, tumor size or grade or metastasis, although 42.9% of HCC of grade Ⅰ/Ⅱ showed positive TRADD which was slightly higher than that of grade Ⅲ/Ⅳ (33.3%,P ＞ 0.05). Hepatic apoptosis was not related to TRADD expression in HCC or non-cancerous adjacent liver tissues. CONCLUSION Loss of FADD expression plays an important role in HCC carcinogenesis, and expression of TRADD also contributes to HCC development. The cell apoptosis in HCC is associated with FADD expression. However, the expression of TRADD does not correlate well with hepatic apoptosis in HCC.     

Immunohistochemical detection of proliferating cell nuclear antigen in hepatocellular carcinoma

ＩｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｃａｌｄｅｔｅｃｔｉｏｎｏｆｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎｉｎｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａＷＡＮＧＤｏｎｇ１，ＳＨＩＪｉｎｇＱｕａｎ２ａｎｄＬＩＵＦｅｎｇＸｕａｎ３Ｓｕ...     

Relationship between expression of Smac and Survivin and apoptosis of primary hepatocellular carcinoma

Introduction Disturbance of the regulation of cell apoptosis can lead to cell over-proliferation, and decrease of apoptosis can result in tumorigenesis ortumor development. The apoptotic signaling pathway is regulated by a variety of factors and is based on the balance between cell death and survival factors.[1, 2] The central players in apoptosis are caspases, and one important route to activation of the caspases involves the translocation of cytochrome C (Cyt-C) from mitochondria to cytosol…     

丙型肝炎病毒核心抗原转位表达抑制肝(癌)细胞

目的探讨丙型肝炎病毒核心抗原的转位表达与肝（癌）细胞凋亡的关系及其意义．方法采用免疫组织化学方法检测ＨＣＶ核心区抗原Ｃ２２在肝硬变（ＬＣ）、肝细胞肝癌（ＨＣＣ）组织中的分布;同时对连续切片进行原位凋亡检测并计算凋亡指数．结果ＨＣＶ核心区抗原定位于肝细胞或肝癌细胞的胞质或胞核中,在ＬＣ组织中,Ｃ２２呈弥漫或灶状分布于肝细胞胞质,偶见胞核阳性的肝细胞,阳性率为６０７％;Ｃ２２抗原在ＨＣＣ细胞中以核阳性分布为主,阳性率为３７６％,癌旁肝组织以胞质阳性为多见．ＨＣＣ中ＨＣＶ核心抗原的核阳性率（７５０％,１５例／２０例）和阳性细胞数明显高于其在ＬＣ（１７７％,６例／３４例）及癌旁肝组织（１１８％,２例／１７例）中的阳性率（Ｐ＜００５）和阳性细胞数．在ＨＣＣ中,ＨＣＶ核心抗原核转位表达的癌组织的ＡＰＯＰＬＩ凋亡指数值（０１７４±００９３）显著低于胞质阳性的癌组织（０５１２±０１１３,Ｐ＜００１）．结论ＨＣＶ感染与我国ＬＣ,ＨＣＣ的发生关系密切,ＨＣＶ核心抗原在肝癌组织中常发生核转位表达,这种核转位表达使肿瘤细胞凋亡受到抑制,从而促进肿瘤组织的恶性生长．     

实验性肝细胞癌铁铜含量与MR成象的关系

目的初步阐明在MRI常规SE序列,铁、铜元素对肝细胞癌TIWI信号的影响.方法20g/LDEN灌喂Wistar大鼠(n=80),诱癌成功后行常规SE序列T1WI,T2WI扫描,对照组(n=20)不灌喂DEN.扫描后处死动物取肝组织与肝癌组织作HE、普鲁士蓝染色,并测定组织内水、铁、铜含量.结果肝癌与癌周组织含水量无显著差异,肝癌内铁含量低于癌周肝组织,肝癌内的铜含量高于癌周肝组织,在呈低信号的肝癌组织内含铁血黄素明显高于其他信号强度类型,在呈高信号的肝癌组织内明显高于其他信号强度类型的肝癌组织.结论水的含量不是影响肝癌MR表现的主要因素,肝癌内含铁血黄素在SE T1WI呈低信号起作用,肝癌内铜的蓄积对SET1WI呈高信号起作用.     

Relationship between expression of alpha-fetoprotein messenger RNA and some clinical parameters of human hepatocellular carcinoma

AIM:To certify the relationship between AFP mRNA and some pathological parameters of hepatocellular carcinoma (HCC).METHODS:We detected the expression of AFP in mRNA level in tissue samples from 52 patients suffered from HCC by RT-PCR method.RESULTS:The positive rate of AFP mRNA was 76.9% in the HCC tumor tissues,and 69.4% in the paratumor tissues from the HCC patients with severe cirrhosis.However, in HCC patients without cirrhosis, the positive rate reached 50% in tumor tissues, but no AFP mRNA expression was found in the related paratumor tissues.CONCLUSION:The AFP protein was specially expressed by HCC cells and mutated hepatocytes.The AFP mRNA was positively related with cirrhosis, but no significant relationship was found between AFP mRNA and tumor size, capsule status and tumor metastasis.