首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到19条相似文献,搜索用时 109 毫秒
1.
李翊 《国际眼科杂志》2011,11(10):1722-1723
目的:应用光学相干断层扫描仪(optical coherence tomography,OCT)检测豚鼠眼视网膜、脉络膜厚度及结构形态,探讨OCT检测豚鼠眼视网膜、脉络膜厚度与组织学检查结果的相关性。方法:对5只英国属花色豚鼠(出生后3wk)采用OCT扫描眼底并进行分析,然后处死豚鼠(10眼)行组织学检查。结果:OCT检测豚鼠视网膜厚度与组织学检查结果具备良好相关性(r=0.77,P<0.01),OCT检测视网膜厚度比组织学检查结果厚15~20μm。但豚鼠脉络膜厚度与组织学检查结果无明显相关性(r=0.19,P=0.203>0.05)。结论:豚鼠视网膜和脉络膜厚度均可通过OCT进行检测。  相似文献   

2.
黄丽娜  樊宁  成洪波  赖铭莹  赵军 《眼科》2009,18(4):239-242
目的探讨兔眼视网膜厚度的国产与进口Stratus相干光断层扫描(OCT)仪检测与组织学检查值之间的相关性。设计实验研究。研究对象15只兔眼。方法先对兔眼的视网膜检测部位进行激光标记定位,分别用国产OSE-1800型OCT实验样机和Stratus OCT扫描并测量该部位的视网膜厚度值,再采用组织学方法检测相应部位的视网膜厚度值,应用SPSS11.5软件统计学对比分析。主要指标视网膜神经上皮层厚度值。结果OSE-1800 OCT测量兔眼视网膜神经上皮层厚度平均值为(122.5±7.4)μm,Stratus OCT测量值为(118.0±5.6)μm,组织学测量值为(116.3±8.8)μm,P=0.292;OSE-1800 OCT与Stratus OCT测量兔眼视网膜神经上皮层厚度的相关系数为0.914(P=0.000),OSE-1800 OCT与组织学测量值的相关系数为0.872(P=0.001),OSE-1800 OCT与组织学测量值的相关系数为0.833(P=0.002)。结论OSE-1800型OCT与Stratus OCT均能准确地定量测量兔眼视网膜厚度,它们与组织形态学测量值均有较好的相关性。(眼科,2009,18:239-242)  相似文献   

3.
目的:观察糖尿病视网膜病变患者血管荧光素造影微动脉瘤处渗出与视网膜增厚的关系。方法:选取糖尿病视网膜病变患者24例38眼,首次及末次血管荧光素造影检查确定是否存在微动脉瘤荧光素渗出。根据毛细血管闭塞区的位置选取微动脉瘤荧光素渗出与未渗出各1眼配对进行对比。在微动脉瘤和直径为1mm区域使用光学相干断层成像术(OCT)测量视网膜厚度。结果:微动脉瘤荧光素渗出眼视网膜厚度明显高于未渗出眼(356±69μmvs318±56μm,P<0.001),渗出眼平均增长厚度高于未渗出眼(95%的可信区间为25~51μm,P<0.001).在直径为1mm区域,渗出眼视网膜厚度和平均增长厚度均明显高于未渗出眼(351±67μmvs319±59μm;最小值311±62μmvs284±60μm;最大值389±78μmvs352±66μm)。结论:血管荧光素造影显示微动脉瘤荧光素渗出可以增加视网膜厚度,并运用高分辨率OCT测量。因此,对糖尿病视网膜病变患者早期诊断微动脉瘤荧光素是否渗出可以抑制视网膜增厚。  相似文献   

4.
目的分析假性黄斑裂孔的光学相干断层扫描(optical coherence tomography,OCT)图像特征。方法收集59例(59眼)假性黄斑裂孔患者,经黄斑中心的垂直和水平线性扫描采集OCT图像,选择符合假性黄斑裂孔的图像,进行裂孔形态、视网膜前膜观察,用随机软件测量裂孔大小、裂孔中央和孔周视网膜厚度,并分析与视力的相关性。结果OCT图像表现为孔周视网膜增厚,视网膜组织内有低反射区,孔底无视网膜组织缺损及孔前盖膜,孔壁形态多样,依孔壁的形态不同分为5型:两侧均为陡峭壁型47眼;一侧陡峭;另一侧倾斜型7眼;两侧向内凹型2眼;一侧陡峭另一侧内凹型2眼;两侧均为倾斜型1眼。明确显示有视网膜前膜34眼。水平及垂直扫描裂孔中央厚度(平均113.3μm和110.0μm)与视力无明显的相关性(r2=0.013,P=0.39;r2=0.002,P=0.727)。裂孔的横径(平均476.5μm)略大于垂直径(平均446.5μm),裂孔呈横椭圆形。结论假性黄斑裂孔与全层或临界黄斑裂孔有不同的OCT图像,孔壁多为陡峭型,部分裂孔显示有视网膜前膜,裂孔中央厚度接近正常,视力下降程度轻。  相似文献   

5.
[目的]比较特发性视网膜前膜( ERM)黄斑区时域光相干断层扫描(OCT)与不同型号频域OCT扫描图像特征及定量测量结果的异同.[方法]2008年8~10月确诊为特发性ERM连续病例(ERM组)46例46只眼纳入研究.患者中,男性11例,女性35例;平均年龄(61.04±l0.13)岁.选择同期年龄、性别匹配的正常人21名21只眼作为正常对照组.两组受检者均行时域OCT(Stratus OCT)和频域OCT(Cirrus OCT、3D OCT-1000)检查.将黄斑部划分为3个同心圆,分别为直径l mm的中央区,1 mm<直径≤3 mm的内环区,3mm<直径≤6 mm的外环区.在内外环分别有2条放射线分为上、鼻、下、颞4区,共9个区.比较不同OCT视网膜成像和3种仪器测量值的差异及其相关性、正常对照组与ERM组黄斑区视网膜厚度的差异.并对ERM组黄斑区视网膜厚度与视力的相关性进行分析.[结果]时域OCT和频域OCT均可见ERM组患者黄斑区视网膜增厚,视网膜内层结构紊乱,内表面凹凸不平,内、外丛状层增生.但频域OCT对视网膜内层结构及视网膜内表面增生膜的细微病理改变显示更清晰.所有受检者黄斑区视网膜厚度,Cirrus OCT和3D OCT-1000测量值均较Stratus OCT测量值高,Cirrus OCT测量值较3D OCT-1000测量值高,差异有统计学意义0=7.445~11.253,P=0.000);3种OCT仪测量的各参数相关性良好,相关系数均大于0.9.3种OCT扫描结果均显示,ERM组黄斑中心凹凹陷变浅或消失,黄斑不同区域视网膜厚度均较正常对照组增厚,以黄斑区3 mm内环区增厚最明显,差异有统计学意义(t=2.477~10.1139,P<0.05).ERM组3种OCT仪测量的黄斑各区域视网膜厚度与视力呈中度负相关(r=-0.216~-0.517).[结论]频域OCT成像较时域OCT更清晰、精细;时域OCT与频域OCT测量值相关性较好.但并不相同,不能相互替代.  相似文献   

6.
目的 回顾性分析评价白内障超声乳化人工晶状体植入后糖尿病性视网膜病变的多波长激光治疗时机及疗效.方法 白内障术后糖尿病性视网膜病变患者35例(47只眼),所有病例均在植入人工晶状体术后二周行眼底全视网膜镜检查和FFA、OCT检查,行DR分期,第三周根据病变部位和分期,依次采用氪黄光、氪红光、氪绿光进行局灶光凝,格珊样光凝,全视网膜光凝.结果 光凝后随访3~12个月,视力提高11只眼,占23.40%,视力稳定30只眼占63.83%,视力下降6只眼占12.77%,FFA示黄斑水肿完全消退23只眼占48.94%,部分消退16只眼占34.04%,不变甚至加重8只眼占17.02%,激光治疗前OCT示黄斑部中央凹平均厚度为(390.35±102.53)μm,治疗后1个月平均厚度(287.67±82.81)μm,治疗后3个月平均厚度(208.47±56.72)μm,治疗后6个月平均厚度(168.38±42.56)μm,差异有统计学意义(P<0.05).FFA示视网膜毛细血管渗漏广泛消退或视网膜新生血管完全萎缩20只眼,无效4眼.结论 糖尿病性白内障摘除人工晶体植入术后早期进行眼底全视网膜镜、FFA、OCT检查,根据病变分期及部位采用多波长激光治疗,能有效控制白内障术后DR的进一步发展,保护视功能.  相似文献   

7.
目的 利用RTVue OCT对POAG患者黄斑区视网膜各层厚度进行分析,尤其是视神经节细胞层,探讨其在原发性开角型青光眼诊断中的价值.方法 前瞻性对照研究.临床诊断为POAG的病例76例(91眼)纳入本研究,分为早期30例(30眼),中期25例(29眼),晚期21例(32眼);选择32例(32眼)年龄、性别构成相匹配的健康体检者作为正常对照组.采用RTVue OCT对受检眼黄斑区视网膜进行扫描,用自编程序图像处理软件将视网膜结构分为9层,早、中、晚期POAG与正常对照组黄斑区视网膜各层厚度比较采用LSD-t检验.结果 早期POAG组视网膜神经纤维层(RNFL)、节细胞层(GCL)平均厚度分别为(31.6±9.2)μm、(33.9±5.0)μm,较对照组薄(P<0.05),中期POAG组RNFL、GCL平均厚度分别为(31.2±3.4)μm、(34.1±3.9)μm,较正常组薄(P<0.05);晚期POAG组RNFL、GCL、内丛状层(IPL)、锥杆细胞内节(IS)、全视网膜(TR)平均厚度分别为(18.8±7.6)μm、(24.2±7.9) μm、(38.0±6.4) μm、(22.8±4.4)μm、(299.5±15.1)μm,均较正常组薄(均P<0.05);INL平均厚度(39.1±6.6)μm,较正常组厚(P<0.05).结论 POAG患者黄斑区视网膜厚度明显变薄,早期POAG对GCL的影响尤为突出,结合临床有助于POAG的早期诊断.  相似文献   

8.
目的:应用光学相干断层成像(OCT)技术研究高度近视眼黄斑部视网膜神经上皮层体积的变化。方法:将高度近视眼68例68眼和正常对照者59例59眼分为高度近视组和对照组,OCT测量黄斑中心凹最薄处神经上皮层厚度以及后极部视网膜地形图各个区域体积,比较两组结果的差异性。结果:高度近视眼组黄斑中心凹最薄处神经上皮层厚度145.7±20.2μm,正常对照组为151.9±26.7μm,两者差异无显著性(P=0.276)。高度近视眼组黄斑部视网膜神经上皮层体积为6.761±0.579mm3,正常对照组为7.262±0.508mm3,高度近视眼组黄斑体积明显小于正常组(P=0.006)。结论:OCT能够对黄斑部神经上皮层体积进行分区精确测量,可以作为研究高度近视眼后极部视网膜变化的手段之一。  相似文献   

9.
目的应用相干光断层成像技术(OCT)测量正常眼黄斑区视网膜神经上皮层的厚度。方法应用哥白尼Soct对75只正常眼进行经黄斑中心凹的星状扫描,扫描长度7 mm,测量黄斑中心小凹,颞侧、颞上方、上方、鼻上方、鼻侧、鼻下方、下方、颞下方八个方向,分别距中心小凹距离50、150、500、1000、1500μm的视网膜神经上皮的厚度,共计3075个数据,进行统计学计算和检验。结果正常眼黄斑中心小凹的视网膜神经上皮厚度为(143.15±13.57)μm,正常眼黄斑区神经上皮厚度左右眼别之间,男女之间在1000μm处颞侧、上方、鼻侧、鼻下方、下方、颞下方的神经上皮厚度在统计学上有差异。中心小凹1000μm外鼻侧视网膜神经上皮厚度大于颞侧神经上皮厚度。结论 OCT能够精确测量正常活体黄斑视网膜厚度,正常值的测定结果有利于对黄斑疾病的诊断治疗提供客观、定量分析。  相似文献   

10.
目的对高度近视眼黄斑部进行光学相干断层扫描(Optical Coherence Tomography,OCT)检查评估。方法观察分析60例114眼OCT图象特征,测量黄斑区视网膜神经纤维层厚度,比较其与眼轴长度和矫正视力的关系。结果黄斑区视网膜神经纤维层厚度131μm~169μm,平均145μm±14μm。眼轴≤25mm时,Ⅲ组的眼数多于Ⅰ组(P<0.01);眼轴25~27mm,Ⅱ组眼数多于Ⅰ组(P<0.01);眼轴≥27mm时,Ⅰ组的眼数多于Ⅱ组(P<0.01)。视网膜神经纤维层厚度≤140μm,眼轴≥27mm的眼数多于≤25mm组(P<0.01);厚度141μm~150μm,眼轴25~27mm的眼数明显多于其它两眼轴组(均P<0.01);厚度>150μm,眼轴≤25mm的眼数多于≥27mm组(P<0.01)。厚度≤140μm,则矫正视力≤0.3的眼数多于0.4~0.5组(P<0.05);厚度141μm~150μm,0.4~0.5组的眼数多于≥0.6组(P<0.01);厚度>150μm,≥0.6组的眼数明显多于≤0.3组(P<0.01)。后巩膜葡萄肿发生率为91.23%(104眼),其中有27.88%(29眼)出现视网膜劈裂。结论OCT是高度近视眼的良好检测手段,可以观察到高度近视眼易发生的后巩膜葡萄肿和视网膜劈裂。随屈光度的增高,眼轴的增长,视网膜神经纤维层厚度逐渐变薄,矫正视力越差。  相似文献   

11.
Purpose: To evaluate the correlation of retinal thickness between optical coherence tomography (OCT) images and histologic slides.Methods: Retinal thickness was measured in 16 rabbit retinal histologic slides. The same eyes had been previously measured by OCT for the comparison of results between two methods. Retinal thickness of each OCT image section was measured using both the manually assisted (requiring localization of reflectivity peaks by observer) and automated modes of the computer software.Results: Retinal thickness measured by OCT demonstrated a high degree of correlation with retinal histologic study. The automated method (Cc =0. 66, P <0. 01) was less reliable than the manually assisted one (Cc =0. 84, P <0. 001). The former had an error in 95% confidence interval, ranged between -0.71 and 11.09μm. The latter had a less error, ranged from - 2. 99 to 5. 13μm.Conclusion: OCT can quantitatively measure the retinal thickness. However, automatical identification of the reflective boundaries by  相似文献   

12.
Retinal thickness measurements obtained using optical coherence tomography (OCT) play an essential role both in multi-center clinical trials and in normal clinical practice. Different scanning protocols are available on most OCT devices, and it is important to ascertain whether the retinal thickness measurements obtained from these are comparable. This study aimed to compare retinal thickness measurements between raster and radial scanning protocols using spectral-domain OCT (SD-OCT). In a prospective study, 32 healthy subjects were scanned sequentially using raster and radial protocols from a SD-OCT device. For both the raster and radial OCT scans, retinal thicknesses were measured manually subfoveally and at 12 other points at 0.5 mm intervals temporally and nasally on the horizontal OCT B-scan passing through the fovea. The retinal thickness measurements were compared using intraclass correlation (ICC) and Bland–Altman plots. Subfoveal retinal thickness was 227.0 µm when measured on the raster scan and 229.2 µm on the radial scan, with a mean difference of 2.2 µm (P = 0.141).The ICC for agreement was 0.889 (95 % confidence interval 0.818–0.933). Similar results were observed for retinal thickness measurements at all other points, with mean differences ranging from ?3.37 to 2.59 µm, and ICC values ranging from 0.837 to 0.972. The retinal thickness measurements obtained by the raster and radial scans of the same SD-OCT device are comparable, with differences of less than 4 µm. This is of relevance when measurements made using different OCT scan protocols are compared.  相似文献   

13.
目的:探讨光学相干断层扫描(optical coherence tomography,OCT)在视网膜中央动脉阻塞疾病中的应用及内层视网膜厚度与患者治疗前后视力是否存在相关性。

方法:回顾性分析视网膜中央动脉阻塞患者11例,收集患者发病至入院时间、初始视力、治疗后视力,行黄斑OCT测量黄斑区视网膜厚度,进行定量测定和分析黄斑中心凹、距中心小凹3mm及6mm上、下、鼻、颞侧扇形面积下视网膜全层的平均厚度。内层视网膜厚度采用人工手动测量。距黄斑中心小凹1mm处内层视网膜,鼻侧记为N1,颞侧记为T1,距黄斑中心小凹3mm处内层视网膜,鼻侧记为N3,颞侧记为T3。

结果:8/11的患者视力均有不同程度的提高; 患眼黄斑中心凹及鼻侧3mm扇形面积下视网膜全层厚度比健眼增厚(P<0.05),余各区域面积下视网膜全层厚度与健眼相比无统计学差异(P>0.05); 患眼N1、N3及T3均比健眼增厚(P<0.05); 黄斑中心凹厚度患眼与健眼的差值与治疗后视力呈负相关,r=-0.740(P<0.05); 患眼与健眼N1、T1、T3的差值与治疗后视力呈负相关,rN1=-0.692,rT1=-0.754,rT3=-0.657(P<0.05)。

结论:OCT检查对视网膜中央动脉阻塞患者治疗后视力的恢复有提示作用。  相似文献   


14.
Huang L  Fan N  Shen X  He J 《眼科学报》2011,26(3):132-137
 Purpose: To evaluate and compare the diagnostic ability of retinal nerve fiber layer (RNFL) thickness measurements using time domain (Stratus) and spectral domain (Cirrus HD) optical coherence tomography (OCT) in preperimetric and early primary open angle glaucoma (POAG). Methods: A total 62 eyes of 62 normal subjects, 47 eyes of 47 early perimetric damage POAG patients and 30 eyes of 30 preperimetric glaucoma patients were chosen in the study. All the subjects underwent peripapillary RNFL thickness measurements using Stratus OCT and Cirrus HD-OCT on the same day by a single trained operator. The RNFL thickness measured by Stratus OCT and Cirrus HD-OCT was statistically compared using paired t-tests. The relationship between RNFL thickness measured by two OCT instruments was evaluated using Pearson’s correlation coefficient. Areas under the receiver operating characteristic curves (AROC) were calculated and compared. Results: RNFL thickness measured using Stratus OCT was generally thicker than that using Cirrus HD-OCT(P<0.05). A highly significant correlation between the two OCT instruments measurements was found in four quadrants and average RNFL thickness measurements (P<0.001). The average RNFL thickness of Cirrus HD-OCT had significantly (P = 0.006) higher diagnostic ability (AROC = 0.951) than that of Stratus OCT (AROC = 0.881) in preperimetric glaucoma. There were no significant differences between the AROCs for other RNFL thickness parameters from Cirrus HD-OCT and Stratus OCT in preperimetric and early glaucoma (P>0.05). Conclusion: Significant differences and an excellent correlation were noted in terms of RNFL thickness measurements using Stratus OCT and Cirrus HD-OCT. Cirrus HD-OCT presented higher diagnostic ability for preperimetric glaucoma.  相似文献   

15.
目的 应用频域相干光断层扫描(OCT)技术评价Stargardt病患者黄斑区视网膜容积与中心视力之间的关系.方法 回顾性病例系列研究.收集10例(20只眼)Stargardt病的临床资料进行回顾性分析.所有患者均使用频域OCT分别测量黄斑中心凹区视网膜厚度、黄斑中心凹区光感受器细胞内外节缺失宽度、黄斑中心凹区直径3 mm和1 mm范围内的视网膜容积.运用线性回归分析法,比较最小分辨角对数(logMAR)视力与黄斑中心凹区视网膜厚度、黄斑中心凹区光感受器细胞内外节(IS/OS)缺失宽度、黄斑中心凹区直径3 mm和1 mm范围内视网膜容积的关系.结果 18只眼的logMAR视力为0.30~1.22,黄斑中心凹区光感受器细胞内外节缺失宽度为847~5306μm,黄斑中心凹区视网膜厚度为20~126 μm,黄斑中心凹区直径3 mm和1 mm范围内的视网膜容积分别为1.06~1.76 mm3、0.06~0.13 mm3.logMAR视力与黄斑中心凹区光感受器细胞内外节缺失宽度呈正相关(r=0.695,P<0.05),与黄斑中心凹区视网膜厚度呈负相关(r=-0.601,P<0.05),与黄斑中心凹区直径3 mm范围内的视网膜容积呈负相关(r=-0.725,P<0.05),与黄斑中心凹区直径1 mm范围内的视网膜容积无相关性(r=-0.364,P<0.05).结论 频域OCT技术可以清晰观察Stargardt 病患者各层视网膜结构,频域OCT检测获得的黄斑区视网膜容积与中心视力间存在一定的相关性.
Abstract:
Objective To assess the relationship between central visual acuity and retinal volume of macular fovea in patients with Stargardt disease by spectral-domain optical coherence tomography ( SD OCT). Methods It was a retrospective case series study. Twenty eyes of 10 patients with Stargardt disease were investigated by three-dimensional spectral-domain optical coherence tomography. SD OCT images were obtained and retrospectively analyzed. The retinal volumes of macular fovea were measured by SD OCT,whose diameters were set as 3 mm and 1 mm separately ( volume 3 and volume 1). The retinal thickness of macular fovea (macular thickness) and the width of IS/OS conjunction loss of macular fovea (IS/OS loss)were also measured by SD OCT. We correlated the logMAR BCVA with IS/OS loss, macular thickness,volume 3 and volume 1 by linear regression analysis. Results LogMAR BCVA was from 0. 3 to 1. 22.IS/OS loss was from 847 μm to 5306 μm. Macular thickness was from 20 μm to 126μm. Volume 3 and volume 1 was from 1. 06 to 1. 76 mm3 and 0. 06 to 0. 13 mm3. LogMAR BCVA correlated with the IS/OS loss (r = 0.695,P<0. 05) , macular thickness (r= - 0. 601 ,P < 0. 05), and volume 3 ( r = -0.725,P<0. 05 ). LogMAR BCVA did not correlate with volume 1 ( r = - 0. 364, P > 0. 05). Conclusions SD OCT could demonstrate the retinal structure of Stargardt disease clearly. The retinal volume of macular fovea accessed by SD OCT correlated with the visual acuity of Stargardt disease.  相似文献   

16.
目的 相干光断层扫描(OCT)观察鼠眼短暂缺血后的视网膜改变.方法 选取实验动物12只,随机作为鼠眼短暂缺血组和正常对照组(各6只),行OCT检查以观察鼠眼短暂缺血与正常鼠眼的视网膜改变.结果 短暂缺血组的6只大鼠鼠眼均显示视网膜厚度增加,神经上皮层的反射性增强;OCT检查显示鼠眼短暂缺血组平均视网膜厚度为(205.50±9.95).μm,正常鼠眼组为(186.17±3.49)μm,两者差异有统计学意义(t =4.489,P<0.01).结论 构建了鼠眼短暂缺血视网膜改变的实验模型,并由OCT观察改变情况,对今后利用动物进行相关研究具有重要意义.  相似文献   

17.
65—75岁老年人F—ERG观察   总被引:1,自引:0,他引:1  
严良  秦小玲  陆豪  周静 《眼科》2001,10(1):48-50
目的:观察65-75岁正常老年人暗适应闪光视网膜电图(F-ERG),评价其视网膜功能。方法:对41例(69只眼)正常老年人行标准化视多膜电图(F-ERG)最大反应测定,将结果与对照组(30-40岁正常人)比较;并分别比较老年人中不同性别以及(老年性白内障)不同晶状体混浊度(按WHO白内障分级)对F-ERG影响。结果:65-75岁正常老年人F-ERG最大反应中a波振幅小于对照组(0.01<P<0.05),b波振幅明显小于对照组(P<0.01);白内障眼F-ERG最大反应中a波振幅小于无白内障眼(0.01<P<0.05),b波振幅明显小于无白内障眼(P<0.01);老年男性F-ERG最大反应中a波振幅组略上于女性,但差异无显著性(P>0.1),而b波振幅明显小于女性(P<0.01)。结论:随年龄增长,正常老年人暗适应闪光视网膜电图(F-ERG)反应减弱,视网膜功能将发生生理性减退;白内障所致晶状体混浊将进一步减弱ERG反应交影响视觉功能;F-ERG最大反应存在着性别差异。  相似文献   

18.
PURPOSE: Optical coherence tomography (OCT) is a high-resolution imaging technique that measures the intensity of backscattered light from biological microstructures in living tissue. The objective was to evaluate OCT as a routine, noninvasive technique for quantitative measurements of retinal thickness and detachment in small animal models of retinal degenerative diseases. METHODS: An OCT scanning unit was designed and built to visualize retinal tissue from rodents at high resolution in vivo. Several normal and retinal degeneration (rd) mouse strains with different pigmentation, as well as a transgenic mouse strain that carries a wild-type beta-PDE gene in an rd/rd background, were analyzed at different ages. Retinal detachment was induced by subretinal injection of saline. Retinal function was evaluated by full-field ERG, and then each retina was cross-sectionally scanned by OCT. OCT image analysis and measurements of retinal thickness were performed. Animals were then killed and retinal histology was documented. RESULTS: OCT images of the mouse retina revealed structural landmarks allowing assignment of retinal structures. There was no difference in the OCT pattern between pigmented and nonpigmented mice. Changes in the retinal thickness measured by OCT correlated very well with the loss in function measured by ERG and histology in rd/rd and rd/rd/tg(+) transgenic mice at a variety of ages. In addition, retinal detachment caused by surgery was easily visualized and observed by OCT imaging. CONCLUSIONS: OCT imaging is applicable to the mouse retina. There is excellent agreement between the retinal thickness measured by OCT, ERG amplitude, and retinal histology, thus validating OCT imaging as a sensitive and noninvasive tool for monitoring the structural progression of retinal diseases in rodent models. OCT also appears useful for visualizing retinal detachments in the mouse.  相似文献   

19.
PURPOSE: To demonstrate the application of high-resolution spectral-domain optical coherence tomography (SD-OCT) for three-dimensional (3D) retinal imaging of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images. METHODS: A high-resolution SD-OCT system was built for in vivo imaging of rodent retina. OCT fundus images similar to those acquired with a scanning laser ophthalmoscope (SLO) were constructed from the measured OCT data, which provided precise spatial registration of the OCT cross-sectional images on the fundus. A 3D segmentation algorithm was developed for calculation of the retinal thickness map. OCT images were compared by histologic examination. RESULTS: High-quality OCT images of the retinas of mice (B6/SJLF2 for normal retina, rhodopsin-deficient Rho(-/-) for photoreceptor degeneration, and LH(BETA)T(AG) for retinoblastoma) and rat (Wistar) were acquired. The OCT images compared well with histology. Not only was a 3D image of the tumor in a retinoblastoma mouse model successfully imaged in vivo but the tumor volume was extracted from the 3D image. Retinal thickness maps were calculated that enabled successful quantitative comparison of the retinal thickness distribution between the normal (202.3 +/- 9.3 microm) and the degenerative (102.7 +/- 12.6 microm) mouse retina. CONCLUSIONS: High-resolution spectral-domain OCT provides unprecedented high-quality 2D and 3D in vivo visualization of retinal structures of mouse and rat models of retinal diseases. With the capability of 3D quantitative information extraction and precise spatial registration, the OCT system made possible longitudinal study of ocular diseases that has been impossible to conduct.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号