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1.
目的了解胚胎培养液中可溶性人类白细胞分化抗原-G(sHLA-G)与胚胎发育及妊娠率、种植率的关系。方法对2006年2月至5月在华中科技大学同济医学院附属同济医院行第一周期体外受精胚胎移植的不孕症患者,采用双抗体夹心ELISA,检测采卵后第3天移植的胚胎培养液中sHLA-G的表达。结果体外受精和卵母细胞单精子注射患者共139例,移植胚胎289枚。sHLA-G阳性组与阴性组的妊娠率为68.6%、10.1%,着床率分别为39.6%、6.2%,两者比较差异有统计学意义(P<0.01)。双原核一级、二级和三级胚胎的sHLA-G阳性率分别为41.9%、44.1%、7.5%,一级、二级胚胎sHLA-G阳性表达率差异无统计学意义(P>0.05),一级、二级与三级胚胎差异有统计学意义(P<0.05)。双原核4~6、7、8、9~12细胞胚胎sHLA-G阳性表达率分别为35.1%、41.0%、43.9%、36.4%,不同细胞数的胚胎sHLA-G的表达差异无统计学意义(P>0.05)。双原核和三原核受精卵发育的一级胚胎sHLA-G阳性表达率分别为43.1%、20.5%,两者差异有显著性意义(P<0.05)。双原核受精卵发育的囊胚培养液中sHLA-G阳性表达率为83.8%。结论第3天移植的胚胎培养液中sHLA-G的表达与胚胎发育有关,与妊娠率和着床率呈正相关。  相似文献   

2.
人类卵裂期胚胎sHLA-G表达与种植率和妊娠率的关系   总被引:1,自引:1,他引:0  
目的:定量测定卵裂期胚胎可溶性人白细胞抗原G(sHLA-G)的表达,探讨sHLA-G分泌与胚胎种植率、妊娠率的关系。方法:收集40例体外受精-胚胎移植(IVF-ET)助孕的80份胚胎培养液样本,用ELISA法定量检测样本中sHLA-G的表达。结果:80份胚胎培养液样本中有35份sHLA-G表达阳性(≥2U/mL),阳性率43.8%,表达阳性组的平均含量为4.68±1.29U/mL;40例患者中27例至少1个移植胚胎培养液中sHLA-G表达阳性,19例妊娠,妊娠率70.4%(19/27),所有患者均移植2枚胚胎,种植率46.2%(25/54);sHLA-G表达阴性(<2U/mL)的13例患者中3例妊娠,妊娠率23.1%(3/13),种植率15.4%(4/26),两组差异有统计学意义(P<0.01)。结论:胚胎培养液中sHLA-G含量与胚胎种植率、妊娠率有关,可用于预测胚胎的发育潜能,将sHLA-G表达水平结合形态学评分可作为选择胚胎的一种临床工具。  相似文献   

3.
目的:探讨卵泡液和卵裂起胚胎培养液中可溶性人类白细胞抗原G(sHLA-G)与卵裂期胚胎发育以及IVF-ET后临床妊娠率的关系,为临床优选卵母细胞及移植胚胎提供一种有效的非侵入性检测途径。方法:采用酶联免疫吸附试验(ELISA)检测取卵日卵泡液及第3日移植胚胎培养液中sHLA-G的表达及表达量,将40例患者分为sHLA-G表达阳性组(n=15)和sHLA-G表达阴性组(n=25)进行比较分析。结果:40份患者卵泡液sHLA-G阳性率为37.5%(15/40),87份第3日移植胚胎培养液中sHLA-G阳性率为56.32%(49/87),卵泡液和胚胎培养液sHLA-G阳性组与阴性组的获卵数和受精率均无差异(P>0.05),sHLA-G阳性组的卵裂率、第3日胚胎卵裂球数、优质胚胎率、临床妊娠率均明显高于阴性组(P<0.05),其中胚胎培养液sHLA-G阳性组的临床妊娠率显著高于阴性组(P<0.01)。结论:sHLA-G在部分卵泡液及早期胚胎培养液中均有表达,反映了胚胎良好的发育和种植潜能,预示着较好的IVF-ET结局,是一种优选卵母细胞和移植胚胎的有效的非侵入性途径。  相似文献   

4.
废弃胚胎继续囊胚培养研究   总被引:10,自引:0,他引:10  
目的:探讨体外受精治疗周期中废弃胚胎的体外发育潜能。方法:通过囊胚序贯培养法将无原核(0PN)、单个原核(1PN)、多个原核(≥3PN)和卵裂期发育延缓的2原核(2PN)废弃胚胎培养至囊胚期。比较不同来源胚胎囊胚形成情况和d3胚胎的卵裂球数、质量分级等;并利用废弃胚胎囊胚形成情况对体外受精妊娠结局进行预测。结果:共收集801个废弃胚胎,经序贯培养,形成209个囊胚(26.09%),其中58个为优质囊胚(27.75%)。1PN胚胎、0PN胚胎、d3卵裂球数为7-9-细胞胚胎、d3评分为Ⅰ-Ⅱ级胚胎以及卵裂球数为偶数的胚胎囊胚形成率较高(P均<0.05)。废弃胚胎中有囊胚形成者的临床妊娠率明显高于无囊胚形成者(P<0.05)。结论:废弃胚胎有不同程度的发育潜能,部分可发育为囊胚;特别是:①0PN和1PN胚胎;②d3的4-9-细胞胚胎,偶数卵裂球者更佳;③I级和Ⅱ级胚胎。  相似文献   

5.
目的:了解IVF和ICSI治疗周期中多原核受精卵的发育情况及其基因座遗传多态性。方法:分别收集IVF和ICSI治疗中的废弃多原核(≥3PN)受精卵315个和67个,进行体外培养,观察比较其发育能力,并复合扩增多原核来源的1株胚胎干细胞和2个囊胚细胞DNA的15个短串联重复序列(STR)基因座,利用3100遗传分析仪对其进行STR基因座多态性检测。结果:IVF组和ICSI组的多原核受精卵卵裂率无显著性差异(P>0.05),但IVF组胚胎停育率和囊胚形成率显著低于ICSI组(P<0.01)。STR基因座多态性检测显示,IVF组3PN受精卵来源的胚胎干细胞为典型的三倍体特征,但ICSI组4PN受精卵来源的2个囊胚未表现多倍体特征。结论:多原核受精卵有继续发育能力,其中IVF组多原核来源胚胎干细胞表现遗传物质倍性改变,而ICSI组多原核来源囊胚无遗传物质倍性变化。  相似文献   

6.
显微穿刺纠正多原核合子31例的临床观察   总被引:1,自引:0,他引:1  
目的 探讨多原核合子经显微穿刺纠正后的发育情况。方法 将1999年9~12月行体外受精-胚胎移植中发现的31例患者的多原核合子,在倒置显微镜下,用显微穿刺针将多原核合子中多余的原核抽出来,之后继续培养,观察其卵裂细胞数;并与同时期的正常前期胚胎相比较。结果在37℃条件下培养24h后,31例多原核合子中有17例(55%)发育到2~4细胞前期胚胎。与正常合子的分裂率94%(29/31)相比,差异有显著性(P<0.05)。但48h后两者前期胚胎的分裂速度间差异无显著性(P>0.05)。结论 用显微抽吸法纠正多原核合子可能会影响部分合子的分裂,但其余合子仍能正常发育。  相似文献   

7.
卵裂期活检对胚胎体外发育能力的影响   总被引:5,自引:0,他引:5  
Liu Q  Zhu G 《中华妇产科杂志》2002,37(5):274-277
目的 探讨不同的活检方法、活检时机和活检细胞数对胚胎体外发育能力的影响。方法 选取体外受精 胚胎移植剩余的形态学分级为Ⅰ级的胚胎 1 54个 ,对其中第 1阶段的 66个胚胎分别行化学法 (2 6个 )、机械法 (2 0个 )取出 1个卵裂球 ,并设对照 (2 0个 ) ;对第 2阶段的 88个胚胎分为用化学法取出 2个卵裂球 (44个 )和对照 (44个 )。观察记录活检时胚胎细胞数、活检时间、活检后卵裂球是否退化、活检后胚胎体外发育情况及囊胚总细胞数。结果  (1 )化学法的活检时间为 (2 31±2 0 )s,明显短于机械法的 (2 62± 2 3)s(P <0 0 1 ) ;而囊胚形成率为 65 % ,高于机械法的 35 % (P<0 0 5)。(2 ) 6 细胞胚胎的囊胚总细胞数为 (44± 4)个 ,低于 7~ 8 细胞胚胎的 (49± 5)个和≥ 9 细胞胚胎的 (50± 6)个 (P <0 0 5) ;细胞融合的≥ 9 细胞胚胎活检后不仅囊胚形成率 (2 0 % )低于对照 (67% ,P<0 0 5) ,且活检后卵裂球退化率 (50 % )高于无细胞融合的胚胎 (1 7% ,P <0 0 5)。 (3)取出 1个或 2个卵裂球胚胎的囊胚形成率和囊胚总细胞数与对照比较 ,差异均无显著性 (P >0 0 5) ,而 6 细胞胚胎取出 2个卵裂球后囊胚形成率 (1 /8)低于对照 (5/8,P <0 0 5)。结论 化学法活检比机械法更为快速、安全 ;合适的活检时  相似文献   

8.
目的探讨胚胎发育速度及形态评级并参考受精卵原核评级(联合评级),对行体外受精-胚胎移植及卵母细胞质内单精子注射后移植胚胎筛选的意义.方法回顾性分析我院2003年5-12月,采用联合评级筛选进行胚胎移植的434个周期,共2714个正常受精卵的资料.根据受精卵原核发育是否同步,分为原核发育同步组和原核发育不同步组,观察不同原核等级受精卵的发育潜力;首先根据胚胎发育速度及形态评级,再根据受精卵原核等级的联合评级选择移植胚胎.根据移植胚胎中是否含有原核发育同步的胚胎,比较原核发育同步组与原核发育不同步组受精卵进行胚胎移植后的临床妊娠率和着床率.结果2714个正常受精卵中,原核发育同步组受精卵1774个,其中优质胚胎743个,优质胚胎率为41.88%;原核发育不同步组受精卵940个,其中优质胚胎319个,优质胚胎率为33.94%,两组比较,差异有统计学意义(P<0.01).原核发育同步组中胚胎移植周期395个,临床妊娠率为47.85%(189/395),着床率为27.49%(273/993);原核发育不同步组中胚胎移植周期39个,临床妊娠率为43.59%(17/39),着床率为25.00%(21/84).两组比较,差异无统计学意义(P>0.05).结论采用联合评级,受精卵原核发育同步组与发育不同步组胚胎的临床妊娠率及着床率无差异;参考原核评级不能预测更高的妊娠率和着床率,但能预测胚胎发育的潜力.  相似文献   

9.
目的:探讨体外受精(IVF)中异常的3原核(PN)胚胎的发育及可利用价值。方法:收集IVF治疗周期中废弃的3PN受精卵204个进行体外培养,观察其发育能力,并与同周期的1 138个2PN受精卵进行比较;采用胚胎植入前遗传学筛查(PGS)技术对由3PN发育成的19枚囊胚进行非整倍体分析。结果:3PN组和2PN组的卵裂率无统计学差异(P0.05);但3PN组囊胚形成率显著低于2PN组[9.6%(19/97)vs 37.9%(204/342),P0.01]。整倍体分析显示,10.5%(2/19)的3PN来源的囊胚为正常二倍体核型。结论:3PN受精卵有继续发育能力;囊胚培养和高通量测序可作为有效筛选异常PN受精卵中正常核型胚胎的一种方法。  相似文献   

10.
目的:探讨卵裂期胚胎可溶性人类白细胞抗原G(sHLA-G)表达与胚胎发育和种植的关系。方法:收集受精后第2日和第3日的单胚胎培养液300份,应用流式微球技术(CBA)检测其中sHLAG的含量,并设置阴性对照(不含有胚胎的胚胎培养上清液)。妊娠组和未妊娠组移植胚胎各100枚。A组移植胚胎均为sHLA-G阳性,B组移植胚胎至少1枚为sHLA-G阴性,C组移植胚胎均为sHLAG阴性。分析单胚胎培养液sHLA-G的表达与临床妊娠率和种植率的关系。结果:1妊娠组第3日胚胎培养液中平均sHLA-G水平明显高于未妊娠组(P0.05)。2妊娠组第3日平均sHLA-G水平高于第2日(P0.05),但未妊娠组第3日平均sHLA-G水平升高不明显(P0.05)。3A组种植率最高,其次是B组,C组最低(P0.05)。4优质胚胎(≥6-细胞Ⅱ级)的平均sHLA-G水平妊娠组明显高于未妊娠组(P0.05)。结论:胚胎培养液中sHLA-G的表达水平与胚胎的发育潜能及其种植能力间存在正相关关系。若将第3日sHLA-G浓度(尤其是第2日及第3日sHLA-G浓度变化情况)和胚胎形态学参数评估联合作为选择最优移植胚胎的标准,有望为单胚胎移植提供参考数据。  相似文献   

11.
Identification of chemical markers in human embryo culture media that relate to embryo quality and implantation potential could be an invaluable tool in embryo selection for transfer. Embryonic secretion of soluble human leukocyte antigen G (sHLA-G) has been postulated to be a marker for 'embryonic competence'. This study examines sHLA-G concentrations in day 3 culture media droplets of embryos that were selected for transfer, as well as those being cryopreserved. A total of 712 embryo culture supernatants from 83 patients were assayed. Soluble HLA-G was detected in 306 of the 712 samples tested. In the 58 transfers in which at least one embryo selected for embryo transfer was positive for sHLA-G, the pregnancy rate was 64% (37/58) and the implantation rate per embryo transferred was 38%. In contrast, patients receiving only embryos that were negative for sHLA-G had both a lower pregnancy rate of 36% (9/25; P < 0.05) and a decreased implantation rate (19%; P < 0.05). Expression of sHLA-G was also related to increasing cell stage. Concentration of sHLA-G in embryo culture media was variable and in the low range (3-10 ng/ml). These data suggest an association between implantation potential and embryonic secretion of sHLA-G. The commercial assay kit utilized allowed for same day assessment of sHLA-G secretion. Addition of sHLA-G status to traditional morphological criteria may be useful as a clinical tool for embryo selection.  相似文献   

12.
目的:探讨可溶性人类白细胞抗原G(sHLA-G)与IVF临床妊娠率的关系。方法:收集IVF-ET患者体外单个培养胚胎的培养液,通过ELISA法检测培养液中sHLA-G的浓度,并计算相应移植胚胎的妊娠率。结果:在41名患者的84份胚胎培养液中,sHLA-G阳性率52%(44/84),阳性培养液中sHLA-G浓度为3.7 ̄16.6ng/ml;移植至少一个sHLA-G阳性胚胎的妊娠率为69%,移植胚胎全部为sHLA-G阴性的妊娠率为18%,两者相比有统计学差异(P<0.05)。结论:以sHLA-G阳性表达水平结合传统胚胎形态学评估方法,可以作为辅助选择移植胚胎的临床指标,预测IVF妊娠成功率。  相似文献   

13.
A time-lapse system was used to study the timing and coordination of events during early development from zygote to cleavage stage embryo. The aim was to identify markers linked to good-quality embryos and implantation. A total of 102 fertilized oocytes were followed for 20-24 h. Events such as appearance and disappearance of (pro)nuclei and timing and synchronization of cell cleavage were logged as time points after fertilization. Averages for these events and their synchrony were calculated and linked with fertilization method, embryo quality and implantation success. Fertilized oocytes that developed into > or =4-cell embryos had an earlier pronuclei disappearance and first cleavage than those that developed to 3- or 2-cell embryos. Intracytoplasmic sperm injection-fertilized 4-cell embryos spent a significantly shorter period as 2-cell compared with IVF-fertilized embryos (P = 0.0090). Development in the time-lapse system was similar to their siblings cultured in normal incubators, suggesting that the data from the time-lapse system can be extrapolated to the clinic's laboratory setting. Early disappearance of pronuclei and onset of first cleavage after fertilization was correlated with a higher number of blastomeres on day 2 after oocyte retrieval. In addition, synchrony in appearance of nuclei after the first cleavage was significantly associated with pregnancy success (P < 0.05).  相似文献   

14.
目的 交信号和1个Y染色体杂交信号者,则诊断为整倍体胚胎;异常杂交信号的胚胎则诊断为非整倍体胚胎.结果 (1)11个平衡易位的PGD周期中,选出杂交信号完整的130个细胞核进行分析,FISH共分析了937个荧光杂交信号,其中整倍体细胞核38个,共有304个杂交信号;其余92个为非整倍体细胞核.(2)在92个非整倍体细胞核中,Ⅰ、Ⅱ及Ⅲ级胚胎的比例分别为20个(22%)、36个(39%)及36个(39%);38个整倍体细胞核中,Ⅰ、Ⅱ及Ⅲ级胚胎的比例分别为13个(34%)、17个(45%)及8个(21%),两者的Ⅰ、Ⅱ及Ⅲ级胚胎数分别比较,差异均无统计学意义(P>0.05).虽然染色体整倍体率在不同级别胚胎中的分布比较,差异均无统计学意义(P>0.05),但优质胚胎(Ⅰ级+Ⅱ级)中非整倍体率仍为60%(56/92).(3)平衡胚胎来源的卵裂球细胞核整倍体率(71.4%,30/42)明显高于非平衡胚胎来源的卵裂球细胞核整倍体率(9.1%,8/88),两者比较,差异有统计学意义(P<0.05).平衡胚胎来源的卵裂球细胞核非整倍体率(包括三体、单体、复杂非整倍体、单倍体、多倍体)明显低于非平衡胚胎来源的卵裂球细胞核非整倍体率(P<0.05).结论 平衡易位携带者的胚胎中有较高的非整倍体率,因此,胚胎非整倍体筛查在平衡易位携带者的PGD中有重要价值和临床意义.
Abstract:
Objective To determine the importance of aneuploidy screening in preimplantation genetic diagnosis for the couples of chromosome translocation carriers. Methods To perform 11 prenatal genetic disgnosis (PGD) cycles for 7 couples of chromosome translocation carriers from January 2006 to March 2009 in the Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University. To re-analyze the well-fixed, non-multinuclear and non-debris nuclei using the probes of LSI 13, 18, 21,CEPX, CEPY to detect the aneuploidy rate which come from the PGD cycles of the couples of chromosome translocation carriers. The euploid embryo was defined as two fluorescence in situ hybridization (FISH)signals of LSI 13, 18, 21 respectively and two signals of CEPX, or one signal of CEPX and one signal of CEPY. The other abnormal signals were defined as aneuploid embryo. Results (1) A tolal of 130 nuclei from 11 PGD cycles got the integrated re-FISH signals. Nine hundred and thirty-seven FISH signals were analysized, including 304 signals from 38 euploid nuclei and the others from 92 aneuploid nuclei. (2) The number of the aneuploid nuclei from grade Ⅰ , Ⅱ and Ⅲ embryo was 20 (22%), 36(39%), and 36(39%). The number of the euploid nuclei from grade Ⅰ , Ⅱ and Ⅲ embryo was 13(34%), 17(45%),and 8(21%). There was no significant difference of aneupioidy rate between the embryos form different grades (P>0.05). However, the rate of aneuploid nucleus from good quality embryos (grade Ⅰ + grade Ⅱ) was 60% (59/92). (3) The euploidy rate was 71.4% (30/42) from balanced embryos, while 9.1%(8/88)from unbalanced embryos. There was significant difference between them (x2=53.4, P<0.05).The rate of aneuploidy from balanced embryos was lower than those from unbalanced embryos (P<0.05).Conclusions Since higher rate of aneuploidy was detected in embryos of the couples of chromosome translocation carriers. It is advisable to recommend the FISH re-analysis for aneuploidy screening to preimplantation genetic diagnosis for the couples of chromosome translocation carriers.  相似文献   

15.
The effect of polyploidy on the early development of human embryos is unknown. This study compares the early development of 90 polyploid and 275 diploid human embryos conceived in vitro. Between May 1983 and January 1986, 3081 oocytes were recovered during 631 cycles of laparoscopy for in vitro fertilization (4.9 oocytes/cycle); 1924 oocytes (62.4%) fertilized. There were 90 oocytes with more than two pronuclei (4.7% of fertilized oocytes), identified in 72 cycles (11.4% of cycles). In these cycles, the proportion of diploid oocytes (n = 275) that cleaved (cleavage rate) (92.7%) was significantly greater than the proportion of polyploid oocytes (n = 90) that cleaved (65.5%) (P less than 0.001). The cleavage rate for all diploid oocytes (n = 1834) was 90.4%. There was no significant difference in the stage of development (number of blastomeres; mean +/- standard deviation [SD]) on the day of embryo transfer between diploid (4.3 +/- 2.1) and polyploid (4.1 +/- 2.1) embryos that cleaved, but a plot of the frequency distribution of cleavage stages revealed that significantly more polyploid than diploid embryos had an uneven number of blastomeres at that time (33% versus 8%, respectively; P less than 0.001). Polyploidy confers an immediate developmental disadvantage; one third of polyploid embryos fail to cleave, and those that do divide demonstrate more asynchronous divisions.  相似文献   

16.
Extracorporeal fertilization of 363 oocytes was applied in 148 patients with tubal infertility. The aspiration of the follicles was performed 36 to 38 hrs after the onset of the endogenous LH rise or 36 hrs after injection of hCG. Oocytes maturity was evaluated on the basis of the morphological appearance of the cumulus oophorus. The eggs were incubated in culture medium before insemination. The duration of delayed insemination had a marked effect on the proportion of fertilized oocytes and the development of embryos (X2 = 10.34, P = 0.05). After maturation of 1.5 to 3.5 hrs, minimal preovulatory oocytes were fertilized and developed to embryos. Maturation of 3.5 to 5.5 hrs increased the number of fertilized oocytes from 56% to 71.7% and the number of embryos from 50% to 55.1%. After 5.5 to 7.5 hrs of maturation significantly more fertilized oocytes (t = 2.4, P less than 0.02) and developed embryos (t = 2.41, P less than 0.02) were obtained. Preincubation of intermediary preovulatory or immature oocytes had no beneficial effect on the fertilization rate and the development of embryos.  相似文献   

17.
Fifty-two women, who had 62 ovum aspiration cycles, received a progesterone (P) supplementation of 100 mg/day that was initiated 10 hours before human chorionic gonadotropin (hCG) administration and was continued over the following 6 days. Forty-eight women who had 74 ovum pick-ups, but did not get P, served as controls. Forty-four (84.6%) women of the treatment group, and 40 (83.3%) of the controls had ovum fertilization and embryo replacement. The fertilization and cleavage rates and the mean number of replaced embryos per embryo transfer (ET) cycle did not differ between the groups. Endometrial biopsies, from treatment group women with no fertilized eggs, which were taken 48 hours after ovum pick-up, mostly revealed an "advanced endometrial dating," in relation to the "day of hCG." Pregnancy rate per ET cycle for the treatment group was significantly higher than that of the controls; 41.2% versus 23.3%, respectively. It is concluded that the higher pregnancy rate resulted from an improvement in uterine receptivity.  相似文献   

18.
HLA-G and its role in implantation (review)   总被引:1,自引:0,他引:1  
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