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1.
在后基因时代,快速发展的组学技术(基因组学、转录组学、蛋白质组学、代谢组学)逐渐成为关注的焦点.代谢组学在后基因研究领域的研究对象是细胞、组织、器官的小分子化合物.代谢组学采用一些现代的分析技术和数据分析技术,在产科相关疾病的预测、发病机制的阐释、疾病的分子定向治疗上得到应用.介绍代谢组学研究中几种常用的分析技术(磁共...  相似文献   

2.
代谢组学及其在临床中的应用   总被引:3,自引:0,他引:3  
代谢组学是继基因组学、蛋白质组学、转录组学后出现的新兴"组学"。代谢组学以基因表达的最终产物(小分子代谢物)为研究对象,主要运用核磁共振、质谱、色谱等技术分离纯化得到数据,并用模式识别技术分析数据,获取有用信息。研究生物体系的代谢网络,代谢组学各单一技术平台均存在一定局限性。代谢组学是系统生物学的组成部分,在临床应用中有广阔的前景,目前代谢组学已广泛应用于疾病诊断、发病机制研究、治疗监测等领域。  相似文献   

3.
妊娠期糖尿病(GDM)易造成子痫前期、羊水过多、巨大儿等多种并发症。目前在妊娠中晚期检测血糖筛查GDM,确诊患者接受治疗的时间短,母婴健康存在严重隐患。代谢组学(metabolomics)是一种定量考察生命系统受内外界刺激或基因修饰后的代谢应答规律的学科。将代谢组学的方法应用于GDM研究,有望实现GDM的早期诊断,理解GDM的致病机制,对GDM的防治和母婴预后都有重要的意义。综述代谢组学方法在GDM及相关疾病研究中的应用。  相似文献   

4.
代谢组学采用核磁共振和质谱分析技术及数据分析手段对样本中的小分子代谢物进行分析,获得与疾病发生发展密切相关的特征性代谢物谱,对疾病的预防、诊断、治疗和预后等发挥了重要的作用。研究发现母体血浆、尿液和羊水中特征性的代谢物谱可预测胎儿畸形,母体血浆代谢组学可预测子痫前期的发生和类型,胎盘和脐血代谢组学可用于胎儿宫内缺氧和发育状况的预测,阴道代谢组学可预测早产的发生。代谢组学技术对研究孕妇和新生儿疾病的发生、发展和预后具有重要的指导意义。  相似文献   

5.
代谢组学是继基因组学、蛋白质组学、转录组学后出现的新兴"组学".代谢组学以基因表达的最终产物(小分子代谢物)为研究对象,主要运用核磁共振、质谱、色谱等技术分离纯化得到数据,并用模式识别技术分析数据,获取有用信息.研究生物体系的代谢网络,代谢组学各单一技术平台均存在一定局限性.代谢组学是系统生物学的组成部分,在临床应用中有广阔的前景,目前代谢组学已广泛应用于疾病诊断、发病机制研究、治疗监测等领域.  相似文献   

6.
代谢组学是以定量描述生物体受刺激或基因修饰后代谢物变化为目标的新兴组学,其研究对象是细胞、组织、器官的小分子化合物。妊娠期母体代谢机制是宫内环境和胎儿结局的重要决定因素,这些代谢机制发生变化可引起妊娠相关疾病,从而对妊娠结局造成影响。目前尚缺乏妊娠相关疾病的早期临床诊断方法及特异染色体疾病(如21-三体综合征)和胎儿畸形的最佳诊断方法。将代谢组学的方法应用于产科各种生理病理状态研究,有望实现妊娠相关疾病的预测及发病机制的阐释,对妊娠相关疾病的防治和母婴预后有重要意义。综述代谢组学在妊娠相关疾病中的应用及发展潜力。  相似文献   

7.
探索并找到能够成功预测卵子质量的生物标志物是提高人类辅助生殖技术(ART)成功率的关键因素之一。继形态学、基因组学、蛋白质组学之后,新近发展的代谢组学研究方法可能会成为寻找卵泡液中预测卵子质量标志物的较有前景的方法之一。代谢组学分析以其客观、准确、代谢物种类少和测量相对简单的优点成为最近的研究热点。本文就卵泡液代谢组学的研究进展做一综述。  相似文献   

8.
代谢组学是一种新的组学研究手段,其使用分析化学的检测方法和生物信息学的统计策略对生物系统(细胞、组织、生命体)中所有小分子代谢产物进行分析,任何生理或病理改变都可由代谢表型所反映,卵巢癌发生发展中表现出的代谢变化规律必然可由代谢组学研究测定,以获得系统性、整体性、动态性的信息,为卵巢癌的早期诊断、合理治疗提供理论依据.就代谢组学的概念、研究方法及其在卵巢癌中的应用做一综述.  相似文献   

9.
卵泡液代谢组学最新研究进展   总被引:2,自引:0,他引:2  
代谢组学是继基因组学、转录组学、蛋白质组学之后系统生物学的主要研究平台,并逐渐成为生命科学的研究热点。在辅助生殖技术(assisted reproductive technology,ART)领域,卵泡液代谢组学已取得较大进展。目前,国内外大多数回顾性研究表明卵泡液相关代谢物(糖类、脂肪酸、氨基酸、激素等)可预测卵母细胞发育潜能,但有待大样本、多中心随机对照试验进一步证实。卵泡液代谢组学的深入研究有望成为评估卵母细胞质量并改善ART治疗结局的重要方法和手段。  相似文献   

10.
收集最近发表的应用代谢组学技术研究多囊卵巢综合征的国内外文献,总结与多囊卵巢综合征(PCOS)发生相关的代谢物及代谢通路。从代谢组学角度探讨PCOS的发病机制及诊断、治疗进展,为PCOS的诊断和治疗提供代谢组学的依据。与PCOS发生相关的代谢物涉及碳水化合物、脂肪、蛋白质和激素;胰岛素抵抗、肥胖等与PCOS的代谢异常密切相关;药物可通过改善代谢紊乱治疗PCOS。代谢组学技术的应用有助于进一步明确PCOS的代谢紊乱,筛选的病变标志物有助于疾病的预测诊断和远期并发症的预防。  相似文献   

11.
'Omic' technologies represent a strategy towards high-throughput, simultaneous analysis of thousands of biological molecules. Their development has been accelerated in the post-genomic era since these molecules represent the interaction of genes and environment or the 'functional genome'. Omic domains are of particular interest in the search for predictive disease biomarkers and have additional relevance in understanding pathophysiology and the development of molecularly targeted therapeutics. This review examines the fields of proteomics and metabolomics in the context of obstetrics and gynaecology, including a discussion of methodology, challenges, potential applications and current research.  相似文献   

12.
卵巢癌、子宫内膜癌和宫颈癌是妇科常见的三大恶性肿瘤,尽管随着手术、放化疗等综合治疗的不断发展,妇科常见恶性肿瘤患者的预后得到了大幅提升,但由于早期没有明显症状且缺乏准确诊断或发现时已是晚期,仍有相当比例的患者预后不佳,生命受到严重威胁。因此,寻找新的治疗靶点和监测疗效的有效标志物至关重要。代谢组学(metabonomics)是肿瘤研究领域颇受关注的研究方法,其理论基础是肿瘤存在有别于正常组织的代谢特征,肿瘤独特的代谢特征与生物表型存在紧密的关联。代谢组学通过对生物系统中内源性代谢物进行全面分析,阐明肿瘤在发生和进展过程中某些关键生化途径的重塑机制,并对其进行解析与精准干预。综述代谢组学在妇科常见肿瘤治疗和预后评估中的应用,预期其可为妇科常见肿瘤的个性化、精准化治疗提供潜在靶点,为妇科常见肿瘤筛选预后生物标志物提供重要参考。  相似文献   

13.
Flow cytometry (FC) is an analytical cytology technique which has been extensively used for decades. It has many advantages compared with other similar methods for the study of cell biology, even on a molecular basis. FC allows the cell-by-cell analysis of many optical or immunological features in the same sample, at the same time, and at a rate of thousands of cells per second, generating immense quantities of data and thus providing almost limitless information which is statistically robust due to the number of units studied. The aim of this review is to describe the contribution of FC to the study of physiological and pathological processes related to human reproduction, and to discuss how this technique has been used in research, as well as its clinical applications in this field. We have used some practical examples selected from the most relevant studies within a wide range of investigations published in the literature, and we have also drawn on our own experience of using flow cytometry to study different phenomena related to reproduction. It is conclued that FC is a useful instrument for basic investigation of gynecological issues, as well as for the study of male reproductive characteristics, either in research applications or directly for clinical diagnostic purposes. Future development of these techniques will permit further advances both in our knowledge and in the improvement of assisted reproduction techniques.  相似文献   

14.
Flow cytometry in human reproductive biology.   总被引:3,自引:0,他引:3  
Flow cytometry (FC) is an analytical cytology technique which has been extensively used for decades. It has many advantages compared with other similar methods for the study of cell biology, even on a molecular basis. FC allows the cell-by-cell analysis of many optical or immunological features in the same sample, at the same time, and at a rate of thousands of cells per second, generating immense quantities of data and thus providing almost limitless information which is statistically robust due to the number of units studied. The aim of this review is to describe the contribution of FC to the study of physiological and pathological processes related to human reproduction, and to discuss how this technique has been used in research, as well as its clinical applications in this field. We have used some practical examples selected from the most relevant studies within a wide range of investigations published in the literature, and we have also drawn on our own experience of using flow cytometry to study different phenomena related to reproduction. It is conclued that FC is a useful instrument for basic investigation of gynecological issues, as well as for the study of male reproductive characteristics, either in research applications or directly for clinical diagnostic purposes. Future development of these techniques will permit further advances both in our knowledge and in the improvement of assisted reproduction techniques.  相似文献   

15.
Abstract

Background: Physiological changes leading to parturition are not completely understood while clinical diagnosis of labour is still retrospective. Gas chromatography mass spectrometry (GC/MS) and nuclear magnetic resonance spectroscopy (NMR) represent two of the main analytical platforms used in clinical metabolomics. Metabolomics might help us to improve our knowledge about the biochemical mechanisms underlying labour.

Methods: Urine samples (n?=?59), collected from pregnant women at term of gestation before and/or after the onset of labour, were analysed by GC/MS and NMR techniques in order to identify the metabolic profile. Both GC/MS and NMR data matrices containing the identified metabolites were analysed by multivariate statistical techniques in order to characterise the discriminant variables between labour (L) and not labour (NL) status.

Results: 18 potential metabolites (11 with 1H-NMR, eight with GC-MS: glycine was relevant in both) were found discriminant in urine of women during labour. Taken together, the identified metabolites produced a composite biomarker pattern, a sort of barcode, capable of differentiating between labour and not labour conditions. Major discriminant metabolites for NMR and GC/MS analysis were: alanine, glycine, acetone, 3-hydroxybutiyric acid, 2,3,4-trihydroxybutyric acid and succinic acid, giving a urine metabolite signature on the late phase of labour.

Conclusions: The metabolomics analysis evidenced clusters of metabolites involved in labour condition able to discriminate between urine samples collected before the onset and during labour, potentially offering the promise of a robust screening test.  相似文献   

16.
Abstract

Oncology is a branch of medicine in rapid evolution in the attempt to find innovative methods for early diagnosis and a better understanding of tumoral processes leading to the development of new therapies. Metabolomics is the emerging discipline among the “omics” sciences which makes it possible to further expand our knowledge concerning cancer biology. Different studies have revealed the potential role of metabolomics in gaining an understanding of pathophysiological processes in cancer, improving tumor staging, characterizing tumors and searching for biomarkers predictive of therapeutic responses. However, to date there are few works aimed at gaining deeper insights into infantile oncology through metabolomics.  相似文献   

17.
Since the first birth by IVF was achieved in 1978, the techniques involved in assisted reproductive technology have grown at an enormous rate. However, new technology has rarely been robustly validated before clinical use and developing scientific understanding of the available techniques has done little to alter their use. Furthermore, there are inconsistencies in the available clinical studies and endpoints. The benefits of some technologies already established for routine use are currently dubious and there are clear ethical concerns with providing them to patients when their scientific basis is not clear. As the uptake of assisted reproductive technology increases and newer technologies continue to push the boundaries of science, it is important to consider the clinical benefits and safety of all assisted reproductive technologies. This review will discuss aspects of some of the more recent techniques, including sperm DNA-damage tests, intracytoplasmic morphologically selected sperm injection, amino acid and metabolomics profiling, preimplantation genetic screening and time-lapse imaging, and those that may have substantial impacts on the field of reproductive medicine in the future including artificial gametes, ovarian transplantation and gene therapy.In 1978, the first child conceived by IVF was born. In the following 33 years, numerous technologies and techniques have been developed to further aid the ability to achieve pregnancies in couples for whom natural conception has failed. However, these techniques have rarely been robustly tested and approved before they are routinely offered to infertile couples. In other cases, a development in our scientific understanding of a technique has failed to be quickly incorporated into clinical changes. This raises the concern that some of the techniques offered to some patients offer little or no benefit, and in the worse cases is not confirmed to be safe. This is a particular concern as many of the techniques discussed here are often reserved for already vulnerable patients, such as those with recurrent IVF failure, This review begins by discussing some of the techniques already available to patients and questioning to what end they increase the likelihood of a live birth. The review then goes on to discuss the scientific developments that, although not currently in the clinic, could have substantial implications in the future. Since these newly developing techniques could be considered more controversial, discussion about their benefit and safety is urgently needed.  相似文献   

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