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1.
宁夏六盘山地区莱姆病调查研究   总被引:3,自引:0,他引:3  
目的:调查宁夏六盘山及周边地区莱姆病自然疫源地状况。方法:分别采用间接免疫荧光法(IFA)、ELISA法,检测人、畜血清莱姆病抗体.采集蜱类进行病原分离。结果:检测757人,抗莱姆病螺旋体IgG抗体阳性(≥1:128)24人,自然感染率3.17%,并查出典型莱姆病病例;黄牛、绵羊、山羊抗莱姆病抗体阳性水分别为23.88%(16/67)、15.82%(25/158)、20.31%(13/64)。共采集硬蜱4属5种141只,其中1属4种为宁夏首次记录;全沟硬蜱带菌率为50.0%(5/10),从80只全沟硬蜱分离出3株莱姆病疏螺旋体。结论:首次证实宁夏六盘山地两侧及前山丘陵地区、盐池县存在莱姆病自然疫源地。  相似文献   

2.
长春地区首次发现莱姆病疫源地   总被引:3,自引:0,他引:3  
目的确认长春地区是否莱姆病疫源地。方法用酶联免疫吸附试验等方法检测莱姆病人群特异IgG、IgM抗体,全沟硬蜱中肠伯氏疏螺旋体培养。长春地区全沟硬蜱733只,用BSK33℃培养,获得伯氏疏螺旋体培养物278株,用兔抗美国B31特异性单克隆抗体和针对莱姆病螺旋体种属特异M7,间接免疫荧光抗体进行试验。其结果与美国菌株稍有差异,同国内分离株M7相同。血清学调查298人份,15人阳性,平均感染率为5.03%。结果长春地区莱姆病疫源人群、家畜、动物感染莱姆病。结论首次确认长春地区存在莱姆病自然疫源地。  相似文献   

3.
目的了解当地莱姆病的主要生物媒介一蜱的种类、带菌状况以及在传播莱姆病中的作用。方法采用布旗法及动物诱捕法在山林地区采集蜱。对蜱种进行鉴定。并进行病原分离培养和PCR检测。结果共收集到1226只蜱,经鉴定属于硬蜱科中2属2种:硬蜱届的全沟硬蜱和血蜱属的长角血蜱。其中以长角血蜱为优势种,构成比占92.58%(1135/1226);随机对300只长角血蜱进行针对莱姆病螺旋体的PCR.检测,有14只阳性,阳性率为4.67%。并对其余926只蜱进行病原分离培养,未得到莱姆病螺旋体。结论血蜱可能是天津蓟县地区莱姆病传播的主要生物媒介。  相似文献   

4.
承德地区莱姆病自然疫源性特征分析   总被引:1,自引:0,他引:1  
目的了解承德地区莱姆病自然疫源地存在状况。方法于承德林区捕鼠、采蜱;巴伯-斯托纳-凯利培养基(BSK)分离蜱内病原体;PCR扩增蜱、鼠体内目的片段及序列测定;对林区居民及动物牛、羊进行血清抗体检测。结果全沟硬蜱为优势蜱种,从中PCR扩增出目的片段,基因序列同源性比较,提示此感染菌株与欧亚大陆常见的莱姆病螺旋体Borrelia afzelii种具有高度同源性。林区居民及动物牛、羊血清抗体检出阳性率较高,分别为13.3%,23.3%,30%。结论全沟硬蜱是承德地区的优势蜱种;并发现其感染有致病性莱姆病螺旋体Borrelia afzelii种。林区羊、牛为重要的贮存宿主。林区人群存在莱姆病螺旋体自然感染,可初步认定承德地区为莱姆病自然疫源地。  相似文献   

5.
黑龙江省小兴安岭林区莱姆病调查研究   总被引:1,自引:0,他引:1  
目的:探讨小兴安岭林区莱姆病流行情况。方法:间接免疫荧光抗体试验(IFA)、宿主调查、病原分离。结果:林区人群普遍易感,平均感染率1173%。确诊48例莱姆病患者,临床症状以慢性游走性红斑(3333%)、关节炎(2709%)和神经系统损害(2709%)为主。全沟硬蜱是莱姆病螺旋体的主要生物媒介,属野鼠是重要动物宿主。从神经麻痹和游走性红斑病人、全沟硬蜱、棕背、花鼠分离出6株病原体。结论:首次确证该地区为莱姆病自然疫源地  相似文献   

6.
《长寿》1999,(5)
据《健康报》1998年9月15日报道:河北省卫生防疫站最近成功地从日本血蜱中检测出莱姆病螺旋体外膜蛋白(OSPA)基因。研究表明,莱姆病不仅通过全沟硬蜱(花树夹子)传播,日本血蜱也是重要的传播媒介。 莱姆病是经蜱传播的传染病,疫源地是林区和草原。人被带有莱姆病螺旋体的蜱咬伤后即可能发病。局部皮肤出现大片红斑,继而侵犯关节、心脏和神经系统等。 河北省卫生防疫站从1990年开始对境内22个县4170人做了调查,证实人群中存在莱姆病螺旋体特异性抗体,总感染率为0.87%,在林场和草原区高达11.4%和2.6%。为此,本刊特发表余关键的文章以期引起社会关注。  相似文献   

7.
为查明我省是否存在莱姆病的蜱媒及其危害情况,我们于1992年5~9月份对我省承德、保定、邯郸及石家庄地区,采用布旗法在林区草地上和牛、羊等牲畜身上捕捉蜱并进行螺旋体分离培养.按常规法进行,菌株鉴定用直接免疫荧光法和间接免疫荧光法。结果:共采集208只蜱,其中全沟硬蜱147只,占71.01%;森林革蜱35只,占16.91%;长角血蜱25只,占12.08%;日本血蜱仅1只。从101只全沟硬蜱中分离出3株莱姆病螺旋体,命名为HIP6、HIP8及HIP11。  相似文献   

8.
中国20个省、区、市动物莱姆病初步调查研究   总被引:40,自引:6,他引:34  
目的:广泛调查我国动物莱姆病分布情况。方法:血清流行病学和病原学研究。结果:1987~1997年间,牛、羊、狗、野兔和8种野鼠有莱姆病的感染存在,其中牛、羊、狗和野鼠的感染率分别为18.18%~32.61%、17.12%~61.21%、38.5%~60.00%和41.18%~86.05%;从黑线姬鼠和白腹巨鼠的胎鼠分离到莱姆病螺旋体,证实莱姆病螺旋体可通过胎盘垂直传播。9种蜱的中肠携带莱姆病螺旋体,带菌率以全沟硬蜱、二棘血蜱和粒形硬蜱为高,分别为20.00%~45.00%、16.00%~40.00%和24.00%。从华南兔、8种野鼠和8种蜱分离出128株莱姆病螺旋体。证实黑线姬鼠和棕背是中国莱姆病螺旋体的重要储存宿主;多种蜱的中肠内携带莱姆病螺旋体,我国北方林区莱姆病螺旋体的主要生物媒介是全沟硬蜱,南方林区二棘血蜱和粒形硬蜱是重要的生物媒介。结论:我国动物莱姆病广泛存在  相似文献   

9.
大兴安岭南段莱姆病的调查   总被引:1,自引:0,他引:1  
1991年4~6月,我们在大兴安岭南段林区,对莱姆病进行了调查,应用间接免疫荧光抗体法检测496人居民血清,38人血清中抗莱姆病螺旋体IgG抗体效价≥1:128。根据临床表现和血清学调查,确诊莱姆病病例22例;从26只全沟硬蜱分离出4株莱姆病螺旋体。  相似文献   

10.
分离与鉴定莱姆病螺旋体.方法:1996年在山东省费县塔山林场,对莱姆病螺旋体的主要媒介蜱进行调查,用布旗法共采集蜱900只,均为长角血蜱。结果:对50只进行中肠带螺旋体检查,带螺旋体率为12%(6/50)。从86组(850余只)长角血蜱中分离到2株螺旋体(TSH1、TSH3)。该螺旋体对H5332和H9724单克隆抗体呈阳性反应,对H6831单克隆抗体呈阴性反应,证实为莱姆病螺旋体。结论:山东省从长角血蜱中分离到莱姆病螺旋体尚属首次,且首次从病原学上证实山东省林区存在莱姆病疫源地。  相似文献   

11.
三种蜱媒传染病在媒介蜱和鼠类中复合感染的研究   总被引:8,自引:0,他引:8  
目的 了解中国部分地区埃立克体病和其他蜱媒传染病病原体的复合感染情况。方法 运用聚合酶链反应方法对内蒙古自治区、黑龙江省、北京市的蜱和鼠类标本粒细胞埃立克体、莱姆病螺旋体、斑点热群立克次体的感染进行检测,对福建省的蜱和鼠脾脏标本中查菲埃立克体、斑点热立克次体及莱姆病螺旋体的感染情况进行检测。结果 内蒙古采集的全沟硬蜱408只,人粒细胞埃立克体感染率68%,莱姆病螺旋体感染率7.8%,斑点热群立克次体感染率45.6%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本5份(5/408),粒细胞埃立克体与斑点热群立克次体复合感染标本1份(1/408)。黑龙江采集的46只全沟硬蜱中人粒细胞埃立克体感染率6.5%,莱姆病螺旋体感染率10.8%,斑点热群立克次体感染率34.8%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本1份(1/46)。北京市采集的蜱类标本922只,有2只检测到莱姆病螺旋体的DNA片段。福建省宁化地区采集的优势蜱种越原血蜱283组(659只)中,查菲埃立克体最小阳性率为3.8%(25组阳性),38份野鼠脾脏的阳性率为564%(21只阳性),检测出有2只(2/659)蜱同时感染了粒细胞埃立克体和斑点热群立克次体,野鼠的脾脏标本中,也发现有一只同时感染了查菲埃立克体和斑点热立克次体。结论 人粒细胞埃立克体和莱姆病螺旋体复合感染以及查菲埃立克体和斑点热群立克次体复合感染可能在特定地区发生;其地区的蜱类、鼠类存在着极低的复合感染。  相似文献   

12.
Roe deer (Capreolus capreolus) were investigated for their value as sentinel animals for Lyme borreliosis in the Netherlands. Serum was obtained from 114 roe deer, and 513 Ixodes ricinus, predominantly females (72%), were obtained from 47 animals (41%). The polymerase chain reaction was used to detect DNA of Borrelia burgdorferi sensu lato in a total of 190 ticks, comprising 106 engorged ticks and 84 non-engorged ticks. Borrelia DNA was detected in 24 engorged ticks (23%) and 26 non-engorged ticks (31%). This difference was not significant (P = 0.25). Four species of B. burgdorferi sensu lato were identified in the ticks. B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116. B. afzelii was most commonly found and present in 13 mixed infections, and in 28 single infections. Fifteen sera (13%) contained antibodies to Borrelia spp. Ticks are more appropriate sentinel animals for Lyme borreliosis than roe deer, an important host for I. ricinus. Although the viability of borrelia spirochaetes in engorged ticks collected from roe deer was not assessed, a bloodmeal taken from roe deer did not eliminate borrelia spirochaetes from the tick. The relevance of this finding for transovarial transmission of borrelia spirochaetes in ticks is discussed.  相似文献   

13.
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.  相似文献   

14.
Borrelia burgdorferi sensu lato infecting Ixodes persulcatus ticks near Novosibirsk, Russia were detected using PCR with primers specific to 5S and 23S rRNA genes. Two genospecies, B. afzelii and B. garinii, were identified by the PCR-based restriction fragment length polymorphism analysis with Tru9-I restriction endonuclease. Comparison of the corresponding nucleotide sequences revealed considerable diversity of the 5S-23S intergenic spacer structure among B. garinii.  相似文献   

15.
In selected localities of Ceské Bud?jovice and Cesky, Krumlov districts, well known by stable high incidence of tick-borne encephalitis (TBE) human cases but with low incidence of Lyme borreliosis, monitoring of Borrelia burgdorferi sensu lato (s.l.) in Ixodes ricinus ticks was performed. Research was also aimed at the spread of I. ricinus to mountain areas of this region (National Park Sumava), as well as at investigating this tick for B. burgdorferi s.l. genospecies and TBE virus infection. Altogether 498 nymphs, 88 females and 11 males of I. ricinus from lower locations and 58 nymphs from mountain locations (760-1080 m above sea level) were tested by polymerase chain reaction. In lower locations total prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus ticks was 35%. Single infection of Borrelia afzelii, B. garinii and B. burgdorferi sensu stricto (s.s.) was found in 59, 50 and 63 ticks, respectively (i.e. in 12.8, 11.2 and 14.1%). Double infection was found in 42 ticks (6.0%) and triple infection in three ticks (0.4%). The high frequency of B. burgdorferi s.s. exceeds the as yet reported occurrence in Central Europe. These circumstances are discussed. In mountain locations B. afzelii was found in five ticks, that including two co-infection with B. garinii, in elevations of 762 m and 1024 m above sea level, respectively. This fact signals a real danger of human infections in a region that was previously deemed to be without risk. Moreover, this region is more and more the target destination of tourist activities. The results also suggest that the penetration of infection can be rapid and formation and establishment of natural focus of Lyme borreliosis might be rather quick.  相似文献   

16.
我国莱姆病螺旋体生物媒介的初步调查   总被引:4,自引:0,他引:4       下载免费PDF全文
自1987年以来,我们在全国开展了莱姆病调查,从黑龙江、吉林、辽宁、内蒙、河北、新疆、湖北、四川、福建和北京等省、市采集的全沟硬蜱、粒形硬蜱、壤塘硬蜱、嗜群血蜱、二棘血蜱和长角血蜱分离出71株莱姆病螺旋体,其中55株从全沟硬蜱分离。全沟硬蜱是东北和西北针阔叶林区的优势蜱种,其带菌率为20~45%,表明全沟硬蜱是我国北方莱姆病螺旋体的主要生物媒介。虽然从我国南方的某些硬蜱属和血蜱属种类分离到莱姆病螺旋体,但是南方的主要生物媒介还不清楚。从我国蜱体内分离的螺旋体在超微结构、单克隆抗体反应和外表蛋白等方面与美国菌株有某些区别。  相似文献   

17.
目的 了解内蒙古大兴安岭林区蜱和鼠中伯氏疏螺旋体的感染及基因分型情况。方法 应用巢式PCR扩增蜱和鼠中伯氏疏螺旋体5S-23SrRNA间隔区片段,对阳性产物进行限制性片段长度多态性(RFLP)分析和单链构象多态性(SSCP)分析,RFLP分析显示特殊带型的样本测序分析。结果 检测全沟硬蜱1336只,293只阳性,阳性率为21.93%;森林革蜱144只,6只阳性,阳性率为4.17%;嗜群血蜱144只,未发现有伯氏疏螺旋体感染。检测鼠9种145只,感染伯氏疏螺旋体的4种5只,感染率为3.45%;其中检测8只棕背鼠平,2只阳性。RFLP分析及序列分析显示蜱中有B.garinii20047亚型、B.gariniiNT29亚型、B.afzelii基因型以及不同基因型或亚型伯氏疏螺旋体的混合感染;鼠感染的伯氏疏螺旋体包括B.garinii20047亚型和B.gariniiNT29亚型。SSCP分析结果显示带型多于36种。结论 大兴安岭林区蜱及鼠中均存在伯氏疏螺旋体的感染,其中全沟硬蜱的感染率较高;B.garinii型为主要基因型,且该地区伯氏疏螺旋体存在遗传多态性。单只蜱中存在同时感染不同基因型伯氏疏螺旋体的情况,人和宿主动物是否存在不同基因型伯氏疏螺旋体混合感染尚待进一步研究。全沟硬蜱和棕背鼾分别是该林区伯氏疏螺旋体的主要媒介和主要储存宿主。  相似文献   

18.
Questing Ixodes ricinus ticks were collected from six locations throughout Ireland and 638 nymphs, 111 females and 118 males were investigated for infection with Borrelia burgdorferi sensu lato (s.l.). The total prevalence of B. burgdorferi s.l. in the ticks was determined as 14.9% by polymerase chain reaction (PCR) amplification of the spacer region of 5S-23S rRNA genes. Infection prevalence was significantly higher in adult (20.1%) Ixodes ricinus compared to nymphs (13.1%). The prevalence of infection in adult male and female ticks was similar (19.5% and 20.7% respectively). The genomospecies B. burgdorferi sensu stricto, B. afzelii, B. garinii and group VS116 were identified by reverse line blot (RLB) using genomospecies specific oligonucleotide probes. The most prevalent B. burgdorferi genomospecies identified were VS116 (34.6%), B. garinii (24.3%) and B. burgdorferi sensu stricto (18.4%). B. afzelii was uncommon (6.6%). Multiple infections were observed in 13.2% of the infected ticks. The distribution of the genomospecies showed geographical variation and also seemed to be influenced by the nature of the habitat. A broad range of genomospecies seemed to be associated with the presence of a wide spectrum of potential reservoir hosts in the habitat and also with a high overall prevalence of B. burgdorferi s.l.  相似文献   

19.
中国莱姆病螺旋体主要生物媒介的调查研究   总被引:31,自引:3,他引:28  
为研究中国莱姆病螺旋体的生物媒介———蜱的种类、带菌状况及在传播莱姆病中的作用 ,采用布旗法和动物诱捕法在山林地区采集蜱 ,进行分类鉴定 ,应用直接荧光抗体染色法查蜱中肠带菌率 ,BSK培养基分离病原体 ,单克隆抗体鉴定菌株。调查蜱叮咬率与人群莱姆病感染率、患病率以及蜱的季节消长与莱姆病慢性游走性红斑 (ECM )病例发生的季节性关系。在全国 2 0个省 (市、区 )共采集到170 0 0多只蜱 ,分属为 2科 8属 2 3种 ,蜱类分布的地区差异较大 ,北方林区以全沟硬蜱为优势种 ,南方则以二棘血蜱和粒形硬蜱为优势种。 9种蜱的中肠携带莱姆病螺旋体 ,其带菌率以全沟硬蜱、二棘血蜱和粒形硬蜱为高 ,分别为 2 0 .0 0 %~ 4 5.0 0 %、16.0 0 %~ 4 0 .0 0 %和 2 4 .0 0 %。从 8种蜱分离出 10 8株莱姆病螺旋体 ,其单克隆抗体反应与美国B31菌株有明显的差异 ,绝大多数中国菌株对H6831呈阴性反应。蜱叮咬率高的人群 ,莱姆病的感染率和患病率也高。全沟硬蜱的季节消长与ECM发生的季节性相一致。证实多种蜱的中肠内携带莱姆病螺旋体 ;我国北方林区莱姆病螺旋体的主要生物媒介是全沟硬蜱 ,南方林区二棘血蜱和粒形硬蜱是重要的生物媒介。  相似文献   

20.
The diversity and the distribution of tick species and their infection rates by the pathogenic micro-organism Borrelia burgdorferi sensu lato, the etiologic agent of Lyme borreliosis, and Rickettsia sp., were studied in Canton Ticino (the southern part of Switzerland). Ticks specimens collected from animals and humans were classified and analysed for the presence of both pathogens. In particular, PCR analysis was performed for the detection of Borrelia spirochetes in Ixodes ricinus and Ixodes hexagonus, and the hemolymph test was done on Rhipicephalus sanguineus for the detection of Rickettsia sp. PCR assays, performed on 424 of the 989 collected ticks, revealed a low rate of infection (around 2%) of both vectors I. ricinus and I. hexagonus by B. burgdorferi sensu lato. These results are in agreement with the modest number of Lyme borreliosis cases yearly recorded in Ticino. Further, through analysis of DNA sequences, the strains carried by the infected ticks were identified as belonging to the genomic group VS116. The widespread finding of the Mediterranean species Rhipicephalus sanguineus in different locations from July 1994 to October 1995 demonstrates its establishment in Ticino. Of the 210 specimens collected, 70 were analysed and one was infected by Rickettsia sp.  相似文献   

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