胰岛素样生长因子-1及其结合蛋白-3在胃癌诊断中的应用

目的探讨胰岛素样生长因子-1(IGF-1)及其结合蛋白-3(IGFBPs-3)在胃癌诊断中的应用,寻找诊断早期胃癌的标志物,并为胃癌的靶向治疗提供依据。方法采用免疫组化法对58例胃癌患者胃癌组织和正常胃黏膜组织IGF-1及其IGFBPs-3进行检测,并根据临床资料进行相关分析。结果胃癌组织IGF-1阳性率为75.8%,正常组织为27.6%,差异有统计学意义(P0.05)。胃癌组织IGFBPs-3阳性率为70.7%,正常组织为24.1%,差异有统计学意义(P0.05)。胃癌组织中IGF-1和IGFBPs-3的表达呈负相关(r=-0.61,P0.05)。IGF-1与IGFBPs-3表达的阳性率与胃癌患者的年龄、性别无关(P0.05),与分化程度、临床分期及淋巴结转移有关(P0.05)。结论 IGF-1与IGFBPs-3表达的阳性率与胃癌的发生发展有关,可能作为胃癌筛查的标志物之一,为胃癌靶向治疗的研究提供依据。     

血清胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3及骨龄在矮小症儿童诊断中的价值

目的分析血清胰岛素样生长因子-1(IGFs)、胰岛素样生长因子结合蛋白-3(IGFBP-3)及骨龄在矮小症儿童诊断中的价值,以期寻找更为简便、快捷、准确的生长激素缺乏型矮小症的诊断方法。方法选择2014-2016年在生长发育门诊就诊的青春期发育前期患儿80例作为观察组,选择同期行健康体检的健康儿童80例为对照组,所有患儿均符合纳入条件,入组后分别行精氨酸及可乐定联合激发试验判断就诊患儿是否为生长激素缺乏型矮小症,用药前抽取静脉血检测血清IGF-1、IGF-BP3,比较两组患者血清IGF-1、IGF-BP3、骨龄差异。采用中国儿童骨龄行业标准测试骨龄,根据血清IGF-1、IGF-BP3及骨龄测试结果结合激发试验诊断结果,制作血清IGF-1、IGF-BP3、骨龄3种指标及其相应组合诊断矮小症的ROC工作曲线,确定3种指标诊断矮小症的临界限值。以激发试验诊断结果为金标准,计算3种指标及两两组合、三者联合诊断儿童矮小症的特异度、灵敏度和准确度并比较。结果观察组患者血清IGF-1、IGF-BP3及骨龄均明显低于对照组患者(P0.05),血清IGF-1、IGF-BP3、骨龄(骨龄落后生理年龄)的ROC曲线显示其诊断矮小症的临界限值分别为:56μg/L、3 598μg/L、5.4、3.12岁。血清IGF-1、IGF-BP3、骨龄三者结合诊断矮小症的特异度、敏感度高与三指标单纯诊断和两两组合诊断。结论血清IGF-1、IGF-BP3、骨龄三者结合诊断儿童矮小症,简便、快速,因其具有较高的灵敏度,可作为激发试验诊断矮小症的前置诊断,阳性则进一步行激发试验,可有效避免矮小症儿童漏检。     

胰岛素样生长因子-1及其结合蛋白与妊高征

妊娠高血压综合征是常见的严重危害母婴健康的妊娠并发症 ,目前其发病机理尚不明确。近年 ,许多学者提出胎盘或滋养细胞缺血与妊高征发病关系密切。胰岛素样生长因子 1及胰岛素样生长因子结合蛋白 1是生长发育的调节因子 ,被认为与滋养细胞的增殖或侵蚀有关。该文就胰岛素样生长因子 1及胰岛素样生长因子结合蛋白 1的生物学特性、与正常妊娠的关系及其在妊娠高血压综合征发生、发展中的作用进行综述。     

不同胎龄及体重新生儿血Ghrelin、胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3、胰岛素水平检测分析

目的:探讨Ghrelin与新生儿胰岛素-胰岛素样生长因子轴的关系,进一步揭示其对新生儿生长发育及能量代谢的影响。方法:选择62例新生儿,按胎龄、体重分为足月儿、早产儿、适于胎龄儿(appropriate for gestational age,AGA)和小于胎龄儿(small for gestational age,SGA)。其中胎龄30～34周14例,34+1～37周14例,37+1～41周34例。足月SGA13例,足月AGA 21例。检测血Ghrelin、胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)、胰岛素样生长因子结合蛋白-3(insulin-like growth factor binding protein-3,IGFBP-3)以及胰岛素、血糖水平,并在各组间进行比较。结果:30～34周、34+1～37周、37+1～41周3组比较Ghrelin浓度分别为(2.238±0.618)ng/ml(、1.226±0.37)ng/ml(、1.036±0.328)ng/ml,早产儿Ghrelin水平明显高于足月儿(P<0.01),且随着胎龄的增大差距减小;IGF-1分别为(43.214±16.723)ng/ml、(115.579±30.136)ng/ml、(153.292±26.633)ng/ml,IGFBP-3分别为(70.814±22.603)ng/ml、(123.300±28.666)ng/ml、(157.214±38.990)ng/ml,胰岛素分别为(1.032±0.812)μU/ml、(5.534±2.273)μU/ml、(14.654±3.064)μU/ml。IGF-1、IGFBP-3、胰岛素水平早产儿明显低于足月儿(P<0.01),足月SGA的Ghrelin水平明显高于AGA(P<0.01),IGF-1、IGFBP-3、胰岛素水平明显低于AGA(P均<0.01)。各组血糖水平无明显差异(P>0.05)。在SGA和AGA组Ghrelin分别与IGF-1、IGFBP-3及胰岛素呈明显负相关(P<0.01)。结论:SGA新生儿存在胰岛素-IGF轴的损害,IGF-1与胰岛素水平低下,从而使Ghrelin浓度反馈性的升高,以代偿其能量代谢的负平衡。     

重组人生长激素对特发性矮小症患儿胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3的影响

目的探讨重组人生长激素对特发性矮小症患儿胰岛素样生长因子-1 (IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)的影响,为临床治疗提供依据。方法选取2018年1月-2019年6月在该院就诊的特发性矮小症患儿72例为研究对象。采用随机数表法分为观察组和对照组,每组各36例。对照组患儿采取常规治疗,观察组在常规治疗的基础上给予患儿重组人生长激素治疗。比较两组患儿治疗前与连续治疗6个月后胰岛素样生长因子-1 (IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)、生长激素(GH)及碱性磷酸酶(ALP)的变化,记录两组患者治疗前后身高、体质量、身高增长量的差异,统计不良反应发生率。结果治疗后,两组患儿IGF-1、IGFBP-3水平均高于治疗前,且观察组显著高于对照组,差异均有统计学意义(均P<0. 05)。治疗后,两组患儿GH、ALP水平均高于治疗前,且观察组显著高于对照组,差异均有统计学意义(均P<0. 05)。治疗6个月后,两组患儿身高、体质量、身高增长量等基本指标均有所升高,且观察组显著高于对照组,差异均有统计学意义(均P<0. 05)。治疗后,观察组患儿不良反应总发生率显著低于对照组,差异有统计学意义(P<0. 05)。结论重组人生长激素治疗儿童特发性矮小症效果显著,能提高患儿IGF-1、IGFBP-3等血清胰岛素样生长因子水平,有效改善患儿的GH、ALP指标水平,降低不良反应发生情况,促进患儿骨骼发育的同时加快其生长能力,值得临床推广使用。     

胰岛素样生长因子-1及其结合蛋白与妊高征

妊娠高血压综合征是常见的严重危害母婴健康的妊娠并发症，目前其发病机理尚不明确。近年，许多学者提出胎盘或滋养细胞缺血与妊高征发病关系密切。胰岛素样生长因子-1及胰岛素样生长因子结合蛋白-1是生长发育的调节因子，被认为与滋养细胞的增殖或侵蚀有关。该文就胰岛素样生长因子-1及胰岛素样生长因子结合蛋白-1的生物学特性、与正常妊娠的关系及其在妊娠高血压综合征发生、发展中的作用进行综述。     

胰岛素样生长因子结合蛋白-1检测胎膜早破的价值

目的探讨宫颈阴道分泌物中胰岛素样生长因子结合蛋白-1的检测在判断胎膜早破的价值.方法选择89例胎膜早破孕妇和90例正常孕妇,应用免疫层析法对其宫颈分泌物进行胰岛素样生长因子结合蛋白-1检测.结果胰岛素样生长因子结合蛋白-1诊断胎膜早破的敏感性98.89%,特异性91.11%,阳性预测值91.67%,阴性预测值98.93%.结论宫颈阴道分泌物中的胰岛素样生长因子结合蛋白-1可作为诊断胎膜早破的客观指标,对临床有较大应用价值.     

雌二醇 胰岛素样生长因子结合蛋白-3 瘦素在中枢性性早熟女童中的表达及与环境内分泌干扰物的相关性

目的 分析雌二醇(E₂)、胰岛素样生长因子结合蛋白-3(IGFBP3)及瘦素在中枢性性早熟(CPP)女童中的表达及与环境内分泌干扰物的相关性。方法 选取2019年3月—2021年3月台州市立医院收治的88例CPP患儿为观察组,50例健康儿童为对照组。采用化学发光法检测血清E₂水平,采用放射免疫法检测血清IGFBP3、瘦素水平,采用高效液相色谱分析法检测环境内分泌干扰物[邻苯二甲酸二乙酯(DEHP)、辛基酚(OP)及双酚A(BPA)]水平。结果 观察组血清E₂、IGFBP3及瘦素水平分别为(36.21±3.21)pg/ml、(6.47±0.32)mg/L及(6.43±0.52)ng/ml;对照组血清E₂、IGFBP3及瘦素水平分别为(6.23±0.53)pg/ml、(4.25±0.47)mg/L及(2.65±0.22)ng/ml。观察组血清E₂、IGFBP3及瘦素水平均显著高于对照组(t=65.440,P<0.05;t=32.910,P<0.05;t=48.910,P...     

子痫前期患者胎盘中胰岛素样生长因子-Ⅱ和结合蛋白-1的表达及其意义

目的探讨子痫前期患者胎盘组织中胰岛素样生长因子-Ⅱ（IGF-Ⅱ）及胰岛素样生长因子结合蛋白-1（IGFBP-1）的表达特点及其意义。方法采用免疫组织化学的方法检测正常妊娠妇女和子痫前期患者各31例胎盘组织中IGF-Ⅱ及IGFBP-1的表达特点,并进行比较分析。结果两组滋养细胞、血管内皮细胞及绒毛间质细胞均有IGF-Ⅱ、IGFBP-1表达;与正常组相比,子痫前期组IGF-Ⅱ表达降低,差异有统计学意义（P=0．02）;IGFBP-1表达升高,但差异无统计学意义（P=0．26）。结论IGF-Ⅱ、IGFBP-1表达失衡,可能与妊娠期胎盘滋养细胞侵入异常及妊娠期高血压疾病的发病有关。     

使用胰岛素样生长因子结合蛋白-1确诊胎膜早破的临床意义

目的探讨胰岛素样生长因子结合蛋白-1（IGFBP-1）在确诊胎膜早破问题上的临床价值,总结医学经验,从而为临床提供借鉴意义。方法选取克州人民医院2010年12月-2012年12月收治的孕妇90例,根据临床表现,对胎膜情况分为3组,分别为确诊胎膜已破组25例,为1组;胎膜仍然完整组30例,为2组;怀疑胎膜已经破裂组35例,为3组。采用免疫层析法联合羊齿状结晶法对这90例孕妇胎膜早破情况进行诊断,将两种方法检查出来的结果进行比较分析。然后收集患者的资料,对其结果进行回顾性分析。结果 1组：免疫层析法检查结果显示均为阳性,羊齿状结晶法检查结果显示阳性为12例。2组：两种方法检查结果均为阴性。3组：免疫层析法检查结果显示阳性为13例,羊齿状结晶法显示阳性为2例。结论使用IGFBP-1确诊胎膜早破比较准确,操作方便,值得临床进一步推广与应用。     

Dietary soy and fats in relation to serum insulin-like growth factor-1 and insulin-like growth factor-binding protein-3 levels in premenopausal Japanese women

Circulating levels of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-binding protein-3 (IGFBP-3) have each been associated with premenopausal breast cancer risks. We analyzed data from a cross-sectional study of 261 premenopausal Japanese women aged 20-54 yr with adequate nutritional status to evaluate the relationships between concentrations of IGF-1 and IGFBP-3 in serum and dietary intakes of soy, fats and other nutrients. Diet was assessed by a semiquantitative food frequency questionnaire. There was no significant correlation between soy product as well as soy isoflavone intake and serum IGF-1 or IGFBP-3 levels after controlling for age, total energy, percent body fat, and education level. Total fat intake was significantly inversely correlated with serum IGFBP-3 level (r = -0.13, P = 0.04). The correlations of saturated and monounsaturated fats with serum IGFBP-3 were of borderline significance (r = -0.12, P = 0.06 and r = -0.11, P = 0.07, respectively).     

Association between insulin-like growth factor-1, insulin-like growth factor-binding protein-1 and leptin levels with nutritional status in 1-3-year-old children, residing in the central region of Limpopo Province, South Africa

The present study evaluated levels of growth factors and their associations with nutritional status with emphasis on stunting in children at 1 and 3 years of age. A follow-up study on a birth cohort (n 219) of children from villages in the central region of the Limpopo Province was undertaken. Of the original cohort, 156 and 162 could be traced and assessed at ages 1 and 3 years, respectively. Data collected included socio-demographic characteristics, anthropometric measurements, dietary intake and fasting blood (collected from 116 and 145 children at 1 and 3 years, respectively) for growth factor analysis (insulin-like growth factor (IGF)-1, IGF binding protein (BP)-1, IGFBP-3, leptin, glucose and insulin). At 1 year it was found that stunted children had lower leptin levels while their IGFBP-1 levels were higher than that in normal children. These differences were, however, not observed at 3 years. Furthermore at 1 year the biochemical parameters were more related to length measures whereas at 3 years the parameters were more associated with weight measures. The observed stunting in this group of children may be a result of chronic undernutrition resulting in long-term growth faltering which is already evident at 1 year. Thus the observed phenomenon might be an adaptive mechanism adopted by children's metabolic processes as they grow up in an environment with inadequate essential nutrients due to poor weaning practices and consumption of a diet of poor quality, resulting in them gaining more weight at the expense of linear growth.     

Diet, serum insulin-like growth factor-I and IGF-binding protein-3 in European women

OBJECTIVE: The aim of this study was to examine the relationship of diet with serum insulin-like growth factor-I (IGF-I) and IGF-binding protein-3 in women. DESIGN: Cross-sectional study. SETTING AND SUBJECTS: The population are 2109 women who were control subjects in a case-control study of breast cancer nested in the European Prospective Investigation into Cancer and Nutrition. Control subjects were randomly chosen among risk sets consisting of female cohort members alive and free of cancer (except non-melanoma skin cancer) at the time of diagnosis of the index case. Matching criteria were age at enrolment, follow-up time, time of the day of blood collection and study centre. Diet was measured through validated questionnaires. Serum hormone concentrations were measured by enzyme-linked immunosorbent assays. The relationship between serum IGF-I, IGFBP-3, and intake of nutrients and foods was explored by linear regression in models adjusted for energy intake, age, body mass index, smoking, physical activity, centre and laboratory batch. RESULTS: Serum IGF-I levels were positively related to protein intake (P(trend)<0.001), but not related to energy, fat or carbohydrate intake. Positive relationships were observed with the intake of milk (P(trend)=0.007), calcium (P(trend)<0.001), magnesium (P(trend)=0.003), phosphorus (P(trend)<0.001), potassium (P(trend)=0.002), vitamin B6 (P(trend)=0.03), vitamin B2 (P(trend)=0.001) and inverse relationships with vegetables (P(trend)=0.02) and beta-carotene (P(trend)=0.02). IGFBP-3 was not related with most of the nutrients and foods in this study. CONCLUSIONS: In this population, circulating IGF-I is modestly related with the intake of protein and minerals, and with milk and cheese, while IGFBP-3 does not appear to be related with diet.     

先天性甲状腺功能减低患儿胰岛素样生长因子及其结合蛋白的变化

【目的】观察先天性甲状腺功能减低(CH)新生儿及经替代治疗的CH患儿血清中胰岛素样生长因子．1(IGF-1)及胰岛素样生长因子结合蛋白-3(IGFBP-3)的变化。【方法】收集22例CH新生儿、21例替代治疗后的CH患儿静脉血清，采用放射免疫分析法(RIA)测定ICF-1水平，免疫放射分析法(IRMA)测定IGFBP-3水平，并与20例正常新生儿做对照。【结果】与正常新生儿组比较，CH新生儿血清IGF-1和IGFBP-3水平显著降低；替代治疗1～6月后，CH患儿血清IGF-1和IGFBP．3水平较CH新生儿明显升高。各组内IGF-1与IGFBP-3呈正相关，CH新生儿血清IGF-1和IGFBP-3水平与T4、TSH无明显相关性。【结论】CH患儿治疗前血清中IGF-1和IGFBP-3水平明显降低，替代治疗后水平升高。     

血清IGF-1、IGFBP-3水平和大肠癌关系的Meta分析

目的综合评价血清胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)水平和大肠癌的关系。方法利用Meta分析法对6篇关于血清IGF-1、IGFBP-3水平与大肠癌关系的研究文献进行定量综合分析。结果对于IGF-1，合并OR=1．56(95％CI：1．14～2．13)；按实验方法不同分层，间接酶联免疫吸附试验(ELISA)合并OR=1．92(95％CI：1．26～2．93)，IRMA法合并OR=1．23(95％CI：0．78～1．94)；对于IGFBP-3，合并OR=0．78(95％CJ：0．43～1．44)；按实验方法不同分层，ELISA法合并OR=0．46(95％CI：0．29～0．74)，免疫放射测定法(IRMA)合并OR=1．44(95％CI：0．93～2．23)。结论血清IGF-1高水平为大肠癌的独立危险因子，IGFBP-3与大肠癌的关联不具有统计学意义；IGFBP-3与大肠癌关系的各研究之间异质性是由实验方法不同而引起，但该结论尚需大样本并同时进行两种方法的测量证实。     

Hyperinsulinemia,insulin-like growth factor-I,insulin-like growth factor-binding protein-1, and sex-hormone binding-globulin in prepubertal and pubertal girls with premature adrenarche

Precocious adrenarche is defined as the development of pubic hair before the age of 8 years in girls and 9 years in boys. Pubarche caused premature adrenarche in girls has been considered as a normal variant of pubertal development for years. Recently, it is cleared that premature pubarche can be considered as a marker of increased risk for endocrine and metabolic abnormalities. Precocious adrenarche in affected girls is associated with hyperinsulinaemia and functional ovarian hyperandrogenism during puberty. Authors investigated serum levels of insulin-like growth factor-I (IGF-I), insulin-like growth factor-binding protein-1 (IGFBP-1), insulin-like growth factor-binding protein-3 (IGFBP-3), sex hormone binding-globulin (SHBG) and levels of insulin during oral glucose tolerance test in 34 girls with premature adrenarche in 38 age- and BMI-matched healthy controls. Affected girls were assigned into prepubertal and pubertal subgroups. It has been shown that hyperinsulinaemia, decrease in IGFBP-1 and increase IGF-I levels may be present in some affected prepubertal patients. In the pubertal group, in addition to hyprinsulinaemia, decreased IGFBP-1 and increased IGF-I levels an attenuated SHBG level was observed. According to the authors, these laboratory parameters may predict endocrine and metabolic abnormalities in later life. The observed correlations support the hypothesis that insulin/IGF system plays role in the pathogenesis of hyperandrogenism in premature adrenarche and in later hormonal and metabolic changes.     

胰岛素样生长因子及其结合蛋白与妊娠期高血压疾病的相关性研究

目的:研究妊娠期高血压疾病患者血清胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-1(IGFBP-1)的水平与病情程度及新生儿出生体重之间的关系。方法:采用放射免疫方法测定并比较38例妊娠期高血压疾病患者与38例正常血压妊娠妇女的血清IGF-1、IGFBP-1的水平。结果:子痫前期组IGF-1显著低于妊娠期高血压组和正常组,而IGFBP-1水平显著高于妊娠期高血压组和正常组;妊娠期高血压组与正常组间IGF-1、IGFBP-1的水平比较,差异均无统计学意义。IGF-1水平与收缩压、舒张压及平均动脉压呈显著负相关,与新生儿出生体重呈显著正相关,而IGFBP-1与收缩压、舒张压及平均动脉压呈显著正相关,与新生儿出生体重呈显著负相关。结论:妊娠期高血压疾病患者的发病及严重程度与IGF-1、IGFBP-1有明显的关系,IGF-1、IGFBP-1与胎儿的发育及新生儿出生体重有明显的相关性。     

Effect of lycopene supplementation on insulin-like growth factor-1 and insulin-like growth factor binding protein-3: a double-blind, placebo-controlled trial

OBJECTIVE: Studies have suggested a link between lycopene and insulin-like growth factor-1 (IGF-1). The aim of this study was to test the effect of lycopene supplementation on IGF-1 and binding protein-3 (IGFBP-3) status in healthy male volunteers. DESIGN, SETTING, SUBJECTS AND INTERVENTION: This was a 4 week randomized, double-blind, placebo-controlled study of lycopene supplementation (15 mg/day) in healthy male volunteers (n=20). Fasting blood samples were collected at baseline and after 4 weeks. Samples were analysed for lycopene by high-performance liquid chromatography (HPLC) and IGF-1 and IGFBP-3 by enzyme-linked immunosorbent assay (ELISA). Changes in end points from baseline were compared in those who received placebo versus those who received the lycopene supplement. RESULTS: Median change in lycopene from baseline (post-supplement - baseline) was higher in subjects in the intervention than those on placebo (lycopene group 0.29 (0.09, 0.46); placebo group 0.03 (-0.11, 0.08) micromol/l; median (25th, 75th percentiles), P<0.01). There was no difference in median change in IGF-1 concentrations (lycopene group -0.6 (-2.6, 1.9); placebo group -1.15 (-2.88, 0.95) nmol/l, P=0.52), or median change in IGFBP-3 concentrations (lycopene group 245 (-109, 484); placebo group 101 (-34, 234) nmol/l, P=0.55) between intervention and control groups. Change in lycopene concentration was associated with the change in IGFBP-3 in the intervention group (r=0.78; P=0.008; n=10). CONCLUSIONS: Lycopene supplementation in healthy male subjects has no effect on IGF-1 or IGFBP-3 concentrations in a healthy male population. However, the association between change in lycopene concentration and change in IGFBP-3 in the intervention group suggests a potential effect of lycopene supplementation on IGFBP-3.     

Insulin-like growth factor 1, insulin-like growth factor-binding protein 3, and testicular germ-cell tumor risk

Studies have consistently shown that taller men are at increased risk of testicular germ-cell tumors. Thus, it is plausible that factors associated with height may also influence risk of these tumors. The authors examined associations between testicular germ-cell tumor risk and circulating concentrations of insulin-like growth factor 1 (IGF-1) and insulin-like growth factor-binding protein 3 (IGFBP-3) among 517 cases and 790 controls from the US Servicemen's Testicular Tumor Environmental and Endocrine Determinants (STEED) Study (2002-2005). Odds ratios and 95% confidence intervals were estimated using logistic regression models, adjusting for age, race/ethnicity, height, and body mass index. All tests of significance were two-sided. Overall, there were no associations between IGF-1 or IGFBP-3 concentrations and risk of testicular germ-cell tumors (p > 0.05). However, when cases were separated by histologic type, there was a suggestion of a reduction in seminoma risk associated with the highest concentrations of IGF-1 as compared with the lowest concentrations (odds ratio = 0.66, 95% confidence interval: 0.40, 1.09). Although there were no overall associations with insulin-like growth factor, contrary to expectation, there was a suggestion that IGF-1 concentrations may be inversely associated with risk of seminoma.     

原发性肝癌患者血清IGFBP-3浓度的测定及临床意义

目的探讨原发性肝癌患者血清胰岛素样生长因子结合蛋白-3(IGFBP-3)浓度的变化及对其诊断和发病机制的意义。方法用酶联免疫法(ELISA)检测了25例原发性肝癌、10例正常对照、21例慢性肝炎(中度10例、重度11例)及11例肝硬化患者血清IGFBP-3的浓度。用单因素方差分析比较各组IGFBP-3的浓度,用成组设计的两样本均数比较的t检验比较肝癌患者组内区别。结果正常对照、慢性肝炎、肝硬化及肝癌患者血清IGFBP-3的浓度分别为(4891.63±1482.91)、(3192.67±779.86)、(1146.93±443.15)及(1760.61±958.88)ng/m l。肝癌患者中AFP阳性组与阴性组之间差异无统计学意义。无肝硬化基础的肝癌患者血清IGFBP-3浓度稍高于在肝硬化基础上发生的肝癌患者,但二者之间以及与肝硬化患者之间差异无统计学意义。结论血清IGFBP-3浓度的显著降低对肝癌的诊断有辅助意义,尤其是无慢性肝炎及肝硬化的患者。