鼻咽癌组织中bcl—2及EB病毒潜伏膜蛋白表达与放射诱发细胞？…

目的：探讨鼻咽癌（ＮＰＣ）组织ｂｃｌ－２及ＥＢ病毒潜伏膜蛋白（ＬＭＰ）表达与放射诱发细胞凋亡的关系。方法采用免疫组化Ｓ－Ｐ法及ＴｄＴ酶介导的生物素化ｄＵＴＰ缺口要端标记技术（ＴＵＮＥＬ法）,分别检测３５例ＮＰＣ组中ｂｃｌ－２及ＥＢ病毒ＬＭＰ的表达,以及放疗总量为１０Ｇｙ时ＮＰＣ组织的细胞凋亡率（ＡＲ）。结果ＮＰＣ组织ｂｃｌ－２及ＬＭＰ的表达率分别为７１．４％（２５／３５）、４５．７％（１６／３５）     

鼻咽癌组织中EB病毒LMP1的表达与癌细胞增殖和凋亡的关系

目的：探讨ＥＢ病毒编码的ＬＭＰ１在体内鼻咽癌细胞中,是否可能通过影响鼻咽癌细胞增殖和凋亡的生物学过程,而在鼻咽癌的发展演进过程中起作用。方法：采用ＴＵＮＥＬ法原位检测癌细胞的凋亡程度,用免疫组化ＬＳＡＢ法检测ＬＭＰ１、ＰＣＮＡ、Ｐ５３和ｂｌｃ－２蛋白表达;分析ＬＭＰ１的表达与癌细胞增殖、凋亡以及与ｐ５３、ｂｃｌ－２表达的相关性。结果：ＬＭＰ１的表达与否,与鼻咽癌细胞增殖活性的差异无显著意义,而与癌     

绿茶儿素对于人肝癌细胞的凋亡诱导作用

目的：探讨绿茶儿茶素（ＣＴＣ）对ＢＥＬ－７４０２人肝癌细胞的凋亡诱导作用。方法：应用透射电镜、流式细胞术、ＴＵＮＥＬ和免疫组化等方法检测受１００或２００ｕｇ／ｍｌＧＴＣ作用４天后的ＢＥＬ－７４０２细胞的凋亡情况及其ＰＣＮＡ、ｂｃｌ－２、ｃ－ｍｙｃ、ｐ５３蛋白表达情况。结果：受ＧＴＣ作用４天后的ＢＥＬ－７４０２细胞的凋亡显著性增加,其ＰＣＮＡ表达显著性下降,而与凋亡相关的癌基因ｂｃｌ－２、ｃ－ｍｙｃ     

中国南方鼻咽鳞状细胞癌与EB病毒感染的关系

目的：探讨中国南方鼻咽癌高发区鼻咽鳞状细胞癌或称角化性鳞状细胞癌（Ｋｅｒａｔｉｎｉｚｉｎｇ ｓｑｕａｍｏｕｓ ｃｅｌｌ ｃａｒｃｉｎｏｍａ,ＫＳＣＣ）是否也如非角化性癌那样与ＥＢ病毒感染有密切的关系。方法：应用ＰＣＲ Ｓｏｕｔｈｅｒｎ ｂｌｏｔｔｉｎｇ、原 位分子杂交和免疫组化法检测３８例鼻咽鳞状细胞癌细胞中有ＥＢ病毒ＤＮＡ及其产物ＥＢＮＡ－１,ＥＢＮＡ－２,ＥＢＥＲＳ,ＬＭＰ－１,ＺＥＢＲＡ,Ｅ     

鼻咽癌中p53蛋白积聚对瘤细胞有丝分裂和凋亡的影响

目的：观察疗前鼻咽癌组织中Ｐ５３蛋白的积聚及其对瘤细胞有丝分裂和凋亡的影响。方法随机收集１９９７年疗前鼻咽癌活检标本４３例,采用免疫组化ＬＳＡＢ法ＤＯ－７一抗检测Ｐ５３蛋白的表达。在Ｈ＆Ｅ染色切片上位细胞死亡试剂盒检测瘤细胞凋亡,平均每个高倍视野下的凋亡瘤细胞数为凋亡指数（ＴＵＮＥＬ ｉｎｄｅｘ,Ｔ１）。比较高于位细胞死亡试剂盒检测瘤细胞凋亡,平均每个高倍视野下的细胞瘤细胞数为凋亡指数（ＴＵＮＥＬ     

细胞凋亡与子宫颈癌的发生

目的 探讨细胞凋亡与子宫颈癌发生、发展的关系。方法 １９０例手术切除标本中,正常宫颈鳞状上皮（ＮＥ）４１例;上皮内瘤样病变７８例,其中重度非典型增生（ＳＤ）４１例,原位癌（ＣＩＳ）３７例;早期侵袭癌（ＭＩＣ）３１例;大细胞非角化型浸润癌（ＩＣ）４０例。凋亡细胞检出用ＴＤＴ－ｍｅｄｉａｔｅｄ ｄＵＴＰｂｉｏｔｉｎ ｎｉｃｋ ｅｎｄ ｌａｂｅｌｉｎｇ（ＴＵＮＥＬ）方法,增生细胞核抗原（ＰＣＮＡ）、ｐ５３、ｂｃｌ－２基因蛋白表达采用免疫组化ＡＢＣ染色方法。结果 （１）在ＮＦ组,凋亡细胞分布在表层细胞,增生细胞却局限于深层细胞;在宫颈肿瘤性病变组织中,二者均毫无规律地散在于病灶中。（２）ＴＵＮＥＬ标识率伴随病变进展而降低,尤其从ＮＥ到ＣＩＳ组显著地减少（Ｐ〈０．０１）,而ＰＣＮＡ标识率却伴随病变进展呈进行性增加（Ｐ〈０     

细胞凋亡及其相关基因与乳腺癌预后关系的研究

目的：分析乳腺癌中凋亡细胞数量、凋亡相关基因－－ｂｃｌ－２、ｐ５３的表达、细胞增殖标志物ＰＣＮＡ（增殖细胞核抗原）的表达,与影响乳腺癌预后的因素（肿瘤大小,ＴＮＭ分期,淋巴结转移情况,雌孕激素受体情况等）之间的关系及其对预后的影响。方法：凋亡细胞的检测采用原位ＤＮＡ缺口末端标记ＴＵＮＥＬ法,ｂｃｌ－２、ｐ５３、ＰＣＮＡ、ＥＲ（雌激素受体）及ＰＲ（孕激素受体）检测采用免疫组化ＬＳＡＢ法。结果：本组２     

Fas,Bax表达及细胞凋亡与胃癌浸润及转移的关系

目的 探讨Ｆａｓ、Ｂａｘ蛋白在胃腺癌中的表达及其与细胞凋亡的关系。方法 采用免疫组织化学ＡＢＣ法及细胞凋亡ＴＵＮＥＬ法检测４８例胃腺癌中Ｆａｘ、Ｂａｘ蛋白的表达及细胞凋亡。结果 Ｆａｓ、Ｂａｘ蛋白在高、中分化胃癌中的阳性表达率显著高于低分化胃癌（Ｐ〈０．０５）;Ｆａｓ、Ｂａｘ蛋白在有淋巴结转移癌中的阳性表达率均为２３．８％,显著低于无淋巴结转移癌的５５．８％和５１．９％（Ｐ〈０．０５）,Ｆａｓ蛋白     

子宫颈鳞状细胞癌细胞外间质改变的免疫荧光抗体法研究

目的：通过对细胞外间质（ＥＣＭ）成分进行检测,探讨间质重构与子宫颈鳞状细胞癌侵袭性生长及播散的关系。方法：应用双重免疫荧光染色技术和激光共聚焦显微镜观察,对正常人及不同分化程度的子宫颈鳞状细胞癌患者的子宫颈石蜡包埋组织切片纤维结合素（ＦＮ）、层粘连蛋白（ＬＮ）及Ⅰ、Ⅲ、Ⅳ型胶原蛋白的表达进行检测。结果：正常子宫颈组织中,ＥＣＭ内可见结构致密的Ⅰ、Ⅲ型胶原和细条索状的ＦＮ蛋白表达,Ⅳ型胶原和ＬＮ蛋白     

EB病毒LMP1促鼻咽癌细胞生长与CD23、bcl-2表达和凋亡的关系

目的：探讨ＥＢ病毒ＬＭＰＩ表达促鼻咽细胞系生长作用与ＣＤ２３、ｂｃｌ－２表达和细胞凋亡的关系。方法：用免疫组化ＬＳＡＢ法检测ＬＭＰＩ、ＣＤ２３和ｂｃｌ－２蛋白的表达;用ＭＴＴ法测定鼻咽癌细胞系的生长能力;用ＤＮＡＪ电泳法、流式细胞法和ＴＵＮＥＬ法检测癌细胞凋亡;用ＣＤ２３单抗阻断和ＭＴＴ法观察ＣＤ２３单抗对鼻咽产纱生长的影响。结果：表达ＬＭＰＩ的鼻咽癌细胞系（Ｌ－ＣＮＥＩ）的生长能力明显增强,并有     

食管癌survivin、caspase-3表达与细胞凋亡的关系

目的探讨survivinmRNA、caspase-3蛋白在食管鳞癌中的表达及其与细胞凋亡的关系。方法分别采用RT-PCR和免疫组化技术检测38例食管鳞癌及其对应的正常组织中survivinmRNA和caspase-3蛋白的表达,运用TUNEL方法检测细胞凋亡。结果73.68%食管鳞癌组织呈survivinmRNA阳性表达,显著高于正常组织,并与食管鳞癌的分化程度、临床分期有关,与年龄、性别、淋巴结转移无关;caspase-3蛋白在食管鳞癌和正常组织中的阳性表达率分别为26.32%和89.47%,差异有显著性,并与食管鳞癌的分化程度有关,与年龄、性别、淋巴结转移和临床分期无关;在食管鳞癌组织中,survivinmRNA和Caspase-3蛋白表达呈负相关;survivinmRNA阳性的食管鳞癌组中细胞凋亡指数明显低于survivinmRNA阴性组,(P<0.01),caspase-3蛋白阳性的食管鳞癌组中平均细胞凋亡指数明显高于caspase-3蛋白阴性组(P<0.01)。结论survivin、caspase-3参与了食管鳞癌的发生、发展,可以作为食管鳞癌预后的指标,survivin通过抑制caspase-3的活性而发挥其抑制细胞凋亡的作用是其主要的分子机制。     

The effect of fatty acid-CoA ligase 4 on the growth of hepatic cancer cells

In this study, we detected the expression of FACL4 mRNA in 40 patients with hepatic carcinoma and its adjacent normal tissues by semi-quantitative RT-PCR. The changes of proliferation and apoptosis of hepatic cancer cell line HepG2 with FACL4 protein expression were examined by MTT and flow cytometry respectively after FACL4 selective inhibitor triacsin C treatment. The activity related to apoptosis of proteinases, caspase-3, caspase-8 and caspase-9, were detected by colorimetry. The expression related to apoptosis of protein, wt-p53, Bax and Bcl-2, in HepG2 cells were evaluated by S-P immunocytochemical dyeing. The results were: (1) FACL4 mRNA was expressed in 95.0% of hepatic cancer tissue, while the positive expression of FACL4 mRNA was 82.5% in cancer adjacent normal liver tissues. Moreover, there was a statistically significant increased in quantity of FACL4 mRNA in cancer tissues compared with adjacent normal liver tissues. (2) The concentration of triacsin C (0.5-2 mg/L) could inhibit the proliferation and induce the apoptosis of HepG2 cells significantly in a dose- and time-effect. (3) During the apoptosis of HepG2 cells induced by triacsin C, flow cytometry coupled with Rhodamine 123 dyeing showed that mitochondrial transmembrane potential of HepG2 declined significantly, and the activity of caspase-9 and caspase-3 increased more remarkably than caspase-8. Besides, the increased apoptosis was accompanied by increased Bax, and decreased wtp53 and Bcl-2 protein levels. The present study suggested that FACL4 might play a role in the growth of hepatic cancer cells. FACL4 selective inhibitor triacsin C leads to a marked growth inhibition of human liver tumor cells, based on the inhibition of proliferation and induction of apoptosis. The apoptotic process was mediated by intrinsic mitochondrial apoptotic pathway due to activation of caspase-9 and caspase-3. The increased apoptosis was accompanied by upregulation of Bax, and decreased wt-p53 and Bcl-2 protein level.     

人脐带血间充质干细胞对人舌鳞癌移植瘤生长的影响及作用机制

目的:探讨人脐带血间充质干细胞（HUC-MSCs）对人舌鳞癌细胞（Cal-27）移植瘤生长的影响及其相关特异性蛋白表达机制。方法:将Cal-27细胞移植到BALB/c裸小鼠皮下,待裸鼠肿瘤体积达到50 mm3成瘤标准后通过裸鼠尾静脉注射经GFP绿色荧光蛋白标记的HUC-MSCs,然后研究HUC-MSCs对预先建立的Cal-27细胞移植瘤生长状况的影响。为了阐明其发生机制,我们对具体阳性实验组及阳性对照组肿瘤组织的细胞增殖及凋亡相关蛋白Bcl-2、Bax、c-Myc、β-catenin的相对mRNA表达量进行了q-PCR分析实验。结果:在预先建立人舌鳞癌的体内模型中,通过尾静脉注射的经GFP绿色荧光蛋白标记的HUC-MSCs可以在肿瘤组织内富集并可抑制肿瘤生长。通过实时荧光定量PCR实验,我们发现阳性实验组相较于阳性对照组肿瘤组织细胞内Bax的mRNA表达量上调,Bcl-2、β-catenin、c-Myc的mRNA表达量下调。结论: HUC-MSCs通过促进Bax/Bcl-2形成同源二聚体以及抑制Wnt/β-catenin信号通路可抑制Cal-27细胞移植瘤生长。     

Paradoxically abundant expression of Bcl-2 and adrenomedullin in invasive cervical squamous carcinoma

Background: The purpose of this study was to identify the role of Bcl-2 protein and adrenomedullin (AM) expression in relation to apoptosis in the neoplastic changes of squamous epithelium of the uterine cervix. Methods: Apoptosis in sections of normal cervical epithelium, cervical intraepithelial neoplasia (CIN), and invasive squamous cell carcinoma was determined by terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate nick-end labeling (TUNEL) assay. Bcl-2 protein and AM expression were analyzed using the avidin-biotin immunoperoxidase method. Results: On the basis of the TUNEL assay, the apoptosis positive rate of the nuclei in early invasive squamous carcinoma and in bulky invasive squamous carcinoma was significantly higher than that in normal cervical epithelium, but no such increase in the apoptosis-positive rate was noted in the nuclei in CIN. Immunohistochemical staining revealed that Bcl-2 protein and AM were immunolocalized in the cytoplasm of invasive squamous carcinoma cells, but not in either CIN lesions or normal cervical epithelium. Bcl-2 protein expression was more prominent in bulky invasive squamous carcinoma cells than in early invasive squamous carcinoma cells. AM expression was also more abundant in bulky invasive squamous carcinoma compared with that in early invasive squamous carcinoma. Conclusion: The present results suggest that the expression of Bcl-2 protein and AM in invasive squamous carcinoma may play crucial roles in selecting carcinoma cells resistant to apoptosis and in promoting malignant progression. Received: May 15, 2002 / Accepted: January 27, 2003 Acknowledgments This work was supported in part by a Grant-in-Aid for Scientific Research (no. 10470346) from the Japanese Ministry of Education, Science, and Culture. Correspondence to:T. Maruo     

Analysis of thermochemotherapy-induced apoptosis and the protein expressions of Bcl-2 and Bax in maxillofacial squamous cell carcinomas

The aim of this study is to investigate the relationship between thermochemotherapy-induced apoptosis and the expressions of the Bcl-2 and Bax proteins in maxillofacial squamous cell carcinomas. Fifteen patients with maxillofacial squamous cell carcinomas were treated with microwave hyperthermia (43 °C for 40 min) following the intravenous injection of pingyangmycin (Bleomycin A5 Hydrochloride for Injection) (8 mg). Subsequently, the tumors were surgically resected. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end labeling method was used to assess the apoptosis in the carcinoma cells, and immunohistochemistry was performed using the streptavidin-peroxidase method to determine the expression levels of the Bcl-2 and Bax proteins, and quantitative analysis was employed. The number of apoptotic cells increased markedly (P < 0.001). The protein expression of Bcl-2 was downregulated (P < 0.001), while that of Bax was markedly upregulated (P < 0.001). Thermochemotherapy induces apoptosis in maxillofacial squamous cell carcinoma cells by downregulating the protein expression of Bcl-2 and upregulating that of Bax.     

EBV-miR-BART5-3p靶向p53对鼻咽癌细胞放射敏感性的影响

目的:研究爱泼斯坦-巴尔病毒（EBV）-miR-BART5-3p对鼻咽癌细胞放射敏感性的影响,并探讨其作用机制。方法:体外培养人EBV阳性鼻咽癌细胞（C666-1）和人鼻咽癌细胞（CNE-2Z）,将CNE-2Z组细胞设置为正常组,C666-1细胞随机分为对照组、EBV-miR-BART5-3p NC组、EBV-miR-BART5-3p mimics组和EBV-miR-BART5-3p inhibitor组。用实时荧光定量PCR（RT-qPCR）法检测各组细胞及EBV阴性鼻咽癌患者和EBV阳性鼻咽癌患者癌组织中EBV-miR-BART5-3p表达情况,噻唑蓝（MTT）法检测各组细胞存活率,平板克隆实验评估各组放疗敏感性变化情况,膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）法检测各组细胞凋亡敏感性变化,蛋白免疫印迹分析法检测转染后各组细胞p53、Bcl-2相关X蛋白（Bax）、半胱天冬氨酸酶-3（caspase-3）和Bcl-2蛋白表达情况,双荧光素酶报告实验验证EBV-miR-BART5-3p与p53的靶向关系。结果:与EBV阴性组相比,EBV阳性组鼻咽癌组织中EBV-miR-BART5-3p表达水平显著升高（P＜0.05）。与正常组相比,对照组EBV-miR-BART5-3p表达、存活率、克隆数量和Bcl-2蛋白表达水平显著升高（P＜0.05）,凋亡率、p53、Bax和caspase-3蛋白表达水平显著降低（P＜0.05）。与对照组和EBV-miR-BART5-3p NC组相比,EBV-miR-BART5-3p mimics组EBV-miR-BART5-3p表达水平、存活率、克隆数量和Bcl-2蛋白表达水平显著升高（P＜0.05）,凋亡率、p53、Bax和caspase-3蛋白表达水平显著降低（P＜0.05）;与对照组和EBV-miR-BART5-3p NC组相比,EBV-miR-BART5-3p inhibitor组EBV-miR-BART5-3p表达水平、存活率、克隆数量和Bcl-2蛋白表达水平显著降低（P＜0.05）,凋亡率、p53、Bax和caspase-3蛋白表达水平显著升高（P＜0.05）。双荧光素酶报告实验结果显示,与TP53-3' UTR-WT+EBV-miR-BART5-3p NC组比较,TP53-3' UTR-WT+EBV-miR-BART5-3p inhibitor组荧光素酶活性降低（P＜0.05）。结论:下调EBV-miR-BART5-3p可能通过靶向促进p53蛋白表达,提高人EBV阳性鼻咽癌细胞的放射敏感性。     

Apoptosis and the expression of Bax and Bcl-2 in squamous cell carcinoma and adenocarcinoma of the uterine cervix

BACKGROUND: Apoptosis plays a crucial role in the suicide and turnover of cells in various tumors. This study was designed to investigate the relation between apoptosis and the histologic types of cell in invasive cervical carcinoma. METHODS: Cervical tissues were obtained from 19 patients with invasive squamous cell carcinoma (ISCC), 9 patients with invasive endocervical adenocarcinoma (IEAC), and 15 patients with myoma uteri (which were used as controls). Each tissue was rapidly frozen and/or fixed in Bouin's solution. The occurrence of apoptosis was examined by end labeling of DNA with (alpha-32P)dideoxyATP and electrophoretic fractionation and by end labeling of DNA in situ with digoxigenin-dideoxyUTP. The expression of Bcl-2 and Bax proteins was examined by immunohistochemical staining with appropriate antibodies. RESULTS: Autoradiographic analysis revealed that high molecular weight DNA was predominant in the normal cervical epithelium (NCE) and in ISCC. However, a ladder-like pattern of DNA fragments, characteristic of the apoptotic breakdown of DNA, was identified in IEAC. Quantitative analysis of low molecular weight fragments of DNA revealed a significant increase in IEAC but not in ISCC compared with NCE. Labeling of DNA in situ indicated that cells undergoing apoptosis were predominant among the neoplastic cells of IEAC. However, no apoptotic cells were noted in ISCC, with the exception of cells in some tumor nests. A large fraction of IEAC and ISCC was immunonegative for Bcl-2. Although the expression of Bax was detected weakly in a small fraction of ISCC, strong expression of Bax was observed in all cases of IEAC. CONCLUSIONS: Apoptosis appears to occur in the cancerous cells of invasive adenocarcinoma of the uterine cervix in association with a high level of expression of Bax but not of bcl-2.     

Chemosensitivity and p53-Bax pathway-mediated apoptosis in patients with uterine cervical cancer.

OBJECTIVES: To determine whether and how apoptosis through the p53-Bax pathway affects sensitivity to chemotherapy in cervical cancer. MATERIALS AND METHODS: Thirty patients with cervical squamous cell carcinoma, who had human papilloma virus (HPV) and underwent neoadjuvant chemotherapy, were entered in the present study. Tumor specimens were obtained before and after chemotherapy. HPV was detected by polymerase chain reaction. The expression of Ki-67, p53, Bax and Bcl-2 proteins was determined by immunohistochemical staining. Apoptotic cells were identified by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling method. RESULTS: Of 30 patients, 18 responded to chemotherapy and 12 did not. The apoptotic index in tumors of responders was significantly higher than in non-responders after chemotherapy. The Ki-67 labeling index (LI) in responders was significantly higher than in non-responders before chemotherapy. Patients with tumors >33% of the LI, which was determined by a receiver operating characteristic curve, had a better survival rate. The incidence of p53 protein expression did not differ between responders and non-responders. After chemotherapy, the expression of Bax protein in responders was more frequent and Bcl-2 protein expression was less frequent than in non-responders. CONCLUSIONS: Chemosensitivity in cervical cancer may be associated with apoptosis via the p53-Bax pathway.     

线粒体凋亡途径在TA2小鼠自发性乳腺癌中的作用

比较TA2小鼠正常乳腺、乳腺癌前病变和乳腺癌组织中细胞增殖和凋亡的情况,通过检测线粒体凋亡途径中Bcl-2、Bax、Caspase-3和Caspase-9在乳腺组织中的表达,初步确定线粒体凋亡途径在TA2小鼠自发乳腺癌中可能发挥的作用。方法:收集正常TA2成年雌鼠的乳腺组织（NC组）和TA2自发性乳腺癌癌前病变组织（SBC-b组）和乳腺癌组织（SBC-t组）,采用免疫组化方法检测各组乳腺上皮细胞中PCNA、Bcl-2、Bax、Caspase-3和Caspase-9的表达并计算增殖指数（PI）;采用TUNEL方法检测细胞凋亡并计算凋亡指数（AI）;采用Real-time PCR和Western blot方法检测组织中Bcl-2、Bax、Caspase-3和Caspase-9蛋白表达及mRNA相对表达水平。结果:免疫组化染色,Real-time PCR以及Western blot结果一致显示,SBC-b组Bcl-2,Bax,Caspase-3和Caspase-9表达均明显高于NC（P<0.01或P<0.05）;SBC-t组中除Bcl-2高于NC外,其余蛋白表达均明显低于NC（P<0.01）,但bcl-2,bax和Caspase-3 mRNA表达均显著高于NC（P<0.01）,Caspase-9 mRNA略高于NC,但无统计学意义（P>0.05）。结论:线粒体途径可能参与了TA2鼠自发性乳腺癌的发生,其通过刺激部分乳腺上皮细胞凋亡,破坏了TA2小鼠乳腺上皮细胞增殖和凋亡的平衡,以致乳腺癌发生。      

姜黄素对子宫颈癌HeLa细胞增殖抑制作用及其机制的研究

目的:探讨姜黄素(Curcum in)对体外培养人子宫颈癌HeLa细胞抗癌作用及分子机制。方法:MTT法检测细胞增殖,流式细胞仪检测凋亡和细胞周期,PI/Hoechst33258荧光双染法检测细胞凋亡,W estern b lot法检测细胞凋亡相关蛋白Bc l-2和Bax蛋白的表达。结果:姜黄素对HeLa细胞生长有抑制作用,并呈剂量依赖性;流式细胞仪分析证实姜黄素能使HeLa细胞阻滞在S期,并出现亚二倍体凋亡峰;荧光双染法可见凋亡细胞;W estern b lot结果显示Bax蛋白表达均上调,而Bc l-2的表达无明显影响。结论:姜黄素对人子宫颈癌HeLa细胞的增殖具有显著的抑制作用并可诱导细胞凋亡;Bax蛋白的表达上调可能参与了诱导细胞凋亡。