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1.
目的:SK3是小电导型钙依赖钾通道之一,是内皮依赖性超极化因子(endothelium-derived hyperpolar-izing factor,EDHF)通路中关键物质,本研究探讨糖尿病对大鼠阴茎海绵体小电导钙激活性钾通道蛋白SK3表达的影响。方法:雄性SD大鼠60只,其中50只大鼠采用腹腔注射链脲佐菌素(streptozocin,STZ)制作糖尿病模型组(DM),注射STZ未能成模大鼠用作STZ药物对照组(STZ),其余10只大鼠用于空白对照组。造模成功后饲养8周,注射阿朴吗啡(apomorphine,APO)80μg/kg后,观察大鼠阴茎勃起情况;随后采用RT-PCR、Western印迹技术检测SK3mRNA和蛋白在大鼠阴茎海绵体中的表达水平。结果:DM组(26只)有14只大鼠阴茎勃起,勃起率为54%,STZ组(15只)和空白对照组(10只)勃起率均为100%。DM组SK3mRNA表达(0.50±0.09)显著低于STZ组(1.15±0.03)和空白对照组(1.21±0.04)(P<0.05)。DM组SK3蛋白表达(0.65±0.06)与STZ组(1.28±0.04)和空白对照组(1.34±0.05)相比存在显著差异(P<0.05)。STZ组和空白对照组之间大鼠阴茎勃起情况和SK3mRNA及蛋白表达无差异(P>0.05)。结论:糖尿病可明显降低大鼠阴茎勃起功能,且这可能与大鼠阴茎海绵体SK3表达减少密切相关。  相似文献   

2.
目的:探讨L-肉碱(LC)对糖尿病(DM)大鼠生精细胞凋亡及附睾精子数量和活动率的影响。方法:24只雄性SD大鼠随机均分为3组,一组作为对照组,剩余两组分别注射链脲佐菌素(STZ,65 mg/kg)建立DM模型。建模成功后,各组大鼠分别给予如下灌胃剂量:对照组:生理盐水;DM模型组:生理盐水;LC组:300 mg/kgLC溶液,连续灌胃6周。末次给药24 h后,麻醉处死所有大鼠,分别进行附睾精子计数并检测精子活动率,流式细胞术检测各组大鼠睾丸生精细胞凋亡情况。结果:用LC治疗后的大鼠附睾头、尾精子活动率(%)分别为53.7±1.8和60.3±1.6,显著高于DM模型大鼠(分别为32.2±2.0和40.5±1.4,P<0.05),但低于对照组大鼠精子活动率63.1±2.4和68.9±1.3。与对照组附睾尾精子相对计数[(37.8±1.1)×106/100 mg]相比,DM组显著减少[(25.5±1.1)×106/100 mg],且具有统计学差异(P<0.05);LC治疗后大鼠附睾尾精子相对计数[(32.0±1.5)×106/100 mg]比DM组显著增加(P<0.05),但仍低于对照组。与对照组生精细胞凋亡率[(3.7±1.3)%]相比,DM组生精细胞凋亡率[(52.5±4.4)%]显著上升(P<0.05);经LC治疗后,LC组大鼠生精细胞凋亡率为(35.3±3.5)%,比DM组显著降低(P<0.05),但仍显著高于对照组。结论:LC(300 mg/kg)灌胃DM大鼠6周,可以减少DM大鼠生精细胞凋亡,增加附睾精子数量,提高精子活动率。  相似文献   

3.
目的探讨人膀胱出口梗阻(BOO)后逼尿肌酶学和形态学的变化及意义。方法采集8例无BOO膀胱肿瘤患者及8例伴BOO良性前列腺增生患者的膀胱逼尿肌肌条,分别对组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、Ca2+Mg2+-ATP酶活性进行测定,并做电镜观察。结果对照组膀胱逼尿肌组织中SOD活性(20.39±2.02)U/mg蛋白、NOS活性(1.81±0.38)U/mg蛋白,Ca2+Mg2+-ATP酶活性(1.47±0.43)μmol P i/mg蛋白,BOO组则分别为(12.77±2.62)U/mg蛋白,(1.36±0.22)U/mg蛋白,(0.97±0.33)μmol P i/mg蛋白(P<0.05),BOO组MDA含量(1.70±0.22)nmol/mg蛋白低于BOO组(2.42±0.69)nmol/mg蛋白(P<0.05)。电镜观察BOO组逼尿肌细胞中粗面内质网明显扩张、脱颗粒,线粒体水肿明显、空泡变性和线粒体内嵴减少、消失,有的细胞内可见大量溶酶体。结论人BOO后缺血再灌注参与人逼尿肌功能失代偿的演化过程,减少自由基产生和避免过度超氧化反应仍是防止或减缓BOO后膀胱逼尿肌发生一系列病理变化的关键。  相似文献   

4.
目的:研究胰高血糖素样肽-1(GLP-1)类似物利拉鲁肽对糖尿病(DM)大鼠阴茎海绵体内皮型一氧化氮合酶(e NOS)表达的影响,探讨利拉鲁肽对DM勃起功能障碍(DED)大鼠勃起功能的作用。方法:取6周龄雄性SD大鼠,分为正常对照组(NC,n=10)与实验组(n=20),实验组构建DM大鼠模型,随机将实验组分为DM组(n=8)与GLP-1组(n=8)。干预12周后,检测各组空腹血糖(FPG)、空腹胰岛素(FINS)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、睾酮、白介素-6,计算胰岛素敏感指标Homa-IR与Homa-β,比较各组大鼠勃起功能;Western印迹检测各组大鼠阴茎海绵体组织Akt/p-Akt、e NOS/p-e NOS的表达。结果:DM组大鼠勃起次数(0.90±1.14)及勃起率(37.5%)较GLP-1组(2.90±1.53,25.0%)、NC组(4.20±1.05,100%)均明显减少(P0.05);GLP-1组大鼠勃起率及勃起次数亦少于NC组大鼠(P0.05)。免疫荧光染色提示e NOS主要表达在海绵体血管、血窦内皮细胞的细胞质中,DM组、GLP-1组e NOS蛋白表达水平显著低于正常对照组(P0.05),且GLP-1组明显高于DM组(P0.05)。DM、GLP-1组大鼠阴茎组织e NOS/p-e NOS表达水平较NC组明显下降(P0.01或0.05)。与DM组相比,GLP-1组大鼠阴茎组织p-e NOS表达水平明显升高(P0.05)。3组大鼠阴茎组织Akt比较无显著差异(P0.05)。DM、GLP-1组大鼠阴茎组织p-Akt表达水平较NC组明显下降(P0.01或0.05)。与DM组相比,GLP-1组大鼠阴茎组织p-e NOS表达水平明显升高(P0.05)。结论:GLP-1可能通过调节Akt/e NOS信号通路,保护阴茎海绵体组织内皮细胞功能,改善DED大鼠的勃起功能,提示GLP-1的使用可能是将来治疗和预防DED的重要方法之一。  相似文献   

5.
目的:一氧化氮(NO)是阴茎勃起的关键因子,其生成主要由一氧化氮合酶(NOS)调节,而磷酸化Erk1/2(P-Erk1/2)和磷酸化Akt1(P-Akt1)均能调节一氧化氮合酶(NOS)的表达及活性从而影响阴茎勃起。本实验研究P-Erk1/2和P-Akt1激酶在老年大鼠阴茎海绵体中的表达,探讨其在老年大鼠勃起功能障碍(ED)发生中的可能作用。方法:A组(2月龄)和B组(18月龄)雄性SD大鼠各10只,测其血清睾酮(T),免疫组化和RT-PCR方法检测大鼠阴茎海绵体中P-Erk1/2和P-Akt1的表达水平。结果:血清T值在B组[(4.73±0.94)nmol/L]较A组[(9.57±1.57)nmol/L]显著下降(P<0.05)。P-Erk1、P-Erk2的mRNA和P-Erk1/2蛋白的相对表达量(积分光密度值IA)在B组(0.95±0.06、0.92±0.05、32.09±8.45)较A组(0.47±0.09、0.61±0.11、7.50±1.81)显著升高(P<0.05);P-Akt1的mRNA和P-Akt1蛋白的相对表达量在B组(0.94±0.05、10.93±3.06)与A组(0.97±0.04、11.67±5.61)无显著差异(P>0.05)。结论:P-Erk1/2的过表达可能是老年性ED发生的机制之一。  相似文献   

6.
目的:研究肺纤维化对大鼠勃起功能的影响及其机制。方法:12周雄性SD大鼠40只,随机分为4组:正常对照4周组(A组)、6周组(B组)和肺纤维化大鼠4周组(C组)、6周组(D组)各10只,分别用生理盐水(A、B组)及博莱霉素(5 mg/kg)气管内注入,饲养4周(A、C组)、6周(B、D组)后,测定大鼠血清睾酮、动脉血气分析、阴茎海绵体内压/平均颈动脉压(ICP/MAP),取阴茎标本测定NOS活性及cGMP含量,实时荧光PCR检测eNOS、iNOS和nNOS的mRNA在阴茎海绵体的表达,W estern印迹检测阴茎海绵体eNOS蛋白的表达。结果:电刺激的3 V,5 V C组ICP/MAP×100(16.37±2.19,27.19±3.18)较A组(30.78±2.66,50.09±6.97)显著降低(P<0.05),D组ICP/MAP×100,3 V,5 V(10.17±1.31,17.40±1.74)较B组(31.45±3.07,51.23±7.23)显著降低(P<0.05),D组ICP/MAP×100值较C组显著降低(P<0.05)。C组PaO2(75.50±13.87)mmHg较A组(103.80±6.88)mmHg显著降低(P<0.05),D组PaO2(83.60±5.50)mmHg较B组(102.70±5.77)mHg显著降低(P<0.05)。C组血清睾酮水平(391.1±264.7)ng/d l较A组(175.9±53.0)ng/d l显著升高(P<0.05),D组血清睾酮水平(745.4±408.8)ng/d l较B组(177.8±52.3)ng/d l显著升高(P<0.05),同时D组血清睾酮水平较C组显著升高(P<0.05)。C组NOS活性及cGMP含量[(1.50±0.14)U/mg prot,(35.69±3.64)pmol/mg]较A组[(2.66±0.39)U/mg prot,(51.10±7.22)pmol/mg]显著降低(P<0.05),D组NOS活性及cGMP含量[(1.40±0.20)U/mg prot,(34.55±4.30 pmol/mg)]较B组[(2.75±0.36)U/mg prot,(52.15±6.86)pmol/mg]显著降低(P<0.05),C组与D组比较NOS活性及cGMP含量无显著性差异(P>0.05)。C组eNOS蛋白表达量(0.79±0.01)较A组(0.87±0.01)显著降低(P<0.01),D组eNOS蛋白表达量(0.71±0.02)较B组(0.88±0.01)显著降低(P<0.05),D组较C组eNOS蛋白表达量显著降低(P<0.05)。C组eNOS mRNA表达量(4.46±0.92)较A组(2.61±0.68)显著升高(P<0.05),D组eNOS mRNA(2.79±0.60)表达量与B组(2.69±0.65)无显著性差异(P>0.05),nNOS及iNOS的mRNA表达量在A、B组与C、D组间均无显著性差异(P>0.05)。结论:肺纤维化可通过抑制阴茎海绵体eNOS蛋白的表达、降低总NOS活性及cGMP含量等机制抑制阴茎勃起功能。  相似文献   

7.
黄芪对大鼠睾丸扭转/复位模型保护作用的研究   总被引:2,自引:2,他引:0  
目的:探讨黄芪注射液对大鼠睾丸扭转/复位模型的保护作用。方法:将30只健康雄性Wistar大鼠分为3组。分别为假手术对照组(A组,n=10);睾丸扭转/复位组(B组,n=10);睾丸扭转/复位+腹腔内注射黄芪注射液组(C组,n=10)。按Turner法建立睾丸扭转模型,喂养至术后7d处死,切取扭转侧睾丸检测凋亡指数(AI)及谷胱甘肽过氧化物酶活力及丙二醛含量。结果:A、B、C三组扭转侧睾丸AI分别为5.82±1.21、36.18±8.40、20.39±3.57,B、C组明显高于对照组(P(0.05),B组明显高于C组(P(0.05)。A、B、C三组扭转侧睾丸谷胱甘肽过氧化物酶活力分别为48.03±2.01、30.93±1.25、38.44±1.06U/mg;丙二醛含量分别为1.43±0.17、3.98±0.36、2.57±0.53nmol/ml,三组之间比较均有显著性差异(P(0.05)。结论:黄芪注射液可明显减少扭转侧睾丸生殖细胞凋亡,保护谷胱甘肽过氧化物酶活力,减轻脂质过氧化程度。  相似文献   

8.
目的探讨雌激素对大鼠门静脉高压性胃病的影响及其机制。方法40只SD大鼠被随机分为4组:P+E组接受门静脉主干结扎+雌激素;P组仅接受门静脉主干结扎;S+E组接受假手术+雌激素;S组仅接受假手术。所有大鼠维持相应处理14 d后处死,处死前1h予2 ml 99%乙醇灌胃。用激光多普勒仪检测胃黏膜血流量,计算胃黏膜损伤程度。用动态一氧化氮(NO)检测仪检测胃黏膜NO含量等。结果P+E组胃黏膜血流量(103±14)U显著高于其他3组(P<0.05)。胃黏膜损伤指数:P+E组(0.28±0.17)、P组(0.21±0.08)和S+E组(0.21±0.12)组均显著高于S组(0.11±0.06),P<0.05。P+E组的NO测量值(153±23)nmol/L显著高于P组(123±14) nmol/L、S+E组(116±18)nmol/L和S组(104±15)nmol/L,P<0.05。结论雌激素加重门静脉高压症大鼠胃黏膜的损伤,可能与其促进胃黏膜内NO的产生有关。  相似文献   

9.
伐地那非治疗老年糖尿病性勃起功能障碍疗效分析   总被引:1,自引:1,他引:0  
目的:观察伐地那非治疗老年糖尿病(DM)性勃起功能障碍(ED)患者的临床疗效和安全性。方法:选择男性科门诊100例老年ED患者,其中DMED40例,非DMED60例。2组均使用伐地那非,首次剂量20mg,以后维持剂量10mg,1次/周,连续8周。采用国际勃起功能问卷勃起功能评分(IIEF-5)和勃起质量量表问卷(EQS)对患者勃起功能状况进行评估。结果:治疗前后,DMED组患者IIEF-5和EQS评分值分别为(8.1±0.5)分,(18.9±0.2)分(P<0.01);(9.1±1.3)分,(25.1±1.4)分(P<0.01);非DMED组患者评分值分别为(10.1±0.3)分,(21.1±0.2)分(P<0.01),(10.1±1.7)分,(34.2±1.2)分(P<0.01),2组间统计学处理差异有显著性(P<0.05)。DMED组显效17例(42.5%),有效9例(22.5%),总有效率65%。非DMED组显效28例(46.7%),有效16例(26.7%),总有效率73.3%。DMED和非DMED2组间统计学处理差异有显著性(P<0.05)。结论:伐地那非治疗能显著改善老年DMED患者的勃起能力,提高生活质量。  相似文献   

10.
He SH  Wei AY  Ye TY  Yang Y  Luo XG  Liu Y  Zhang T 《中华男科学杂志》2011,17(10):913-917
目的:了解降钙素基因相关肽(CGRP)对糖尿病性ED大鼠阴茎海绵体平滑肌细胞表型转化的影响。方法:利用链脲佐菌素建立糖尿病及糖尿病性ED大鼠模型。阴茎海绵体平滑肌细胞原代培养,并进行免疫细胞化学染色鉴定。实验分为2组:正常对照组和糖尿病性ED大鼠组。不同浓度(0、10,60,100 nmol/L)CGRP作用24h后,利用qRT-PCR检测各组细胞碱性调宁蛋白和骨桥蛋白mRNA的表达。结果:各组原代培养阴茎海绵体平滑肌细胞α-肌动蛋白阳性细胞率为(95.94±0.03)%。与正常对照组比较,糖尿病组ED大鼠阴茎海绵体平滑肌细胞碱性调宁蛋白mRNA表达显著减少(4.41±0.29 vs 10.35±0.62,P<0.01),而骨桥蛋白mRNA表达水平显著上调(5.28±0.32 vs 1.32±0.24,P<0.01)。当CGRP作用的终浓度为100 nmol/L时,糖尿病组大鼠阴茎海绵体平滑肌细胞经CGRP作用后,与未经其作用相比,碱性调宁蛋白mRNA表达显著上调(6.90±0.22 vs 4.41±0.29,P<0.01),而骨桥蛋白mRNA表达水平显著减少(3.26±0.31 vs 5.28±0.32,P<0.01)。结论:CGRP可使糖尿病性ED大鼠阴茎海绵体平滑肌细胞表型从合成型向收缩型转化。  相似文献   

11.
Background : We investigated the vasopressor hormone response following mesenteric traction (MT) with hypotension due to prostacyclin (PGI2) release in patients undergoing abdominal surgery with a combined general and epidural anesthesia. Methods : In a prospective, randomized, placebo-controlled study we administered 400 mg ibuprofen (i.v.) in 42 patients scheduled for abdominal surgery. General anesthesia was combined with epidural anesthesia (T4-L1). Before as well as 5, 15, 30, 45, and 90 min after MT we recorded plasma osmolality, hemodynamics and measured 6-keto-PGFlα (stabile metabolite of PGI2), TXB2 (stabile metabolite of thromboxane A2) active renin, and arginine vasopressin (AVP) plasma concentrations by radioimmunoassay. Catecholamine levels were assessed by high-pressure liquid chromatography (HPLC) with electrochemical detection. Results : Following MT, arterial hypotension occurred along with a substantial PGI2 release. This was completely abolished by ibuprofen administration. Although plasma levels of 6-keto-PGF (1133 (708) vs. 60 (3) ng/L, median (median absolute deviation), P=0.0001, placebo vs. ibuprofen) remained significantly elevated, blood pressure was restored within 30 min after MT in the placebo group. At the same point in time plasma concentrations of TXB2 (164 (87) vs. 58 (1) ng/L, P=0.0001), epinephrine (46 (33) vs. 14 (6) ng/L, P=0.001), AVP (41 ± (18) vs. 12 (7) ng/L, P=0.0004), and active renin (27 (12) vs. 12 (4) ng/L, P = 0.001) were significantly higher in placebo-treated patients. Conclusion : Under combined general and epidural anesthesia arterial hypotension following MT due to endogenous PGI2 release is associated with enhanced release of AVP, active renin, epinephrine and thromboxane A2, presumably contributing to hemodynamic stability within 30 min after MT.  相似文献   

12.
Don Dame 《Artificial organs》1996,20(5):613-617
Abstract: Virtually all blood pumps contain some kind of rubbing, sliding, closely moving machinery surfaces that are exposed to the blood being pumped. These valves, internal bearings, magnetic bearing position sensors, and shaft seals cause most of the problems with blood pumps. The original teaspoon pump design prevented the rubbing, sliding machinery surfaces from contacting the blood. However, the hydraulic efficiency was low because the blood was able to "slip around" the rotating impeller so that the blood itself never rotated fast enough to develop adequate pressure. An improved teaspoon blood pump has been designed and tested and has shown acceptable hydraulic performance and low hemolysis potential. The new pump uses a nonrotating "swinging" hose as the pump impeller. The fluid enters the pump through the center of the swinging hose; therefore, there can be no fluid slip between the revolving blood and the revolving impeller. The new pump uses an impeller that is comparable to a flexible garden hose. If the free end of the hose were swung around in a circle like half of a jump rope, the fluid inside the hose would rotate and develop pressure even though the hose impeller itself did not "rotate"; therefore, no rotating shaft seal or internal bearings are required.  相似文献   

13.
Background : Our objective was to determine whether administration of propranolol or verapamil modifies the hemodynamic adaptation to continuous positive-pressure ventilation (CPPV), in particular the regional distribution of cardiac output (CO).
Methods : General hemodynamics and regional blood flows assessed by microsphere technique (15 (μm) were recorded in 16 anesthetized pigs during spontaneous breathing (SB) and CPPV with 8 cm H2O end-expiratory pressure (CPPV8) before and after intravenous administration of propranolol (0.3 mg · kg−1 followed by 0.15 mg · kg−1 · h−1, n=8) or verapamil (0.1 mg · kg−1 followed by 0.3 mg · kg−1 · h−1, n=8).
Results : CPPV8 depressed CO by 25% without shifts in its relative distribution with the exception of a noteworthy increase in adrenal perfusion. Propranolol increased arterial blood pressure, and due to a fall in heart rate, CO dropped by 25%. The kidneys and, to a lesser extent, the splanchic region and central nervous system received increased fractions of the remaining CO at the expense of skeletal muscle flow. Similar patterns were seen during SB and CPPV8 such that the combination of propranolol and CPPV8 depressed CO by 50%. The circulatory effects of verapamil were less evident but myocardial perfusion tended to increase.
Conclusions : The combination of propranolol or verapamil with CPPV does not result in any specific hemodynamic interaction in anesthetized pigs, except that the combined effect of propranolol and CPPV may severely reduce CO.  相似文献   

14.
Background : Inhibitory effects of volatile anaesthetics on platelet aggregation have been demonstrated in several studies. However, the influence of volatile anaesthetics on intracoronary platelet adhesion has not been elucidated so far.
Methods : Isolated hearts of guinea pigs were perfused with buffer in the absence or presence of volatile anaesthetics (0.5 and 1 MAC) at constant coronary flow rates of 5 ml/min for 25 min, then 1 ml/min for 30 min and again 5 ml/min for 10 min. Before, during and after low-flow perfusion, a bolus of human platelets was applied into the coronary system. To simulate thrombogenic conditions, 0.3 U/ml human thrombin was infused during low-flow perfusion and reperfusion. The number of platelets sequestered to the endothelium was calculated from the difference between coronary in- and output of platelets. The myocardial production of lactate and consumption of pyruvate and coronary perfusion pressure were also determined.
Results : At a flow rate of 5 ml/min only about 3% of the applied platelets did not emerge from the coronary system, in any group. In contrast, 13.1±1.2% (mean±SEM) of infused platelets became adherent in low-flow perfusion in the control group without anaesthetic. The adherence was reduced with each 1 MAC isoflurane (to 6.2±1.2%), sevoflurane (to 4.4±0.9%) or halothane (to 3.2±1.5%) (each P <0.05 vs. control). Volatile anaesthetic, 0.5 MAC, did not inhibit platelet adhesion to a statistically significant extent in any case. Perfusion pressure and metabolic parameters were not statistically different between the control and the hearts exposed to anaesthetics.
Conclusion : Volatile anaesthetics in a concentration of 1 MAC can reduce the adhesion of platelets in the coronary system under reduced flow conditions. This action does not arise from vasodilation or inhibition of ischaemic stress.  相似文献   

15.
Abstract: A variety of protein-bound or hydrophobic substances, accumulating as a result of pathologic conditions such as exogenous or endogenous intoxications, are removed poorly by conventional detoxification methods because of low accessibility (hemodialysis), insufficient adsorption capabilities (hemosorption), low efficiency (peritoneal dialysis), or economic limitations (high-volume plasmapheresis). Combining advantages of existing methods with microspheric technology, a module-based system was designed. Major operating parameters of the latter can be modified to allow for adjustment to individual clinical situations. An extracorporeal blood circuit including a plasmafilter is combined with a secondary high-velocity plasma circuit driven by a centrifugal pump. Different microspheric adsorbers can be combined in one circuit or applied in sequence. Thus, a prolonged treatment can be tailored using specially designed selective adsorber materials. Comparing this system with existing methods (high-flux hemodialysis, molecular adsorbent recycling system), results from our in vitro studies and animal experiments demonstrate the superior efficiency of substance removal.  相似文献   

16.
Background: Halothane inhibits in vitro and in vivo activity of cytochrome P-450 (CYP) 2E1. There are several fluorinated volatile anaesthetics besides halothane, and most of them are defluorinated by CYP2E1. It is unclear whether other fluorinated anaesthetics inhibit the in vivo activity of CYP2E1.
Methods: We compared the inhibitory effects of therapeutic concentrations of four inhalational anaesthetics, halothane, enflurane, isoflurane, and sevoflurane, on chlorzoxazone metabolism in rabbits receiving artificial ventilation.
Results: All four inhalational anaesthetics decreased arterial blood pressure and increased plasma chlorzoxazone concentration. However, no significant differences in the plasma chlorzoxazone concentration were found between the four anaesthetics. The estimated chlorzoxazone clearance increased after beginning inhalation with all four agents, but no significant difference in clearance was noted between agents.
Conclusions: At therapeutic concentrations, the in vivo inhibitory effect on chlorzoxazone metabolism was similar for all four inhalational anaesthetics examined, even though their chemical characteristics and extent of hepatic metabolism differ considerably.  相似文献   

17.
Abstract Immunoadsorption (1A) therapy with tryptophan (TR-350) or phenylalanine (PH-350) adsorbents has been used to reduce the concentration of serum antibodies in human lymphocyte antigen (HLA)-immunized patients. Other forms of plasma purification have been reported to reduce the level of fibrinogen, which affects the blood properties. In this study we investigated the effects of IA therapy using both adsorbents on plasma fibrinogen and immunoglobulins G and M in 13 patients (8 patients were treated with TR-350, and 5 patients were treated with PH-350). During each session 1 plasma volume (2.8 ± 0.4 L of plasma) was processed through the immunocolumn and then returned to the patient together with the blood cells. Compared with the pretreatment values, the plasma fibrinogen, IgG, and IgM concentrations were significantly reduced after IA therapy (p < 0.01 for TR-350; p < 0.04 for PH-350). There was a positive correlation between the degree of reduction of plasma proteins and the number of IA treatments given. A nonpara-metric test (Wilcoxon's signed-rank test or the Mann-Whitney test) was used for statistical analysis. We conclude from our study that IA therapy effectively lowers the plasma levels of fibrinogen, IgG, and IgM and thus can be considered a valuable alternative to other blood purification methods.  相似文献   

18.
Background: Obesity is increasing globallly, including in the formerly "Eastern Bloc" countries. Methods: A survey was made of obesity and bariatric surgery. Results: In the 8 East and Central European countries studied, with total population 300 million, roughly 43% of the population was overweight (BMI 25-30), 23% obese (BMI > 30), with about 15 million people morbidly obese (BMI > 40). From 0-10 morbidly obese individuals/100,000/year undergo bariatric surgery. Conclusion: Most countries were found to provide inadequate treatment for obesity.The majority of the morbidly obese are not treated effectively. However, health-care awareness of obesity and bariatric surgeons are slowly increasing.  相似文献   

19.
20.
Abstract: Photopheresis is a technique in which peripheral blood mononuclear cells, in the presence of a photoacti-vatable compound, are exposed extracorporeally to ultraviolet A light and reinfused, inducing a host autoregula-tory immune response. Experimental work and ongoing clinical studies are helping to define the role of this novel, safe, and non-toxic immunomodulating technology in the field of transplantation.  相似文献   

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