共查询到20条相似文献,搜索用时 62 毫秒
1.
补肾行气活血法在骨髓基质干细胞诱导软骨细胞修复兔关节软骨缺损中的作用 总被引:1,自引:0,他引:1
[目的]探讨补肾行气活血法在骨髓基质于细胞诱导软骨细胞修复兔关节软骨缺损中的作用. [方法]将胶原凝胶包埋的骨炎定含药血清培养的骨髓基质干细胞诱导的软骨细胞和异体软骨细胞接种CPPf/PLLA支架构建的复合物体外培养3周,行倒置显微镜和扫描电镜观察,并将复合物异体移植入兔关节软骨缺损,术后4、8、12周取材,从大体、组织学和Ⅱ型胶原免疫组织化学分别对再生软骨组织进行评价. [结果]诱导软骨细胞组在支架内分布均匀、透明软骨样组织的形成、表面光滑度、与周围组织整合程度及基质内有Ⅱ型胶原分布等方面明显优于软骨细胞组. [结论]补肾行气活血法在关节软骨缺损修复的作用中较传统的方法有其优越性. 相似文献
2.
胶原凝胶包埋软骨细胞接种CPPf/PLLA支架体外构建组织工程软骨 总被引:3,自引:0,他引:3
目的:探讨软骨细胞均匀、高效种植于三维支架的细胞接种方法。方法:将胶原凝胶包埋的软骨细胞整合入CPPf/PLLA三维支架并进行体外培养,细胞计数检测细胞粘附情况,倒置显微镜观察细胞在支架内分布的均一性,组织形态学检测细胞-胶原凝胶-支架复合物形成软骨组织的情况。结果:超过90%的种植细胞能有效、均匀种植于CPPf/PLLA支架,体外培养3周的复合物能形成较成熟的工程化软骨组织。结论:胶原凝胶包埋软骨细胞三维接种能有效提高组织工程软骨的体外构建质量,同时结合了两种材料的优势。 相似文献
3.
目的:探讨软骨细胞均匀、高效种植于三维支架的细胞接种方法.方法:将胶原凝胶包埋的软骨细胞整合入CPPf/PLLA三维支架并进行体外培养,细胞计数检测细胞粘附情况,倒置显微镜观察细胞在支架内分布的均一性,组织形态学检测细胞-胶原凝胶-支架复合物形成软骨组织的情况.结果:超过90%的种植细胞能有效、均匀种植于CPPf/PLLA支架,体外培养3周的复合物能形成较成熟的工程化软骨组织.结论:胶原凝胶包埋软骨细胞三维接种能有效提高组织工程软骨的体外构建质量,同时结合了两种材料的优势. 相似文献
4.
软骨细胞-胶原海绵复合移植修复软骨缺损的形态学研究 总被引:4,自引:1,他引:3
目的:研究软骨细胞-胶原海绵复合移植修复软骨缺损的疗效。方法:软骨细胞取自3日龄异体幼兔,体外培养后接种于胶原海绵支架上,再移植于兔膝关节全层软骨缺损处。在同一大白兔双膝关节内外髁分3组作自身对照研究,股骨内髁作软骨细胞-胶原海绵复合移植组,右膝股骨外髁作单纯胶原海绵移植组,左膝股骨外髁作空白对照组。移植术后第4、8、12、16、20周分批处死,进行大体、组织学和超微结构观察。结果:软骨细胞-胶原海绵复合移植组缺损处为软骨性修复,而单纯胶原海绵移植组和空白对照组为纤维性修复。结论:运用软骨组织工程的原理,以胶原海绵作为支架材料的异体软骨细胞移植可修复兔关节软骨缺损,为治疗关节软骨缺损提供了一种有效的方法。 相似文献
5.
目的:应用组织工程学技术,体外初步构建组织工程化人工关节软骨。方法:制备三维多孔软骨支架材料CPP/PLLA,体外诱导兔MSCs向软骨细胞表型分化,免疫组织化学染色检测软骨特异性Ⅱ型胶原表达,将诱导细胞与软骨支架材料CPP/PLLA复合,体外培养构建人工关节软骨,1周后终止培养,扫描电镜观察组织工程化人工软骨的微观结构;同时将构建人工软骨移植于兔大腿皮下,3周后处死动物,甲苯胺蓝染色观察。结果:扫描电镜观察可见该复合材料CPP/PLLA为高孔隙率的网状、连通、微孔结构,微孔分布均匀,孔径大小为300~400μm之间;兔MSCs经体外软骨表型定向诱导后,Ⅱ型胶原免疫组化染色阳性。诱导后的MSCs可在支架材料内良好贴附生长,细胞被分泌的胶原基质包裹;从体内获取的培养物组织切片观察可见大量的软骨细胞生成,甲苯胺蓝染色阳性。结论:经软骨起源诱导后的MSCs与CPP/PLLA复合培养可以构建自体软骨移植的替代物,为应用软骨组织工程方法修复关节软骨缺损和功能重建提供一种新材料,具有较大的潜在应用价值。 相似文献
6.
聚磷酸钙纤维和左旋聚乳酸软骨支架复合自体骨髓间充质干细胞构建组织工程化人工关节软骨 总被引:1,自引:0,他引:1
目的应用组织工程学技术,体外初步构建组织工程化人工关节软骨。方法制备三维多孔软骨支架材料CPP/PLLA,体外诱导兔MSCs向软骨细胞表型分化,免疫组织化学染色检测软骨特异性Ⅱ型胶原表达,将诱导细胞与软骨支架材料CPP/PLLA复合,体外培养构建人工关节软骨,1周后终止培养,扫描电镜观察组织工程化人工软骨的微观结构;同时将构建人工软骨移植于兔大腿皮下,3周后处死动物,甲苯胺蓝染色观察。结果扫描电镜观察可见该复合材料CPP/PLLA为高孔隙率的网状、连通、微孔结构,微孔分布均匀,孔径大小为300~400Ⅳn之间;兔MSCs经体外软骨表型定向诱导后,Ⅱ型胶原免疫组化染色阳性。诱导后的MSCs可在支架材料内良好贴附生长,细胞被分泌的胶原基质包裹;从体内获取的培养物组织切片观察可见大量的软骨细胞生成,甲苯胺蓝染色阳性。结论经软骨起源诱导后的MSCs与CPP/PLLA复合培养可以构建自体软骨移植的替代物,为应用软骨组织工程方法修复关节软骨缺损和功能重建提供一种新材料,具有较大的潜在应用价值。 相似文献
7.
纤维蛋白凝胶和脱钙骨基质支架材料复合软骨细胞修复兔膝关节软骨缺损的实验研究 总被引:3,自引:2,他引:1
目的:探讨纤维蛋白凝胶和脱钙骨基质支架材料复合软骨细胞作为软骨组织工程支架的可行性及有效性,并为后续研究可注射性材料做基础。方法:体外分离培养软骨细胞后接种到纤维蛋白凝胶和脱钙骨基质支架材料体外培养4周,然后植入兔膝关节软骨缺损区继续培养4、8、12周后取材,分别行大体、组织学、Ⅱ型胶原免疫组织化学观察。并进行Wakitani评分,观察其体内修复关节缺损效果。结果:大体观察4周后,实验组软骨缺损区可有乳白色组织修复,12周可修复完全,并无明显凹凸感。光镜下8周可见大量软骨细胞修复,并在TB染色下见Ⅱ型胶原比4周时明显增多。12周时软骨陷窝结构形成,细胞形态排列及Ⅱ型胶原与正常软骨组织相近。结论:纤维蛋白凝胶和脱钙骨基质支架材料复合软骨细胞可以作为软骨组织工程支架材料,能够用于再生修复软骨的缺损。并为构建可注射性修复材料提供途径。 相似文献
8.
目的 评估同种异体组织工程软骨修复兔膝关节全层软骨缺损的有效性。方法分离收集成年新西兰大白兔软骨细胞进行体外培养。建立双侧兔膝关节软骨缺损模型,用去端肽胶原(atelocollagen)凝胶与所培养的异体兔关节软骨细胞共同植入兔膝关节软骨缺损处,并设对照组。分别于手术后4周、8周观察大体标本以及组织学修复结果,并进行Wakitan的评分,评估此方法的有效性。结果大体观察结果表明,与对照组相比,实验组缺损处由软骨组织修复而对照组缺损处由纤维样组织填充。组织学观察可以见到实验组关节软骨缺损处有密集的软骨细胞而对照组关节缺损处只有纤维细胞无软骨细胞。结论通过短期观察表明以同种异体软骨细胞-去端肽胶原复合物修复全层软骨缺损的方法是有效可行的,为其进一步临床应用提供了参考。 相似文献
9.
Ⅰ型胶原负载骨髓基质细胞修复兔膝关节软骨缺损 总被引:10,自引:3,他引:7
目的探讨同种异体骨髓基质细胞(MSCs)-Ⅰ型胶原复合移植修复关节软骨缺损的疗效。方法构建Ⅰ型胶原支架,将体外培养的兔MSCs吸附于该支架上培养,检测支架对MSCs的吸附及增殖的影响,再移植于兔膝关节全层软骨缺损处,分批取材,进行大体、组织学观察。结果MSCs在Ⅰ型胶原支架内贴附良好,分泌胞外基质,MSCs-胶原复合移植组软骨缺损处在术后12周为透明软骨样修复,空白对照组为纤维瘢痕样修复。结论Ⅰ型胶原支架与MSCs相容性良好,MSCs-胶原复合移植可达到透明软骨样修复软骨缺损。 相似文献
10.
关节软骨细胞三维支架双相接种体外生成软骨组织的观察 总被引:17,自引:0,他引:17
目的联合应用自制生物凝胶和三维支架材料,建立组织工程种子细胞的三维支架双相接种技术,并对其构建生成组织工程关节软骨的效果进行初步观察。方法酶消化法分离幼兔关节软骨细胞,于培养瓶中接种、培养,收集第1代软骨细胞,以2.5×107/ml加入液态的自制生物凝胶液中充分混均,制成细胞-凝胶混悬液,接种于CPPf/PLLA(caliumpolyphosphatefiber/poly-L-lacticacid)三维支架材料中,经固化后形成工程化组织块,体外连续培养4周。行大体、倒置显微镜以及组织学、Ⅰ型和Ⅱ型胶原免疫组化光镜观察,二甲基亚甲蓝显色法定量检测硫酸糖胺多糖含量。结果(1)细胞-凝胶混悬液接种后完全充满CPPf/PLLA支架的孔隙,固化后三者很好地形成一个工程化组织整体。构建的工程化组织块在培养过程中不仅能够保持其初始外形,而且能保持种子细胞稳定的三维均相分布,无细胞脱落现象。同时,硬度亦不断增加,有弹性,表面湿润、光滑。(2)随着培养时间的延长,软骨组织的形成是由外周向中心进行。培养2周后,逐渐形成富含Ⅱ型胶原和蛋白聚糖、具有典型软骨组织结构的成熟工程化软骨。同时,Ⅰ型胶原逐渐转为阴性,支架材料亦不断降解。4周时硫酸糖胺多糖含量平均为(15.70±2.00)mg/g(湿重),为天然关节软骨的30%以上。结论种子细胞的三维支架双相 相似文献
11.
【摘要】〓乳腺癌是危害我国女性健康的头号杀手,尽管近年来辅助化疗的研究进展突飞猛进,但临床中仍有不少问题未能明确,如辅助化疗的合适人群、化疗的开始时间、蒽环及紫杉类的地位和用法、强化维持治疗的作用、疗效及预后的生物标志物等。本文结合乳腺癌辅助化疗在临床上的常见问题和2015年各大乳腺癌会议阐述乳腺癌辅助化疗的最新进展。 相似文献
12.
13.
O'Rourke RW Kay T Scholz MH Diggs B Jobe BA Lewinsohn DM Bakke AC 《Obesity surgery》2005,15(10):1463-1468
Background: Obesity affects the regulation of immune and inflammatory responses. This study characterizes differences in peripheral
blood lymphocyte phenotype in obese humans. Methods: Frequencies of lymphocyte subsets among peripheral blood mononuclear
cells were compared between 10 obese (BMI ≥35) and 10 lean subjects, as determined by antibodies directed against cluster
differentiation (CD) markers. Results: Obese patients demonstrated an increased frequency of CD3+CD4+ T-cells (mean difference
12%, P=0.004), a decreased frequency of CD3+CD8+ T-cells (mean difference 9.4%, P=0.016) and an increased frequency of CD3+CD8+CD95+ T-cells (mean difference 13.3%, P=0.032). No other differences among T-cell or monocyte subsets were noted. Conclusions: Obesity is associated with alterations
in frequencies of peripheral CD4+ and CD8+ T-cells and aberrations in the expression of CD95 among CD8+ T-cells. These data
suggest both CD4+ and CD8+ T-cell compartments, as well as the regulation of CD95 expression on CD8+ T-cells, as targets for
further study into obesity's effects on the immune system. 相似文献
14.
15.
目的 研究β—半乳糖苷酶(β—gal)在成骨细胞中的表达状况,为阐明MorquioB综合征的发病机制提供依据。方法 裸鼠各器官和骨组织标本行X-gal染色检测。抽取羊和人骨髓行骨髓基质细胞(BMSCs)培养,分为4组:I:Adv-hBMP-2转染组;Ⅱ:Adv—β—gal转染组;Ⅲ:未转染组;Ⅳ:地塞米松诱导组。分别行X-gal染色和RT-PCR检测β—gal的表达。结果 裸鼠骺板两侧、骨膜内面及松质骨的成骨细胞和破骨细胞可见多量β—gal的表达。未转染BMSCs组有少量β—gal的表达,其他3组细胞的β—gal表达增高。结论成骨细胞和破骨细胞可表达多量β—gal,该两种细胞的β—gal缺乏可能是MorquioB综合征骨骼异常的直接原因。 相似文献
16.
Parvez Sheikh 《The Indian journal of surgery》2012,74(3):217-220
Managing a complex fistula in ano can be a daunting task for most surgeons; largely due to the two major dreaded complications—recurrence & fecal incontinence. It is important to understand the anatomy of the anal sphincters & the aetiopathological process of the disease to provide better patient care. There are quite a few controversies associated with fistula in ano & its management, which compound the difficulty in treating fistula in ano. This article attempts to clear some of those major controversies. 相似文献
17.
18.
C. H. YEUNG T. G. COOPER G. F. WEINBAUER M. BERGMANN G. KLEINHANS† H. SCHULZE‡ E. NIESCHLAG 《International journal of andrology》1989,12(5):384-394
Ligated tubules from the corpus epididymidis of men and monkeys were incubated in medium containing horseradish peroxidase (HRP) as a marker for fluid-phase endocytosis. HRP was localized by light and electron microscopy after 0, 15, 30 and 60 min of incubation. Movement between the cells was prevented by tight junctions, but bypass of this barrier was apparently achieved by an intracellular vesicular mechanism leading to a time-dependent appearance of HRP in the lumen. Uptake of HRP into basal cells and capture by the lysosomal apparatus of principal cells were also observed. HRP-filled vesicles also appeared in the basal, mid and apical cytoplasm of epithelial cells in the caput 1 h after injection of the tracer into the epididymal circulation of the monkey, suggesting that this pathway also operates in vivo. 相似文献
19.
Purpose
Minimally invasive surgery has evolved into single-incision laparoscopic surgery (SILS) in the recent years. Few reports have addressed the practicality of SILS in children. Our current experience with regard to feasibility and effectiveness of SILS in children is presented.Methods
A retrospective review of the operative database for patients operated on using SILS in our department from March 2009 to July 2010 was performed. Data regarding the type of the procedure, age, sex, operative performance, hospital stay, and complications were collected.Main Results
Among 43 patients, cholecystectomy was performed in 11; appendectomy, in 10; unroofing for ovarian cysts, in 5; unroofing for splenic cysts, in 4; oophorectomy, in 6 (ovarian torsion, 2; teratoma, 4); ovary-preserving teratoma excision, in 1; splenectomy, in 1; gonadectomy, in 3; and varicocelectomy, in 2. There were no conversions to standard laparoscopic or open techniques. The only postoperative complication was a wound infection that occurred after an appendectomy.Conclusion
Although currently more expensive, SILS can be performed in children in almost every pediatric surgical procedure that can be accomplished with conventional laparoscopic techniques. The most significant contribution of SILS procedure is cosmesis. Postoperative pain and length of hospital stay were not improved. 相似文献20.
《Archivos de bronconeumologia》2020,56(9):559-563
IntroductionSmoking-attributable mortality (SAM) is a valuable indicator that can be used to characterize the course and health burden of the smoking epidemic. The aim of this paper was to estimate SAM in Spain in 2016 in the population aged 35 and over, using the best available evidence.MethodsA smoking prevalence-dependent analysis based on the estimation of population-attributable fractions was performed. Smoking prevalence (never, former, and current smokers) was calculated from a combination of the Spanish Health Survey (2016) and the European Health Survey (2014); the relative risk of death among current and former smokers was taken from the follow-up of various cohorts; and mortality rates were obtained from National Center for Statistics data. SAM estimates are presented globally, and by sex, age groups, and major disease categories: cancer, cardiometabolic diseases and respiratory diseases.ResultsIn 2016, 56,124 deaths were attributed to tobacco consumption, 84% in men (47,000), and 50% in the population aged over 74 (27,795). Overall, 50% of SAM was due to cancer (28,281), 65% of which was lung cancer. One in 4 attributable deaths (13,849) occurred before the age of 65.ConclusionsOne in 7 deaths in Spain in 2016 were attributable to smoking. This estimation of SAM clearly highlights the great impact of smoking on mortality in Spain, mainly due to lung cancer and chronic obstructive pulmonary disease. 相似文献