首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
目的 观察250ml/m^3一氧化碳(CO)吸入和腹腔给予对脂多糖(LPS)诱导大鼠肠道损伤的影响,以及影响过程中丝裂原活化蛋白激酶p38(p38 MAPK)表达的变化及意义。方法 108只SD大鼠按随机数字表法均分为对照、CO吸入、CO腹腔、LPS注入、LPS+CO吸入及LPS+CO腹腔6组,后3组静脉注入5mg/kg体质量LPS,前3组静脉注入等量生理盐水;1h后,对照及LPS注入组吸入室内空气,CO吸入及LPS+CO吸入组吸入250ml/m^3 CO,CO腹腔及LPS+CO腹腔组以2L/min腹腔通入250ml/m^3CO.观察1、3、6h后,批次(每批6只)放血处死大鼠,取回盲部小肠。酶联免疫吸附法测定细胞间黏附分子-1(ICAM-1)、血小板活化因子(PAF)及白细胞介素-10(IL-10)水平;硫代巴比妥酸法测定丙二醛(MDA)含量;化学比色法测定髓过氧化物酶(MPO)活性;羟胺法测定超氧化物歧化酶(SOD)活性;流式细胞仪检测细胞凋亡率;蛋白印迹法测定磷酸化p38 MAPK表达;光镜观察组织形态学变化。结果 LPS组ICAM-1、PAF、MDA、MPO、细胞凋亡率及磷酸化p38 MAPK表达均显著高于对照、CO吸入及CO腹腔组(P〈0.05或0.01),而IL-10和SOD明显低于该3组(均P〈0.05),肠损伤严重;组内各时间点比较,差异无统计学意义。与相应时间点的LPS组比较,LPS+CO吸入组及LPS+CO腹腔组ICAM-1、PAF、MDA、MPO及细胞凋亡率明显降低(均P〈0.05),而IL-10和SOD显著升高(均P〈0.05),肠损伤减轻,但磷酸化p38MAPK表达进一步增强(P〈0.05);LPS+CO吸入组及LPS+CO腹腔组间及2组内各时间点比较,差异无统计学意义。结论 250ml/m^3 CO吸入和腹腔给予以非时间依赖方式抑制LPS诱导的大鼠肠道过氧化物和促炎细胞因子产生、减少细胞凋亡,增强抗氧化酶、抗炎细胞因子表达而起相似的保护作用;p38MAPK信号转导通路可能参与了这一过程。  相似文献   

2.
七氟醚对内毒素诱导大鼠肺组织氧化应激反应的影响   总被引:11,自引:1,他引:10  
目的 探讨七氟醚对内毒素(LPS)诱导大鼠肺组织氧化应激反应的影响。方法Wistar大鼠48只,体重200-290g,随机分为4组(n=12):对照组(C组)、LPS组(L组)、1.0MAC七氟醚组(S1L组)和1.5MAC七氟醚组(S2L组)。各组大鼠腹腔注射异戊巴比妥钠100mg/kg,麻醉后机械通气。维持呼气末二氧化碳分压在35~45mmHg。L组股静脉注射LPS5mg/kg,C组给予生理盐水1.2ml,S1L组、S2L组注射LPS后分别吸入1.0MAC、1.5MAC七氟醚4h。吸入七氟醚后4h处死大鼠,测定肺组织超氧化物超歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、总一氧化氮合酶(tNOS)水平、诱导型一氧化氮合酶(iNOS)活性及iNOS mRNA、蛋白表达。结果 与C组比较,L组肺组织SOD活性下降,MDA、NO、tNOS水平、iNOS活性及iNOS mRNA及蛋白表达均升高(P〈0.01);与L组比较,S1L组、S2L组肺组织SOD活性升高,MDA、NO、tNOS水平及iNOS mRNA及蛋白表达降低(P〈0.05);S1L组与S2L组上述各项指标比较差异无统计学意义(P〉0.05)。结论 吸入1.0MAC和1.5MAC七氟醚4h可减轻LPS诱导肺组织氧化应激反应。  相似文献   

3.
目的 研究姜黄素预先给药对内毒素诱导大鼠急性肺损伤的作用及其可能机制。方法 48只雄性Wistar大鼠,随机分为4组(n=12),对照组(S组)、姜黄素组(C组)分别静脉注射10%二甲基亚砜(DMSO)2 ml/kg、姜黄素40 mg/kg(溶于DMSO),30 min后静脉注射生理盐水2 ml/kg;内毒素组(L组)、姜黄素预先给药组(C-L组)分别静脉注射10%DMSO 2 ml/kg、姜黄素40 mg/kg,30 min后静脉注射脂多糖(LPS)6 mg/kg。注射LPS后4 h处死动物。每组中6只大鼠在处死前15 min静脉注射伊文思蓝(EB)20mg/kg,用于测定肺组织EB含量,每组的其余大鼠用于测定支气管肺泡灌洗液(BALF)中髓过氧化物酶(MPO)活性和中性粒细胞(PMN)计数及肺组织湿干重比(W/D)、MPO活性、PMN趋化因子-1(CINC-1)mRNA、CINC蛋白表达,并在光镜下观察肺组织病理学改变。结果 与S组比较,L组BALF中MPO、PMN计数和肺组织W/D、EB及MPO、CINC-1 mRNA、CINC蛋白水平升高(P〈0.05或0.01),C组上述指标差异均无统计学意义(P〉0.05);与L组比较,C-L组上述指标均降低(P〈0.05或0.01)。C-L组肺组织病理学损伤较L组减轻。结论 姜黄素40mg/kg预先给药对内毒素诱导急性肺损伤大鼠肺产生一定的保护作用,与下调肺组织CINC-1的表达进而抑制PMN在肺组织的聚集、激活有关。  相似文献   

4.
核因子κB抑制剂对缺血再灌注皮瓣TNF-α和ICAM-1表达的影响   总被引:2,自引:0,他引:2  
目的观察缺血再灌注(I/R)期间,皮瓣肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(1CAM-1)表达及核因子κB(NF—κB)活性抑制剂的影响。方法雄性Wistar大鼠27只,随机分为对照组(A组)、I/R组(B组)及吡咯烷二硫氨基甲酸酯(PDTC)处理组(C组)。制备右下腹岛状皮瓣I/R模型。C组于再灌注前及早期,各静脉注射PDTC 300mg/kg。逆转录-聚合酶链式反应法检测皮瓣再灌注2、6h TNFα、ICAM-1 mRNA表达。测定再灌注12h皮瓣髓过氧化物酶活性(MPO)并行组织学观察。结果B组再灌注2、6h TNF—α、ICAM-1表达较A组明显增加(P〈0.01)。C组再灌注2、6hTNF—α、ICAM-1表达明显低于B组(P〈0.01,P〈0.05);再灌注12h,组织MPO活性及中性粒细胞浸润、水肿情况明显减轻。结论再灌注早期炎症介质表达增加在皮瓣I/R损伤中起重要作用。NF—κB抑制剂可下调TNF—α和ICAM-1转录表达,明显减轻皮瓣I/R损伤。  相似文献   

5.
目的通过建立在体大鼠肺缺血-再灌注(I-R)损伤模型,观察不同时段吸入20ppm一氧化氮(NO)对大鼠肺I-R损伤的作用。方法40只SD大鼠随机分为五组,I组为假手术组,即左侧开胸,游离肺门,但不夹闭肺门;Ⅱ组为I-R组,夹闭左肺门60min后松开,再灌注3h;Ⅲ组为NO预处理组,即在夹闭前,先吸入20ppmNO30min;Ⅳ组为再灌注即刻NO吸入组,即再灌注即刻吸入20ppmNO30min;V组为再灌注30minNO吸入组,即再灌注30min后吸入20ppmNO30min。检测肺组织白细胞介素-10(IL-10)、IL-1β、肿瘤坏死因子α(TNF-α)以及细胞间黏附分子-1(ICAM-1)表达,丙二醛(MDA)和髓过氧化物酶(MPO)含量和肺组织湿干重比。结果吸入NO可在一定程度上抑制肺I-R后IL-1β和TNF-α的生成,降低ICAM-1的上调,减少MDA和MPO生成,同时抑制IL-10生成。其中Ⅳ组MDA、MPO与Ⅲ、Ⅴ组相比增高,但差异无统计学意义。结论不同时段NO吸入对大鼠肺I-R损伤保护作用不同,与再灌注即刻吸入NO相比,NO预处理以及再灌注30min后NO吸入组的保护作用更加完善,为较佳NO吸入时段。  相似文献   

6.
目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)激动剂15-脱氧前列腺素J2(15d—PGJ2)在大鼠肝脏缺血-再灌注损伤中的保护作用及机理。方法建立70%的大鼠肝脏缺血-再灌注损伤模型,40只SD大鼠随机均分为4组:假手术组、缺血-再灌注损伤组(缺血-再灌注组)、15d-PGJ2预处理组(15d-PGJ2组)及15d-PGJ2+GW9662预处理组(15d—PCJ2+GW9662组)。再灌注后,取静脉血检测肝血清酶(ALT、AST)水平,取肝脏组织检测髓过氧化物酶(MPO)活性、NF—κB活性、TNF—α含量和ICAM-1表达。结果与假手术组相比,其余3组血清ALT和AST水平、肝脏组织MPO和NF—JeB活性、TNF—α含量和ICAM-1表达均增加(P〈0.05)。与缺血-再灌注组相比,15d—PGJ2组血清ALT和AST水平、肝脏组织MPO和NF-κB活性、TNF-α含量和ICAM-1表达均明显降低(P〈0.05);而15d-PGJ2+GW9662组与缺血-再灌注组相比有差异但无统计学意义(P〉0.05)。与15d—PGJ2组相比,15小PGJ2+GW9662组血清ALT和AST水平、肝脏组织MPO和NF—κB活性、TNF—α含量和ICAM-1表达明显增加(P〈0.05)。结论PPARγ激动剂15d—PGJ2对肝脏缺血-再灌注损伤有保护作用,其机理可能是通过PPARγ途径抑制NF—κB活性,减少TNF—α和ICAM-1炎症介质的释放实现的。  相似文献   

7.
目的评价N-脱硫酸肝素(NNH)对大鼠移植肺缺血再灌注损伤的影响。方法纯种雄性sD大鼠20只,体重280~350g,随机分为2组(n=10),对照组(C组)再灌注即刻静脉注射生理盐水1.2ml/kg;NNH组再灌注即刻静脉注射NNH12mg/kg。在呼吸机支持和显微镜辅助下,利用非内皮化袖套式方法,建立大鼠左肺移植模型。再灌注2h后取肺静脉血进行血气分析,取移植肺组织,计算肺湿,干重比(W/D),测定髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)、细胞间黏附分子-1(ICAM-1)mRNA的水平,光镜下观察肺组织的病理学变化。结果与C组比较,NNH组肺静脉血氧分压升高,肺W/D、TNF-α、IL-8和ICAM-1 mRNA水平降低(P〈0.05或0.01),肺静脉血二氧分碳分压和MPO活性差异无统计学意义(P〉0.05)。光镜下NNH组肺组织中性粒细胞浸润和水肿程度均比C组轻。结论静脉注射NNH12mg/kg可减轻大鼠移植肺缺血再灌注损伤,与降低肺组织炎性反应有关。  相似文献   

8.
目的探讨交感神经递质去甲肾上腺素对体外培养大鼠kupffer细胞TNF-α、IL-1β炎症相关细胞因子表达的影响。方法分离大鼠kupffer细胞体外分三组培养,经体外培养48h后,给予外源性内毒素(LPS10μg/m1)刺激,同时给予不同浓度的去甲。肾上腺素(0.1μmol/L~10μmol/L)分别作用12h,运用定量逆转录多聚酶链反应检测各处理组TNF-α和IL-1β mRNA的表达,ELISA检测细胞培养上清TNF-α IL-1β蛋白的表达。结果同时给予LPS(10μg/ml)刺激和去甲肾上腺素(1μmol/L及10μmol/L)作用后,kupffer细胞培养上清TNF-α和IL-1β蛋白较LPS组显著增高(P〈0.05);TNF-α mRNA表达分别较LPS组增加50.9%(P〈0.05)和59.1%(P〈0.05);IL-1β mRNA较LPS组表达增加53.7%(P〈0.05)和57.8%(P〈0.05)。结论应激浓度的去甲肾上腺素能增加内毒素诱导大鼠kupffer细胞TNF-α、IL-1β表达,具有促炎效应。  相似文献   

9.
目的:动态观察内毒素耐受大鼠在内毒素血症时肠道炎症反应和免疫功能的变化,探讨内毒素耐受对内毒素血症大鼠肠道的保护作用及其机制。方法:选择健康成年雄性SD大鼠70只,随机分成3组:正常对照组(10只)、内毒素(LPS)组(30只)和内毒素耐受组(30只)。内毒素耐受组大鼠于实验第1日经腹腔注射LPS 0.25 mg/kg,24h后经腹腔注射LPS 0.5 mg/kg,LPS组在上述时间均给予等量生理盐水;实验第5日上述两组大鼠均腹腔注射大剂量LPS 10 mg/kg,于注射大剂量LPS前(0h)和注射后2、4、6、12h取大鼠肠组织制备肠黏膜上清液,用双抗体夹心法测定肠黏膜组织中分泌型免疫球蛋白A(sIgA)的含量,用免疫组化技术检测肠组织细胞间黏附分子-1(ICAM-1)的表达,并与正常对照组进行比较;光学显微镜下观察3组大鼠肠组织病理学变化,并进行病理学评分。结果:内毒素耐受组在给予小剂量LPS后没有出现体重下降。内毒素耐受组肠黏膜中slgA的含量较正常对照组明显升高,6 h最为明显(P〈0.05),LPS组sIgA含量2 h明显升高(P〈0.05),从6 h开始下降(P〈0.05)。内毒素耐受组给予大剂量LPS后肠黏膜ICAM-1的表达没有呈上升趋势,各时间点ICAM-1的表达与正常对照组比较差异有显著性,以6h ICAM-1的表达最低,而LPS组肠黏膜ICAM-1的表达随时间的延长逐渐升高,明显高于内毒素耐受组。肠组织病理学观察可见:LPS组肠黏膜水肿,绒毛脱落,并伴有大量炎性细胞浸润;内毒素耐受组上述表现较LPS组减轻。两组肠黏膜炎症损伤病理学评分[LPS组(3.89±0.45)分;内毒素耐受组(2.65±0.36)分]差异有显著性(P〈0.01)。结论:内毒素耐受可减轻内毒素血症导致的肠道黏膜屏障的损害,升高sIgA水平,减少ICAM-1表达,从而起到肠保护作用。  相似文献   

10.
目的探讨通过低氧诱导因子-1α(hypoxiainduciblefactor-1α,HIF—1α)抑制剂YC-1预先抑制该基因表达对肠缺血,再灌注(ischemia/reperfusion,I/R)致急性肺损伤的影响。方法6周~8周龄健康雄性C57BL/6小鼠36只,采用随机数字表法随机分为3组(每组12只):假手术组(S组)、I/R组和I/R±YC—I预处理组(YC—I组)。YC-1组于术前10min腹腔注入YC-1(1mg/kg),采用夹闭C57BL/6小鼠肠系膜前动脉45min后再灌注6h的方法造成肠YR损伤模型,取小鼠肺标本称重后计算肺湿干重比,苏木素-伊红(hematoxylin-eosin,HE)染色后观察肺组织病理学改变,分光光度法测定髓过氧化物酶(myeloperoxidase,MPO)活性、酶联免疫吸附测定法检测肺组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素(interleukin,IL)-1β表达,反转录-PCR法检测HIF-1α、Toll样受体4(toll—likereceptor4,TLR4)mRNA的表达。结果与I/R组比较,预先抑制HIFqct表达使肺实质水肿及中性粒细胞浸润聚集减少,肺组织病理学损伤减轻,肺湿干重比显著降低(RnOI),MPO活性下调[(1.88±0.82)u/g],TNF-α[(187±20)ng/L)]、IL一1β[(536±54)ng/L)]、HIF-1α、TLR4mRNA的表达水平下降(P〈0.05)。结论YC-1预处理可使肺组织TLR4mRNA表达下调,抑制肠I/R肺组织中促炎细胞因子的释放,明显减轻小鼠肠I/R后急性肺损伤。  相似文献   

11.
Background : We investigated the vasopressor hormone response following mesenteric traction (MT) with hypotension due to prostacyclin (PGI2) release in patients undergoing abdominal surgery with a combined general and epidural anesthesia. Methods : In a prospective, randomized, placebo-controlled study we administered 400 mg ibuprofen (i.v.) in 42 patients scheduled for abdominal surgery. General anesthesia was combined with epidural anesthesia (T4-L1). Before as well as 5, 15, 30, 45, and 90 min after MT we recorded plasma osmolality, hemodynamics and measured 6-keto-PGFlα (stabile metabolite of PGI2), TXB2 (stabile metabolite of thromboxane A2) active renin, and arginine vasopressin (AVP) plasma concentrations by radioimmunoassay. Catecholamine levels were assessed by high-pressure liquid chromatography (HPLC) with electrochemical detection. Results : Following MT, arterial hypotension occurred along with a substantial PGI2 release. This was completely abolished by ibuprofen administration. Although plasma levels of 6-keto-PGF (1133 (708) vs. 60 (3) ng/L, median (median absolute deviation), P=0.0001, placebo vs. ibuprofen) remained significantly elevated, blood pressure was restored within 30 min after MT in the placebo group. At the same point in time plasma concentrations of TXB2 (164 (87) vs. 58 (1) ng/L, P=0.0001), epinephrine (46 (33) vs. 14 (6) ng/L, P=0.001), AVP (41 ± (18) vs. 12 (7) ng/L, P=0.0004), and active renin (27 (12) vs. 12 (4) ng/L, P = 0.001) were significantly higher in placebo-treated patients. Conclusion : Under combined general and epidural anesthesia arterial hypotension following MT due to endogenous PGI2 release is associated with enhanced release of AVP, active renin, epinephrine and thromboxane A2, presumably contributing to hemodynamic stability within 30 min after MT.  相似文献   

12.
Don Dame 《Artificial organs》1996,20(5):613-617
Abstract: Virtually all blood pumps contain some kind of rubbing, sliding, closely moving machinery surfaces that are exposed to the blood being pumped. These valves, internal bearings, magnetic bearing position sensors, and shaft seals cause most of the problems with blood pumps. The original teaspoon pump design prevented the rubbing, sliding machinery surfaces from contacting the blood. However, the hydraulic efficiency was low because the blood was able to "slip around" the rotating impeller so that the blood itself never rotated fast enough to develop adequate pressure. An improved teaspoon blood pump has been designed and tested and has shown acceptable hydraulic performance and low hemolysis potential. The new pump uses a nonrotating "swinging" hose as the pump impeller. The fluid enters the pump through the center of the swinging hose; therefore, there can be no fluid slip between the revolving blood and the revolving impeller. The new pump uses an impeller that is comparable to a flexible garden hose. If the free end of the hose were swung around in a circle like half of a jump rope, the fluid inside the hose would rotate and develop pressure even though the hose impeller itself did not "rotate"; therefore, no rotating shaft seal or internal bearings are required.  相似文献   

13.
Background : Our objective was to determine whether administration of propranolol or verapamil modifies the hemodynamic adaptation to continuous positive-pressure ventilation (CPPV), in particular the regional distribution of cardiac output (CO).
Methods : General hemodynamics and regional blood flows assessed by microsphere technique (15 (μm) were recorded in 16 anesthetized pigs during spontaneous breathing (SB) and CPPV with 8 cm H2O end-expiratory pressure (CPPV8) before and after intravenous administration of propranolol (0.3 mg · kg−1 followed by 0.15 mg · kg−1 · h−1, n=8) or verapamil (0.1 mg · kg−1 followed by 0.3 mg · kg−1 · h−1, n=8).
Results : CPPV8 depressed CO by 25% without shifts in its relative distribution with the exception of a noteworthy increase in adrenal perfusion. Propranolol increased arterial blood pressure, and due to a fall in heart rate, CO dropped by 25%. The kidneys and, to a lesser extent, the splanchic region and central nervous system received increased fractions of the remaining CO at the expense of skeletal muscle flow. Similar patterns were seen during SB and CPPV8 such that the combination of propranolol and CPPV8 depressed CO by 50%. The circulatory effects of verapamil were less evident but myocardial perfusion tended to increase.
Conclusions : The combination of propranolol or verapamil with CPPV does not result in any specific hemodynamic interaction in anesthetized pigs, except that the combined effect of propranolol and CPPV may severely reduce CO.  相似文献   

14.
Background : Inhibitory effects of volatile anaesthetics on platelet aggregation have been demonstrated in several studies. However, the influence of volatile anaesthetics on intracoronary platelet adhesion has not been elucidated so far.
Methods : Isolated hearts of guinea pigs were perfused with buffer in the absence or presence of volatile anaesthetics (0.5 and 1 MAC) at constant coronary flow rates of 5 ml/min for 25 min, then 1 ml/min for 30 min and again 5 ml/min for 10 min. Before, during and after low-flow perfusion, a bolus of human platelets was applied into the coronary system. To simulate thrombogenic conditions, 0.3 U/ml human thrombin was infused during low-flow perfusion and reperfusion. The number of platelets sequestered to the endothelium was calculated from the difference between coronary in- and output of platelets. The myocardial production of lactate and consumption of pyruvate and coronary perfusion pressure were also determined.
Results : At a flow rate of 5 ml/min only about 3% of the applied platelets did not emerge from the coronary system, in any group. In contrast, 13.1±1.2% (mean±SEM) of infused platelets became adherent in low-flow perfusion in the control group without anaesthetic. The adherence was reduced with each 1 MAC isoflurane (to 6.2±1.2%), sevoflurane (to 4.4±0.9%) or halothane (to 3.2±1.5%) (each P <0.05 vs. control). Volatile anaesthetic, 0.5 MAC, did not inhibit platelet adhesion to a statistically significant extent in any case. Perfusion pressure and metabolic parameters were not statistically different between the control and the hearts exposed to anaesthetics.
Conclusion : Volatile anaesthetics in a concentration of 1 MAC can reduce the adhesion of platelets in the coronary system under reduced flow conditions. This action does not arise from vasodilation or inhibition of ischaemic stress.  相似文献   

15.
Abstract: A variety of protein-bound or hydrophobic substances, accumulating as a result of pathologic conditions such as exogenous or endogenous intoxications, are removed poorly by conventional detoxification methods because of low accessibility (hemodialysis), insufficient adsorption capabilities (hemosorption), low efficiency (peritoneal dialysis), or economic limitations (high-volume plasmapheresis). Combining advantages of existing methods with microspheric technology, a module-based system was designed. Major operating parameters of the latter can be modified to allow for adjustment to individual clinical situations. An extracorporeal blood circuit including a plasmafilter is combined with a secondary high-velocity plasma circuit driven by a centrifugal pump. Different microspheric adsorbers can be combined in one circuit or applied in sequence. Thus, a prolonged treatment can be tailored using specially designed selective adsorber materials. Comparing this system with existing methods (high-flux hemodialysis, molecular adsorbent recycling system), results from our in vitro studies and animal experiments demonstrate the superior efficiency of substance removal.  相似文献   

16.
Background: Halothane inhibits in vitro and in vivo activity of cytochrome P-450 (CYP) 2E1. There are several fluorinated volatile anaesthetics besides halothane, and most of them are defluorinated by CYP2E1. It is unclear whether other fluorinated anaesthetics inhibit the in vivo activity of CYP2E1.
Methods: We compared the inhibitory effects of therapeutic concentrations of four inhalational anaesthetics, halothane, enflurane, isoflurane, and sevoflurane, on chlorzoxazone metabolism in rabbits receiving artificial ventilation.
Results: All four inhalational anaesthetics decreased arterial blood pressure and increased plasma chlorzoxazone concentration. However, no significant differences in the plasma chlorzoxazone concentration were found between the four anaesthetics. The estimated chlorzoxazone clearance increased after beginning inhalation with all four agents, but no significant difference in clearance was noted between agents.
Conclusions: At therapeutic concentrations, the in vivo inhibitory effect on chlorzoxazone metabolism was similar for all four inhalational anaesthetics examined, even though their chemical characteristics and extent of hepatic metabolism differ considerably.  相似文献   

17.
Abstract Immunoadsorption (1A) therapy with tryptophan (TR-350) or phenylalanine (PH-350) adsorbents has been used to reduce the concentration of serum antibodies in human lymphocyte antigen (HLA)-immunized patients. Other forms of plasma purification have been reported to reduce the level of fibrinogen, which affects the blood properties. In this study we investigated the effects of IA therapy using both adsorbents on plasma fibrinogen and immunoglobulins G and M in 13 patients (8 patients were treated with TR-350, and 5 patients were treated with PH-350). During each session 1 plasma volume (2.8 ± 0.4 L of plasma) was processed through the immunocolumn and then returned to the patient together with the blood cells. Compared with the pretreatment values, the plasma fibrinogen, IgG, and IgM concentrations were significantly reduced after IA therapy (p < 0.01 for TR-350; p < 0.04 for PH-350). There was a positive correlation between the degree of reduction of plasma proteins and the number of IA treatments given. A nonpara-metric test (Wilcoxon's signed-rank test or the Mann-Whitney test) was used for statistical analysis. We conclude from our study that IA therapy effectively lowers the plasma levels of fibrinogen, IgG, and IgM and thus can be considered a valuable alternative to other blood purification methods.  相似文献   

18.
19.
Abstract: Photopheresis is a technique in which peripheral blood mononuclear cells, in the presence of a photoacti-vatable compound, are exposed extracorporeally to ultraviolet A light and reinfused, inducing a host autoregula-tory immune response. Experimental work and ongoing clinical studies are helping to define the role of this novel, safe, and non-toxic immunomodulating technology in the field of transplantation.  相似文献   

20.
Background: Obesity is increasing globallly, including in the formerly "Eastern Bloc" countries. Methods: A survey was made of obesity and bariatric surgery. Results: In the 8 East and Central European countries studied, with total population 300 million, roughly 43% of the population was overweight (BMI 25-30), 23% obese (BMI > 30), with about 15 million people morbidly obese (BMI > 40). From 0-10 morbidly obese individuals/100,000/year undergo bariatric surgery. Conclusion: Most countries were found to provide inadequate treatment for obesity.The majority of the morbidly obese are not treated effectively. However, health-care awareness of obesity and bariatric surgeons are slowly increasing.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号