打包固定法在白癜风表皮移植治疗中的应用

目的:观察打包固定法加碱性成纤维细胞生长因子(bFGF)在白癜风自体表皮移植治疗中应用的效果。方法:15例活动部位的白癜风患者(治疗组)采用自体表皮移植打包法固定加碱性成纤维细胞生长因子,与未加碱性成纤维细胞生长因子的创可贴固定的15例表皮移植患者(对照组)比较疗效。结果:治疗组疗效高于对照组,比较有显著性意义(P<0.01)。结论:打包固定加碱性成纤维细胞生长因子有助于提高活动部位白癜风的自体表皮移植疗效。     

The Fate of Diced Cartilage Grafts of Traumatized Versus Nontraumatized Origin

Background Diced cartilage grafts have long been used in rhinoplasty. Along with their various reported advantages, they also have some disadvantages. The irregular resorption rate of the engrafted mass is one of the major issues noted with diced cartilage grafts. An explanation for the unpredictable resorption rate has not yet been elucidated. This study aimed to determine the role of traumatized versus nontraumatized cartilage as the source of diced cartilage grafts. Methods This study included the noses of 32 patients (19 traumatized noses and 13 nontraumatized noses) who underwent surgery using Surgicel-wrapped diced cartilage grafts. Results The most remarkable result noted in this study was that a Surgicel-wrapped diced cartilage graft, if prepared from traumatized cartilage (in 19 patients), failed to maintain a stable long-term volume. Partial volume loss was noted in 100% of these patients. However, the rate of this partial resorption was different for every individual and could not be predicted. The highest retention of cartilage graft volume was seen primarily over the nasal osseocartilaginous junction. In nontraumatized patients, however, the engrafted mass maintained long-term volume stability. These patients evidenced no visible external irregularities, and only a few very slight imperfections were noted with finger palpation. Conclusion Diced cartilage of traumatic origin is not recommended for any purpose. In such cases, the authors’ engrafting algorithm consists of autobone or ear conchal cartilage grafts for dorsal augmentation, columellar struts from the nontraumatized part of the septum, and spreader grafts from the nontraumatized part of the septum or ear concha.     

A Simple Dressing Technique Following Dermofasciectomy and Full Thickness Skin Grafting of the Fingers in the Treatment of Severe Dupuytren’s Contracture

Dupuytren’s disease with severe finger contractures and recurrent contractures following previous surgery often have extensive skin involvement. In these severe cases, excision of the diseased chord along with the involved skin is a good option to reduce the risk of recurrance. The resulting skin defect can be covered with a full thickness skin graft (FTSG) or a cross finger flap. Cross finger flaps have donor finger morbidity and hence a full thickness graft is usually preferred. The FTSG extending to the midlateral margins on both sides of the finger reduces the risk of joint contracture due to graft shrinkage. Once the FTSG is sutured in place, the standard practice is to compress and secure the graft to its recipient bed with a tie-over dressing and this can be time consuming. We present a simple dressing technique to secure the FTSG without the need for a tie-over dressing.     

‘HYPAFIX’: NEW TECHNIQUE OF SKIN GRAFT FIXATION

A new technique for split skin graft fixation using ‘hypafixy’ adhesive dressing is described. Ninety-three patients with bums involving < 20% body surface area grafted at the adelaide children's hospital during february 1985-may 1987 were reviewed. Comparisons were made between the results of standard fixation and hypafix methods. The hypafix technique showed itself to be simple and reliable, affording considerable versatility and allowing early mobilisation without splintage or sedation. The adhesive is readily loosened with ungvita ointment allowing the dressing to be removed painlessly. In this study its use has shortened both the length of inpalient stay and outpatient follow-up in the scar management programme.     

In-situ characterization of the bacterial biofilm associated with Xeroform™ and Kaltostat™ dressings and evaluation of their effectiveness on thin skin engraftment donor sites in burn patients

Biofilm forms when bacteria surrounded by an extracellular matrix aggregate on a surface. It can develop on many surfaces, including wound dressings; this can be particularly nefarious for burn patients undergoing skin grafting (autograft) for burn wound coverage as they often suffer from compromised immune system function. Autograft donor sites are particularly vulnerable to biofilm formation; as such, timely healing of these sites is essential. Our aim was to apply scanning electron microscopy to compare the efficacy of two types of wound dressings in preventing the formation of bacterial biofilm on burn patient skin graft donor sites. One dressing contained bismuth tribromophenate at a concentration of 3% which confers it bacteriostatic properties (Xeroform?). The other was an absorptive alginate calcium sodium dressing (Kaltostat?). Samples of each wound dressing, which were in contact with the skin graft donor site, were prepared for analysis under the scanning electron microscope (SEM) using an original method developed by our research group that aims to maintain the integrity of the biofilm microstructure. Samples prepared by this method were then analyzed using SEM, which allowed the characterization of biofilm and the evaluation of bacterial density on the studied dressing samples. To this day, this imaging technique has been rarely employed for dressing analysis and this is the first time that it is employed for in situ biofilm visualization for this particular application.     

Laser cartilage reshaping in an in vivo rabbit model using a 1.54 microm Er:Glass laser

BACKGROUND AND OBJECTIVES: The potential applications for facial laser cartilage reshaping (LCR) have generated increasing clinical interest. This study aimed to evaluate in vivo LCR of the rabbit ear using a 1.54 micro m Er:Glass laser in combination with contact cooling. STUDY DESIGN/MATERIALS AND METHODS: LCR was performed in vivo on 12 rabbit ears using a 1.54 micro m Er:Glass laser (Aramis, Quantel Medical, Clermont Ferrand, France) connected to a 4 mm chilled (+5 degrees C) handpiece placed in contact to the skin. Ear curvature was predetermined using a perforated cylindrical guide also used to standardize laser beam delivery. The treatment consisted of 15 spots (3 millisecond, 7 pulses, 12 J/cm(2), 2 Hz, 84/cm(2) cumulative fluence) applied on 10 contiguous parallel rows along the ear. After irradiation, the aluminum jig was replaced by a holder (10 mm diameter plastic tube) maintaining the curvature. This holder was secured with sutures and covered by an adhesive gauze bandage dressing to keep new form during 7 days. In order to assess thermal damage, biopsies were taken on irradiated areas and 1 week, 3 weeks and 6 weeks and studied using haematoxylin-erythrosin-safran (HES) and orcein staining and PCNA to detect cells in cycle. RESULTS: Using the laser with the parameters given above, no immediate visible effects were observed on the skin (no swelling, no bleaching). There were also no late visible side effects like crusting, or blistering. The laser treatment produced changes in the shape of every ear after the dressing was removed. A slight tendency to recover its initial shape was observed for each ear. However, the curvature was stabilized after 10 days and the average shape retention was 64+/-4% at 6 weeks, with a curvature radius of 7.25+/-0.75 mm, instead of 5 mm initially. Histological examination of the laser irradiated side at 1 week showed an intact epidermis. A reduced inflammation process was seen in the dermis. A modification of half of the layer of cartilage was observed at the opposite side where the laser irradiation was applied and proliferative cells were detected inside. At 3 weeks, an important chondroblastic proliferation was observed around the area of contracted cartilage. At 6 weeks, significant thickening of the cartilage layer was observed (from 300 to 490 micro m) and new chondrocytes were clearly seen. CONCLUSIONS: Rabbit ear cartilage can be reshaped with an Er:Glass laser. This technique could offer exciting possibilities that may help patients whose cartilage-lined joints have been affected by disease or trauma. This technique could be certainly utilized to correct alar cartilage deformities and septum deviation of cleft lips.     

Extramucosal Rhinoplasty with Videoscopic Assistance

Extramucosal approach provides for earlier results, less edema and echymoses. The final inspection using the magnificence of the videoscopic camera makes visible small irregularities at the cut edge of the nasal bones, upper portion of the lateral cartilage, and dorsal aspect of the septal cartilage. The removal of both cartilaginous and bony spicules provides improvements in the quality of the results. Videoscopic assistance in association with extramucosal approach is very helpful to achieve an aesthetic and functional result in rhinoplasty.     

筛网状全厚皮片移植联合封闭负压引流治疗四肢皮肤缺损

目的探讨全厚皮片延期移植联合封闭负压引流(VSD)治疗四肢皮肤缺损的临床效果。方法对38例外伤性四肢皮肤缺损患者行清创+VSD术,待缺损区创面肉芽新鲜时,行筛网状全厚皮片移植并用VSD敷料覆盖,持续负压吸引,3～5 d后去除VSD敷料。结果 38例均获随访,时间3个月～2年。35例皮片一次全部成活,3例皮片边缘少许坏死伴渗液,经换药治愈;植皮区外观满意,弹性好,无瘢痕挛缩;取皮区留有线形瘢痕,无瘢痕增生、疼痛等。结论筛网状全厚皮片联合VSD延期植皮修复四肢皮肤缺损成活率高,外观满意,疗效好。     

Otoplasty with an unusual cartilage scoring approach

Background: An ideal otoplasty procedure should minimise the possible risk of severe complications of otoplasty and provide a good aesthetic outcome; however, there is no standart technique to be applied to all types of auricular deformities in different populations. The aim of this study was to present an otoplasty technique with posterior approach in which small incomplete cartilage incisions and suture fixations were used to form the auricle without a need for anterior skin incision and dissection. Methods: This study involved 42 patients who had bilateral prominent ears with unfurled antihelixes associated with or without conchal excess. The otoplasty procedure mainly consisted of a posterior skin excision, incomplete cartilage incisions in the shape of greater mark, “>”, mattress sutures of the posterior cartilage, earlobe correction, and conchal reduction if necessary. Results: This otoplasty technique consists of easy, simple, and rapid surgical steps without a need for anterior skin dissection and cartilage scoring, so it has a low rate of complications in both early and late postoperative periods. Follow-up time of patients ranged from 1–9 years, with an average of 3 years. No major complications such as haematomas, chondritis, wound infection, skin necrosis, asymmetry, recurrence, hypertrophic scars, granulomas, or irregularities developed in the early and late postoperative periods. A good symmetry and natural appearence were achieved with this otoplasty technique. Patients were satisfied with the results. All over complication rate in the postoperative period was 7%. Conclusion: The presented technique overcomes the drawbacks of anterior skin dissection and anterior scoring, and minimises the risk of severe complications such as anterior skin necrosis, cartilage necrosis or destruction, and ear irregularities. It also reduces the operation time, swelling, bruising, and possibility of suture extrusion and recurrence.     

Syringe liposculpture: A two-year experience

Syringe liposcupture is a method that combines two relatively new techniques of plastic surgery: syringe liposuction and fat grafting. We can reshape the face and the body by removing localized fat deposits and reinjecting this fat where needed. When we do not reinject, we call the technique reduction liposculpture. In 1989 we introduced a new technique—superficial syringe liposculpture—to treat patients with flaccid skin, superficial irregularities or depressions, cellulite, and liposuction sequelae. The technique combines syringe liposculpture, superficial liposuction, and our method of treating skin irregularities by breaking the fibrous adherences and injecting fat superficially.     

Use of Vicryl mesh in prevention of postrhinoplasty dorsal irregularities

Small, palpable, and visible dorsal irregularities may occur in as many as 5 to 10% of rhinoplasties. They can be unacceptable to both the surgeon and patient and can lead to unwanted secondary surgery. In 88 patients treated over an 18-month period, Vicryl mesh implants consisting of one to three layers of Vicryl were placed under direct vision over the dorsal cartilage; care was taken to avoid disturbing any cartilage implants. Early results were impressive; there was an absence of palpable dorsal irregularities. This report describes the surgical technique and impressions during the 18-month period.     

Hyaline cartilage degenerates after autologous osteochondral transplantation.

OBJECTIVES: Autologous osteochondral grafting is a well-established clinical procedure to treat focal cartilage defects in patients, although basic research on this topic remains sparse. The aim of the current study was to evaluate (1) histological changes of transplanted hyaline cartilage of osteochondral grafts and (2) the tissue that connects the transplanted cartilage with the adjacent cartilage in a sheep model. METHOD: Both knee joints of four sheep were opened surgically and osteochondral grafts were harvested and simultaneously transplanted to the contralateral femoral condyle. The animals were sacrificed after three months and the received knee joints were evaluated histologically. RESULTS: Histological evaluation showed a complete ingrowth of the osseous part of the osteochondral grafts. A healing or ingrowth at the level of the cartilage could not be observed. Histological evaluation of the transplanted grafts according to Mankin revealed significantly more and more severe signs of degeneration than the adjacent cartilage, such as cloning of chondrocytes and irregularities of the articular surface. CONCLUSION: We found no connecting tissue between the transplanted and the adjacent cartilage and histological signs of degeneration of the transplanted hyaline cartilage. In the light of these findings, long-term results of autologous osteochondral grafts in human beings have to be followed critically.     

Autologous Cartilage Graft Rhinoplasties

Background: The authors report their experience with autologous graft rhinoplasties. Methods: Data were collected retrospectively, with selection of only autologous grafts from 2,000 rhinoplasties performed at the Plastic and Reconstructive Department of the University of Rome Tor Vergata. Results: A total of 62 patients from January 1995 to January 2005 were selected. Most of the patients were treated with the “open tip” technique, whereas 9.7% had a classic endonasal approach. Follow-up evaluation was performed with outpatient visits at 2 and 6 weeks, then at 3, 6, and 12 months. Good aesthetic results were obtained for 93.5% of the patients, and 83.7% had complete satisfaction. Conclusion: Autologuos cartilage graft rhinoplasty is an affordable technique easy to learn that widens possibilities of interventions for nasal pyramid reconstruction.     

VSD治疗手部感染性创面的疗效分析

目的探讨应用负压封闭引流技术(Vacuum Sealing Drainage,VSD)治疗手部感染性创面的临床效果。方法回顾性分析2014年12月-2019年3月收治的皮肤缺损伴感染患者40例。随机分为VSD治疗组(A组)20例,常规换药治疗组(B组)20例。A组给予手术清创,负压引流材料覆盖创面,并给予庆大霉素持续冲洗。B组给予手术清创后,每日换药。两组肉芽组织新鲜后给予植皮治疗。回顾性分析两组平均住院时间、创面愈合时间、创面评分、疼痛程度评分、抗菌药物使用时间、换药次数。结果A组住院时间、创面愈合时间、创面评分、疼痛程度评分、抗菌药物使用时间、换药次数均少于B组,A组疼痛评分低于B组。两组差异有统计学意义(P<0.01)。结论应用VSD治疗手部感染性创面可促进肉芽组织生长,加速创面愈合,减少患者住院时间及抗菌药物使用,减轻患者的痛苦,临床效果良好,值得推广。     

A prospective randomized controlled trial comparing negative pressure dressing and conventional dressing methods on split-thickness skin grafts in burned patients

Introduction

Split-thickness skin grafting (SSG) is a technique used extensively in the care of burn patients and is fraught with suboptimal graft take when there is a less-than-ideal graft bed and/or grafting conditions. The technique of Negative Pressure Dressing (NPD), initially used for better wound healing has been tried on skin-grafts and has shown to increase the graft take rates. However, comparative studies between the conventional dressing and vacuum assisted closure on skin grafts in burn patients are unavailable. The present study was undertaken to find out if NPD improves graft take as compared to conventional dressing in burns patients.

Materials and methods

Consecutive burn patients undergoing split-skin grafting were randomized to receive either a conventional dressing consisting of Vaseline gauze and cotton pads or to have a NPD of 80 mm Hg for four days over the freshly laid SSG. The results in terms of amount of graft take, duration of dressings for the grafted area and the cost of treatment of wound were compared between the two groups.

Results

A total of 40 split-skin grafts were put on 30 patients. The grafted wounds included acute and chronic burns wounds and surgically created raw areas during burn reconstruction. Twenty-one of them received NPD and 19 served as controls. Patient profiles and average size of the grafts were comparable between the two groups. The vacuum closure assembly was well tolerated by all patients. Final graft take at nine days in the study group ranged from 90 to 100 per cent with an average of 96.7 per cent (SD: 3.55). The control group showed a graft take ranging between 70 and 100 percent with an average graft take of 87.5 percent (SD: 8.73). Mean duration of continued dressings on the grafted area was 8 days in cases (SD: 1.48) and 11 days in controls (SD: 2.2) after surgery. Each of these differences was found to be statistically significant (p < 0.001).

Conclusion

Negative pressure dressing improves graft take in burns patients and can particularly be considered when wound bed and grafting conditions seem less-than-ideal. The negative pressure can also be effectively assembled using locally available materials thus significantly reducing the cost of treatment.     

Simplified Negative Pressure Wound Therapy in Pediatric Hand Wounds

Negative pressure wound therapy (NPWT) is commonly used as a bolster for skin grafts. The technique offers the benefit of negative pressure as well as reduced dressing changes. Skin grafting of the hand provides a unique challenge, and currently, the only commercially available NPWT hand dressings are adult-sized, precluding their use in small children. We present our custom NPWT “mitten” technique for use with skin grafts on the pediatric hand.     

Negative‐pressure wound dressings to secure split‐thickness skin grafts in the perineum

Several researches have shown that negative‐pressure wound dressings can secure split‐thickness skin grafts and improve graft survival. However, in anatomically difficult body regions such as the perineum it is questionable whether these dressings have similar beneficial effects. In this study, we evaluated the effects of negative‐pressure wound dressings on split‐thickness skin grafts in the perineum by comparing wound healing rate and complication rate with that of tie‐over dressings. A retrospective chart review was performed for the patients who underwent a split‐thickness skin graft to reconstruct perineal skin defects between January 2007 and December 2011. After grafting, the surgeon selected patients to receive either a negative‐pressure dressing or a tie‐over dressing. In both groups, the initial dressing was left unchanged for 5 days, then changed to conventional wet gauze dressing. Graft success was assessed 2 weeks after surgery by a single clinician. A total of 26 patients were included in this study. The mean age was 56·6 years and the mean wound size was 273·1 cm². Among them 14 received negative‐pressure dressings and 12 received tie‐over dressings. Negative‐pressure dressing group had higher graft taken rate (P = 0·036) and took shorter time to complete healing (P = 0·01) than tie‐over dressing group. The patients with negative‐pressure dressings had a higher rate of graft success and shorter time to complete healing, which has statistical significance. Negative‐pressure wound dressing can be a good option for effective management of skin grafts in the perineum.     

Comparison of the efficacy and safety of povidone‐iodine foam dressing (Betafoam), hydrocellular foam dressing (Allevyn), and petrolatum gauze for split‐thickness skin graft donor site dressing

We evaluated the efficacy and safety of a povidone‐iodine (PVP‐I) foam dressing (Betafoam) for donor site dressing versus a hydrocellular foam dressing (Allevyn) and petrolatum gauze. This prospective Phase 4 study was conducted between March 2016 and April 2017 at eight sites in Korea. A total of 106 consenting patients (aged ≥ 19 years, scheduled for split‐thickness skin graft) were randomised 1:1:1 to PVP‐I foam, hydrocellular, or petrolatum gauze dressings for up to 28 days after donor site collection. We assessed time to complete epithelialisation, proportion with complete epithelialisation at Day 14, and wound infection. Epithelialisation time was the shortest with PVP‐I foam dressing (12.74 ± 3.51 days) versus hydrocellular foam dressing (16.61 ± 4.45 days; P = 0.0003) and petrolatum gauze (15.06 ± 4.26 days, P = 0.0205). At Day 14, 83.87% of PVP‐I foam dressing donor sites had complete epithelialisation, versus 36.36% of hydrocellular foam dressing donor sites (P = 0.0001) and 55.88% of petrolatum gauze donor sites (P = 0.0146). There were no wound infections. Incidence rates of adverse events were comparable across groups (P = 0.1940). PVP‐I foam dressing required less time to complete epithelialisation and had a good safety profile.     

A new approach for the refinement of the very broad nasal tip.

Refining the very broad nasal tip has been difficult to achieve with a single technique. Operations described often violate the domes and may result in visible irregularities and collapse of the ala. Adequate narrowing can be obtained by decreasing the angle between medial and lateral crura of the ala cartilage at the dome and decreasing the overall length of the ala cartilage. This narrowing is achieved predictably by removing a small segment at the lateral most part of the ala cartilage. Scoring of the dome can also be done. We describe the technique in a large series of patients, all of whom have retained their results without undesirable problems.