Cem-Ostetic修复牙种植体颊侧骨缺损的初步实验观察

目的:探讨Cem-Ostetic(骨速刚)修复牙种植体颊侧骨缺损的可行性.方法:拔除3只犬双侧下颌第二、三、四前磨牙,每侧即刻植入3颗种植体,并在种植体颊侧骨壁制造3 mm×3 mm×2 mm缺损.暴露种植体,由近中向远中依次植入Bio-Oss骨粉、磷酸钙骨水泥、Cem-Ostetic骨浆.种植术后4、8、12周各处死...     

骨收集器收集自体骨充填种植体周围骨缺损的实验研究

目的:研究利用植入钻孔时骨收集器收集的骨碎末修复种植体周围骨缺损的可行性。方法:以5只家犬双侧下颌骨为实验对象,拔除双侧各3颗前磨牙3月后,行种植术,每侧植入4颗种植钉。种植时人为造成种植体颊侧2mm×2mm大小骨缺损,在同体同侧的4个骨缺损中的3个,分别植入自体骨屑、Bio-Oss骨粉、二者1∶1混合骨末,剩余1处不植入任何材料作为对照。9周后进行各组间比较。结果:植入的自体骨碎屑修复了一定的骨缺损,自体骨混合Bio-Oss组骨缺损恢复最佳。各组与对照组比较均存在统计学差异。结论:植入钻孔时收集的自体骨碎屑对于修复种植体周围少量的骨缺损,是完全可行的。当供骨量不足时,自体骨碎末可以混合适当的Bio-Oss颗粒植入,效果更为理想。     

PRP及PRP/OAM对种植体周围骨缺损修复的实验研究

目的探讨富血小板血浆(PRP)以及PRP复合骨诱导活性材料(PRP/OAM)对种植体周围骨缺损修复的作用。方法选取成年Beagle犬4只,体质量10~13 kg,每只犬左右两侧下颌第四前磨牙牙位随机分为A组和B组,与B组同侧的第一、二前磨牙牙位作为对照组。A组种植体周围骨缺损区植入PRP/OAM,B组植入PRP/磷酸三钙,对照组植入磷酸三钙。采用能谱分析各组种植体-新骨界面Ca~(2+)含量,同时观察各组种植术后8、16周组织学形态。结果 A组和B组种植术后8、16周时种植体部位骨表面Ca~(2+)含量均明显高于对照组(P<0.05);A组种植术后8、16周时种植体部位骨表面Ca~(2+)含量为(26.01±3.28)%和(44.10±7.11)%,明显高于B组(P<0.05);种植术后8周,A组新骨与种植体形成区段性骨结合,B组种植体边缘可有新骨形成,对照组种植体边缘为纤维性界面;种植术后16周,A组和B组新骨与种植体形成骨整合,对照组仅为纤维性结合。结论 PRP及PRP/OAM在种植体周围骨缺损修复中,能有效促进骨缺损修复。     

使用金骨威人工骨粉作为植骨材料的临床观察

目的:评价金骨威骨粉用于骨增量手术植骨的可行性,对临床应用提出指导意见.方法:通过在临床患者中使用金骨威骨粉混合自体骨或Bio-Oss骨粉作为植骨材料,同期或延期植入CDIC种植体后对其临床效果进行随访观察.结果:共计病例6例,8个植骨区,植入种植体16枚,完成修复16枚.病例随访3.33～4.75年,87.5%(14/16)的种植体达到临床成功标准.结论:在骨增量手术中使用金骨威同自体骨粉混合或同Bio-Oss骨粉混合使用作为植骨材料是可行的.     

Bio-Oss骨粉联合富血小板纤维蛋白在牙槽骨缺损种植引导骨再生后的骨量变化

目的：探讨Bio-Oss骨粉联合富血小板纤维蛋白在牙槽骨缺损种植引导骨再生后骨量的变化。方法：选择106例单颗前牙缺失伴唇侧骨缺损患者,进行种植体种植同期引导骨再生。按随机数字表法随机分为2组,实验组（53例）采用Bio-Oss骨粉联合富血小板纤维蛋白+生物膜引导骨再生,对照组（53例）采用Bio-Oss骨粉联合生物膜引导骨再生。评价2组种植成功率、术后并发症率、种植体唇侧骨壁厚度、骨缺损再生情况。采用SPSS 25.0软件包对数据进行统计学分析。结果：2组种植体种植成功率差异无统计学意义（96.23%:88.68%,P>0.05）。种植后12个月,实验组种植体唇侧骨壁厚度显著大于对照组[（2.72±0.43） mm:（2.51±0.36） mm,P<0.05],不同位点种植体唇侧骨壁厚度大于对照组（P<0.05）,出血指数[（0.32±0.02）:（0.42±0.03）]、探诊深度[（3.31±0.69） mm:（4.32±0.95） mm]、附着丧失[（3.06±0.52） mm:（5.24±1.35） mm]均显著小于对照组（P<0.05）,植骨高度[（2.61±0.52） mm:（2.31±0.35） mm]、成骨高度[（2.59±0.32） mm:（2.01±0.16） mm] 显著大于对照组（P<0.05）。2组患者并发症发生率相比差异无统计学意义（1.89%:5.66%, P>0.05）。结论：Bio-Oss骨粉联合富血小板纤维蛋白可减少骨缺损种植引导骨再生后骨量丢失,促进骨缺损再生。     

脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究

目的 比较骨缺损区植入脱矿牙本质基质和脱细胞牙本质基质的成骨效果。方法 制备脱矿牙本质基质和脱细胞牙本质基质。将24只SPF级SD雄性大鼠随机分为脱矿组（A组）、脱细胞组（B组）、Bio-Oss骨粉组（C组）、空白组（D组）,每组6只大鼠,在麻醉条件下制备双侧股骨骨缺损。A、B、C组大鼠分别在骨缺损区植入脱矿牙本质基质、脱细胞牙本质基质、Bio-Oss骨粉,D组大鼠不植入任何材料。术后4周和8周,每组各随机处死3只大鼠。大体观察骨缺损区愈合情况,血清学检测成骨指标骨形态发生蛋白（BMP）-2及碱性磷酸酶（ALP）浓度,影像学观察骨缺损区高密度灰色区（代表骨愈合）分布情况,组织形态学观察新骨形成情况,计算新骨形成率。结果 术后4周和8周,大体观察见A组成骨能力较其他组活跃,血清学检测A组BMP-2及ALP浓度均高于其他组,差异有统计学意义（P<0.05）。8周时,影像学观察可见A组骨缺损区高密度灰色区分布均匀,组织形态学观察见A组排列规则的骨基质。A组4、8周时的新骨形成率分别为28.51%±0.55%、32.57%±2.28%,均高于其他组,差异有统计学意义（P<0.05）...     

自体骨碎末与复合移植修复种植体周围骨缺损的比较研究

目的：研究自体骨碎末与混入Bio-Oss复合移植修复种植体周围骨缺损的效果。方法：9周及16周后对种植体周围骨缺损处植入自体骨末、自体骨末与Bio-Oss二者1：1混合骨末及未植骨进行比较。结果：植入的自体骨碎末恢复了一定的骨缺损，混合Bio—Oss组恢复最佳。各组16周时骨整合均优于9周。结论：植入钻孔时收集的自体骨碎末对于恢复种植体周围少量的骨缺损，是完全可行的。自体骨碎末作为供骨量不足时，可以混合适当的Bio—Oss颗粒行复合移植，效果更为理想。     

种植体＋bFGF基因修饰骨髓基质细胞复合Bio-Oss胶原修复犬颌骨缺损的实验研究

目的:(1)观察经bFGF基因转染的犬骨髓基质细胞(BMSC)复合多孔矿化Bio-Oss胶原骨修复下颌骨极限骨缺损的效果.(2)观察骨融合种植体与骨髓来源的成骨细胞及多孔矿化Bio-Oss胶原支架复合体,植入体内后新骨的形成及与种植体的愈合情况.方法:用脂质体转染技术将bFGF基因转入犬BMSC,将转染和未转染细胞分别与Bio-Oss及凝胶复合.杂种犬8只,按植人物不同分为2组:(1)种植体+犬BMSC+凝胶+Bio-Oss胶原材料组;(2)种植体+bFGF基因转染犬BMSC+凝胶+Bio-Oss胶原材料组,修复犬下颌骨极限骨缺损.于术后24周取材,分别行大体、放射线、组织学观察骨缺损的修复情况.结果:(1)种植体+犬BMSC+凝胶+Bio-Oss骨胶原组,基本上由新生骨组织所修复,但骨组织密度稍不均匀,种植体与周嗣骨组织基本上形成骨结合.(2)种植体+bFGF基因转染犬BMSC+凝胶+Bio-Oss胶原材料组:骨缺损区基本由新生成熟的骨组织所修复,骨小梁形成,与种植体骨结合良好.结论:Bio-Oss骨胶原为载体的自体骨髓基质细胞移植能有效地修复骨缺损,是骨组织工程良好的支架材料,并且种植体与组织工程骨能够形成良好的骨结合.     

两种人工骨材料在修复即刻种植体周骨缺损成骨效果的实验研究

目的:比较β-磷酸三钙(β-tricalcium phosphate,β-TCP)、珊瑚转化型羟基磷灰石生物陶瓷(coral-hydroxyapatite,CHA)两种人工骨材料在引导骨再生中成骨质量的差别,为临床应用提供指导.方法:拔除 Begeal 犬的下颌双测第二三四前磨牙,并植入种植体,在种植体颈部颊侧植被缺损,并以不同人工骨材料修复.结果:种植体均未脱落,缺损区粘膜愈合良好. A 组:1、4个月时新骨生成率分别为23.00%±0.82%、58.00%±1.83%,种植体颈缘到颊侧骨缺损底部的距离为1.05±0.13mm、1.09±0.10mm. B 组:1、4个月时的新骨生成率分别为23.75%±0.96%、57.75%±1.89%,种植体颈缘到颊侧骨缺损底部的距离分别为1.09±0.10mm、1.13±0.10mm. C 组:1 个月和4个月时的新骨生成率分别为4.25%±0.96%、16.50%±1.91%,种植体颈缘到颊侧骨缺损底部的距离分别为4.38±0.17mm、3.75±0.13mm.结论: CHA 及β-TCP 都具有良好的骨引导作用,可用于即刻牙种植骨缺损的修复.     

前牙美学区早期种植联合GBR术后骨量变化的影像学研究

目的利用影像学手段探讨前牙美学区早期种植联合GBR术后16~24周骨量变化。方法 收集 2016年12月至2017年12月就诊的21例(29颗缺失牙)上前牙缺失患者,行软组织愈合的早期种植,植入Straumann骨水平种植体29枚,分别于术后即刻、术后16~24周拍摄锥形束CT(cone beam computed tomography, CBCT),利用CS 3DImagine数字化软件,对种植体平台下方3mm,6mm,9mm唇侧骨板厚度进行测量并对数据进行统计学分析。结果 种植体平台下3mm组术后即刻、术后16~24周唇侧骨厚度分别为(2.97±0.62)mm,(2.49±0.61)mm。种植体平台下6mm组术后即刻、术后16~24周唇侧骨厚度分别为(3.12±0.56)mm,(2.86±0.62)mm。种植体平台下9mm组术后即刻、术后16~24周唇侧骨厚度分别为(2.38±0.56)mm,(2.11±0.54)mm,各位点术后即刻与术后16~24周唇侧骨厚度均有统计学差异( P <0.05)。术后即刻、术后16~24周唇侧骨体积分别为(131.15±27.36)mm 3、(100.57±32.80)mm 3,二者有统计学差异( P <0.05)。唇侧骨体积变化量(30.57±22.89)mm 3,唇侧骨体积变化率(23.08±16.50)%。结论 前牙美学区早期种植联合GBR术后短期内可以达到较好且可靠的效果。     

The combined use of bioresorbable membranes and xenografts or autografts in the treatment of bone defects around implants. A study in beagle dogs

The aim of the present investigation was to test the effect of a bioresorbable membrane supported by xenografts or autografts in regenerating bone into peri-implant defects. In 3 dogs, the mandibular premolars P2, P3, P4 and M1 were extracted bilaterally. After 4 months of healing, 3 standardized bone defects were prepared on each side of the mandible and 1 implant per defect was placed. The 6 sites in each dog were distributed into 4 different treatment groups: 2 sites received a Bio-Gide membrane alone (BG); 2 sites received a Bio-Gide membrane supported by Bio-Oss (BG + BO); 1 site received the Bio-Gide membrane supported by autogenic bone harvested from the prepared defects (BG + Aut); 1 site received neither membrane nor bone graft and served as control (C). The soft tissue flaps were adapted and sutured for primary healing. No adverse events occurred during the experimental period. After 16 weeks, the dogs were sacrificed and histomorphometric examinations on non-decalcified ground sections were carried out. The vertical bone growth amounted to 45% (SD +/- 13%) of the defect height in the BG group, to 78% (SD +/- 29%) in the BG + BO group, to 69% (SD +/- 9%) in the BG + Aut group, and to 22% (SD +/- 10%) in C group. The horizontal bone growth measured 78% (SD +/- 16%) in the BG group, 81% (SD +/- 21%) in the BG + BO group, 82% (SD +/- 12%) in the BG + Aut group, and 46% (SD +/- 21%) in the C group. The vertical height of bone growth in contact with the implant measured 17% (SD +/- 12%) in the BG group, 20% (SD +/- 12%) in the BG + BO group, 17% (SD +/- 7%) in the BG + Aut group, and 12% (SD +/- 8%) in the C group. The surface fraction of the graft in direct bone contact measured 89% (SD +/- 9%) in the BG + BO group and 93% (SD +/- 3%) in the BG + Aut group. It is concluded that the bioresorbable membrane tested enhances bone regeneration, in particular in conjunction with the use of a supporting graft material. In addition, deproteinized bovine bone mineral and autogenic bone grafts appeared to be equally well integrated into regenerating bone. Finally, no additional effects in the bone growth was observed with the autogenous bone in comparison with the hydroxyapatite.     

Effects of different depths of gap on healing of surgically created coronal defects around implants in dogs: a pilot study

BACKGROUND: This study investigated the bone growth pattern in surgically created coronal defects with various depths around implants in dogs. METHODS: Four mongrel dogs were used. All mandibular premolars were extracted under general anesthesia and left to heal for 2 months. After ostectomy, bony defects were prepared in test sites, using a stepped drill with a diameter of 6.3 mm and two depths: 2.5 mm (test sites 1 [T1]) and 5.0 mm (test sites 2 [T2]). In the control sites, the implants were placed after ostectomy without any coronal defects. T1, T2, and control sites were prepared in the right and left sides of the mandible. Six implants, 3.3 mm in diameter and 10 mm in length, were placed in each dog; the implants were submerged completely. Two dogs were sacrificed 8 weeks after surgery, and the other two dogs were sacrificed 12 weeks after surgery. The stability of all implants was measured with a resonance frequency analyzer after placement and after sacrifice. All sites were block-dissected for ground sectioning and histologic examination. RESULTS: After 12 weeks of healing, only T2 were not filled fully with bone. At week 8, the mean bone-to-implant contact (BIC) was 47.7% for control, 43.6% for T1, and 22.2% for T2. At week 12, the control BIC was 56.7% and the 2.5-mm defect had a greater BIC (58.8%). However, in the 5-mm defect, the BIC was 35.1%. At insertion, stability was reduced at sites with a greater defect depth. Similar stability was noted in all specimens after 8 and 12 weeks of healing. CONCLUSION: Bone healing between an implant and marginal bone was compromised at sites with a deeper defect when the width of the bone defect was 1.5 mm.     

The effect of a fibrin glue on the integration of Bio-Oss with bone tissue. A experimental study in labrador dogs

BACKGROUND: Bio-Oss is a deproteinized bovine mineral used in bone augmentation procedures. The particles are often mixed with a protein product (Tisseel) to form a mouldable graft material. AIM: The aim of the present experiment was to study the healing of self-contained bone defects after the placement of Bio-Oss particles alone or mixed with Tisseel in cylindrical defects in the edentulous mandibular ridge of dogs. MATERIAL AND METHODS: In 4 labrador dogs, the 2nd, 3rd and 4th mandibular premolars were extracted bilaterally. 3 months later, 3 cylindrical bone defects, 4 mm in diameter and 8 mm in depth, were produced in the right side of the mandible. Following a crestal incision, full thickness flaps were raised and the bone defects were prepared with a trephine drill. The defects were filled with Bio-Oss (Geistlich Biomaterials, Wolhuser, Switzerland) particles alone or mixed with Tisseel (Immuno AG, Vienna, Austria), or left "untreated". A collagen membrane (Bio-Gide, Geistlich Biomaterials, Wolhuser, Switzerland) was placed to cover all defects and the flaps were sutured. 2 months later, the defect preparation and grafting procedures were repeated in the left side of the mandible. After another month, the animals were sacrificed and biopsies obtained from the defect sites. RESULTS: Bio-Oss-treated defects revealed a higher percentage of contact between graft particles and bone tissue than defects treated with Bio-Oss+ Tisseel (15% and 30% at 1 and 3 months versus 0.4% and 8%, respectively). Further, the volume of connective tissue in the Bio-Oss treated defects decreased from the 1 to the 3 month interval (from 44% to 30%). This soft tissue was replaced with newly formed bone. In the Bio-Oss+ Tisseel treated defects, however, the proportion of connective tissue remained unchanged between 1 and 3 months. CONCLUSION: The adjunct of Tisseel may jeopardize the integration of Bio-Oss particles with bone tissue.     

The effect of bone condensation and crestal preparation on the bone response to implants designed for immediate loading: a histomorphometric study in dogs

PURPOSE: The aim of this study was to evaluate the influence of bone condensation and crestal preparation on the bone response of implants designed to promote osseocompression. MATERIALS AND METHODS: In the first phase, the mandibular premolars of 6 dogs were extracted bilaterally. After 8 weeks, each dog received 8 Xive implants (4 per hemimandible). One hemimandible was randomly assigned to the experimental group and the other to the control group. The implant site was prepared using conventional standard drills. Prior to implant placement the crestal drill was used in the experimental group but not in the control group. After 12 weeks, the animals were sedated and sacrificed. The hemimandibles were removed and prepared for histomorphometric analysis of bone-implant contact (BIC) and bone density of areas adjacent to and further from the implant surface. RESULTS: The mean +/- SD percentages of BIC attained were 71.1% +/- 11.8% and 45.1% +/- 16.1% for the experimental and control groups, respectively. The bone density analysis revealed that in the control group, percentage BIC was a mean of 55.6% +/- 11.3% adjacent to the implant and 50.7% +/- 17.9% distant from the implant. In the experimental group, percentage BIC was a mean of 71.1% +/- 8.6% adjacent to the implant and 55.6 +/- 11.3 distant from the implant. The difference between the experimental and control groups was statistically significant for both parameters, BIC and bone density, in the adjacent areas (P < .0001). CONCLUSION: Crestal preparation is of fundamental importance for this implant system, since it led to better bone response, represented by the improved BIC and bone density.     

An oxidized implant surface may improve bone-to-implant contact in pristine bone and bone defects treated with guided bone regeneration: an experimental study in dogs

BACKGROUND: The aim of the present study was to histometrically evaluate bone healing in the absence of bone defects and in the presence of surgically created bone defects treated by guided bone regeneration at oxidized and turned implant surfaces. METHODS: Three months after dental extractions, standardized buccal dehiscence defects (height: 5 mm; width: 4 mm) were surgically created following implant site preparation in the mandible of 10 dogs. Oxidized-surface implants (OSI) and turned-surface implants (TSI) were inserted bilaterally, and the bone defects were treated by guided bone regeneration. After 3 months of healing, the animals were sacrificed, blocks were dissected, and undecalcified sections were obtained and processed for histometric analysis. The percentage of bone-to-implant contact (BIC) and bone density (BD) was evaluated inside the threads on the buccal (regenerated bone) and lingual sides (pristine bone) of the implants. Data were evaluated using two-way analysis of variance (P <0.05). RESULTS: New bone formation could be observed in OSI and TSI in the region of the defect creation. The BIC values observed in OSI for pristine and regenerated bone were 57.03% +/- 21.86% and 40.86% +/- 22.73%, respectively. TSI showed lower values of BIC in pristine bone (37.39% +/- 23.33%) and regenerated bone (3.52% +/- 4.87%). The differences between OSI and TSI were statistically significant. BD evaluation showed no statistically significant differences between OSI and TSI in pristine and regenerated bone. CONCLUSION: The oxidized implant surface promoted a higher level of BIC than the turned implant surface at pristine and regenerated bone.     

浓缩生长因子促进犬种植体周围骨缺损修复的实验研究

目的:探讨浓缩生长因子(concentrate growth factors,CGF)促进犬种植体周围骨缺损修复的能力。方法:Beagle犬4只,拔除双侧下颌第一前磨牙作为实验牙位。3个月后,待拔牙窝内骨组织基本成熟后植入种植体,其周围制备骨缺损(外侧壁制备深4 mm,颊舌及近远中向各1 mm的环行骨缺损)并植入骨移植材料;随机选取实验动物的左右侧下颌第一前磨牙,分别作为实验组和对照组,实验组植入CGF与磷酸三钙(tricalcium phosphate,TCP)的混合物;对照组植入TCP。8、16周后分别处死动物2只,进行X线、组织学观察,能谱分析种植体-新骨界面钙含量。采用SPSS13.0软件包对数据进行统计学分析。结果:8周及16周时,肉眼及组织学观察发现,实验组修复效果显著优于对照组,种植体-新骨界面钙含量均高于对照组,差异显著(P<0.05)。结论:CGF能够促进犬种植体周围骨缺损的修复,缩短骨整合时间,提高愈合质量。     

Histologic findings in sinus augmentation with autogenous bone chips versus a bovine bone substitute

PURPOSE: The aim of this study was to compare a bovine bone substitute (Bio-Oss) to autogenous bone with respect to its value as a material for sinus augmentation. MATERIALS AND METHODS: In 10 beagle dogs 12 months of age, the 3 maxillary premolars were extracted on both sides. Six weeks later, 2 cavities of predefined size were produced in the region of the nasal cavity. The antral window was 25 mm long and had a vertical extension of 7 mm. Two Frialit-2 implants (3 x 8 mm) were placed in each bone defect (n = 20). Every implant was primarily stable because of fixation in native bone. In each maxilla, 1 bone defect was filled with autogenous bone harvested from the mandible and 1 was filled with Bio-Oss (material selected at random). The animals were sacrificed at 90 and 180 days, and histologic specimens were examined and the results subjected to statistical analysis by the Wilcoxon test for paired observations. RESULTS: No healing problems were observed. Histologically, after 90 days the volume of the augmentation showed a reduction of 14.6 +/- 4.4% within the Bio-Oss group and 3.8 +/- 2.5% in the group with autogenous bone. Bone-implant contact of 52.16 +/- 13.15% in the Bio-Oss group and 60.21 +/- 11.46% in the autogenous bone group was observed. At 180 days, the Bio-Oss group showed bony ingrowth of the substitute, whereas in the autogenous group a differentiation from original bone could no longer be made. The volume reduction was 16.5 +/- 8.67% in the Bio-Oss group and 39.8 +/- 16.14% in the autogenous group. Bone-implant contact of 63.43 +/- 19.56% in the Bio-Oss group and 42.22 +/- 12.80% in the autogenous bone group was measured. DISCUSSION AND CONCLUSION: The results indicated that because of the nonresorptive properties of the bone substitute Bio-Oss, regeneration of the defects is achievable. It was demonstrated that the bone substitute seemed to behave as a permanent implant. The volume of the area augmented by autogenous bone decreased over the observation period.     

钛网+膜联合使用修复种植体周围牙槽骨缺损的实验研究

目的 探讨联合使用钛网+Bio-Gide膜修复比格犬种植体周围牙槽骨缺损的效果。方法 选择3只成年比格犬,拔除双侧下颌P1-M1,牙槽窝愈合6个月。将3只比格犬（共6侧）随机分为3组,A组使用种植体+骨粉+钛网,B组使用种植体+骨粉+Bio-Gide膜,C组使用种植体+骨粉+钛网+Bio-Gide膜。每侧选取3个位点制备种植窝,在颊侧制作4 mm×4 mm×4 mm牙槽骨缺损,植入种植体并分别覆盖钛网或Bio-Gide膜。于术后6个月处死动物并取材,Micro-CT扫描并三维重建。使用SPSS17.0软件包对测量结果进行统计学分析。结果 Micro-CT扫描及三维重建结果显示,C组的骨小梁数目、骨小梁厚度、骨密度等数据显著高于另外两组（P<0.01）,骨小梁分散度显著低于另外两组（P<0.01）。结论 联合使用钛网+Bio-Gide膜,对比格犬种植体周围牙槽骨缺损的修复作用显著优于单纯使用钛网或Bio-Gide膜。     

Healing and osseointegration of submerged microtextured oral implants

The success of dental implants is primarily dependent upon the degree of osseointegration or bone-to-implant contact (BIC), possibly facilitated by a roughened implant surface. This study was performed to histologically evaluate the nature of osseointegration and bone healing of submerged microtextured implants in eight dogs. Three months following tooth extraction in the posterior mandibulae, three microtextured submerged implants were placed in each quadrant. Block biopsies were harvested at 4 and 16 weeks (four dogs each) following surgery, and histologic preparation was performed. Histomorphometric analysis demonstrated that % BIC value increased marginally from 40% at 4 weeks to 48% at 16 weeks, without a statistically significant difference. The first bone-to-implant contact (f-BIC) at 16 weeks was significantly lower than the 4-week f-BIC (0.81 mm vs. 0.56 mm). In conclusion, this study found minimal change in BIC over time (from 4 to 16 weeks) in unloaded microtextured implants, while the mean f-BIC value significantly increased during this same observation period.     

表面梯度涂层纯钛植入体骨结合和骨诱导能力的动物实验研究

目的：观察纯钛植入体表面用低温烧结技术生成羟基磷灰石/生物玻璃( nHA/BG)梯度涂层植入动物体内的骨结合和骨诱导能力。方法：将nHA/BG按比例混合,应用低温烧结技术在圆柱形纯钛植入体表面形成梯度涂层,模拟体液浸泡后产生活性纳米结构。将梯度涂层实验植入体和纯钛对照植入体分别植入12只新西兰兔股骨髁。术后不同时间行X线摄片,切取标本;硬组织切片,用四环素荧光标记观察植入体周围新生骨形成量;苦味酸-品红染色观察植入体-骨界面骨沉积和结合情况。结果：低温烧结和模拟体液浸泡制备的nHA/BG梯度涂层为纳米化多孔粗糙结构,动物实验示,梯度涂层植入体与周围新生骨明显增多,骨整合良好。结论：梯度涂层和模拟体液浸泡制备的植入体涂层有良好的骨结合和骨诱导能力。