儿童唾液成分与龋齿关系的分析

本实验对１０７名４－６岁乳牙儿童取混合唾液检测流速（Ｖ），唾液钙（Ｃａ），磷（Ｐ）唾液总蛋白（ＴＰ），及唾液免疫球蛋白ＩｇＧ，ＩｇＡ的含量进行分析，探讨其与龋齿的关系，结果显示：１，唾液的ＴＰ，ＩｇＧ，ＩｇＡ含量无龋组低于患龋线两组均数之间有显著性差异（Ｐ〈０．０５，Ｐ〈０．０１，Ｐ〈０．０１），２．唾液的Ｖ和Ｃａ，Ｐ含量，无龋组与患龋组两组均数之间无显著性差异（Ｐ〉０．０５），３无龋组快于唾液Ｖ     

复发性口疮（RAU）与唾液免疫球蛋白,补体及溶菌酶活性关系 …

本文对９９例复发性口疮患者，分别在初诊治疗前（发作期）和治疗后３个月未发作时进行唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶含量和活性检测，并以４５名健康学生唾液相应成份作为正常对照。实验结果表明：复发性口疮患者治疗前唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶含量和活性，均远远低于正常人唾液相应成份（Ｐ〈０．０１）；复发性口疮患者治疗后，其唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶     

复发性口疮(RAU)与唾液免疫球蛋白、补体及溶菌酶活性关系的实验研究

本文对９９例复发性口疮患者，分别在初诊治疗前（发作期）和治疗后３月未发作时进行唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶含量和活性检测，并以４５名健康学生唾液相应成份作为正常对照。实验结果表明：复发性口疮患者治疗前唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶含量和活性，均远远低于正常人唾液相应成份（Ｐ＜０．０１）；复发性口疮患者治疗后，其唾液ＳＩｇＡ、ＩｇＧ、ＩｇＭ、Ｃ３、Ｃ４及溶菌酶含量和活性，均较治疗前有显著提高（Ｐ＜０．０１），其中ＩｇＧ、Ｃ４及溶菌酶含量和活性恢复较明显，与正常人唾液相应成份相似（Ｐ＞０．０５）。     

健康中、老年人唾液流率及成份的变化

本文以１５名４７－７４岁健康中、老年人和１５名１８－３３岁健康青年人为检测对象，从唾液流率及免疫球蛋白、补体（ＳｌｇＡ、ＩｇＧ、ＩｇＭＣ３、Ｃ４）和无机成分（钾、钠、钙、镁、磷）测定，观察健康中、老年人唾液成分的增令性变化。结果表明，健康中、老年人唾液流率、有机成分及无机成分并不低于健康青年人，提示中、老年人唾液腺功能无减退。     

用ELISA法分析牙周病与唾液中IgE、IgM的关系

用单克隆抗体ＥＬＩＳＡ法检测１１３名牙周组织疾病患者及正常对照受试者混合唾液ＩｇＥ及ＩｇＭ含量。结果表明：ＩｇＥ含量正常对照组明显低于患者组，特别是与青少年牙周炎组ＩｇＥ含量相差４倍。ＩｇＭ含量只有龈炎组明显增高，正常对照组与牙周炎组无明显差异。     

用ELISA法分析牙周病与唾液中IeE,IgM的关系

用单克隆抗体ＥＬＩＳＡ法检测１１３名牙周组织疾病患者及正常对照受试者混合唾液ＩｇＥ及ＩｇＭ含量。结果表明：ＩｇＥ含量正常对照组明显低于患者组，特别是与青少年牙周炎组ＩｇＥ含量相差４倍。ＩｇＭ含量只有龈炎组明显增高，正常对照组与牙周炎组无明显差异。     

维吾尔族百岁老人唾液中19种微量元素检测分析

本文意在通过对维族百岁老人唾液中微量元素检测，探讨其特点及应用价值。选临床健康的维族百岁老人32名，维族中青年人20名，用法国产等离子光量计（JCP—AES），对唾液中19种微量元素进行检测。结果唾液中钼、钙有显著性差异，唾液中锌、银、钡近似差异，大多数元素无差异。从两组检测发现维族百岁老人中，钼、钙相差很大，可能与长寿有关，而17种微量元素无异，反映唾液腺功能无下降，唾液可能用作诊断方法之一。     

维吾尔族百岁老人唾液一氧化氮的检测

本文介绍用ＮＯ检测试剂盒检测维吾尔族（下称维族）百岁老人唾液中ＮＯ含量。１材料和方法１．１样本采集１．１．１青年组（对照组）：为卫校口腔班学生３０名，维族，年龄１５～１７岁，男１３人，女１７人，身体健康，口腔正常１６人，龋１１人，牙周炎６人。均在１７...     

绝经期妇女灼口综合征唾液免疫球蛋白的研究

目的：研究绝经期妇女灼口综合征（ＢＭＳ）患者唾液流率及免疫球蛋白的变化。方法：对２９例绝经期妇女ＢＭＳ患者及２０例无ＢＭＳ的绝经期妇女的唾液流率,ｐＨ值及静态和动态混合唾液中ＳＩｇＡ,ＩｇＧ,ＩｇＭ进行观察,并行问卷调查。结果：ＢＭＳ组静态及动态混合唾液流及ｐＨ值差异无显著性意义（Ｐ〉０．０５）,而静态和动态混合唾液ＳＩｇＡ明显低于对照组,均有显著性差异（Ｐ〈０．０１）,ＩｇＭ明显高于对照组（分别     

冠龋和根龋患者唾液内致龋菌及免疫球蛋白、补体的测定

本文以３３名１８～３３岁青年人（无龋组、冠龋组）和５２名４７～７４岁中、老年人（无龋组、冠龋组、根龋组）为检测对象，对唾液内主要致龋菌（变链菌、放线菌、乳杆菌）及免疫活性物质（ＳＩｇＡ、ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ３和Ｃ４）进行了测定。结果表明，龋患者唾液内变链菌（ＭＳ、２０％蔗糖）计数显著高于无龋者，而放线菌（ＣＦＡＴ）、乳杆菌（Ｒｏｇｏｓａ）在两者之间无显著差异。龋患者唾液ＳＩｇＡ高于无龋者，且在青年人中存在显著差异。中老年龋患者ＩｇＧ和ＩｇＭ显著增高，可能与代偿平衡有关。     

免疫组织化学法在口腔黏膜白色病变病理诊断中的意义研究

目的：研究免疫球蛋白（Ig）和补体（C）在口腔扁平苔藓（OLP）、盘状红斑狼疮（DLE）和慢性增殖型念珠菌病（CHC）中的表达,以期找出对口腔黏膜白色病变有辅助检查意义的更简便、有效的方法。方法：运用免疫组织化学非生物素二步法对OLP、DLE和CHC进行检测,并将结果进行统计学分析。结果：免疫组织化学染色显示免疫复合物在三组基底膜区和结缔组织中单核细胞的阳性表达率有统计学差异（P〈0.05）。DLE组的基底膜区有免疫复合物的多元沉积。结论：运用免疫组织化学非生物素二步法检测Ig和C在口腔黏膜白色病变中的表达对其鉴别诊断有一定的辅助意义。     

Salivary immunoglobulin concentrations in predentate and edentulous mouths

The concentrations of IgA, IgG and IgM in whole saliva of predentate babies, dentate adults, and edentulous old people, and the salivary IgG concentration of dentate old people, were determined using a solid phase radioimmunoassay. A highly significant difference in IgG concentrations was found between toothless and dentate people. The average IgG concentration in the saliva of edentulous old people was 5.2 mg/1, which was about 20% of that of dentate old people. The salivary IgG content of babies was only 1.7 mg/1, which was in concordance with the low postnatal serum IgG values. The salivary IgA level in this age group was also low. The edentulous old people tended to have decreased IgM values, and the predentate babies tended to have increased IgM values compared with the dentate adults.     

Salivary immunoglobulin concentrations in predentate and edentulous mouths

Abstract — The concentrations of IgA, IgG and IgM in whole saliva of predentate babies, dentate adults, and edentulons old people, and the salivary IgG concentration of dentate old people, were determined using a solid phase radioimmunoassay. A highly significant difference in IgG concentratiorms was found between toothYess and dentate people. The average IgG concentrarion in the saliva of edentulous old people was 5.2 q/l, which was about 20° of that of dentate old people. The salivary IgG content of babies was only 1.7 q/I, which was in corxordance with the lovv postnatal serum IgG values. The salivary IgA level in this age group was also low. The edentulous old people tended to have decreased IgM values, and the predentate babies tended to have increased IgM values compared with the dentate adults.     

In situ characterization of the inflammatory cell infiltrates of hyperplastic denture stomatitis

Cryostat sections from 14 surgical specimens were examined to determine whether selected factors of the immune response related to histopathological reactions are present in the palatal mucosa affected by hyperplastic denture stomatitis. By means of various immunological techniques the presence of IgG, IgA, IgM, complement factor C3c, receptors for the Fc region of IgG (FcR) and for complement factor C3b (C3bR), T lymphocytes, and macrophages were studied. The inflammatory infiltrate was mainly located in the papillary part of the lamina propria. IgG, IgA, and IgM appeared both in plasma cells and intercellularly. FcR, C3bR, and T lymphocytes were present in the areas with inflammatory cell infiltrate. Macrophages were found in the papillary part of the lamina propria and within the epithelium. The immunological response in the mucosa affected by denture stomatitis was in many respects similar to that of marginal and apical periodontitis. We conclude that hyperplastic denture stomatitis is a complex inflammatory lesion showing elements of both humoral and cellular immune responses.     

Positional Variations of Impacted Hyperodontic Teeth: A Clinical and Radiological Study

Cryostat sections from 14 surgical specimens were examined to determine whether selected factors of the immune response related to histopathological reactions are present in the palatal mucosa affected by hyperplastic denture stomatitis. By means of various immunological techniques the presence of IgG, IgA, IgM, complement factor C3c, receptors for the Fc region of IgG (FcR) and for complement factor C3b (C3bR), T lymphocytes, and macrophages were studied. The inflammatory infiltrate was mainly located in the papillary part of the lamina propria. IgG, IgA, and IgM appeared both in plasma cells and intercellularly. FcR, C3bR, and T lymphocytes were present in the areas with inflammatory cell infiltrate. Macrophages were found in the papillary part of the lamina propria and within the epithelium. The immunological response in the mucosa affected by denture stomatitis was in many respects similar to that of marginal and apical periodontitis. We conclude that hyperplastic denture stomatitis is a complex inflammatory lesion showing elements of both humoral and cellular immune responses.     

Salivary IgA and IgG subclasses in oral mucosal diseases

OBJECTIVE: It was hypothesized that serum levels of immunoglobulins may play a role in the pathogenesis of oral mucosal diseases, or reflect clinical changes in these conditions, but little is known about the role of salivary immunoglobulins in the pathogenesis of these diseases. The aim of this study was to investigate possible alterations in salivary immunoglobulin A (IgA) and IgG subclasses in patients with oral mucosal inflammatory diseases. SUBJECTS and METHODS: Levels of IgG1, IgG2, IgG3 and IgG4 were determined by enzyme-linked immunosorbent assay (ELISA), and IgA1 and IgA2 by radial immunodiffusion in the resting whole saliva of 31 patients with acute recurrent aphthous ulceration (RAU) (and followed in remission), 11 patients with chronic hyperplastic candidal infection (CHC), 12 patients with Sjogren's syndrome (SS), six patients with oral lichen planus (OLP), and 18 healthy volunteers using the normal saliva as a comparison point for all. RESULTS: IgG and IgA subclasses were increased in OLP. In CHC all IgG subclasses were increased while IgA1 was decreased, IgG1, IgG3 and IgG4 levels were increased in SS, while all IgG subclasses as well as IgA2 were increased in acute RAU in comparison with healthy controls. No differences in any immunoglobulin subclasses between major and minor acute RAU were found. In remission, IgG1 and IgG4 returned to normal values while IgG2, IgG3, and IgA2 remained increased in patients with RAU. CONCLUSION: Salivary immunoglobulin subclasses vary in different oral mucosal conditions and may play a role in oral mucosal inflammatory diseases and/or reflect clinical changes in these conditions.     

Serum immunoglobulins and complement (C'3) in oral lichen planus

Serum immunoglobulins and complement (C'3) were determined by single radial immunodiffusion according to the method of Mancini and co-workers in fifty patients with oral lichen planus and twenty persons with clinically normal oral mucosa. Significantly increased levels of serum IgG (p less than 0.05) and a significant reduction of serum IgA concentration (p less than 0.05) in the experimental group as compared with normal controls were observed. Mean serum IgM and complement (C'3) levels were similar in patients and controls. No correlation between disease variety or extensiveness and immunoglobulin or complement levels was noticed. These results suggest that patients with oral lichen planus may have a generalized immunologic disorder in which humoral immunity is disturbed. Whether humoral immunity is of etiologic significance, contributes to the disease process, or, finally, represents an event secondary to the pathologic changes seen in the disease remains to be determined.     

Salivary antimicrobial proteins and mutans streptococci in tonsillectomized children.

Whole saliva from 53 children who had been tonsillectomized when they were younger than 4 years old was analyzed for selected antimicrobial proteins and oral mutans streptococci 3-4 years after the operation. The results were compared with those from age- and gender-matched control children with no history of tonsillectomy. The salivary analyses comprised both immune (total IgA, IgG and IgM) and selected nonimmune (lactoferrin, myeloperoxidase, salivary peroxidase) antimicrobial proteins. Specific IgA and IgG antibodies against viral antigens (adeno-, cytomegalo-, respiratory syncytial- and Epstein-Barr-viruses) and against Streptococcus mutans cells were quantitated in both groups. The tonsillectomized children had statistically significantly higher concentrations of all immunoglobulin isotypes (P 0.001) as well as of lactoferrin (P less than 0.005), and myeloperoxidase (P less than 0.001) in saliva. However, no differences were found in the numbers of cariogenic mutans streptococci or in the total oral aerobic flora. In line with the streptococcal counts, no differences existed in anti-S. mutans IgA or IgG titers between the groups. Most antibodies against viruses, especially of IgG isotype, were significantly (P less than 0.001) higher in saliva of tonsillectomized children than in that of the controls. The results suggest that, within a long run, the humoral immune status of human saliva is not weakened by tonsillectomy. Also, mainly serum-derived antimicrobial proteins (myeloperoxidase, lactoferrin, IgG) exist in high concentrations in whole saliva after tonsillectomy.     

Immunoglobulin isotypes in human minor gland saliva

Lower labial, upper labial, palatine (minor), and parotid (major) gland saliva samples from 18 young adult males were quantitatively assayed for the presence of IgA1, IgA2, IgM, and IgG. The mean (+/- standard deviation) concentrations of IgA (sum of IgA1 + IgA2) were 79 +/- 42 micrograms/mL (parotid), 111 +/- 42 micrograms/mL (lower labial), 69 +/- 72 micrograms/mL (upper labial), and 88 +/- 68 micrograms/mL (palatine). Total IgA concentrations were positively correlated among different minor-gland samples from the same subject, although these correlations did not reach significance. Upper-labial-gland saliva samples contained significantly (at least p less than 0.05) lower concentrations of IgA1 than those found in parotid or lower-labial minor-gland secretions. All three minor-gland sources of saliva contained significantly (p less than 0.002) higher levels of IgG than did parotid saliva. Upper-labial fluids had significantly (p less than 0.02) higher IgG concentrations than lower-labial saliva. IgM could be detected in 89% of parotid saliva samples and 75% of the palatine saliva samples. Palatine IgM concentrations (8.2 +/- 17.8 micrograms/mL) were significantly (p less than 0.05) higher than parotid IgM concentrations (0.6 +/- 0.4 micrograms/mL). IgM was detected much less frequently and at lower concentrations in lower- and upper-labial-gland saliva. These data reveal that minor-gland saliva from different oral sites may contain distinctive immunoglobulin isotype patterns, and expressions of host defense may vary within each micro-environment.     

The role of salivary immunoglobulins (secretory IgA, IgM, IgG) in caries prevalence and primary B-cell deficiency

The role of salivary immunoglobulins (IgA, IgM, IgG) in caries etiology is not yet clearly known. Our aim was to study whether there might be a connection between the amount of immunoglobulins in the saliva and caries prevalence. It was found that there was a significant difference (p < 0.05 or less) in secretory IgA, IgM, IgG levels, as well as DMF-T, DMF-S (approx) indices between selective IgA deficient, hypogammaglobulinemic, and healthy children, who had primary teeth.