产黑色素类杆菌群在牙髓坏死和根尖周疾病中的意义

本语言利用抗产黑色素类杆菌群共同抗原的单克隆抗体间接免疫荧光法，检测了101例牙髓坏死、根尖周病变感染根管中的产黑色素类杆菌群菌株，发现慢性根尖周炎急性发作时该菌群检出率明显上升，研究发现自发痛、叩痛、尖周区肿根管臭味、根尖阴影、根管渗液的出现与产黑色素类杆菌群有一定关系。     

黄芩对牙髓卟啉单胞菌产丁酸影响的实验研究

目的　通过研究黄芩对牙髓卟啉单胞菌生长代谢的影响,探讨黄芩治疗牙髓根尖周病的作用机制。方法　采用试管两倍稀释法测定黄芩对牙髓卟啉单胞菌(P.e)的最小抑菌浓度(MIC)。采用高效液相色谱仪测定低于 MIC4个浓度的黄芩对P.e产丁酸的影响。结果　黄芩对P.e的MIC为100 mg/L。随着药物浓度升高,P.e产丁酸量降低,其值分别为(3·527±0·009) mg/L,(3·048±0·005) mg/L,(2·490±0·011) mg/L,(2·209±0·016)mg/L。结论　黄芩对P.e生长、代谢有一定的抑制作用。     

产黑色素类杆菌群菌株免疫学特点研究

本研究测试了8株产黑色素类杆菌群菌株免疫血清的特异性,发现所制备的抗血清均不与非类杆菌属的细菌发生反应,而在产黑色素类杆菌群菌株间,抗血清存在不同程度的交叉反应,其中躯体类杆菌抗血清特异性相对较强。本文讨论了免疫方式对细菌抗体效价的影响。     

感染根管内产黑色素类杆菌的定植

目的:了解感染根管内产黑色素类杆菌(BPB)的定植情况.方法:采用16S rDNA PCR技术检测5种BPB在牙髓炎、慢性根尖周炎患牙根管内的定植情况.统计学分析5种BPB在感染根管内检出率的差异及菌种间相互关系.结果:BPB在感染根管内总检出率为60%,其中牙髓卟啉单胞菌(Pe)、变黑普氏菌(Pn)、牙龈卟啉单胞菌(Pg)、产黑普氏菌(Pm)、中间普氏菌(Pi)检出率分别为38%、32%、30%、18%、14%.Pe、Pn检出率明显高于Pm、Pi(P<0.05).Pe在慢性根尖周炎组检出率(50%)明显高于牙髓炎组检出率(20%)(P<0.05).Pn、Pg、Pi和Pm在慢性根尖周炎组检出率(43.33%、36.67%、16.67%、16.67%)与牙髓炎组检出率(20%、20%、10%、15%)无明显差异(P>0.05).Pg/Pe、Pg/Pn、Pe/Pn之间存在正相关.结论:BPB是感染根管内的优势菌群,是牙髓炎和慢性根尖周炎共有的致病菌.Pg/Pe、Pg/Pn、Pe/Pn常定植于同一根管.     

牙龈类杆菌内毒素与牙周炎关系的研究 Ⅲ.牙龈类杆菌单克隆抗…

应用杂交瘤技术建立了三株分泌牙龈类杆菌单克隆抗体的细胞系ＣＹ－４、ＣＹ－５和ＣＹ－６。ＣＹ－４、ＣＹ－５和ＣＹ－６分泌的单克隆抗体均属ＩｇＭ，能与牙龈类杆菌内毒素发生特异性结合，且经ＥＬＩＳＡ证实具有较高的种特异性。该３株杂交瘤细胞体外培养７个月余和冻存３个月后复苏培养仍能稳定地分泌单克隆抗体。     

黄芩对牙龈卟啉单胞菌生长、形态影响的体外实验

目的：探讨黄芩在牙髓、根尖周病治疗中的基础理论。方法：采用试管两倍稀释法测定黄芩对牙龈卟啉单胞菌的最低抑菌浓度（MIC）；用扫描电镜观察低于MIC的3个浓度的黄芩对牙龈卟啉单胞菌形态的影响。结果：黄芩对牙龈卟啉单胞菌MIC值为1mg/mL；黄芩改变了牙龈卟啉单胞菌菌体的正常形态。结论：黄芩对牙龈卟啉单胞菌的生长、形态有抑制作用。     

产黑色素G-厌氧杆菌在牙周炎病人龈下菌斑中的分布

目的：研究牙龈卟啉单胞菌（P.g)，中间型普氏菌（P.i)和变黑普氏菌(P.n)在牙周炎病人病变部位和正常部位龈下菌斑中的分布。方法：选择64例慢性牙周炎患者，取龈下菌斑，经厌氧培养，挑取产黑色素菌落，纯化增菌保种，经多聚酶链反应鉴定P.g,P.i和P.n。结果：共对614株产黑色素G^-厌氧杆菌进行多聚酶链反应鉴定，得到207，112和49株P.G,P.i和P.n。在病变部位和正常部位，P.g的检出率分别是35．9％和28．1％，两者无统计学差异，P.i的检出率分别是28．1％和12．5％，有统计学差异（P＜0．05），P.n的检出率分别是14．8％和9．4％，无统计学差异。结论：提示G^-厌氧杆菌是作为内源性致病菌在特定条件下过度生长导致牙周破坏。     

根管超声清理器械对产黑色素类杆菌群的体外实验效应

本研究评价了超声根管清理器材对6株产黑色素类杆菌国际标准菌标菌株的效应。结果表明:经5min的超声处理,细胞数仅减少40～55％,说明单纯超声的物理作用不会使根管内重要厌氧菌数明显减少。     

牙龈类杆菌内毒素与牙周炎关系的研究 Ⅲ.牙龈类杆菌单克隆抗体的研制

应用杂交瘤技术建立了三株分泌牙龈类杆菌单克隆抗体的细胞系CY—4、CY—5和CY—6。CY—4、CY—5和CY—6分泌的单克隆抗体均属IgM,能与牙龈类杆菌内毒素发生特异性结合,且经ELISA证实具有较高的种特异性。该3株杂交瘤细胞体外培养7个月余和冻存3个月后复苏培养仍能稳定地分泌单克隆抗体。     

产黑色素拟杆菌群与牙周病发病关系的临床研究——牙龈拟杆菌与成年人牙周炎

本文从临床筛选了46例成年人牙周炎患者牙周袋内标本和其中12例同一口腔内非牙周炎区龈沟内标本，以及进行了以局部治疗为主的19例综合治疗后同一牙周袋内标本，进行了厌氧菌检查，分离鉴定产黑色素拟杆菌群，又重点进行了牙龈拟杆菌的鉴定，分析了它们与成年人牙周炎的发病, 及与各种牙周状态的关系，并实验证明清除牙周袋内菌斑及局部病灶的疗法是最重要的牙周治疗手段。     

牙源性脓肿标本中的产黑色素类杆菌群菌株分离鉴定

本文对４４例牙源性脓肿（尖周脓肿２４例，牙周脓肿１２例，冠周脓肿８例）脓液标本中的产黑色素类杆菌群菌株进行分离、培养、鉴定。其中３８例存在产黑菌群，以中间型类杆菌阳性率最高。牙龈类杆菌在牙周脓肿中检出率最高。２４例尖周脓肿可分离出６株牙髓类杆菌而其它两种牙源性脓肿则无牙髓类杆菌，推测该菌与牙髓尖周感染的病理过程有关。     

钇/羟基磷灰石纳米微晶对口腔细菌生长繁殖的影响

目的：评价不同钇含量羟基磷灰石(HA)纳米微晶对口腔细菌生长繁殖的影响，为研制具有一定抑菌作用的种植体涂层及高强度复合树脂等口腔生物材料奠定基础。方法：采用厌氧菌连续培养技术，比较HA纳米微晶、1mol％和5mol％钇／HA纳米微晶对构成菌斑的主要细菌——变形链球菌、远缘链球菌、血链球菌、粘性放线菌及牙龈卟啉菌生长繁殖的影响，通过记录菌落计数来描述不同纳米微晶对细菌生长繁殖的作用。结果：HA纳米微晶对变形链球菌、血链球菌、粘性放线菌的生长繁殖均有抑制作用，而对远缘链球菌、牙龈卟啉单胞菌有促进生长作用；含钇1mol%，和5mol％的钇／HA纳米微晶均对变形链球菌、远缘链球菌、血链球菌、粘性放线菌有抑制作用，而对牙龈卟啉菌无抑制作用。结论：钇／HA纳米微晶能不同程度的抑制口腔细菌的生长繁殖。     

Pigmented villonodular synovitis of the temporomandibular joint: a case report and the literature review

Pigmented villonodular synovitis (PVNS) is an uncommon benign proliferative disorder of synovium that may involve joints, tendon sheaths, and bursae. It most often affects the knees, and less frequently involves other joints. It presents in the temporomandibular joints (TMJs) extremely rarely. The authors report an elderly female patient with PVNS of the TMJ with skull base extension, who had traumatic history in the same site. It was diagnosed through core-needle biopsy, which was not documented in the literature. Radical excision and follow-up for 7-8 years was recommended because of the reported malignant transformation and high recurrence rate. This case and previously reported cases in the literature are reviewed and discussed.     

Effects of glucose on formation of cytotoxic end‐products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis

Black-pigmented bacteria which produce cytotoxic metabolic end-products and cell membrane-associated proteases have been reported to play an important role in the pathogenesis of periodontal diseases. These bacterial virulence factors can be modified by the environmental conditions including nutrients supplied variously into the oral cavity. Although glucose is one of the most essential nutrients for oral bacteria, the exogenous supply of glucose may be discontinuous and the glucose concentration in a periodontal pocket may be influenced by the depth of the periodontal pocket. Therefore, effects of glucose as an environmental factor on the virulence factors of Prevotella intermedia, Prevotella nigrescens and Porphyromonas in the presence o glucose.,bo t P. intermedia and P. nigrescens markedly decreased the production of cytotoxic end-products including succinate.,isobutyrate,isovalerate and ammonia, although their growth was increased. Furthermore, the proteolytic activities such as immunoglobulin- albumin- and casein-degrading activities of these bacteria were decreased in the presence of glucose. On the other hand, no effect of glucose on the metabolic activity of P gingivalis was observed. These results suggest that pathogenicity of P. intermedia P. nigrescens may be decreased by the presence of glucose.     

Antibacterial Activity of Endodontic Sealers against Planktonic Bacteria and Bacteria in Biofilms

Introduction

The aim of this study was to investigate the antibacterial activity of 4 endodontic sealers against bacteria planktonic grown or in biofilms commonly detected from persistent and secondary endodontic infections.

柠檬提取物对口腔致龋菌生长影响的研究

目的:检测柠檬提取物的生物安全性,研究柠檬提取物对主要致龋菌生长的影响,为进一步机制研究提供依据。方法:柠檬提取物ICR小鼠急性毒性试验(LD50试验):实验分两组,实验组柠檬水提浓缩液按0.05ml/10g.bw灌胃,空白对照组室温、阳光等饲养条件与实验组相同。饲养14d观察动物反应,第15天处死,解剖检查脏器情况;采用琼脂稀释法研究柠檬提取物对5种主要致龋菌(变异链球菌、血链球菌、内氏放线菌、远缘链球菌、嗜酸乳杆菌)生长的抑制作用,并采用菌落计数法检测柠檬提取物对5种细菌生长的影响。结果:ICR小鼠在14d观察期中未发现死亡现象,处死后检查各脏器无异常现象。柠檬提取物对变异链球菌、血链球菌、远缘链球菌的最小抑菌浓度MIC(minimum inhibitory concentration)为4.5g/L,对嗜酸乳杆菌和内氏放线菌的MIC为2.25g/L。5种致龋菌在柠檬提取物作用下,其生长曲线均在第6小时左右出现明显的转折点,实验组与阴性组的细菌菌量均有显著性差异(P<0.05)。结论:柠檬提取物样品LD50大于5000mg/kg.bw,属于实际无毒物质;柠檬提取物对口腔致龋菌的生长有抑制作用。     

Detection of Sulphate-Reducing Bacteria and Others Cultivable Facultative Bacteria in Dental Tissues

Aim

To detect for the presence of sulphate-reducing bacteria (SRB) and evaluate the possible association between SRB and cultivable facultative bacterial of oral sites with different periodontal conditions.

Methods

The study was carried out on 9 samples from different oral sites in 8 patients (two samples were collected from the same patient). Material was collected using modified Postgate E culture medium, indicated for the growth and isolation of SRB. In addition, a reducing solution for anaerobic bacteria was used as a transport solution for facultative bacteria and identified by polymerase chain reaction amplification (PCR) and sequencing of the 16S rRNA gene.

Results

SRB was found in 3 patient samples: the first in a root fragment, the second in a root fragment and a healthy tooth with vertical bone loss and a mobility degree of 3; and the third in a healthy tooth extracted for orthodontic treatment. In the final patient, the cultivable facultative species Lactobacillus casei was identified. Other facultative bacterial species were identified in patient 5 (Kurthia Gibsonii) and patient 7 (Pseudomonas aeruginosa).

Conclusions

The detection of SRB in different dental tissues with distinct periodontal features demonstrated that new studies need to be developed in order to determine the true role of SRB in the oral microbiota. In addition, it was possible to verify the presence of Lactobacillus casei together with SRB in one sample.     

全菌蛋白SDS-PAGE技术鉴别口腔细菌分型的研究

采用十二烷基磺酸钠－聚丙烯酰胺凝胶是民泳结合考马斯亮蓝染色和银染色法，鉴别口腔细菌。结果表明，同一菌株用不同菌体破碎方法，不同批次处理的样品，不同次电泳、蛋白区带的不同染色法所得蛋白图型基本一致；而不同种的细菌电泳和扫描图型均不相同。     

根管残余细菌对根管治疗预后的影响

目的　探讨根管预备后残留细菌对根管治疗预后的影响。方法　选取50颗患慢性根尖周炎的单根管牙,分为实验组和对照组。实验组21颗患牙采用逐步后退法及超声冲洗预备根管,根管预备后取样做细菌培养, 立即充填根管,一次性完成根管治疗。对照组采用常规根管治疗方法治疗。根管治疗后6个月、1年及2年复查。结果　①两组患牙根管治疗预后无明显差异。②实验组患牙根管预备后残余细菌与根管治疗预后无明显关系。 ③患牙术后疼痛与根充情况和术前症状无明显关系。结论　近期观察见经过彻底的根管预备,根管内微量残余细菌对根管治疗预后无明显影响。