复方青黛片治疗难治性急性早幼粒细胞白血病32例不良反应护理

目的:探讨复方青黛片治疗难治性急性早幼粒细胞白血病(APL)不良反应的护理方法.方法:用纯中药制剂复方青黛片治疗难治性APL 32例,对其不良反应进行临床观察和护理.结果:本组患者中完全缓解30例,未缓解2例;其中不良反应8例,占25.00%.结论:加强对患者的临床观察,耐心细致地做好心理护理,是提高复方青黛片临床疗效和安全性的关键.     

复方甘草酸苷联合青黛丸治疗寻常型银屑病的疗效观察

目的:观察复方甘草酸苷联合青黛丸治疗寻常型银屑病的疗效.方法:对照组给予口服复方青黛丸6 g,3次/d,外用3%水杨酸乳膏;治疗组在以上治疗基础上加服复方甘草酸苷片.结果:治疗组疗效优于对照组,差异有统计学意义.结论:复方甘草酸苷联合复方青黛丸治疗寻常型银屑病取得良好疗效,且无明显不良反应.     

复方青黛片治疗慢性粒细胞白血病致慢性砷中毒一例

患者,男,58岁。1980年4月因低热、乏力、反复咽喉部疼痛入院。体检:胸骨压痛、脾脏肿大平脐,外周血ＷＢＣ1108×109/Ｌ,骨髓穿刺示慢性粒细胞白血病(ＣＧＬ),Ｐｈ染色体( )。给马利兰、羟基脲、靛玉红等治疗,病情稳定。1996年1月复查骨髓:原始粒细胞016,早幼粒细胞020,诊断ＣＧＬ加速期。即给复方青黛片(由大连解放军中医血液病专科中心提供,025ｇ/片)口服,每日3次,每次6～8片,同时口服肝泰乐,定期复查血象、骨髓象及肝肾功能。9个月后骨髓象示完全缓解(ＣＲ)。除手掌、足底过度角化及色素沉着外无其它不良反应。遂停复方青黛片改用羟基脲和…     

雄黄对NB4及HL-60细胞的促凋亡作用

目的：研究复方青黛片的主要成分雄黄治疗急性早幼粒细胞白血病（ＡＰＬ）的机制。方法：应用细胞形态、ＤＮＡ电泳及流式细胞仪测定等多项方法，在体外研究雄黄诱导ＮＢ４细胞和ＨＬ６０细胞凋亡的作用。结果：雄黄具有诱导ＮＢ４细胞和ＨＬ６０细胞凋亡作用，雄黄浓度在２５．０～２００．０ｍｇ／Ｌ之间这种诱导作用无明显差异。浓度为１２．５ｍｇ／Ｌ，培养７２小时，ＮＢ４细胞表现典型的凋亡特征而ＨＬ６０细胞则无。二硫化二砷（Ａｓ２Ｓ２）与雄黄在相同条件下实验结果一致。结论：雄黄诱导ＮＢ４和ＨＬ６０细胞凋亡是其主要成分Ａｓ２Ｓ２的作用，而非其所含少量其它重金属元素的作用。在一定范围，其作用随时间和浓度增加而增强。雄黄是复方青黛片治疗ＡＰＬ取得高缓解率的中药成分。     

三氧化二砷治疗急性早幼粒细胞白血病复发的临床观察

急性早幼粒细胞白血病(APL)的诱导治疗主要是全反式维甲酸(ATRA),完全缓解率可达到85～95%。但其引起的白细胞过高、维甲酸综合征及快速发展的耐药性,严重影响患者长期存活,且对其复发患者再缓解率低。近年来,一些学者用复方青黛片、癌灵一号等     

复方青黛丸致便血2例

复方青黛丸致便血２例李国良，梁洪彦０６１００１河北省沧州市中心医院复方青黛丸具有清热解毒、消斑化瘀等功效，临床治疗银屑病及其它皮肤病，主要不良反应为腹泻。笔者用复方青黛丸治疗银屑病后发生便血２例，现报告如下。［例１］患者男性，４６岁。于１９９４年１月...     

复方青黛饮对角质形成细胞增殖影响的实验研究

目的:研究复方青黛饮治疗银屑病的作用机制.方法:腹腔注射乙烯雌酚有引起小鼠上皮细胞增殖的作用,小鼠尾部表皮天然缺少颗粒层,这两种特征与银屑病病理相似.不同组别的小鼠以复方青黛饮或郁金银屑片灌胃,观察其对角质形成细胞增殖的调节作用.结果:与对照组比较,复方青黛饮有较好的降低嗜银蛋白含量和小鼠阴道上皮细胞有丝分裂,以及促使角质形成细胞分化的作用.结论:复方青黛饮治疗银屑病的作用机制与调节角质形成细胞增殖有关.     

参蝥复方对L615白血病小鼠红细胞免疫功能的影响

本文报道了参蝥复方（主药为红参、青黛）对正常６１５小鼠及Ｌ６１５白血病小鼠红细胞免疫功能的影响。实验结果表明，参蝥复方能明显提高正常实验小鼠红细胞Ｃ３ｂ受体花环率，而对红细胞免疫复合总环率及自然肿瘤红细胞花环率和直向肿瘤红细胞花环率影响不在；对Ｌ６１５白血病小鼠，参蝥复方则可全面改善上述各项红细胞免疫指标。提示参蝥复 改善机体红细胞免疫功能的作用。     

复方青黛丸（浓缩丸）联合NB-UVB照射及中药熏蒸治疗玫瑰糠疹疗效观察

目的:探讨复方青黛丸(浓缩丸)联合NB-UVB照射及中药熏蒸治疗玫瑰糠疹的疗效。方法:采用随机对照试验将78例患者随机分为两组:治疗组40例采取复方青黛丸(浓缩丸)联合NB-UVB照射及中药熏蒸治疗。对照组38例采取复方氨肽素片联合醋酸曲安奈德乳膏外用治疗,两组患者均于疗程结束后观察疗效。结果:78例均完成试验,治疗组有效率为95.0%,对照组为65.8%,治疗组与对照组有效率比较,差异有统计学意义(χ2=10.70,P>0.01)。治疗组治愈时间为(6.23±2.08)d,明显低于对照组治愈时间(15.57±3.45)d,差异有统计学意义(P<0.01)。结论:复方青黛丸(浓缩丸)联合NB-UVB照射及中药熏蒸治疗玫瑰糠疹有效率高,疗程短,不良反应小,获得良好的临床疗效。     

复方青黛丸与阿维A及卡泊三醇治疗中、重度寻常性银屑病的临床效果研究

分析复方青黛丸与阿维A及卡泊三醇治疗中、重度寻常性银屑病的临床效果。以2015年3月~2016年10月到本院门诊参与治疗的41例中、重度寻常性银屑病患者为对象,电脑随机分组2组,即对照组(20例)与观察组(21例)。对照组,联合复方甘草酸苷与阿维A治疗,观察组,联合复方青黛丸、阿维A及卡泊三醇治疗,对比分析两组患者治疗效果。对于治疗总有效率,观察组为90.4%,对照组为75%,组间差异显著(P<0.05)。复方青黛丸与阿维A及卡泊三醇治疗中、重度寻常性银屑病,效果显著,值得广泛推广。     

Surface modified composite nanofibers for the removal of indigo carmine dye from polluted water

Surface coated magnetite nanoparticles (Fe₃O₄ NPs) with 3-mercaptopropionic acid were immobilized on amidoximated polyacrilonitrile (APAN) nanofibers using electrospinning followed by crosslinking. The prepared composite nanofibers were characterized with Scanning Electron Microscopy (SEM), and Fourier Transform Infrared analysis (FTIR). The composite nanofiber was evaluated for the removal of indigo carmine dye from aqueous solutions. The effects of contact time, initial dye concentration, solution pH and adsorption equilibrium isotherms were studied. The adsorption of indigo carmine was found to be greatly affected by solution pH. The maximum loading capacity was determined to be 154.5 mg g⁻¹ at pH = 5. The experimental kinetic data were fitted well using a pseudo-first order model. The adsorption isotherm studies showed that the adsorption of indigo carmine fits well with the Langmuir model. The reuse of the composite nanofiber was also investigated in which more than 90% of indigo carmine was recovered in 5 min. The results of stability studies showed that the adsorption efficiency can remain almost constant (90%) after five consecutive adsorption/desorption cycles.

Surface coated magnetite nanoparticles (Fe₃O₄ NPs) with 3-mercaptopropionic acid were immobilized on amidoximated polyacrilonitrile (APAN) nanofibers using electrospinning followed by crosslinking.     

Effects of indigo carmine intravenous injection on oxygen reserve index (ORi™) measurement

To retrospectively investigate the effects of indigo carmine intravenous injection on oxygen reserve index (ORi?) in 20 patients who underwent elective gynecologic surgery under general anesthesia. The study subjects were patients who underwent elective gynecologic surgery under general anesthesia between April 2016 and January 2017, and were administered a 5-ml intravenous injection of 0.4% indigo carmine for clinical purposes during surgery with ORi monitoring. Changes in ORi within 20 min after indigo carmine injection were observed. A relevant decrease in ORi was defined as ≥?10% reduction in ORi from pre-injection level. ORi rapidly decreased after indigo carmine intravenous injection in all patients. In 10 of 19 patients, ORi decreased to 0 after indigo carmine injection. The median lowest value of ORi was 0 (range 0–0.16) and the median time to reach the lowest value of ORi was 2 min (range 1–4 min) after injection. ORi values returned to pre-injection levels within 20 min in 13 of 19 patients, and the median time to return to pre-injection levels was 10 min (range 6–16 min) after injection. During ORi monitoring it is necessary to consider the rapid reduction in ORi after intravenous injection of indigo carmine.     

Preparation of multi-layer nylon-6 nanofibrous membranes by electrospinning and hot pressing methods for dye filtration

We report the formation of multi-layer nylon-6 (PA-6) nanofibrous membranes by electrostatic spinning coupled with a hot pressing process. The structure and porosity of multi-layer PA-6 nanofibrous membranes were characterized using a scanning electron microscope and the N₂ nitrogen adsorption and desorption isotherms. We show that multi-layer PA-6 nanofibrous membranes can be used for efficient and continuous indigo dye filtration. Under the condition of constant pressure at the 0.1 MPa dead end filtration, continuous filter for a period of time, the filtration efficiency for indigo dye increases with the increase of the number of layers in PA-6 nanofibrous membranes. The 10 layer PA-6 nanofiber membrane can completely remove the indigo dye, and early filtration flux was high, with extended time, the filtration flux decline and gradually stabilized.

The formation of multi-layer nylon-6 (PA-6) nanofibrous membranes by electrostatic spinning coupled with a hot pressing process, and they can be used for efficient and continuous indigo dye filtration.     

水交换法肠镜检查中加入靛胭脂提高腺瘤检出率的对比研究

目的:探讨水交换法中加入靛胭脂提高腺瘤检出率的有效性。方法:前瞻性入组病例,随机采用常规水交换法(对照组)或加用0.008%靛胭脂水交换法(试验组)进行结肠镜检查。研究评价的主要指标为腺瘤检出率。结果:共202例患者纳入研究,试验组和对照组各101例。两组性别分布、平均年龄、吸烟史、结肠癌家族史和肠镜检查适应证差异无统计学意义。两组盲肠插管成功率均为100%。对照组腺瘤检出率为31.7%,试验组腺瘤检出率为45.4%,两组差异有统计学意义(P0.05)。结论:加用0.008%靛胭脂的水交换法肠镜检查可显著提高腺瘤检出率。     

早期胃癌内镜特点及高危因素分析

目的探讨早期胃癌内镜下表现的高危因素,提高早期胃癌的内镜检出率。方法回顾性分析该院2016年12月-2018年6月行胃镜精查患者的临床病理资料,共245例患者247处病变。记录患者内镜诊断、病变大小、形态、颜色、是否附着黏液、病变部位、靛胭脂染色情况、窄带成像技术联合放大内镜(NBI-ME)下表现等,先用2检验分析早期胃癌的相关危险因素,再应用多因素Logistic回归模型分析早期胃癌内镜下表现的高危因素,并进行分层分析,同时探讨NBI-ME对早期胃癌的诊断敏感性和特异性。结果 2检验分析结果表明:萎缩性胃炎、病变位于胃窦部、病变形态为凹陷型、病变 1 cm、白光下存在边界、颜色发红、靛胭脂染色后不着色及存在边界、NBI-ME下观察可见边界、表面微结构不规则、微血管不规则均与早期胃癌有关;Logistic回归分析表明:靛胭脂染色后不着色(O^R=4.474,95%CI:1.576～12.701,P=0.005)、NBI-ME观察存在边界(O^R=7.608,95%CI:1.427～40.570,P=0.017)、NBI-ME观察微血管不规则(O^R=8.051,95%CI:1.509～42.949,P=0.015)为早期胃癌的独立危险因素。分层分析显示:NBI-ME观察微血管不规则联合靛胭脂染色不着色诊断早期胃癌的准确性为78.3%。NBI-ME诊断早期胃癌的敏感性为72.1%,特异性为78.9%。结论内镜下发现病变靛胭脂染色后不着色、NBI-ME观察到微血管不规则或NBI-ME观察到存在边界,提示为早期胃癌可能性较大。其中NBI-ME观察到微血管不规则联合靛胭脂染色不着色诊断早期胃癌的准确度较高。     

Adsorption and anion exchange insight of indigo carmine onto CuAl-LDH/SWCNTs nanocomposite: kinetic,thermodynamic and isotherm analysis

Two-dimensional layered materials are gaining much attention in the field of wastewater purification. Herein, we report the synthesis and characterization of an anion selective copper–aluminum-layered double hydroxide/single-walled carbon nanotubes (CuAl-LDH/SWCNTs) composite for the scavenging of organic dye indigo carmine (IC) from aqueous solution. A facile urea hydrolysis method was used for the controlled growth of the metal hydroxides over the SWCNTs. Structural characterization of the prepared materials was investigated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques. The obtained results revealed that the CuAl-LDH/SWCNTs composite has a higher potential for the removal of IC in comparison to CuAl-LDH and SWCNTs. The enhanced adsorption capacity of the composite revealed that deposition of CuAl-LDH over SWCNTs increases the active adsorption sites and promotes the interactions between the composite and IC dye via anion exchange, electrostatic, π–π, hydrogen bonding etc. Moreover, adsorption kinetics, isotherms, and thermodynamic studies have been also proposed to illustrate the mechanism of the IC adsorption onto the CuAl-LDH/SWCNTs composite. Thermodynamic parameters showed that the adsorption of IC dye onto the CuAl-LDH/SWCNTs composite was exothermic and spontaneous in nature. Intra-particle diffusion was determined to be the rate-limiting step and adsorption of IC followed the Langmuir isotherm model with the maximum monolayer adsorption capacity 294.117 mg g⁻¹ at 20 °C. The results suggest that the CuAl-LDH/SWCNTs composite is a potential material for IC adsorption in aqueous solution.

Two-dimensional layered materials are gaining much attention in the field of wastewater purification.     

Green and efficient biosynthesis of indigo from indole by engineered myoglobins

With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M⁻¹ s⁻¹) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb were found to produce the indigo product with a chemoselectivity as high as ∼80%. Based on the reaction system, we also established a convenient and green dyeing method by dyeing a cotton textile during the biosynthesis of indigo, followed by further spraying the concentrated indigo, without the need of strong acids/bases or any reducing agents. The successful application of dyeing a white cotton textile with a blue color further indicates that the designed enzyme and the dyeing method have practical applications in the future.

Myoglobin (Mb) was redesigned to a green and efficient biocatalysts for the biosynthesis of indigo from indole, exhibiting improved yield, catalytic efficiency and chemoselectivity (as high as ∼80%).     

Effects of indigo carmine intravenous injection on noninvasive and continuous total hemoglobin measurement

The effects of an intravenous injection of indigo carmine on noninvasive and continuous total hemoglobin (SpHb) measurement were retrospectively evaluated. The subjects were 21 patients who underwent elective gynecologic surgery under general anesthesia. During surgery, 5 mL of 0.4 % indigo carmine was intravenously injected, and subsequent changes in SpHb concentrations were evaluated. The results demonstrate that the pre-injection SpHb level was 10 g/dL, and the minimum post-injection SpHb level was 8.3 g/dL. The amount of decrease was 1.8 g/dL. The time to reach the minimum value was 4 min, and the time to return to the pre-injection value was 15 min. The decrease in SpHb was greater in the group with a perfusion index (PI) < 1.4 than in the group with a PI > 1.4. The assessment of SpHb after an intravenous injection of indigo carmine necessitates caution.     

慢性淋巴细胞系统白血病免疫表型分析

目的：研究国内慢性淋巴细胞系统折血病的免疫表型特点。方法：采用单参数和多参数流式细胞术分析了163例慢性淋巴细胞系统白血病的免疫表型。结果：71.8％（117/163）患者共表达CD5和B细胞标志。采用WHO引用的计分系统，将病例分为B-慢性淋巴细胞白血病（B-CLL），毛细胞白血病（HCL）和其他B淋巴细胞增殖性疾病。典型的B-CLL表达CD5、CD23、CD20、CD19、HLA-DR，但仍有部分患者表达CD22、CD11c、CD25和FMC7。CD103似为HCL最特异的标志。但仅仅依靠免疫表型难以鉴别非典型B-CLL、B细胞-幼淋巴细胞白血病（B-PLL）和外套细胞淋巴瘤（MCL），细胞遗传学或分子生物学检查将有助于鉴别诊断。以同一标本中残存的正常淋巴细胞为内参照，计算前向角光散射（FSC）指数和抗原表达指数，可定量地表示细胞的大小和抗原表达的强度，使不同的标本具有可比性。结论：免疫表型分析是诊断慢性淋巴细胞系统白血病非常有用的依据。