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1.
烧伤后血浆纤维连接蛋白的动态观察   总被引:3,自引:0,他引:3  
目的研究烧伤患者创面修复过程中24h到3周血浆纤维连接蛋白(Fn)变化规律及创面烧伤程度与Fn的关系。方法采用酶标仪比浊法检测50例正常人和35例烧伤患者。结果16例烧伤总面积>50%以上的患者伤后24h出现血浆Fn明显下降,在伤后1周仍出现第2次下降,至2周后渐渐恢复正常水平。19例烧伤总面积<50%以下的患者血浆Fn一般都处在正常范围内。7例死亡患者血浆Fn含量始终偏低。结论提示Fn的含量与烧伤后病情变化关系密切,并起着十分重要的作用  相似文献   

2.
目的探讨细胞外基质纤连蛋白(Fn)、基膜连接蛋白(Lm)与白血病细胞生长、分化之间的关系。方法应用免疫荧光法观察18例急性髓细胞白血病(AML)骨髓细胞长期培养(LTBMC)细胞外基质Fn、Lm含量的变化。结果①9例获独立生长的AML(AMLsm)骨髓细胞在培养的1~3周,Fn、Lm含量均显著低于9例未获独立生长的AML(AMLnsm);②在培养的第3周,8例AMLnsm幼稚细胞比例可降至0.05以下,而7例AMLsm未能降至0.05;③各周幼稚细胞比例分别与Fn、Lm含量相关分析示:第0~8周正常对照均呈正相关,0~4周AMLnsm均呈负相关而AMLsm仅Fn组呈负相关。结论Fn和(或)Lm的改变在AML发生、发展的某一环节起重要作用。此外,Fn、Lm含量在AMLsm与AMLnsm之间不同,这对LTBMC应用于AML自身骨髓体外净化病例的选择可能有参考价值。  相似文献   

3.
采用密度梯度分离法和Cell-ELISA法制备并测定了34例创伤住院人创伤后第1、3、7天循环MNC和PMN膜Fn水平,以及吞噬功能和血浆Fn水平。结果表明:创伤后MNC和PMN膜Fn水平呈动态变化,MNC膜Fn水平并与创伤严重程度有关。  相似文献   

4.
对17例已发生多器官衰竭(MOF)的患者,于发病后第1~20天分别进行血浆肿瘤坏死因子(TNF)与纤维连结蛋白(fn)的动态观测。结果:①全部患者病程各时间点的血浆Fn均值均低于正常值(252.12±24.72mg/L),TNP均值则明显高于正常值(5.50±0.27ug/L)。②MOF发生5天内Fn均值波动下降,第6天有明显回升达正常界限,第7天降低后再度明显回升,但于第10天又见下降而后波动在180.00~230.00mg/L。③TNF均值在MOF发生5天内逐日上升,达到45.001ug/L,而后虽有一定波动,但基本持续在32.00ug/L以上。作者认为:与正常人比较,TNF的增高变化比Fn降低变化更为明显,且较稳定,故作为诊断参考指标1NF比Fn更有价值。  相似文献   

5.
胫骨平台骨折后需早期康复锻炼但不宜过早负重锻炼   总被引:2,自引:0,他引:2  
胫骨外侧平台骨折伴内侧副韧带损伤的治疗不同于单纯的胫骨平台骨折,它不仅需要骨折的解剖复位,可靠固定,还必须修复损伤的韧带,还必需早期康复锻炼,这样才能获得良好功能。自1996年1月~2000年12月治疗5例伴内侧副韧带损伤的胫骨外侧平台骨折,获得良好效果,报告如下。1对象与方法1.1对象本组5例中,男3例,女2例;年龄27~78岁;按雍宜民外翻损伤分型犤1犦:Ⅱ度4例,Ⅲ度1例;5例均伴有内侧副韧带损伤。1.2方法均在伤后1周内采用胫骨外侧平台撬拔植骨内固定韧带修复手术。术后第1天指导病人练习股…  相似文献   

6.
血浆纤维结合蛋白在危重病患者中的变化及其意义   总被引:2,自引:0,他引:2  
纤维结合蛋白(Fibronectin,Fn)主要由肝脏及血管内皮细胞生成,具有调理素功能,可激活补体,增强单核巨噬细胞清除毒性物质、颗粒和免疫复合物功能〔1〕。Fn通过凝血因子ⅩⅡ参与凝血过程。在血管内皮损伤、胶原暴露时,Fn可以调整纤维蛋白与胶原之...  相似文献   

7.
本科自1990年以来对5例伤后超过4~6周的患儿,采用延长伸肌腱使骨折达到解剖复位,效果满意,报告如下。1资料与方法本组5例,男4例,女1例;年龄7~12岁,平均10岁。损伤原因,跌伤所致。骨折类型,属SalterHarrisⅣ型,根据骨折后骨折块移位程度4度分类法:2°2例,3°2例,4°1例。损伤至手术时间4~6周,其中2例因伤后皮肤擦伤和水泡延误手术时机。2例伤后为2°,作手法复位石膏固定,4周后复查X线片骨折块向外侧移位加重。1例4°在当地作肘关节复位小夹板固定,6周后关节功能受限而就诊。方法:(1)臂…  相似文献   

8.
探讨危重症患儿血浆纤维连接蛋白(Fn)的变化特点及其意义。测定75名危重患儿Fn含量。结果显示:其值(160.42±78.18mg/L)明显下降,与正常儿童的Fn含量(259.30±34.68mg/L)相比有显著性差异(t=5.111,P<0.01);呼吸衰竭、全身感染和中枢神经系统疾病时Fn均有降低;另外,22例门诊或中度呼吸道感染的患儿Fn与正常儿童相比无显著性差异(t=1.871,P>0.05),而与危重症患儿相比差异有显著性(t=5.409,P<0.01);严重呼吸衰竭需机械通气患儿Fn含量比呼吸衰竭不需机械通气患儿Fn降低更明显;而感染性休克患儿Fn比未合并休克患儿的Fn减低更明显。所以,小儿危重症时Fn降低主要与疾病的严重程度有关,Fn若持续降低,则提示预后不良,Fn可作为判定疾病严重程度和病情转归的指标之一,也可作为临床Fn替代治疗的一个指征  相似文献   

9.
石如海  祝善俊 《新医学》1996,27(7):351-352
本文检测了48例CHD患者血浆Fn和CEC以判断CHD患者Fn和CEC之间的相互关系。结果显示:CHD患者血浆Fn结果明显低于正常人,与血浆CEC呈显著负相关,且与病情严重程度相一致。提示:CHD患者血浆Fn和VCE损伤有一定关系,其对CHD患者的病情评估及预后判断有一定意义。  相似文献   

10.
纤维结合蛋白(fibronectin,Fn)是细胞和细胞与基质粘连的重要因子,对保持细胞正常结构和功能,促进上皮细胞移行、修复和机体免疫调控等方面起着重要作用[1~3]。自Nishida[4]将Fn引入眼科领域,已有大量的实验研究。本文应用富含Fn的...  相似文献   

11.
A study was made of the influence of the level and biological activity of plasma fibronectin of phagocytosis and opsonic activity of blood plasma in 147 patients with food toxinfections depending on a period and degree of severity of a course of disease. Plasma fibronectin concentration was determined by rocket immunoelectrophoresis. Fibronectin biological activity was assessed by cold heparin precipitation. Opsonic activity of blood plasma before and after fibronectin inactivation in it was investigated using indices of phagocytosis which was studied by optical microscopy. Food toxinfections were attended by a decrease in the level and biological activity of plasma fibronectin returning to normal during therapy and convalescence. A degree of the reduction of the level of plasma fibronectin reflected the severity of a course of food toxinfections. Phagocytic insufficiency developed in food toxinfections. One of its mechanisms was a decrease in opsonic activity of plasma fibronectin as a result of the reduction of its level.  相似文献   

12.
Platelet-associated fibronectin antigen has been identified by radioimmunoassay and immunofluorescent techniques. In radioimmunoassay, platelet fibronectin was immunochemically indistinguishable from plasma fibronectin. Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. The level of platelet fibronectin in detergent extracts of washed platelets from 12 healthy adults was 2.85 +/- 1.24 microgram/10(9) platelets. Immunofluorescence with F(ab')2 fragments of immunochemically purified antifibronectin showed that all platelets stained with a discrete punctate pattern. The identification of platelet fibronectin antigen raises the possibility that this protein may participate in platelet-platelet or platelet-surface interactions.  相似文献   

13.
To investigate the influence of neurogenic and hormonal stimuli during and after surgery on plasma fibronectin levels, 16 females undergoing cholecystectomy were studied. Eight patients received general anaesthesia, and eight also received a thoracic epidural block with local anaesthetic, which was maintained for 24 h postoperatively. The epidural group had significantly lower plasma levels of adrenaline and cortisol than the general anaesthesia group in the postoperative period. The previously well-documented early decrease in plasma fibronectin concentration following surgery was observed, and was essentially parallel with that of albumin, pre-albumin and thyroid hormones, with no differences between the groups. However, the restoration of the fibronectin level was slower in the epidural group, with significantly lower values as compared with controls at 48 and 72 h after surgery (p less than 0.01-0.001). It was concluded that the post-traumatic plasma fibronectin decrease is not mediated by neurogenic or adrenal stimuli. Such stimuli may, however, influence the subsequent restoration of the plasma fibronectin concentration.  相似文献   

14.
Reduced levels of plasma fibronectin have been implicated in the septicaemia and/or endotoxaemia that can accompany trauma or burns. Patients undergoing surgery for the relief of obstructive jaundice are also at risk of developing endotoxaemia or septicaemia. In this study, levels of plasma fibronectin were measured by immunoassay and bioassay in 16 such patients. No patient had a significantly reduced level of plasma fibronectin. Even in the case of patients developing a fatal septicaemia and general system failure there was no significant decrease in circulating fibronectin. It is concluded that measurement of plasma fibronectin is of no prognostic value and similarly infusion of fibronectin-rich cryoprecipitate would be of no therapeutic benefit.  相似文献   

15.
To investigate the influence of neurogenic and hormonal stimuli during and after surgery on plasma fibronectin levels, 16 females undergoing cholecystectomy were studied. Eight patients received general anaesthesia, and eight also received a thoracic epidural block with local anaesthetic, which was maintained for 24 h postoperatively. The epidural group had significantly lower plasma levels of adrenaline and Cortisol than the general anaesthesia group in the postoperative period. The previously well-documented early decrease in plasma fibronectin concentration following surgery was observed, and was essentially parallel with that of albumin, pre-albumin and thyroid hormones, with no differences between the groups. However, the restoration of the fibronectin level was slower in the epidural group, with significantly lower values as compared with controls at 48 and 72 h after surgery (p<0.01–0.001). It was concluded that the post-traumatic plasma fibronectin decrease is not mediated by neurogenic or adrenal stimuli. Such stimuli may, however, influence the subsequent restoration of the plasma fibronectin concentration.  相似文献   

16.
EIA was used to measure the concentration of fibronectin in plasma and cryoprecipitates of 37 patients suffering from systemic lupus erythematosus coupled with cryoglobulinemia. A definite relationship was discovered between the level of cryoglobulins and the activity of SLE, the concentration of fibronectin in plasma and liver damage. A tendency was revealed towards increase of the fibronectin content in plasma of patients with a high level of antibodies to native DNA and CIC as was a significant correlation between the concentration of cryoglobulin protein and the concentration of fibronectin in cryoprecipitates.  相似文献   

17.
A group of 10 patients with 30-70% burns were given intravenous infusions during the first 48 h following hospital admission either with fresh frozen plasma (FFP) or human plasma protein fraction ( HPPF ). FFP contained 300-400 mg/dl plasma fibronectin whereas none was detectable in HPPF . Circulating plasma fibronectin levels fell quickly in those patients receiving HPPF and levels remained low for 2-3 weeks. In those receiving FFP, plasma fibronectin remained normal during the 48-h transfusion period but fell subsequently. Fibronectin may be an important determinant in the resistance to shock and infections. Consideration should therefore be given to the use of blood products which contain fibronectin and to the monitoring of plasma levels both during the acute and recovery periods after burn injury.  相似文献   

18.
During pregnancy, significant changes occur in the hemostatic system and in the plasma levels for several plasma proteins, especially towards term. In this study changes occurring during normal pregnancy and immediately postpartum were investigated to establish adequate reference intervals for important hemostatic parameters. Blood samples were collected during pregnancy weeks 33, 36, 39 and 1-3 h after delivery from 153 healthy pregnant women with at least one previous normal pregnancy. The plasma samples were analyzed for antithrombin, von Willebrand factor (vWf), free protein S and fibronectin. Fibronectin and vWf are contact-promoting proteins responsible for adhesion and aggregation during primary hemostasis, but are also released from thrombocytes during activation of the coagulation process. Antithrombin is the most important primary physiological inhibitor of activated serine proteases related to the coagulation cascade. Protein S is a co-factor to protein C and in cooperation is also an important inhibitor of the coagulation cascade. During third-trimester pregnancy, vWf was higher than in non-pregnant women, and continued to increase postpartum. The fibronectin plasma level was mostly unchanged in comparison with non-pregnant values. Within this reference interval it gradually increased during the third trimester, but fell slightly postpartum. Antithrombin decreased slightly during the third trimester and even further, postpartum. Free protein S decreased markedly but to a stable level from week 33 to 39, decreasing even more postpartum. The present results are concordant with clinical knowledge of increased risk of thrombosis during pregnancy and early puerperium, with increased levels of vWf and fibronectin and decreased levels of antithrombin and free protein S. Clearly, current reference values based on healthy non-pregnant subjects are not usable during late pregnancy and immediately postpartum.  相似文献   

19.
Platelet adhesion to monomeric collagen types I and III, which were purified from human umbilical arteries, was studied in a perfusion chamber under well defined flow conditions. For this purpose, glass coverslips were coated with 20-30 micrograms/cm2 of collagen types I and III by spraying a solution of these collagens with a retouching air brush. Platelet deposition increased with the time of perfusion. Adhesion to both collagen types was similar in the first 3 min, but increased platelet deposition occurred on collagen type III after 3 min due to thrombus formation. Adhesion at a shear rate of 800 s-1 was strongly impaired with plasma of a patient with von Willebrand's disease or with fibronectin-free plasma. Addition of purified fibronectin to fibronectin-free plasma restored adhesion to the level obtained with normal plasma. Platelet deposition in normal plasma increased with increasing shear rates. Platelet deposition in VWD-plasma was normal at 490 s-1, but there was no increase at higher shear rates. Platelet deposition in fibronectin-free plasma was diminished at all shear rates studied from 490 to 1,300 s-1. Perfusion with a human albumin solution (HAS) to which purified Factor VIII-von Willebrand factor complex (FVIII-VWF) and fibronectin had been added gave similar platelet deposition as with normal plasma. Preincubation of collagen with FVIII-VWF and perfusion with HAS containing fibronectin, or, conversely, preincubation with fibronectin and perfusion with HAS containing FVIII-VWF, also resulted in adhesion similar to that observed in normal plasma. Similar adhesion was also observed after preincubation with both FVIII-VWF and fibronectin and subsequent perfusion with HAS alone. Sequential preincubations with first FVIII-VWF and then fibronectin, or with first fibronectin and then FVIII-VWF followed by perfusion with HAS, also gave a similar adhesion as observed with normal plasma. These data indicate that platelet adhesion to monomeric collagen types I and III is dependent on both FVIII-VWF and fibronectin. FVIII-VWF is only required at relatively high shear rates; fibronectin also at relatively low shear rates. Their complementary role in platelet adhesion suggests separate binding sites for FVIII-VWF and fibronectin on collagen. Platelet deposition on performed fibrils of collagen types I and III was also studied. Initial adhesion expressed as percentage surface coverage was similar to that found with monomeric collagen, but thrombus formation was much enhanced. Adhesion on fibrillar collagen at 800 s(-1) was impaired in VWD-plasma and fibronectin-free plasma, and was restored by addition of purified fibronectin to fibronectin-free plasma. When perfusions were performed with HAS, only addition of FVIII-VWF was required for optimal adhesion to fibrillar collagen; addition of fibronectin had no effect. These data are in contrast to the studies with monomeric collagens described above, in which the addition of both FVIII-VWF and fibronectin was required. These data are also in contrast to the observation that in plasma both FVIII-VWF and fibronectin are required for optimal adhesion to fibrillar collagen.  相似文献   

20.
The fibronectin level in the blood of patients with myocardial infarction was measured at varying times from the onset of an angina pectoris attack in order to elucidate the diagnostic importance of blood fibronectin. At the same time these patients were examined over time for the blood content of myoglobin, MB creatine kinase protein and C-reactive protein playing a well-known role in the diagnosis. The blood concentrations of these substances reached the maximal values at different times of myocardial infarction. The mean concentrations of fibronectin in the blood of patients with myocardial infarction ranged within normal starting from the first till the 28th day since the onset of an angina pectoris attack. Moreover, the mean blood fibronectin level in myocardial infarction patients did not differ within the first-third days since the disease onset from that in patients with a clinical picture of unstable angina pectoris which was not accompanied by the development of myocardial infarction. Based on the data obtained it is concluded that measurement of blood fibronectin level does not play any diagnostic role in myocardial infarction. On the other hand, progressive increase in blood fibronectin level throughout 4 weeks starting from the 3d day of the disease and a significantly higher fibronectin content on the 28th day as compared with that on the 3d day is likely to mirror the activity of repair processes occurring in the myocardium.  相似文献   

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