流式细胞术分析户尘螨致敏的嗜碱性粒细胞活化试验及其临床意义

目的建立流式细胞术(FCM)分析嗜碱性粒细胞活化试验(BAT)的方法并评估其在过敏性疾病诊断中的意义。方法以CD63/CD203c/CD45抗体组合,建立用FCM分析户尘螨致敏的嗜碱性粒细胞脱颗粒的方法。以皮肤点刺试验(SPT)作为金标准方法,将FCM检测活化嗜碱性粒细胞与荧光酶联免疫吸附试验(FELISA)测定特异性IgE(sIgE)结果作比较,分析其临床应用价值。结果以CD45和CD203c联合设门,可以得到纯的嗜碱性粒细胞;CD63是活化嗜碱性粒细胞的最佳标志;Spearman相关系数显示,sIgE的Unicap分级与活化嗜碱性粒细胞呈中度相关;FCM检测活化嗜碱性粒细胞与FELISA测定sIgE结果在户尘螨过敏性疾病诊断中的差异无统计学意义(P>0.05),但前者的敏感度、符合率、阳性预测值以及阳性似然比各项指标都优于后者。结论通过FCM分析CD63的表达来确定活化嗜碱性粒细胞是诊断速发型超敏反应的一种有效、安全的体外检测方法。     

流式细胞术分析户尘螨致敏的嗜碱性粒细胞活化试验及其临床意义

目的建立流式细胞术（FCM）分析嗜碱性粒细胞活化试验（BAT）的方法并评估其在过敏性疾病诊断中的意义。方法以CD63/CD203c/CD45抗体组合,建立用FCM分析户尘螨致敏的嗜碱性粒细胞脱颗粒的方法。以皮肤点刺试验（SPT）作为金标准方法,将FCM检测活化嗜碱性粒细胞与荧光酶联免疫吸附试验（FELISA）测定特异性IgE（sIgE）结果作比较,分析其临床应用价值。结果以CD45和CD203c联合设门,可以得到纯的嗜碱性粒细胞;CD63是活化嗜碱性粒细胞的最佳标志;Spearman相关系数显示,sIgE的Unicap分级与活化嗜碱性粒细胞呈中度相关;FCM检测活化嗜碱性粒细胞与FELISA测定sIgE结果在户尘螨过敏性疾病诊断中的差异无统计学意义（P〉0.05）,但前者的敏感度、符合率、阳性预测值以及阳性似然比各项指标都优于后者。结论通过FCM分析CD63的表达来确定活化嗜碱性粒细胞是诊断速发型超敏反应的一种有效、安全的体外检测方法。     

幼儿急性嗜碱性粒细胞白血病1例

正嗜碱性粒细胞白血病(basophilic cell leukemia,ABL)是一种罕见的造血系统恶性肿瘤,约占急性髓系白血病(acute myeloid leukemia,AML)的1%[1]。国内外可见少量成人病例报道,但幼儿病例极少见。现报道我院诊治的1例幼儿ABL。1病历资料患儿男,1岁8月,主因间断发热、乏力1月,发热、乏力加重伴咳嗽4 d,于2016年7月2日收住我院。患儿于入院     

流式细胞术计数外周血嗜碱性粒细胞

目的建立流式细胞术计数外周血嗜碱性粒细胞的实验方法。方法采用流式细胞术，根据嗜碱性粒细胞表面抗原的特性Lin1^-，CD123^＋，HLA-DR^-，计数了30例健康体检者及10例慢粒急变患者外周血嗜碱性粒细胞，同时用血细胞分析仪，人工涂片计数嗜碱性粒细胞。结果流式细胞术计数方法在低值（0.26％）和高值（4．27％）的精密度分别为CV=5．3％（n=10）和CV=4．1％（n=10）。在30例健康体检者中，流式细胞仪法与自动血细胞分析仪法计数嗜碱性粒细胞结果有显著差异，（P〈0．01，r=0．003）。10例慢粒急变患者流式细胞仪计数嗜碱性粒细胞与人工血涂片分类比较无显著差异且相关良好，（P〉0．05，r=0．918）。结论流式细胞仪法计数外周血嗜碱性粒细胞结果准确，可作为标准的计数嗜碱性粒细胞方法。     

嗜碱性粒细胞白血病1例报导

急性部分分化原粒细胞白血病伴嗜碱性前体粒细胞（AML—M2-BaSo）,为急性嗜碱性粒细胞白血病（Acute basophilic leukemia．AbasoL）,为一种原发性罕见白血病。     

嗜碱性粒细胞白血病的诊断与治疗进展

嗜碱性粒细胞增多常见于慢性髓细胞白血病(CML)加速期或急变期,其变化可作为CML的预后评价指标.嗜碱性粒细胞白血病(BL)是以嗜碱性粒细胞异常增多为主要临床表现的独立白血病类型.临床在排除其他血液系统疾病后,依据骨髓检查及外周血检查结果将嗜碱性粒细胞异常增多引起的BL分为急性BL和慢性BL.因BL发病率较低,目前尚无明确的诊断标准.随着检测技术和诊疗水平的不断提高,基于形态学、免疫学、细胞及分子遗传学(MICM)的诊断模式,可使越来越多单独发病的BL被识别和发现.笔者拟就目前国内外发现的BL的诊断和治疗进展进行综述.     

多发伤患者胸水出现嗜酸性和嗜碱性粒细胞增多一例并文献复习

胸腹水涂片出现嗜酸性粒细胞增多并不少见,但外伤患者出现胸水嗜酸性粒细胞并嗜碱性粒细胞增多的报道甚少,我院遇到一例(见附图),现结合文献,报道如下。     

活化嗜碱粒细胞在变应性鼻炎患者外周血中的表达

目的探讨嗜碱粒细胞在变应性鼻炎患者外周血中数量及活化率的变化及其临床意义。方法以化学诱导趋向性受体单抗作为特异性的荧光标记,采用三色免疫荧光标记流式细胞术对20例急性发作期变应性鼻炎患者(急性组)、20例反复发作变应性鼻炎患者(慢性组)及30例健康者(对照组)的外周血嗜碱粒细胞及其活化率进行定量检测。结果慢性组外周血化学诱导趋向性受体及嗜碱粒细胞高于急性组(P<0.01)和对照组(P<0.05),对照组高于急性组(P<0.01)。慢性组与急性组嗜碱粒细胞活化率均高于对照组(P<0.01)。结论嗜碱粒细胞活化是导致变应性鼻炎症状出现的主要因素之一。慢性期患者嗜碱粒细胞的持续增高和活化可能与变应性鼻炎反复发作有关。     

急性早幼粒细胞白血病伴嗜碱性粒细胞增多1例

目的报道1例急性早幼粒细胞白血病伴嗜碱性粒细胞增多。方法回顾性分析我院收治的1例伴嗜碱性粒细胞增多的急性早幼粒细胞白血病的病历资料,并对相关文献进行复习。结果一男性患者以全血细胞减少入院,结合骨髓细胞化学染色、流式细胞仪检测、染色体核型分析、融合基因等,诊断为急性早幼粒细胞白血病。但同时在骨髓细胞形态及流式细胞图形中发现嗜碱性粒细胞数量增多。结论伴特殊类型细胞增多的急性早幼粒细胞白血病的实验室诊断需结合多项实验室检查,根据流式细胞术的免疫表型分析有助于从大量异常细胞中区分出少量特殊类型细胞。     

南昌地区气传野苋菜花粉对过敏性患者血清免疫球蛋白的影响

目的:了解环境因素中野苋菜花粉对南昌地区过敏性疾病的作用。方法:选择632例临床诊断为过敏性疾病的患者,用野苋菜花粉及春、夏、秋、冬四季花粉的提取液做皮试,并测定其总免疫球蛋白E(IgE)。结果:野苋菜花粉皮试阳性为358例(56.6%);春季花粉阳性为308例(48.7%);夏季花粉阳性为316例(50.0%);秋季花粉阳性为346例(54.8%);冬季花粉阳性为342例(54.1%)。皮试总阳性率为52.9%。野苋菜花粉皮试阳性和阴性患者间的总IgE含量有明显差异(P<0.01)。结论:野苋菜花粉是南昌地区过敏性疾病的主要变应原。     

Influence of the diluent on the effect of highly diluted histamine on basophil activation

BACKGROUND: In modern pharmaceutical practice, it is common to use purified ethanol and purified water for the preparation of homeopathic dilutions. Hahnemann in 1827 recommended good brandy as a diluent. Brandy contains a lot of accompanying substances in addition to ethanol. PURPOSE OF THE STUDY: The research question was whether different diluents influence the effectiveness of high dilutions, especially above Avogadro's number. We compared two dilution media to investigate the diluent's influence. Within the limitations of the test-system, the dilution media were as similar to good brandy as possible and like purified ethanol. Dilutions of histamine were prepared with both media. As test-system, we used modified basophil activation in an in vitro cell system. Basophils are activated by anti-immunoglobulin E (anti-IgE). The activation of basophils is inhibited by prior incubation with histamine. The reduction in activation was measured with different dilutions of histamine. The test system used a 3-colour flow cytometric method. The interleukin-3 (IL-3) receptor CD123 was used to identify basophils in the leukocyte mixture. The CD63 surface marker was used for quantification of activated basophils. RESULTS: With higher concentrations of histamine, we observed inhibition on optimally anti-IgE-stimulated basophil activation with a clear concentration dependency. With low concentrations of histamine (up to 10(-31)), we also observed inhibition of IgE-mediated basophil activation. Differences were observed between the dilution media. CONCLUSION: The preliminary results support the hypothesis that the dilution medium may influence the effects of high dilutions. This could be of importance for homeopathic pharmaceutical practice as well as for ultra-high dilution experiments. The refined basophil test system proved to be highly sensitive and reliable. Further studies are needed.     

Spirometry and ventilation-perfusion inequality in patients with mild allergic asthma before and during the pollen season

Summary. Several studies on asthma have shown a low correlation between gas exchange and spirometry, especially after treatment with bronchodilators. The aim of the present study was therefore to examine both spirometry results and gas exchange during a pollen-free period and at the end of the pollen season in patients with mild and well-controlled allergic asthma. Pulmonary gas exchange was studied using a modified form of the multiple inert gas elimination technique. Lung volumes and forced expiratory flows were measured by common spirometry. During the non-pollen season, spirometry and forced expiratory flows were within the reference values in all but one patient, who had decreased indices for airway flow. Three other patients showed signs of minor gas exchange impairment. During the pollen season, FRC was slightly increased (P<0.05) and MEF₅₀ was slightly decreased (P<0.05) for the group. Two patients had an increased index for gas exchange impairment (log SDQ was 0.64 and 0.59) and four patients had borderline log SDQ (0.50 to 0.56). However, the mean log SDQ was not increased in the pollen season. The results show that, both in the pollen season and in the pollen-free season, low degrees of gas exchange impairment could be present in pollen allergic asthmatic patients despite normal spirometry. The low degree of gas exchange impairment in some patients indicates the presence of airway inflammation with oedema and/or secretion. However, high degrees of ventilation-perfusion inequality were not observed in these patients where air flow rates were mainly normal.     

精神科护士工作场所受欺负状况调查

目的调查精神科护士工作场所受欺负的现状。方法使用中文版负性行为问卷对重庆市精神卫生中心的护士共150人进行调查。结果被调查者有13.4%在工作场受欺负,严重受欺负人群占3.2%。不同学历、职称、年龄、婚姻状况、工龄的护士NAQ-R评分比较差异有统计学意义(P0.05)。结论工作场所受欺负存在于精神科护士中,且受年龄、职称、学历等因素影响,应引起护理管理者的重视。