1936例儿童外阴阴道炎症状与病原体分析

目的：分析儿童外阴阴道炎的临床表现与病原体构成。方法：观察1936例患有外阴阴道炎儿童的临床表现,取外阴阴道分泌物进行革兰氏染色涂片、支原体培养、淋病奈瑟菌培养、念珠菌培养和沙眼衣原体抗原检查。结果：儿童外阴阴道炎主要症状有外阴红斑、瘙痒、疼痛、阴道分泌物增多。病原学检查阳性结果分别是1782例细菌＼442例支原体、157例念珠菌、32例淋病奈瑟菌;8例同时培养出念珠菌和支原体;23例沙眼衣原体抗原阳性;154例无病原体。结论：儿童外阴阴道炎表现多样,从外阴阴道分泌物中可检测出细菌、淋病奈瑟菌、衣原体、支原体、念珠菌等病原体,应根据检测结果使用合适药物以提高治愈率。     

细菌性皮肤病

20 0 30 0 5 3　 12 0 0例妇科门诊病人细菌性阴道病检测报告 /李亚伟 (山东枣庄皮防院 )…∥中国麻风皮肤病杂志 .- 2 0 0 2 ,18(2 ) .- 137～ 138按常规取宫颈和阴道分泌物作检查。结果 :检测阴道加德纳菌 2 84例 (2 3.6 7% ) ,念珠菌 35 6例(2 9.6 7% ) ,支原体 313例 (2 6 .0 8% ) ,滴虫 15 9例(13.2 5 % ) ,淋球菌 88例 (7.33% )。细菌性阴道病合并感染的病原体念珠菌 6 2例 (2 1.83% ) ,支原体 4 8例 (16 .90 % ) ,滴虫 14例 (4 .93% ) ,念珠菌+支原体 13例 (4 .5 8% ) ,念珠菌 +滴虫 4例(1.4 1% ) ,滴虫 +支原体 11例 (3.87% ) ,淋球…     

1180例孕妇性传播疾病检查结果分析

目的：了解性传播疾病在孕妇中的流行情况。方法：对1180例入院我院待产的孕妇，取阴道分泌物革兰氏染色找念珠菌、滴虫；取宫颈分泌物作沙眼前原体抗原检测及淋球菌、支原体培养。结果：1180例孕妇标本病原体检出率为17．5％，检出率依次为淋球菌0．34％，滴虫0．68％，念珠菌3．22％，沙眼衣原体5．76％，支原体7．46％。结论：由衣原体、支原体感染引起的非淋菌性宫颈炎是孕妇最常见的性传播疾病。     

老年妇女性传播疾病病原体检测分析

目的了解老年妇女性传播疾病病原体感染情况。方法对患者分别进行淋球菌、沙眼衣原体、解脲脲原体、人型支原体、滴虫和念珠菌检测。结果386例患者共检出淋球菌3例(0.78%)、沙眼衣原体6例(1.55%)、解脲脲原体107例(27.72%)、人型支原体4例(1.04%)、滴虫19例(4.92%)、念珠菌63例(16.32%),其中解脲脲原体和念珠菌阳性率均明显高于健康对照组,经χ2检验,差异具有显著性(P<0.01)。结论老年妇女性传播疾病病原体以解脲脲原体为主,念珠菌亦是常见的主要病原体之一。     

儿童泌尿生殖道沙眼衣原体、解脲脲原体的检测及其意义

目的 探讨儿童泌尿生殖道沙眼衣原体（Ct）、解脲脲原体（Uu）等病原体的感染情况。方法 对门诊就诊的155例儿童，取尿道分泌物、阴道分泌物检测炎症细胞、Ct、Uu、淋球菌（Ng）、念珠菌、阴道毛滴虫、蛲虫卵。结果 22例女童阴道分泌物检出白细胞（WBC）阳性，Q检出率6．45％（10／155），Uu检出率22．58％（35／155），检出淋球菌2例（1．29％），念珠菌2例（1．29％），蛲虫卵3例（1．94％）。结论 对儿童泌尿生殖道炎症，要注意查找性病因素。儿童泌尿生殖道内存在Ct、Uu健康携带或正常定植情况。     

男性尿道分泌物1314份病原谱分析

目的分析男性尿路感染的病原谱,探讨防止男性尿路感染慢性化的途径。方法对男性尿道分泌物进行湿片及干片染色镜检和尿常规分析,明确有无尿道感染及病原体种类,检测滴虫和白念珠菌等;以细菌培养法检测淋球菌、加德纳菌、金黄色葡萄球菌、白念珠菌等致病菌,以免疫金标法检测衣原体等;按病原体的种类和种数进行数据统计。结果 1314例尿道分泌物共检出病原体12类2084株,根椐检出的病原体种类可将尿道炎细分为9种。多数急性尿道炎是由淋病奈瑟菌、支原体和衣原体等单一感染引起(580/736),菌株数为946株;由MCP、加德纳菌等多重感染引起的慢性尿道炎有431例,菌株数为1124株。两者病原体株数差异有统计学意义(P<0.01)。部分病例有5种以上病原体感染,部分病例镜检白细胞、红细胞、上皮细胞数明显异常,但培养均未检出细菌。结论急、慢性尿道炎的病原体有明显差异,尿道分泌物的病原谱分析可为临床正确诊断、治疗尿道炎症提供依椐。慢性尿道炎病例可出现MUBV现象及ASU病例。     

阴道异常分泌物702例检测结果分析

目的探讨锡盟地区STD门诊女性阴道异常分泌物的病原学特点。方法对本所STD门诊5年来的阴道分泌物异常为主要表现的702例患者进行涂片检查。结果阴道分泌物病原菌检出率为100%,其中淋球菌168例(26.42%),滴虫412例(53.36%),白念珠菌517例(73.24%),加特纳菌516例(74.63%)。结论造成女性阴道分泌物异常的病原菌主要是阴道滴虫、白念珠菌、加特纳菌。     

85例泌尿生殖道支原体、衣原体检测情况报告

为了解泌尿生殖道感染患者衣原体及支原体的感染情况 ,我们对本院性病门诊的 85例非淋菌性尿道 (宫颈 )炎及淋病患者作沙眼衣原体、解脲支原体检测。1　资料和方法1 1　ＮＧＵ组　 5 2例患者均按非淋菌性尿道 (宫颈 )炎的诊断标准〔1〕,其中男 35例 ,女 17例 ,年龄 18～ 5 0岁。全部病例分泌物涂片及培养都未检出淋球菌。1 2　淋病组　 33例患者分泌物涂片及培养均检出淋球菌 ,其中男 2 2例 ,女 11例。1 3　取材　男性用无菌拭子插入尿道口内 2ｃｍ～ 3ｃｍ ,停留5秒种转动取出拭子。女性扩阴后 ,用棉签揩去宫颈外口分泌物 ,再用拭子插入宫…     

梅毒患者的其他性传播感染

为了研究伴随梅毒感染的其他病原体 ,1999- 2 0 0 0年对塔什干地区 798例 (男 2 87例 ,女 399例 ,儿童 112例 )在乌孜别克斯坦皮肤性病研究所性病科住院的患者进行了临床和细菌学检查。其中第 1组为二期复发梅毒 347例(4 3 5 % ) (男 14 6 ,女 2 0 1) ;第 2组为早期隐性梅毒 314例(39 4 % ) (男 130 ,女 184 ) ;第 3组为一期梅毒患者 137例(17 1% ) ,其中男 11例、女 14例、儿童 112例已得到预防治疗。梅毒患者根据临床和血清学诊断。患者的其他病原体诊断 :淋球菌和滴虫以宫颈、尿道和阴道分泌物涂片染色以及培养 ,泌尿生殖道沙眼衣原体用…     

452例妇女外阴瘙痒病因调查分析

目的 :　对皮肤性病科门诊妇女外阴瘙痒患者进行病因调查分析。方法 :　对以外阴瘙痒为主诉的妇女患者进行皮肤性病科临床检查 ;取阴道分泌物进行pH值、胺试验及念珠菌、滴虫和线索细胞检查 ;取宫颈分泌物作淋球菌、解脲支原体培养和沙眼衣原体荧光抗原检查 ;可疑部位取材作HSV和HPV检测及梅毒血清学检查。结果 :　在 45 2例入选患者中 ,外阴阴道念珠菌病、非淋菌性宫颈炎和细菌性阴道病分别占 2 5 .44 %、2 0 .79%和 13.5 0 % ,外阴湿疹、滴虫性阴道炎和外阴瘙痒症也较多见。结论 :　与性传播因素有关的病原体感染及外阴湿疹和瘙痒等是皮肤性病科就诊妇女外阴瘙痒的主要原因。     

Prevalence of asymptomatic sexually transmitted infections among high-risk patients attending a free anonymous HIV-screening center

INTRODUCTION: The aim of this study was to assess the prevalence of sexually transmitted infections among patients attending an anonymous HIV Screening Center. PATIENTS AND METHODS: This prospective study was performed in the HIV Screening Center of University hospital in Reims (France) from May 1997 to December 1997. The inclusion criteria were the asymptomatic clinical presentation and the presence of risk factors for sexually transmitted infections referring to WHO criteria. The methods included clinical examination after application of acetic acid and urethral and endocervical swabs to identify:Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Gardnerella vaginalis, Trichomonas vaginalis in specific culture. Treponema pallidum and HIV-1 infection were both detected by Enzym Linked Immuno Sorbent Assay (ELISA). RESULTS: One hundred and one patients (62 men and 39 women) were included in the study. Their mean age was 27 +/- 4 Years. Risk factors for sexually transmitted infections were: multiple sexual partners 81 p. 100; homo or bisexuality 16 p. 100; intravenous drug use 3 p. 100. The sexually transmitted infections were: HIV-1 infection 1 p. 100;Ureaplasma urealyticum 25 p. 100; genital warts 5 p. 100;Chlamydia trachomatis 3 p. 100; Gardnerella vaginalis 3 p. 100; Mycoplasma hominis 2 p. 100; Treponema pallidum 0 p. 100; Neisseria gonorrhoeae 0 p. 100; Trichomonas vaginalis 0 p. 100. The prevalence of sexually transmitted infections was significantly higher among women (p<0.05). DISCUSSION: Classical sexually transmitted infections and HIV infection were rarely detected in this study; but prevalence of other sexually transmitted infections (genital warts, Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum) was high. Ureaplasma urealyticum is considered as a possible pathogenic agent in pregnant women (preterm delivery, decrease of birth weight, chorioamniotitis). These results suggest that other than sexually transmitted infections in high risk patients attending a HIV Screening Center other sexually transmitted infections should also be systematically screened for.     

Nonspecific vaginitis and other genital infections in three clinic populations

Nonspecific vaginitis was present in 37% of 40 patients attending the Special Urology (Sexually Transmitted Disease) Clinic at the Victoria General Hospital, in 23% of 75 patients attending the Family Planning Clinic, and in 23% of 13 patients attending the Prenatal Clinic at the Grace Maternity Hospital in Halifax, Nova Scotia. The mean prevalence was 27%. Subjective complaints of vaginal odor were significantly associated with nonspecific vaginitis (P less than .001), but symptoms of vaginal discharge or vulvar irritation and itching were not. No correlations were found between the presence of nonspecific vaginitis and the isolation of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, Trichomonas vaginalis, or vaginal yeast species. The organic acid present in vaginal washings that best correlated with the presence of nonspecific vaginitis was succinic acid.     

Ciprofloxacin treatment of chlamydial infections of urogenital tracts of women.

Ciprofloxacin was evaluated in chlamydial infections of the urogenital tracts of women treated with a dosage regimen of 500 mg orally twice a day for seven days. Of the 40 women evaluated, 30 were infected with Chlamydia trachomatis only, two were infected with Neisseria gonorrhoeae only, and a further eight had combined gonococcal and chlamydial infections. Ten were found to be harbouring Chlamydia trachomatis in the urethra as well as the cervix. Neisseria gonorrhoeae was eradicated from all patients with or without concomitant chlamydial infection. The overall chlamydial reisolation rates were 14% (5/35) four weeks after treatment and 23% (6/26) 11 weeks after treatment. The organism was not reisolated from the urethra of any of the patients after treatment. Ciprofloxacin was effective against Mycoplasma hominis, but almost completely ineffective against Ureaplasma urealyticum.     

Colonization of sexually abused children with genital mycoplasmas

Although sexually abused children are usually evaluated only for the presence of infections with Neisseria gonorrhoeae and Treponema pallidum, they are also at risk for acquiring other sexually transmitted organisms prevalent in the adult population. Accordingly, we examined pharyngeal, anorectal and genital specimens from 50 children who had been sexually abused and from 40 healthy children who served as controls; these specimens were cultured for Mycoplasma hominis and Ureaplasma urealyticum. M. hominis was isolated from the anorectal and vaginal cultures of 11 (23%) and 16 (34%), respectively, of 47 abused girls as compared with three (8%) and six (17%), respectively, of 36 controls. U. urealyticum was isolated from the anorectal and vaginal cultures of nine (19%) and 14 (30%), respectively, of the abused girls as compared with one (3%) and three (8%), respectively, of 36 controls. Colonization with genital mycoplasmas was not associated with any symptoms.     

Women at risk for gonorrhea: comparison of rosaramicin and ampicillin plus probenecid in the eradication of Neisseria gonorrhoeae, Chlamydia trachomatis and genital mycoplasmas

Rosaramicin is a macrolide antibiotic with activity against Neisseria gonorrhoeae, Chlamydia trachomatis, and the genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis. Its efficacy in the treatment of genital infections was evaluated and compared with that of single-dose ampicillin plus probenecid in women with known, or suspected, uncomplicated gonococcal infection. Isolation rates for N. gonorrhoeae, C. trachomatis, U. urealyticum, and M. hominis were 72%, 44%, 95%, and 65%, respectively. Rosaramicin cured 24 (89%) of 27 gonococcal infections and 11 (92%) of 12 chlamydial infections. It transiently reduced the carriage of U. urealyticum but had little effect on carriage of M. hominis. Rosaramicin may be of value in the treatment of concurrent gonococcal and chlamydial infections when tetracycline is contraindicated.     

The co-existence of genital Mycoplasma and Neisseria gonorrhoeae isolated from the male urethra

Ureaplasma urealyticum and Mycoplasma hominis, separately or together, were co-isolated along with 34 of 102 strains of Neisseria gonorrhoeae cultured from urethral swabs from men with urethritis. For approximately half of the N. gonorrhoeae strains, the mycoplasma(s) persisted for at least five passages on agar medium. U. urealyticum was isolated in 31 of the 34 instances. No association between particular serotype(s) of U. urealyticum or auxotypes of N. gonorrhoeae was identified. The auxotypes of the N. gonorrhoeae isolates were not altered by the presence of U. urealyticum. To screen cultures of N. gonorrhoeae for the presence of genital mycoplasmas, we recommend direct microscopy of growth on agar: for M. hominis, after the colony epifluorescence test, and, for U. urealyticum, after the urease spot test.     

性病病征处理的临床疗效观察

目的 探讨病征处理对淋球菌和沙眼衣原体感染的诊断准确性和临床疗效。方法 选择具有尿道分泌物和阴道分泌物异常的性病门诊初诊患者，对其进行病征处理，同时进行病原学诊断。结果 624例患者中男290例，女334例，病征诊断对淋球菌和沙眼衣原体感染总的敏感性、特异性和阳性预期值分别为91．3％、45．2％和67．0％。随访患者的治愈率为72．2％，总有效率为91．3％。女性患者的治愈率显著低于男性，副作用少见。结论 具有尿道分泌物的男性患者应用病征处理的原则进行治疗，治愈率高、安全快捷。对具有阴道分泌物异常的女性患者来说，病征处理有一定疗效，但还不令人满意。     

Correlation of ultrasonography and bacteriology of the endocervix and posterior cul-de-sac of patients with severe pelvic inflammatory disease

We evaluated 30 patients admitted with a diagnosis of pelvic inflammatory disease (PID) by culdocentesis and ultrasonography to determine the severity of disease in relation to endocervical and culdocentesis bacteriology. Specimens from the endocervix were cultured for Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum. In addition, culdocentesis fluid was cultured for aerobes and anaerobes. Patients with N. gonorrhoeae or C. trachomatis had significantly milder disease (P less than 0.05) compared with patients whose specimens were negative for those organisms. Furthermore, those patients with ultrasound confirmation of a tubo-ovarian complex had significantly (P less than 0.0001) greater recovery of aerobes and anaerobes from culdocentesis fluid than did patients with milder disease.     

Sexually transmitted and reproductive tract infections in symptomatic clients of pharmacies in Lima, Peru

OBJECTIVE: To determine prevalences and predictors of sexually transmitted and reproductive tract infections among men and women seeking care at pharmacies. METHODS: Men and women with urethral discharge or dysuria and vaginal discharge were enrolled at 12 central and 52 smaller pharmacies in Lima, Peru. All participants answered a questionnaire. Men provided urine for polymerase chain reaction (PCR) testing for Neisseria gonorrhoeae and Chlamydia trachomatis, and for leucocyte esterase testing. Women provided self-obtained vaginal swabs for PCR testing for N gonorrhoeae and C trachomatis, Trichomonas vaginalis culture and bacterial vaginosis and Candida. RESULTS: Among 106 symptomatic men, N gonorrhoeae and C trachomatis were detected in 34% and were associated with urethral discharge compared with dysuria only (odds ratio (OR) 4.3, p = 0.003), positive urine leucocyte esterase testing (OR 7.4, p = 0.009), less education (OR 5.5, p = 0.03), and with symptoms for <5 days (OR 2.5, p = 0.03). Among 121 symptomatic women, 39% had bacterial vaginosis or T vaginalis, and 7.7% had candidiasis. N gonorrhoeae and C trachomatis were detected in 12.4% of the women. Overall, 48.8% had one or more of these infections. No factors were associated with vaginal infection, and only symptoms of vaginal discharge for <5 days were associated with N gonorrhoeae and C trachomatis (OR 4.0, p = 0.02). The main reason reported for seeking advice at pharmacies by both men and women was trust in pharmacy workers. CONCLUSIONS: Among men and women presenting to pharmacies with urethral and vaginal symptoms, rates of urethral and vaginal infections were comparable to those found in other clinical settings. Pharmacies can contribute to the care and prevention of sexually transmitted infection in developing countries.