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1.
解脲脲原体(Uu)和人型支原体(Mh)是引起泌尿生殖道感染的主要病原体,近年来由其引起的泌尿生殖道感染的发病率不断上升.加上抗生素的不规则应用,支原体耐药株日渐增多。为了解2001年昆明地区Uu、Mh的感染状况及其对抗生素的敏感性,笔者检测了2065例可疑的支原体感染者,现将结果报告如下。  相似文献   

2.
泌尿生殖道支原体的检测和药敏分析   总被引:21,自引:0,他引:21  
目的:了解沈阳地区2000-2002年泌尿生殖道分泌物中分离的解脲脲原体(Uu)和人型支原体(Mh)的药物敏感情况。方法:应用Mycoplasma IST试剂盒对Uu和Mh进行检测,并对6种抗菌药物的药敏试验进行分析。结果:6966例可疑泌尿生殖道感染患者中,2365例检出支原体,其中Uu 1589例,Mh 154例,Uu Mh 622例。3组检出的支原体对抗菌药物敏感性由高到低依次为Uu、Mu、Uu Mh。3组检出的支原体对多西环素、交沙霉素、原始霉素敏感性均很高,对氧氟沙星的敏感性较低。支原体对6种抗菌药物的敏感性逐年下降,出现对6种抗菌药物均耐药的支原体。结论:支原体对抗菌药物的耐药性逐年增加。支原体药敏试验可以指导合理用药,减少耐药株的产生。  相似文献   

3.
泌尿生殖道支原体感染已引起重视[1,2],支原体的耐药谱也不断变化[3,4],为了解泌尿生殖道解脲脲原体(Uu)和人型支原体(Mh)在本地区的感染率及其耐药状况,对来我院就诊的可疑患者进行支原体培养、计数及药敏试验,以供临床泌尿生殖道支原体感染患者选用药物参考.现将结果报告如下.  相似文献   

4.
目的:研究健康学龄前儿童泌尿生殖道解脲脲原体(Uu)及人型支原体(Mh)分子流行病学特征.方法:对408份健康学龄前儿童尿液标本进行Uu、Mh培养,阳性者用聚合酶链反应(PCR)进一步确证,并对扩增阳性的Uu进一步分群与分型.结果:408例标本中,Uu阳性27例(6.62%);Mh阳性3例(0.74%).Uu单纯生物一群占48.15% (13/27),单纯生物二群占29.63%(8/27),两群混合占22.22% (6/27).Uu阳性率男性为4.72% (12/254),女性为9.74% (15/154),差异有统计学意义(x2=3.903,P=0.048).农村儿童Uu阳性率为10.83% (13/120),城市儿童为4.86%(14/288),差异有统计学意义(x2=4.889,P=0.027).结论:Uu与Mh可能属于健康学龄前儿童泌尿生殖道中的正常定植菌群.  相似文献   

5.
目的 探讨解脲脲原体(Uu)和人型支原体(Mh)在健康中小学生泌尿生殖道中的定植情况,以及Uu的分群分型状况.方法 培养法对青岛地区957名中小学生的尿沉渣进行Uu和Mh培养,培养阳性者用PCR确证,并对Uu阳性标本进一步分群分型.结果 Uu阳性46例,占4.81%,生物一群占69.57%,生物二群30.43%.小学生Uu阳性12例,占2.97%,生物一群9例(75.00%),生物二群3例(25.00%).初中生Uu阳性8例,占3.08%:生物一群5例(62.50%),生物二群3例(37.50%).高中生Uu阳性26例,占8.87%:生物一群18例(69.23%),生物二群8例(30.77%).Mh阳性9例(0.94%),其中小学生Mh阳性1例,初中生Mh阳性1例,高中生Mh阳性7例.3个组Uu阳性率女生均高于男生(P<0.05).结论 Uu和Mh在中小学生泌尿生殖道中存在无症状的携带状态.尽管各年龄组检出的Uu均以生物一群为主,但生物二群也占有不小的比例,提示Uu可能是健康中小学生人群正常定植菌,且女性携带率高于男性.  相似文献   

6.
为了解沿海开放城市沙眼衣原体(Ct)、解脲支原体(Uu)、人型支原体(Mh)所致NGU的感染情况,我们对汕头市皮肤病医院2001年度性病门诊4413例泌尿生殖道炎患者标本检测结果进行了分析,现报道如下。  相似文献   

7.
沙眼衣原体(Q)及解脲支原体(Uu)和人型支原体(Mh)是非淋菌性尿道炎的主要病原体,由此感染所引起的前列腺炎、宫颈炎、输卵管炎及其后遗症——不孕不育日益增多。我们对生殖门诊就诊患者217例的泌尿生殖道标本进行检测,现报道如下。  相似文献   

8.
A7固体和液体培养检测泌尿生殖道支原体的效果评价   总被引:1,自引:0,他引:1  
目的探讨A7琼脂固体和液体培养在检测泌尿生殖道标本中解脲脲原体(UU)和人型支原体(MH)的意义,并评价两种方法的优缺点,为临床诊断UU和MH的感染提供更准确的实验依据。方法临床标本洗脱于液体培养肉汤后,直接接种于液体培养鉴定板条和A7琼脂固体平板,分别在37℃温箱和含5%CO237℃温箱中培养24h和48h后观察结果;剩余肉汤经12h增菌后接种于A7平板,在5%CO237℃温箱中培养48~72h后观察结果。结果422例临床标本,液体培养法阳性199例,未经增菌固体培养阳性158例,增菌后固体培养阳性169例。增菌后固体培养与液体培养、未经增菌固体培养分别比较(P均<0.005),差异均有显著性。结论A7琼脂固体培养比液体培养更具优势,两种方法的联合应用,取长补短,能为临床诊断提供更准确实验数据。  相似文献   

9.
为分析近几年性病门诊就诊者中沙眼衣原体(Ct)和解脲脲原体(Uu)感染的流行趋势和特征。我们收集2001~2004年本院性病门诊病例,并对泌尿生殖道分泌物检测者的人口学特征和检测结果等资料进行统计分析。4年中共检测18727例,阳性7135例(38.10%),其中Ct阳性2066例(11.03%),Uu阳性4072例(21.74%),Ct Uu阳性576例(3.08%),Ct、Uu与NG混合感染506例(2.71%),Ct、Uu在19~40岁性活跃年龄段人群感染率较高,男性泌尿生殖道以Ct感染、女性泌尿生殖道以Uu感染较多,在性病防治及监测中应引起重视。  相似文献   

10.
泌尿生殖道支原体检测及药敏试验分析   总被引:2,自引:0,他引:2  
为了解支原体在泌尿生殖道中的感染及耐药情况。我们对769例疑似NGU患者进行支原体培养、鉴定、药敏一体化试剂盒进行检测。结果支原体培养阳性178例,阳性率23.1%,其中解脲支原体(Uu)阳性143例(80.3%);人型支原体(Mh)阳性9例(5.1%);Uu和Mh混合感染26例(14.6%)。Uu对10种药物作药敏试验,敏感性最高者为米诺环素(93.7%),耐药率最高者为四环素(32.2%)。米诺环素是本地区治疗泌尿生殖道支原体感染的首选药物?  相似文献   

11.
Ureaplasma urealyticum and Mycoplasma hominis, separately or together, were co-isolated along with 34 of 102 strains of Neisseria gonorrhoeae cultured from urethral swabs from men with urethritis. For approximately half of the N. gonorrhoeae strains, the mycoplasma(s) persisted for at least five passages on agar medium. U. urealyticum was isolated in 31 of the 34 instances. No association between particular serotype(s) of U. urealyticum or auxotypes of N. gonorrhoeae was identified. The auxotypes of the N. gonorrhoeae isolates were not altered by the presence of U. urealyticum. To screen cultures of N. gonorrhoeae for the presence of genital mycoplasmas, we recommend direct microscopy of growth on agar: for M. hominis, after the colony epifluorescence test, and, for U. urealyticum, after the urease spot test.  相似文献   

12.
Rosaramicin is a macrolide antibiotic with activity against Neisseria gonorrhoeae, Chlamydia trachomatis, and the genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis. Its efficacy in the treatment of genital infections was evaluated and compared with that of single-dose ampicillin plus probenecid in women with known, or suspected, uncomplicated gonococcal infection. Isolation rates for N. gonorrhoeae, C. trachomatis, U. urealyticum, and M. hominis were 72%, 44%, 95%, and 65%, respectively. Rosaramicin cured 24 (89%) of 27 gonococcal infections and 11 (92%) of 12 chlamydial infections. It transiently reduced the carriage of U. urealyticum but had little effect on carriage of M. hominis. Rosaramicin may be of value in the treatment of concurrent gonococcal and chlamydial infections when tetracycline is contraindicated.  相似文献   

13.
Vaginal colonization with mycoplasma hominis and ureaplasma urealyticum   总被引:5,自引:0,他引:5  
Vaginal cultures obtained from unselected young women who consulted the gynecologist in a student health service were examined for Ureaplasma urealyticum and Mycoplasma hominis. Each participant completed a confidential questionnaire. Multiple logistic regression analysis was used to determine which variables, of a large number ascertained, were associated with mycoplasmal colonization. U. urealyticum was isolated from 273 (56.8%) of 481 participants. The following variables were significantly predictive of colonization with U. urealyticum: black race, absence of antibiotic use, cigarette smoking, and number of sexual partners during the last year. Lifetime number of sexual partners was significantly predictive only in women who used nonbarrier methods of contraception. M. hominis was isolated from 85 (17.7%) of the 481 participants. Independent variables that were significantly predictive of colonization with M. hominis included black race, young age, and, for users of nonbarrier methods of contraception, lifetime number of sexual partners.  相似文献   

14.
Clinical and experimental investigations on the significance of Mycoplasma hominis and Ureaplasma urealyticum have revealed different and contradictory results. Both germs are frequently discovered in young, sexually active persons. Ureaplasma urealyticum might be the cause of some cases of non-gonococcal urethritis. M. hominis seems to be one causative agent of endometritis, salpingitis, parametritis and septicaemia after birth; we do not know yet, however, how often this may be the case. M. hominis may also infect the newborn, e.g., it may cause meningitis and encephalitis. The diagnosis of an infection with mycoplasmas is mainly based on the isolation of the organism, the lack of other pathogens in the lesions, and the demonstration of a significant change of titer of homologous antibodies. Tetracycline is the drug of choice; alternatives are clindamycin for M. hominis and erythromycin for U. urealyticum.  相似文献   

15.
Urethritis due to Chlamydia trachomatis.   总被引:4,自引:2,他引:2       下载免费PDF全文
Ninety-five men suffering from gonococcal urethritis were treated and observed. Forty-nine developed postgonococcal non-specific urethritis (PGU). Seventeen men were demonstrated to be free from PGU after careful observation; these formed a control group. Chlamydia trachomatis was isolated from urethral material from 26 (53%) of the PGU group but from none of the controls. This difference was highly significant (P less than 0-001). It confirms that C. tachomatis is a pathogen in the urethra. The presence of specific IgM antibody to C. trachomatis in serum from some men developing PGU, from whom that organism was isolated, suggests that the infection was recent in those cases. Ureaplasma urealyticum (T strain mycoplasma) was isolated from urethral material taken from 22 (45%) of the 49 men in the PGU group, and from 12 (71%) of the 17 in the control group. Mycoplasma hominis was isolated from 10 (20%) of the 49 men in the PGU group, and from four (24%) of the 17 men in the control group. Thus, no evidence was obtained that mycoplasmas (U. urealyticum, M. hominis) are patogenic in the urethra.  相似文献   

16.
Twenty-two men with non-gonococcal urethritis (NGU), 19 with gonorrhoea, and 22 without urethritis were examined for various micro-organisms. Chlamydia trachomatis was isolated from the urethra of 45% of men with NGU, 21% of those with gonorrhoea, but from none without urethritis. Ureaplasma urealyticum but not Mycoplasma hominis was recovered from a larger proportion of men with NGU than from those in the other groups. M genitalium was isolated presumptively from 32% of men with NGU, 12% of those with gonorrhoea, from 10% of men without urethritis, and from 42% of the men with NGU from whom chlamydiae were not isolated. U urealyticum, M hominis, and M genitalium were sought also in the rectum of men in the three groups. The first two micro-organisms were confined almost exclusively to homosexual men, whereas M genitalium was apparently not restricted in this way and was found particularly in this site in men with NGU. The latter mycoplasma may be a resident primarily of the intestinal tract. A fourfold or greater rise in the titre of antibody to C trachomatis was detected in about 20% of the patients with NGU, but not in other men. A similar rise in the titre of antibody to M genitalium was seen in 29% of the patients with NGU and in 12% of those without urethritis. A concomitant antibody response to M pneumoniae, which is antigenically related to M genitalium, was seen in one patient only. The responses to M genitalium suggest infection by this mycoplasma and indicate the need for further serological studies.  相似文献   

17.
目的 建立PCR反向斑点杂交方法(PCR-RDB)快速鉴定泌尿生殖道常见致病性支原体.方法 以4种支原体[微小脲原体(Up)、解脲脲原体(Uu)、生殖支原体(Mg)、人型支原体(Mh)]的16SrRNA基因为靶序列设计通用引物和探针,将4种特异的寡核苷酸探针固定于尼龙膜上.利用巢式PCR扩增Up、Uu、Mg和Mh,扩增产物变性后与各特异性探针进行杂交、显色并分析结果,并对检测体系的敏感性、特异性进行测试.同时检测分析60例临床拭子标本.结果 4种特异性探针只与相应的支原体DNA杂交,与其他病原体无交叉反应,其敏感性是1 CFU.60例临床拭子标本PCR-RDB方法共筛选出支原体阳性19例,其中3例为支原体混合感染的标本(Up+Uu 2例,Uu+Mg 1例).结论 该方法可快速、敏感、准确地鉴定引起泌尿生殖道感染的致病性支原体.  相似文献   

18.
解脲脲原体与黏液脓性宫颈炎相关性的临床研究   总被引:1,自引:0,他引:1  
目的:通过分析解脲脲原体(Uu)与黏液脓性宫颈炎(MPC)的临床相关性,研究解脲脲原体对宫颈的致病性。方法:采用病例-对照研究方法。结果:有临床症状、体征的107例宫颈单纯Uu检出者与有临床症状、体征的61例宫颈Uu混合其他性病病原体检出者,两组MPC的发生率差异无显著性(OR=0.317,χ^2=3.364,P=0.067);宫颈单纯Uu检出阳性伴MPC者,宫颈Uu被清除后,MPC的痊愈率明显高于宫颈Uu未被清除者(OR=0.230,χ^2=6.90,P=0.009)。结论:Uu与MPC存在一定的相关性.Uu对宫颈有一定的致病性。  相似文献   

19.
目的:用液体培养基分离解脲支原体(UU),并电镜下观察其形态特征。方法:用选择性UU液体培养基分离尿生殖道标本中的UU,阳性结果经PCR方法证实,电镜下观察其形态。结果:阳性者为尿素酶阳性,培养液呈透明红色,PCR检测为阳性,电镜观察可见大小不等、无细胞壁的圆形、椭圆形、杆状、发芽状多形性特征的UU细胞,大小为0.03~0.12μm×0.12~0.35μm,未见细菌细胞。结论:选择性液体培养基能充分利用UU在液体培养基中生长迅速、分解尿素和缺乏细胞壁的特性,有效地抑制尿生殖道标本中的细菌生长,获得UU纯培养物。液体培养法操作简单,重复性好。  相似文献   

20.
目的:确定解脲脲原体(UU)在健康足月分娩新生儿泌尿生殖道中的检出率与分群分型。方法:UU液体培养法初筛健康足月分娩248例新生儿外阴分泌物,阳性标本用PCR法及酶切法进行分群分型。结果:248例标本中,UU阳性率为10.48%,经阴道分娩组与剖宫产组阳性率差异有统计学意义(P<0.01);男婴与女婴阳性率差异有统计学意义(P<0.01)。26例阳性标本中,生物一群18例(69.23%),生物二群6例(23.08%),混合群2例(7.69%)。生物一群中1型4例,6型10例,3/14型2例,混合型2例。结论:本研究中健康新生儿泌尿生殖道中UU以生物一群为主,主要为1型和6型。  相似文献   

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